Planarian EST Database


Dr_sW_002_M04

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_002_M04
         (488 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P36044|MNN4_YEAST  Protein MNN4                                 34   0.21 
sp|P25980|UBF1B_XENLA  Nucleolar transcription factor 1-B (U...    33   0.27 
sp|P24788|CD2L1_MOUSE  PITSLRE serine/threonine-protein kina...    33   0.27 
sp|Q6FSW2|RAD52_CANGA  DNA repair and recombination protein ...    33   0.36 
sp|Q6DRL5|MBB1A_BRARE  Myb-binding protein 1A-like protein         33   0.46 
sp|Q960E8|TF2H1_DROME  TFIIH basal transcription factor comp...    32   0.61 
sp|P13816|GARP_PLAFF  Glutamic acid-rich protein precursor         32   0.61 
sp|P25977|UBF1_RAT  Nucleolar transcription factor 1 (Upstre...    32   0.79 
sp|O67124|RAD50_AQUAE  Probable DNA double-strand break repa...    32   1.0  
sp|P17480|UBF1_HUMAN  Nucleolar transcription factor 1 (Upst...    31   1.4  
>sp|P36044|MNN4_YEAST Protein MNN4
          Length = 1178

 Score = 33.9 bits (76), Expect = 0.21
 Identities = 24/105 (22%), Positives = 40/105 (38%)
 Frame = +3

Query: 6    EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXX 185
            EE+   KE  EKK  + E     E E KK++  +    K+ EEK +  ++ ++ +     
Sbjct: 1047 EEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQEEEEKKKKEEEEK 1106

Query: 186  XXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 320
                            K  E+  K+K+      K  E     E+E
Sbjct: 1107 KKQEEGEKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKNEDE 1151

 Score = 30.0 bits (66), Expect = 3.0
 Identities = 25/99 (25%), Positives = 41/99 (41%)
 Frame = +3

Query: 24   KEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXX 203
            KE  EKK  + E     E E KK++  +    K+ EEK +  ++ ++ Q           
Sbjct: 1045 KEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQ----------- 1093

Query: 204  XXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 320
                     +K  EE  K+K   G   K  E+ ++ +NE
Sbjct: 1094 -----EEEEKKKKEEEEKKKQEEGEKMK-NEDEENKKNE 1126

 Score = 29.3 bits (64), Expect = 5.1
 Identities = 16/46 (34%), Positives = 26/46 (56%)
 Frame = +3

Query: 6    EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQ 143
            E+K N +E  EKK  + +N  N++ E KKQ   +    ++ E+K Q
Sbjct: 1129 EKKKNEEE--EKKKQEEKNKKNEDEEKKKQEEEEKKKNEEEEKKKQ 1172

 Score = 28.9 bits (63), Expect = 6.7
 Identities = 16/54 (29%), Positives = 29/54 (53%)
 Frame = +3

Query: 9    EKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 170
            EK   ++   KK  D E   N+E E KKQ   +  + +D E+K Q  ++ ++++
Sbjct: 1113 EKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKN-EDEEKKKQEEEEKKKNE 1165
>sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (Upstream binding factor 1-B)
           (UBF-1-B) (xUBF-2)
          Length = 701

 Score = 33.5 bits (75), Expect = 0.27
 Identities = 23/101 (22%), Positives = 47/101 (46%)
 Frame = +3

Query: 24  KEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXX 203
           K VI+ K+ D E+ ++DE E       D +D +D E+ +++GD ++              
Sbjct: 607 KLVIQSKSDDDEDDEDDEDEED----DDDDDDEDKEDSSEDGDSSDSSSDEDSEEGEENE 662

Query: 204 XXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENEVN 326
                    E   E+   + + +GSS+  + + DS++++ N
Sbjct: 663 DEEDEEDDDEDNEED--DDDNESGSSSSSSSSADSSDSDSN 701
>sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kinase CDC2L1
           (Galactosyltransferase-associated protein kinase
           p58/GTA) (Cell division cycle 2-like protein kinase 1)
          Length = 784

 Score = 33.5 bits (75), Expect = 0.27
 Identities = 22/90 (24%), Positives = 39/90 (43%)
 Frame = +3

Query: 51  DSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXXXXXXXXXXS 230
           D+     +E   ++  LSDL DI DSE K  + + +  +                    S
Sbjct: 247 DNRKPVKEEKVEERDLLSDLQDISDSERKTSSAESSSAES----------------GSGS 290

Query: 231 EKGTEEHVKEKDCNGSSAKVTENGDSAENE 320
           E+  EE  +E++  GS+++ +E  +  E E
Sbjct: 291 EEEEEEEEEEEEEEGSTSEESEEEEEEEEE 320
>sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein RAD52
          Length = 505

