Planaria EST Database


DrC_00600

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00600
         (578 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P32773|TOA1_YEAST  Transcription initiation factor IIA la...    38   0.021
sp|Q9N4M4|ANC1_CAEEL  Nuclear anchorage protein 1 (Anchorage...    38   0.021
sp|P25980|UBF1B_XENLA  Nucleolar transcription factor 1-B (U...    36   0.079
sp|P36044|MNN4_YEAST  Protein MNN4                                 34   0.30 
sp|P24788|CD2L1_MOUSE  PITSLRE serine/threonine-protein kina...    33   0.39 
sp|Q03101|CYAG_DICDI  Adenylate cyclase, germination specifi...    33   0.39 
sp|Q6FSW2|RAD52_CANGA  DNA repair and recombination protein ...    33   0.51 
sp|Q6DRL5|MBB1A_BRARE  Myb-binding protein 1A-like protein         33   0.67 
sp|Q960E8|TF2H1_DROME  TFIIH basal transcription factor comp...    32   0.87 
sp|P46916|YTXO_BACSU  Hypothetical protein ytxO (ORFY)             32   0.87 
>sp|P32773|TOA1_YEAST Transcription initiation factor IIA large chain (TFIIA 32 kDa
           subunit)
          Length = 286

 Score = 37.7 bits (86), Expect = 0.021
 Identities = 22/72 (30%), Positives = 43/72 (59%), Gaps = 2/72 (2%)
 Frame = +1

Query: 4   VNFLNMSTVENITPVTEEKNN--LKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSE 177
           +N  N++TVENI   +E+K++   +E +EK   + E ++  + + KK++ S L    D++
Sbjct: 160 INNANITTVENIDDESEKKDDEEKEEDVEKTRKEKEQIEQVKLQAKKEKRSAL---LDTD 216

Query: 178 EKAQNGDDAEED 213
           E     DD+++D
Sbjct: 217 EVGSELDDSDDD 228
>sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog)
          Length = 8545

 Score = 37.7 bits (86), Expect = 0.021
 Identities = 23/67 (34%), Positives = 36/67 (53%), Gaps = 2/67 (2%)
 Frame = +1

Query: 16   NMSTVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKK--QRLSDLNDIKDSEEKAQ 189
            N  T+E +TP+ EE   L + IEKK  +   V  ++P  +   Q+L D+  I + EE   
Sbjct: 2043 NPETIETVTPILEEYTTLVDNIEKKLANEIEVPTNDPSRQDLVQQLQDV--ILECEEVVV 2100

Query: 190  NGDDAEE 210
            N D+ E+
Sbjct: 2101 NCDNIEK 2107
>sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (Upstream binding factor 1-B)
           (UBF-1-B) (xUBF-2)
          Length = 701

 Score = 35.8 bits (81), Expect = 0.079
 Identities = 25/119 (21%), Positives = 54/119 (45%)
 Frame = +1

Query: 16  NMSTVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNG 195
           N +  ++ T +    +  K VI+ K+ D E+ ++DE E       D +D +D E+ +++G
Sbjct: 589 NTNKRKSTTKIQAPSSKSKLVIQSKSDDDEDDEDDEDEED----DDDDDDEDKEDSSEDG 644

Query: 196 DDAEEDQXXXXXXXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENEVN 372
           D ++                       E   E+   + + +GSS+  + + DS++++ N
Sbjct: 645 DSSDSSSDEDSEEGEENEDEEDEEDDDEDNEED--DDDNESGSSSSSSSSADSSDSDSN 701
>sp|P36044|MNN4_YEAST Protein MNN4
          Length = 1178

 Score = 33.9 bits (76), Expect = 0.30
 Identities = 24/105 (22%), Positives = 40/105 (38%)
 Frame = +1

Query: 52   EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXX 231
            EE+   KE  EKK  + E     E E KK++  +    K+ EEK +  ++ ++ +     
Sbjct: 1047 EEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQEEEEKKKKEEEEK 1106

Query: 232  XXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 366
                            K  E+  K+K+      K  E     E+E
Sbjct: 1107 KKQEEGEKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKNEDE 1151

 Score = 30.0 bits (66), Expect = 4.3
 Identities = 25/99 (25%), Positives = 41/99 (41%)
 Frame = +1

Query: 70   KEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXX 249
            KE  EKK  + E     E E KK++  +    K+ EEK +  ++ ++ Q           
Sbjct: 1045 KEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQ----------- 1093

Query: 250  XXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 366
                     +K  EE  K+K   G   K  E+ ++ +NE
Sbjct: 1094 -----EEEEKKKKEEEEKKKQEEGEKMK-NEDEENKKNE 1126

 Score = 29.3 bits (64), Expect = 7.4
 Identities = 16/46 (34%), Positives = 26/46 (56%)
 Frame = +1

Query: 52   EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQ 189
            E+K N +E  EKK  + +N  N++ E KKQ   +    ++ E+K Q
Sbjct: 1129 EKKKNEEE--EKKKQEEKNKKNEDEEKKKQEEEEKKKNEEEEKKKQ 1172

