Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_N02
(530 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage... 38 0.017
sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (U... 36 0.066
sp|P32773|TOA1_YEAST Transcription initiation factor IIA la... 36 0.066
sp|P36044|MNN4_YEAST Protein MNN4 34 0.25
sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kina... 33 0.33
sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein ... 33 0.43
sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein 33 0.56
sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor comp... 32 0.73
sp|P13816|GARP_PLAFF Glutamic acid-rich protein precursor 32 0.73
sp|Q03101|CYAG_DICDI Adenylate cyclase, germination specifi... 32 0.73
>sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog)
Length = 8545
Score = 37.7 bits (86), Expect = 0.017
Identities = 23/67 (34%), Positives = 36/67 (53%), Gaps = 2/67 (2%)
Frame = +2
Query: 8 NMSTVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKK--QRLSDLNDIKDSEEKAQ 181
N T+E +TP+ EE L + IEKK + V ++P + Q+L D+ I + EE
Sbjct: 2043 NPETIETVTPILEEYTTLVDNIEKKLANEIEVPTNDPSRQDLVQQLQDV--ILECEEVVV 2100
Query: 182 NGDDAEE 202
N D+ E+
Sbjct: 2101 NCDNIEK 2107
>sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (Upstream binding factor 1-B)
(UBF-1-B) (xUBF-2)
Length = 701
Score = 35.8 bits (81), Expect = 0.066
Identities = 25/119 (21%), Positives = 54/119 (45%)
Frame = +2
Query: 8 NMSTVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNG 187
N + ++ T + + K VI+ K+ D E+ ++DE E D +D +D E+ +++G
Sbjct: 589 NTNKRKSTTKIQAPSSKSKLVIQSKSDDDEDDEDDEDEED----DDDDDDEDKEDSSEDG 644
Query: 188 DDAEEDQXXXXXXXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENEVN 364
D ++ E E+ + + +GSS+ + + DS++++ N
Sbjct: 645 DSSDSSSDEDSEEGEENEDEEDEEDDDEDNEED--DDDNESGSSSSSSSSADSSDSDSN 701
>sp|P32773|TOA1_YEAST Transcription initiation factor IIA large chain (TFIIA 32 kDa
subunit)
Length = 286
Score = 35.8 bits (81), Expect = 0.066
Identities = 21/68 (30%), Positives = 41/68 (60%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 NMSTVENITPVTEEKNN--LKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQ 181
N++TVENI +E+K++ +E +EK + E ++ + + KK++ S L D++E
Sbjct: 164 NITTVENIDDESEKKDDEEKEEDVEKTRKEKEQIEQVKLQAKKEKRSAL---LDTDEVGS 220
Query: 182 NGDDAEED 205
DD+++D
Sbjct: 221 ELDDSDDD 228
>sp|P36044|MNN4_YEAST Protein MNN4
Length = 1178
Score = 33.9 bits (76), Expect = 0.25
Identities = 24/105 (22%), Positives = 40/105 (38%)
Frame = +2
Query: 44 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXX 223
EE+ KE EKK + E E E KK++ + K+ EEK + ++ ++ +
Sbjct: 1047 EEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQEEEEKKKKEEEEK 1106
Query: 224 XXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 358
K E+ K+K+ K E E+E
Sbjct: 1107 KKQEEGEKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKNEDE 1151
Score = 30.0 bits (66), Expect = 3.6
Identities = 25/99 (25%), Positives = 41/99 (41%)
Frame = +2
Query: 62 KEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXX 241
KE EKK + E E E KK++ + K+ EEK + ++ ++ Q
Sbjct: 1045 KEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQ----------- 1093
Query: 242 XXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 358
+K EE K+K G K E+ ++ +NE
Sbjct: 1094 -----EEEEKKKKEEEEKKKQEEGEKMK-NEDEENKKNE 1126
Score = 29.3 bits (64), Expect = 6.2
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +2
Query: 44 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQ 181
E+K N +E EKK + +N N++ E KKQ + ++ E+K Q
