Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_J05
(555 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage... 39 0.009
sp|P32773|TOA1_YEAST Transcription initiation factor IIA la... 38 0.019
sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (U... 35 0.12
sp|P36044|MNN4_YEAST Protein MNN4 33 0.36
sp|Q03101|CYAG_DICDI Adenylate cyclase, germination specifi... 33 0.36
sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein 33 0.47
sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein ... 33 0.47
sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor comp... 32 0.80
sp|P11075|SEC7_YEAST Protein transport protein SEC7 32 0.80
sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kina... 32 1.0
>sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog)
Length = 8545
Score = 38.9 bits (89), Expect = 0.009
Identities = 23/69 (33%), Positives = 38/69 (55%), Gaps = 2/69 (2%)
Frame = +1
Query: 37 NMSTVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKK--QRLSDLNDIKDSEEKAQ 210
N T+E +TP+ EE L + IEKK + V ++P + Q+L D+ I + EE
Sbjct: 2043 NPETIETVTPILEEYTTLVDNIEKKLANEIEVPTNDPSRQDLVQQLQDV--ILECEEVVV 2100
Query: 211 NGDDAEEVQ 237
N D+ E+++
Sbjct: 2101 NCDNIEKLE 2109
>sp|P32773|TOA1_YEAST Transcription initiation factor IIA large chain (TFIIA 32 kDa
subunit)
Length = 286
Score = 37.7 bits (86), Expect = 0.019
Identities = 21/70 (30%), Positives = 40/70 (57%), Gaps = 2/70 (2%)
Frame = +1
Query: 19 LKVNFLNMSTVENITPVTEEKNN--LKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKD 192
L +N N++TVENI +E+K++ +E +EK + E ++ + + KK++ S L D +
Sbjct: 158 LTINNANITTVENIDDESEKKDDEEKEEDVEKTRKEKEQIEQVKLQAKKEKRSALLDTDE 217
Query: 193 SEEKAQNGDD 222
+ + DD
Sbjct: 218 VGSELDDSDD 227
>sp|P25980|UBF1B_XENLA Nucleolar transcription factor 1-B (Upstream binding factor 1-B)
(UBF-1-B) (xUBF-2)
Length = 701
Score = 35.0 bits (79), Expect = 0.12
Identities = 25/119 (21%), Positives = 54/119 (45%)
Frame = +1
Query: 37 NMSTVENITPVTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNG 216
N + ++ T + + K VI+ K+ D E+ ++DE E D +D +D E+ +++G
Sbjct: 589 NTNKRKSTTKIQAPSSKSKLVIQSKSDDDEDDEDDEDEED----DDDDDDEDKEDSSEDG 644
Query: 217 DDAEEVQXXXXXXXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENEVN 393
D ++ E E+ + + +GSS+ + + DS++++ N
Sbjct: 645 DSSDSSSDEDSEEGEENEDEEDEEDDDEDNEED--DDDNESGSSSSSSSSADSSDSDSN 701
>sp|P36044|MNN4_YEAST Protein MNN4
Length = 1178
Score = 33.5 bits (75), Expect = 0.36
Identities = 24/105 (22%), Positives = 40/105 (38%)
Frame = +1
Query: 73 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEVQXXXXX 252
EE+ KE EKK + E E E KK++ + K+ EEK + ++ ++ +
Sbjct: 1047 EEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQEEEEKKKKEEEEK 1106
Query: 253 XXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 387
K E+ K+K+ K E E+E
Sbjct: 1107 KKQEEGEKMKNEDEENKKNEDEEKKKNEEEEKKKQEEKNKKNEDE 1151
Score = 29.6 bits (65), Expect = 5.2
Identities = 25/99 (25%), Positives = 41/99 (41%)
Frame = +1
Query: 91 KEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEVQXXXXXXXXXXX 270
KE EKK + E E E KK++ + K+ EEK + ++ ++ Q
Sbjct: 1045 KEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKKEEEEKKKQ----------- 1093
Query: 271 XXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 387
+K EE K+K G K E+ ++ +NE
Sbjct: 1094 -----EEEEKKKKEEEEKKKQEEGEKMK-NEDEENKKNE 1126
Score = 29.3 bits (64), Expect = 6.8
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +1
