Planaria EST Database

DrC_01007

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01007
         (1333 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q00277|GPX1_SCHMA  Glutathione peroxidase (GPX)                206   2e-52
sp|Q9N2J2|GPX4_BOVIN  Phospholipid hydroperoxide glutathione...   176   2e-43
sp|Q4AEH2|GPX4_PONPY  Phospholipid hydroperoxide glutathione...   175   3e-43
sp|O24031|GPX4_LYCES  Probable phospholipid hydroperoxide gl...   174   4e-43
sp|P36968|GPX4_PIG  Phospholipid hydroperoxide glutathione p...   174   5e-43
sp|Q4AEG9|GPX4_CEBAP  Phospholipid hydroperoxide glutathione...   174   7e-43
sp|Q4AEH0|GPX4_MACFU  Phospholipid hydroperoxide glutathione...   172   2e-42
sp|P30708|GPX4_NICSY  Probable phospholipid hydroperoxide gl...   172   3e-42
sp|Q4AEH1|GPX4_HYLLA  Phospholipid hydroperoxide glutathione...   171   3e-42
sp|Q91XR9|GPX42_MOUSE  Phospholipid hydroperoxide glutathion...   171   3e-42

>sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX)
          Length = 169

 Score =  206 bits (523), Expect = 2e-52
 Identities = 99/164 (60%), Positives = 125/164 (76%)
 Frame = +2

Query: 404 KNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGL 583
           K+  SIY+F+ K I+G ++SL KY+G+V LIVNVACK GAT KNY QLQE+H  L  KGL
Sbjct: 6   KSWNSIYEFTVKDINGVDVSLEKYRGHVCLIVNVACKCGATDKNYRQLQEMHTRLVGKGL 65

Query: 584 RILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGF 763
           RIL FPCNQFGGQEPWAE EIK++V + +  +FD+FSKI+VNG +A  L+K+LK +  G 
Sbjct: 66  RILAFPCNQFGGQEPWAEAEIKKFVTEKYGVQFDMFSKIKVNGSDADDLYKFLKSRQHGT 125

Query: 764 LGDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 895
           L + IKWNF+KFL+DR G PVKRY PT++P  D + DI   LEK
Sbjct: 126 LTNNIKWNFSKFLVDRQGQPVKRYSPTTAPY-DIEGDIMELLEK 168

>sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
           precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
          Length = 197

 Score =  176 bits (445), Expect = 2e-43
 Identities = 89/159 (55%), Positives = 116/159 (72%), Gaps = 2/159 (1%)
 Frame = +2

Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
           AS D  + A S+++FSAK IDG  ++L KY+G+V ++ NVA + G T  NY QL +LH  
Sbjct: 30  ASRDDWRCARSMHEFSAKDIDGRMVNLDKYRGHVCIVTNVASQCGKTDVNYTQLVDLHAR 89

Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 745
            A+ GLRIL FPCNQFG QEP +  EIKE+    +N +FDLFSKI VNGD+AHPL+K++K
Sbjct: 90  YAECGLRILAFPCNQFGRQEPGSNAEIKEFA-AGYNVKFDLFSKICVNGDDAHPLWKWMK 148

Query: 746 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 856
           +  K +G LG+ IKWNFTKFLID++G  VKRYGP   P+
Sbjct: 149 VQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187

>sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
           precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
 sp|P36969|GPX4_HUMAN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
           precursor (PHGPx) (GPX-4)
          Length = 197

 Score =  175 bits (443), Expect = 3e-43
 Identities = 89/159 (55%), Positives = 115/159 (72%), Gaps = 2/159 (1%)
 Frame = +2

Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
           AS D  + A S+++FSAK IDG  ++L KY+G V ++ NVA + G T  NY QL +LH  
Sbjct: 30  ASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89

Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 745
            A+ GLRIL FPCNQFG QEP +  EIKE+    +N +FD+FSKI VNGD+AHPL+K++K
Sbjct: 90  YAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

Query: 746 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 856
           +  K KG LG+ IKWNFTKFLID++G  VKRYGP   P+
Sbjct: 149 IQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187

>sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
          Length = 169

 Score =  174 bits (442), Expect = 4e-43
 Identities = 84/163 (51%), Positives = 112/163 (68%)
 Frame = +2

Query: 407 NATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLR 586
           N  S+YDF+ K   G+++ LS YKG V++IVNVA + G T  NY  + EL+K   D+GL 
Sbjct: 7   NPQSVYDFTVKDAKGKDVDLSIYKGKVLIIVNVASQCGLTNSNYTDMTELYKKYKDQGLE 66