 Score = 33.1 bits (74), Expect = 0.36
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = +3

Query: 54  SENVDNDEPETKKQRLSDLNDIKDSEEKAQNG 149
           S  +DND P  K+Q+L++ N I D+ +   NG
Sbjct: 181 SNTIDNDNPAVKRQKLNNQNSIPDTNKVKYNG 212
>sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein
          Length = 1269

 Score = 32.7 bits (73), Expect = 0.46
 Identities = 17/55 (30%), Positives = 28/55 (50%)
 Frame = +3

Query: 6   EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 170
           ++K  LKE  E    + E+ DNDE +       +    ++ EE + + DD EED+
Sbjct: 723 DKKRKLKEEDEDDDDEEEDDDNDEGDDDDDDDDEEEGGEEGEESSDSSDDEEEDE 777
>sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor complex subunit 1
          Length = 585

 Score = 32.3 bits (72), Expect = 0.61
 Identities = 15/35 (42%), Positives = 21/35 (60%)
 Frame = +3

Query: 54  SENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDA 158
           S+ VD DEP++KKQRL +     D  +    GDD+
Sbjct: 361 SDQVDKDEPQSKKQRLMEKIHYVDLGDPILEGDDS 395
>sp|P13816|GARP_PLAFF Glutamic acid-rich protein precursor
          Length = 678

 Score = 32.3 bits (72), Expect = 0.61
 Identities = 14/54 (25%), Positives = 32/54 (59%)
 Frame = +3

Query: 9   EKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 170
           E++  +E  E++  + E  + +E E +++   +  D +D ++  ++ DDAEED+
Sbjct: 573 EEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEDEDEEDEDDAEEDEDDAEEDE 626

 Score = 31.6 bits (70), Expect = 1.0
 Identities = 17/55 (30%), Positives = 31/55 (56%), Gaps = 1/55 (1%)
 Frame = +3

Query: 6   EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNG-DDAEED 167
           EE+   +E  E++  + E  + +E E + +   D +D ++ E+ A+   DDAEED
Sbjct: 578 EEEEEEEEEEEEEEEEEEEEEEEEEEEEDEDEEDEDDAEEDEDDAEEDEDDAEED 632

 Score = 28.5 bits (62), Expect = 8.8
 Identities = 17/54 (31%), Positives = 26/54 (48%)
 Frame = +3

Query: 6   EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEED 167
           EE+   +E  E++  D +  D D+ E  +    +  D  D+EE     DD EED
Sbjct: 591 EEEEEEEEEEEEEEEDEDEEDEDDAEEDEDDAEEDED--DAEEDDDEEDDDEED 642
>sp|P25977|UBF1_RAT Nucleolar transcription factor 1 (Upstream binding factor 1)
           (UBF-1)
          Length = 764

 Score = 32.0 bits (71), Expect = 0.79
 Identities = 19/97 (19%), Positives = 42/97 (43%)
 Frame = +3

Query: 36  EKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXXXXXX 215
           E++  D EN D+ E        S  ++ +D +E   + DD ++D+               
Sbjct: 691 EEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEDDDDDEDDDED-------------- 736

Query: 216 XXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENEVN 326
                    ++  ++ +  GSS+  + +GDS++++ N
Sbjct: 737 ---------DDEDEDNESEGSSSSSSSSGDSSDSDSN 764
>sp|O67124|RAD50_AQUAE Probable DNA double-strand break repair rad50 ATPase
          Length = 978

 Score = 31.6 bits (70), Expect = 1.0
 Identities = 14/44 (31%), Positives = 27/44 (61%)
 Frame = +3

Query: 15  NNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQN 146
           N+LKE +EK   + E +  + PE  K+R+  L +++  +EK ++
Sbjct: 535 NSLKEEMEKLRNEVEELRKEIPENLKERIKKLEELRIEKEKLEH 578
>sp|P17480|UBF1_HUMAN Nucleolar transcription factor 1 (Upstream binding factor 1)
           (UBF-1) (Autoantigen NOR-90)
          Length = 764

 Score = 31.2 bits (69), Expect = 1.4
 Identities = 21/97 (21%), Positives = 43/97 (44%), Gaps = 5/97 (5%)
 Frame = +3

Query: 51  DSENVDNDEPETKKQRLSDLNDIKDS-----EEKAQNGDDAEEDQXXXXXXXXXXXXXXX 215
           D ++ D DE E   +      D  DS     E+++++GD+ EED                
Sbjct: 684 DEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDE------------ 731

Query: 216 XXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENEVN 326
                +   ++  ++ +  GSS+  + +GDS++++ N
Sbjct: 732 ----DDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN 764
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,879,899
Number of Sequences: 369166
Number of extensions: 508057
Number of successful extensions: 3341
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2288
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3170
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2970720600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)