Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_E16
(500 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (U... 35 0.13
sp|P36044|MNN4_YEAST Protein MNN4 34 0.22
sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kina... 33 0.29
sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein ... 33 0.38
sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein 33 0.49
sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor comp... 32 0.65
sp|P13816|GARP_PLAFF Glutamic acid-rich protein precursor 32 0.65
sp|P25977|UBF1_RAT Nucleolar transcription factor 1 (Upstre... 32 0.84
sp|P52194|CLGN_MOUSE Calmegin precursor (MEG 1 antigen) (Ca... 32 1.1
sp|O67124|RAD50_AQUAE Probable DNA double-strand break repa... 32 1.1
>sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (Upstream binding factor 1-B)
(UBF-1-B) (xUBF-2)
Length = 701
Score = 34.7 bits (78), Expect = 0.13
Identities = 24/111 (21%), Positives = 50/111 (45%)
Frame = +3
Query: 6 TPVTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQX 185
T + + K VI+ K+ D E+ ++DE E D +D +D E+ +++GD ++
Sbjct: 597 TKIQAPSSKSKLVIQSKSDDDEDDEDDEDEED----DDDDDDEDKEDSSEDGDSSDSSSD 652
Query: 186 XXXXXXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENEVN 338
E E+ + + +GSS+ + + DS++++ N
Sbjct: 653 EDSEEGEENEDEEDEEDDDEDNEED--DDDNESGSSSSSSSSADSSDSDSN 701
>sp|P36044|MNN4_YEAST Protein MNN4
Length = 1178
Score = 33.9 bits (76), Expect = 0.22
Identities = 24/105 (22%), Positives = 40/105 (38%)
Frame = +3
Query: 18 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXX 197
EE+ KE EKK + E E E KK++ + K+ EEK + ++ ++ +
Sbjct: 1047 EEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQEEEEKKKKEEEEK 1106
Query: 198 XXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 332
K E+ K+K+ K E E+E
Sbjct: 1107 KKQEEGEKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKNEDE 1151
Score = 30.0 bits (66), Expect = 3.2
Identities = 25/99 (25%), Positives = 41/99 (41%)
Frame = +3
Query: 36 KEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXX 215
KE EKK + E E E KK++ + K+ EEK + ++ ++ Q
Sbjct: 1045 KEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQ----------- 1093
Query: 216 XXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 332
+K EE K+K G K E+ ++ +NE
Sbjct: 1094 -----EEEEKKKKEEEEKKKQEEGEKMK-NEDEENKKNE 1126
Score = 29.3 bits (64), Expect = 5.5
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +3
Query: 18 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQ 155
E+K N +E EKK + +N N++ E KKQ + ++ E+K Q
Sbjct: 1129 EKKKNEEE--EKKKQEEKNKKNEDEEKKKQEEEEKKKNEEEEKKKQ 1172
Score = 28.9 bits (63), Expect = 7.1
Identities = 16/54 (29%), Positives = 29/54 (53%)
Frame = +3
Query: 21 EKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 182
EK ++ KK D E N+E E KKQ + + +D E+K Q ++ ++++
Sbjct: 1113 EKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKN-EDEEKKKQEEEEKKKNE 1165
>sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kinase CDC2L1
(Galactosyltransferase-associated protein kinase
p58/GTA) (Cell division cycle 2-like protein kinase 1)
Length = 784
Score = 33.5 bits (75), Expect = 0.29
Identities = 22/90 (24%), Positives = 39/90 (43%)
Frame = +3
Query: 63 DSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXXXXXXXXXXS 242
D+ +E ++ LSDL DI DSE K + + + + S
Sbjct: 247 DNRKPVKEEKVEERDLLSDLQDISDSERKTSSAESSSAES----------------GSGS 290
Query: 243 EKGTEEHVKEKDCNGSSAKVTENGDSAENE 332
E+ EE +E++ GS+++ +E + E E
Sbjct: 291 EEEEEEEEEEEEEEGSTSEESEEEEEEEEE 320
>sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein RAD52
Length = 505
Score = 33.1 bits (74), Expect = 0.38