Query: 73 EEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDIKDSEEKAQ 210
E+K N +E EKK + +N N++ E KKQ + ++ E+K Q
Sbjct: 1129 EKKKNEEE--EKKKQEEKNKKNEDEEKKKQEEEEKKKNEEEEKKKQ 1172
>sp|Q03101|CYAG_DICDI Adenylate cyclase, germination specific (ATP pyrophosphate-lyase)
(Adenylyl cyclase)
Length = 858
Score = 33.5 bits (75), Expect = 0.36
Identities = 21/76 (27%), Positives = 35/76 (46%), Gaps = 11/76 (14%)
Frame = +1
Query: 28 NFLNMSTVENITPVTEEKNNLKEVIEKKTVDSENV-DNDEPETKKQRLSD---------- 174
N +N++ +N+ E NN + IE D+ N+ DN+ T + +
Sbjct: 760 NDININNSDNVNNY-ENNNNFSDKIENNDGDNNNINDNNYKSTNENNIKSKTLFKDSKSL 818
Query: 175 LNDIKDSEEKAQNGDD 222
+NDIK ++E N DD
Sbjct: 819 INDIKMAKENCDNNDD 834
>sp|Q6DRL5|MBB1A_BRARE Myb-binding protein 1A-like protein
Length = 1269
Score = 33.1 bits (74), Expect = 0.47
Identities = 25/76 (32%), Positives = 36/76 (47%), Gaps = 1/76 (1%)
Frame = +1
Query: 7 LHSILKVNFLNMSTVENITPVTEEKNN-LKEVIEKKTVDSENVDNDEPETKKQRLSDLND 183
L SIL V N E+ VT++K LKE E + E+ DNDE + +
Sbjct: 700 LSSILNVLDPNKDEDESGVVVTDDKKRKLKEEDEDDDDEEEDDDNDEGDDDDDDDDEEEG 759
Query: 184 IKDSEEKAQNGDDAEE 231
++ EE + + DD EE
Sbjct: 760 GEEGEESSDSSDDEEE 775
>sp|Q6FSW2|RAD52_CANGA DNA repair and recombination protein RAD52
Length = 505
Score = 33.1 bits (74), Expect = 0.47
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +1
Query: 121 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNG 216
S +DND P K+Q+L++ N I D+ + NG
Sbjct: 181 SNTIDNDNPAVKRQKLNNQNSIPDTNKVKYNG 212
>sp|Q960E8|TF2H1_DROME TFIIH basal transcription factor complex subunit 1
Length = 585
Score = 32.3 bits (72), Expect = 0.80
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = +1
Query: 121 SENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDA 225
S+ VD DEP++KKQRL + D + GDD+
Sbjct: 361 SDQVDKDEPQSKKQRLMEKIHYVDLGDPILEGDDS 395
>sp|P11075|SEC7_YEAST Protein transport protein SEC7
Length = 2009
Score = 32.3 bits (72), Expect = 0.80
Identities = 28/111 (25%), Positives = 42/111 (37%), Gaps = 2/111 (1%)
Frame = +1
Query: 61 TPVTEEKNNLKEVIEKKTVDSENVDNDEPETKKQRLSDLNDI--KDSEEKAQNGDDAEEV 234
TP E N K ++ E+ D DE E + D D+ SE +++G+D+E V
Sbjct: 76 TPEETEDTNDKRHDDEGEDGDEDEDEDEDEDEDNGDEDDEDVDSSSSETSSEDGEDSESV 135
Query: 235 QXXXXXXXXXXXXXXXXXXXSEKGTEEHVKEKDCNGSSAKVTENGDSAENE 387
S G +E E D N S++ + S E E
Sbjct: 136 S--------------GESTESSSGEDEESDESDGNTSNSSSGDESGSEEEE 172
>sp|P24788|CD2L1_MOUSE PITSLRE serine/threonine-protein kinase CDC2L1
(Galactosyltransferase-associated protein kinase
p58/GTA) (Cell division cycle 2-like protein kinase 1)
Length = 784
Score = 32.0 bits (71), Expect = 1.0
Identities = 22/90 (24%), Positives = 38/90 (42%)
Frame = +1
Query: 118 DSENVDNDEPETKKQRLSDLNDIKDSEEKAQNGDDAEEVQXXXXXXXXXXXXXXXXXXXS 297
D+ +E ++ LSDL DI DSE K + + + S
Sbjct: 247 DNRKPVKEEKVEERDLLSDLQDISDSERKTSSAESSS----------------AESGSGS 290
Query: 298 EKGTEEHVKEKDCNGSSAKVTENGDSAENE 387
E+ EE +E++ GS+++ +E + E E
Sbjct: 291 EEEEEEEEEEEEEEGSTSEESEEEEEEEEE 320
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.317 0.133 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,450,433
Number of Sequences: 369166
Number of extensions: 605976
Number of successful extensions: 2897
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2649
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2871
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3931403200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)