Sbjct: 1129 EKKKNEEE--EKKKQEEKNKKNEDEEKKKQEEEEKKKNEEEEKKKQ 1172
Score = 28.9 bits (63), Expect = 8.1
Identities = 16/54 (29%), Positives = 29/54 (53%)
Frame = +2
Query: 47 EKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 208
EK ++ KK D E N+E E KKQ + + +D E+K Q ++ ++++
Sbjct: 1113 EKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKN-EDEEKKKQEEEEKKKNE 1165
>sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kinase CDC2L1
(Galactosyltransferase-associated protein kinase
p58/GTA) (Cell division cycle 2-like protein kinase 1)
Length = 784
Score = 33.5 bits (75), Expect = 0.33
Identities = 22/90 (24%), Positives = 39/90 (43%)
Frame = +2
Query: 89 DSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXXXXXXXXXXS 268
D+ +E ++ LSDL DI DSE K + + + + S
Sbjct: 247 DNRKPVKEEKVEERDLLSDLQDISDSERKTSSAESSSAES----------------GSGS 290
Query: 269 EKGTEEHVKEKDCNGSSAKVTENGDSAENE 358
E+ EE +E++ GS+++ +E + E E
Sbjct: 291 EEEEEEEEEEEEEEGSTSEESEEEEEEEEE 320
>sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein RAD52
Length = 505
Score = 33.1 bits (74), Expect = 0.43
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +2
Query: 92 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNG 187
S +DND P K+Q+L++ N I D+ + NG
Sbjct: 181 SNTIDNDNPAVKRQKLNNQNSIPDTNKVKYNG 212
>sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein
Length = 1269
Score = 32.7 bits (73), Expect = 0.56
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +2
Query: 44 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 208
++K LKE E + E+ DNDE + + ++ EE + + DD EED+
Sbjct: 723 DKKRKLKEEDEDDDDEEEDDDNDEGDDDDDDDDEEEGGEEGEESSDSSDDEEEDE 777
>sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor complex subunit 1
Length = 585
Score = 32.3 bits (72), Expect = 0.73
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = +2
Query: 92 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDA 196
S+ VD DEP++KKQRL + D + GDD+
Sbjct: 361 SDQVDKDEPQSKKQRLMEKIHYVDLGDPILEGDDS 395
>sp|P13816|GARP_PLAFF Glutamic acid-rich protein precursor
Length = 678
Score = 32.3 bits (72), Expect = 0.73
Identities = 14/54 (25%), Positives = 32/54 (59%)
Frame = +2
Query: 47 EKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 208
E++ +E E++ + E + +E E +++ + D +D ++ ++ DDAEED+
Sbjct: 573 EEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEDEDEEDEDDAEEDEDDAEEDE 626
Score = 31.6 bits (70), Expect = 1.3
Identities = 17/55 (30%), Positives = 31/55 (56%), Gaps = 1/55 (1%)
Frame = +2
Query: 44 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNG-DDAEED 205
EE+ +E E++ + E + +E E + + D +D ++ E+ A+ DDAEED
Sbjct: 578 EEEEEEEEEEEEEEEEEEEEEEEEEEEEDEDEEDEDDAEEDEDDAEEDEDDAEED 632
>sp|Q03101|CYAG_DICDI Adenylate cyclase, germination specific (ATP pyrophosphate-lyase)
(Adenylyl cyclase)
Length = 858
Score = 32.3 bits (72), Expect = 0.73
Identities = 20/74 (27%), Positives = 34/74 (45%), Gaps = 11/74 (14%)
Frame = +2
Query: 5 LNMSTVENITPVTEEKNNLKEVIEKKTVDSENV-DNDEPETKKQRLSD----------LN 151
+N++ +N+ E NN + IE D+ N+ DN+ T + + +N
Sbjct: 762 ININNSDNVNNY-ENNNNFSDKIENNDGDNNNINDNNYKSTNENNIKSKTLFKDSKSLIN 820
Query: 152 DIKDSEEKAQNGDD 193
DIK ++E N DD
Sbjct: 821 DIKMAKENCDNNDD 834
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,174,919
Number of Sequences: 369166
Number of extensions: 567275
Number of successful extensions: 3797
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2695
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3616
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3600891075
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)