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +3
Query: 66 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNG 161
S +DND P K+Q+L++ N I D+ + NG
Sbjct: 181 SNTIDNDNPAVKRQKLNNQNSIPDTNKVKYNG 212
>sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein
Length = 1269
Score = 32.7 bits (73), Expect = 0.49
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +3
Query: 18 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 182
++K LKE E + E+ DNDE + + ++ EE + + DD EED+
Sbjct: 723 DKKRKLKEEDEDDDDEEEDDDNDEGDDDDDDDDEEEGGEEGEESSDSSDDEEEDE 777
>sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor complex subunit 1
Length = 585
Score = 32.3 bits (72), Expect = 0.65
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = +3
Query: 66 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDA 170
S+ VD DEP++KKQRL + D + GDD+
Sbjct: 361 SDQVDKDEPQSKKQRLMEKIHYVDLGDPILEGDDS 395
>sp|P13816|GARP_PLAFF Glutamic acid-rich protein precursor
Length = 678
Score = 32.3 bits (72), Expect = 0.65
Identities = 14/54 (25%), Positives = 32/54 (59%)
Frame = +3
Query: 21 EKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQ 182
E++ +E E++ + E + +E E +++ + D +D ++ ++ DDAEED+
Sbjct: 573 EEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEDEDEEDEDDAEEDEDDAEEDE 626
Score = 31.6 bits (70), Expect = 1.1
Identities = 17/55 (30%), Positives = 31/55 (56%), Gaps = 1/55 (1%)
Frame = +3
Query: 18 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNG-DDAEED 179
EE+ +E E++ + E + +E E + + D +D ++ E+ A+ DDAEED
Sbjct: 578 EEEEEEEEEEEEEEEEEEEEEEEEEEEEDEDEEDEDDAEEDEDDAEEDEDDAEED 632
Score = 28.5 bits (62), Expect = 9.3
Identities = 17/54 (31%), Positives = 26/54 (48%)
Frame = +3
Query: 18 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEED 179
EE+ +E E++ D + D D+ E + + D D+EE DD EED
Sbjct: 591 EEEEEEEEEEEEEEEDEDEEDEDDAEEDEDDAEEDED--DAEEDDDEEDDDEED 642
>sp|P25977|UBF1_RAT Nucleolar transcription factor 1 (Upstream binding factor 1)
(UBF-1)
Length = 764
Score = 32.0 bits (71), Expect = 0.84
Identities = 19/97 (19%), Positives = 42/97 (43%)
Frame = +3
Query: 48 EKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEDQXXXXXXXXXXXXXXX 227
E++ D EN D+ E S ++ +D +E + DD ++D+
Sbjct: 691 EEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEDDDDDEDDDED-------------- 736
Query: 228 XXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENEVN 338
++ ++ + GSS+ + +GDS++++ N
Sbjct: 737 ---------DDEDEDNESEGSSSSSSSSGDSSDSDSN 764
>sp|P52194|CLGN_MOUSE Calmegin precursor (MEG 1 antigen) (Calnexin-T) (A2/6)
Length = 611
Score = 31.6 bits (70), Expect = 1.1
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = +3
Query: 12 VTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGD 164
VT EK + E EK D E ++ E +K + +++KD++E +GD
Sbjct: 547 VTVEKEVIGEPEEKSKEDRETLEGQEEVSKLSKSGSEDEMKDADESPGSGD 597
>sp|O67124|RAD50_AQUAE Probable DNA double-strand break repair rad50 ATPase
Length = 978
Score = 31.6 bits (70), Expect = 1.1
Identities = 14/44 (31%), Positives = 27/44 (61%)
Frame = +3
Query: 27 NNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQN 158
N+LKE +EK + E + + PE K+R+ L +++ +EK ++
Sbjct: 535 NSLKEEMEKLRNEVEELRKEIPENLKERIKKLEELRIEKEKLEH 578
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,421,414
Number of Sequences: 369166
Number of extensions: 526511
Number of successful extensions: 3492
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2419
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3321
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3168768640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)