 Score = 28.9 bits (63), Expect = 9.6
 Identities = 16/54 (29%), Positives = 29/54 (53%)
 Frame = +1

Query: 55   EKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 216
            EK   ++   KK  D E   N+E E KKQ   +  + +D E+K Q  ++ ++++
Sbjct: 1113 EKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKN-EDEEKKKQEEEEKKKNE 1165
>sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kinase CDC2L1
           (Galactosyltransferase-associated protein kinase
           p58/GTA) (Cell division cycle 2-like protein kinase 1)
          Length = 784

 Score = 33.5 bits (75), Expect = 0.39
 Identities = 22/90 (24%), Positives = 39/90 (43%)
 Frame = +1

Query: 97  DSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXXXXXXXXXXS 276
           D+     +E   ++  LSDL DI DSE K  + + +  +                    S
Sbjct: 247 DNRKPVKEEKVEERDLLSDLQDISDSERKTSSAESSSAES----------------GSGS 290

Query: 277 EKGTEEHVKEKDCNGSSAKVTENGDSAENE 366
           E+  EE  +E++  GS+++ +E  +  E E
Sbjct: 291 EEEEEEEEEEEEEEGSTSEESEEEEEEEEE 320
>sp|Q03101|CYAG_DICDI Adenylate cyclase, germination specific (ATP pyrophosphate-lyase)
           (Adenylyl cyclase)
          Length = 858

 Score = 33.5 bits (75), Expect = 0.39
 Identities = 21/76 (27%), Positives = 35/76 (46%), Gaps = 11/76 (14%)
 Frame = +1

Query: 7   NFLNMSTVENITPVTEEKNNLKEVIEKKTVDSENV-DNDEPETKKQRLSD---------- 153
           N +N++  +N+    E  NN  + IE    D+ N+ DN+   T +  +            
Sbjct: 760 NDININNSDNVNNY-ENNNNFSDKIENNDGDNNNINDNNYKSTNENNIKSKTLFKDSKSL 818

Query: 154 LNDIKDSEEKAQNGDD 201
           +NDIK ++E   N DD
Sbjct: 819 INDIKMAKENCDNNDD 834
>sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein RAD52
          Length = 505

 Score = 33.1 bits (74), Expect = 0.51
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = +1

Query: 100 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNG 195
           S  +DND P  K+Q+L++ N I D+ +   NG
Sbjct: 181 SNTIDNDNPAVKRQKLNNQNSIPDTNKVKYNG 212
>sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein
          Length = 1269

 Score = 32.7 bits (73), Expect = 0.67
 Identities = 17/55 (30%), Positives = 28/55 (50%)
 Frame = +1

Query: 52  EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 216
           ++K  LKE  E    + E+ DNDE +       +    ++ EE + + DD EED+
Sbjct: 723 DKKRKLKEEDEDDDDEEEDDDNDEGDDDDDDDDEEEGGEEGEESSDSSDDEEEDE 777
>sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor complex subunit 1
          Length = 585

 Score = 32.3 bits (72), Expect = 0.87
 Identities = 15/35 (42%), Positives = 21/35 (60%)
 Frame = +1

Query: 100 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDA 204
           S+ VD DEP++KKQRL +     D  +    GDD+
Sbjct: 361 SDQVDKDEPQSKKQRLMEKIHYVDLGDPILEGDDS 395
>sp|P46916|YTXO_BACSU Hypothetical protein ytxO (ORFY)
          Length = 143

 Score = 32.3 bits (72), Expect = 0.87
 Identities = 21/72 (29%), Positives = 38/72 (52%)
 Frame = +1

Query: 1   EVNFLNMSTVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEE 180
           E   L++ ++  +  +   K   KE  EK+T D + VD DE ETK +   +  + +DS+ 
Sbjct: 41  EEKHLDVGSIRELPHINAPKEYQKETKEKET-DRKIVD-DEEETKFETTLEPEEERDSKR 98

Query: 181 KAQNGDDAEEDQ 216
            A   ++ +ED+
Sbjct: 99  SAPPYEEEDEDE 110
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.316    0.133    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,599,100
Number of Sequences: 369166
Number of extensions: 625733
Number of successful extensions: 3961
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2845
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3778
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4259329035
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)

Cluster detail

DrC_00600

  1. Dr_sW_019_J04
  2. Dr_sW_025_I14
  3. Dr_sW_004_L18
  4. Dr_sW_002_J05
  5. Dr_sW_004_N21
  6. Dr_sW_025_B21
  7. Dr_sW_018_I17
  8. Dr_sW_008_J14
  9. Dr_sW_013_D14
  10. Dr_sW_001_I03
  11. Dr_sW_001_E15
  12. Dr_sW_002_M04
  13. Dr_sW_009_P15
  14. Dr_sW_011_E16
  15. Dr_sW_025_P05
  16. Dr_sW_013_O06
  17. Dr_sW_027_A08
  18. Dr_sW_025_N02
  19. Dr_sW_015_K24
  20. Dr_sW_028_G10