Query: 587 ILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFL 766
           IL FPCNQFGGQEP    +I++ V   F AE+ +F K++VNGDNA PL+++LK    GF 
Sbjct: 67  ILAFPCNQFGGQEPGNIEDIQQMVCTRFKAEYPIFDKVDVNGDNAAPLYRFLKSSKGGFF 126

Query: 767 GDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 895
           GDGIKWNF+KFLID+ G  V RY PT+SP     A +E +++K
Sbjct: 127 GDGIKWNFSKFLIDKEGHVVDRYSPTTSP-----ASMEKDIKK 164

>sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
           precursor (PHGPx) (GPX-4)
          Length = 197

 Score =  174 bits (441), Expect = 5e-43
 Identities = 88/158 (55%), Positives = 115/158 (72%), Gaps = 2/158 (1%)
 Frame = +2

Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
           AS D  + A S+++FSAK IDG  ++L KY+G V ++ NVA + G T  NY QL +LH  
Sbjct: 30  ASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGYVCIVTNVASQCGKTEVNYTQLVDLHAR 89

Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 745
            A+ GLRIL FPCNQFG QEP ++ EIKE+    +N +FD+FSKI VNGD+AHPL+K++K
Sbjct: 90  YAECGLRILAFPCNQFGRQEPGSDAEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

Query: 746 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSP 853
           +  K +G LG+ IKWNFTKFLID++G  VKRYGP   P
Sbjct: 149 VQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEP 186

>sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
           precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
          Length = 197

 Score =  174 bits (440), Expect = 7e-43
 Identities = 89/158 (56%), Positives = 114/158 (72%), Gaps = 2/158 (1%)
 Frame = +2

Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
           AS D  + A S+++FSAK IDG  ++L KY+G V ++ NVA + G T  NY QL +LH  
Sbjct: 30  ASRDDWRCAGSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89

Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 745
            A+ GLRIL FPCNQFG QEP +  EIKE+    +N +FD+FSKI VNGD+AHPL+K++K
Sbjct: 90  YAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

Query: 746 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSP 853
           +  K KG LG+ IKWNFTKFLID++G  VKRYGP   P
Sbjct: 149 IQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEP 186

>sp|Q4AEH0|GPX4_MACFU Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
           precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
          Length = 197

 Score =  172 bits (436), Expect = 2e-42
 Identities = 87/159 (54%), Positives = 115/159 (72%), Gaps = 2/159 (1%)
 Frame = +2

Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
           AS D  + A S+++FSAK IDG  ++L KY+G V ++ NVA + G T  NY QL +LH  
Sbjct: 30  ASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89

Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 745
            A+ G+RIL FPCNQFG QEP +  +IKE+    +N +FD+FSKI VNGD+AHPL+K++K
Sbjct: 90  YAECGVRILAFPCNQFGKQEPGSNEKIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

Query: 746 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 856
           +  K KG LG+ IKWNFTKFLID++G  VKRYGP   P+
Sbjct: 149 IQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187

>sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
           (6P229)
          Length = 169

 Score =  172 bits (435), Expect = 3e-42
 Identities = 83/160 (51%), Positives = 109/160 (68%)
 Frame = +2

Query: 416 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 595
           SIYDF+ K   G ++ LS YKG V++IVNVA + G T  NY  L E++K   D+GL IL 
Sbjct: 10  SIYDFTVKDAKGNDVDLSIYKGKVLIIVNVASQCGLTNSNYTDLTEIYKKYKDQGLEILA 69

Query: 596 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDG 775
           FPCNQFGGQEP +  EI+  V   F AE+ +F K++VNGDNA PL+K+LK    GF GD 
Sbjct: 70  FPCNQFGGQEPGSIEEIQNMVCTRFKAEYPIFDKVDVNGDNAAPLYKFLKSSKGGFFGDS 129

Query: 776 IKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 895
           IKWNF+KFL+D+ G  V RY PT++P     A +E +++K
Sbjct: 130 IKWNFSKFLVDKEGNVVDRYSPTTTP-----ASMEKDIKK 164

>sp|Q4AEH1|GPX4_HYLLA Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
           precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
          Length = 197

 Score =  171 bits (434), Expect = 3e-42
 Identities = 86/159 (54%), Positives = 113/159 (71%), Gaps = 2/159 (1%)
 Frame = +2

Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
           AS D  + A S+++FSAK +DG  ++L KY+G V ++ NVA + G T  NY QL +LH  
Sbjct: 30  ASRDDWRCAGSMHEFSAKVLDGHTVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89

Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 745
            A+ GLR L FPCNQFG QEP +  EIKE+    +N +FD+FSKI VNGD+AHPL+K++K
Sbjct: 90  YAECGLRFLAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148

Query: 746 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 856
           +  K KG LG+ IKWNFTKFL D++G  VKRYGP   P+
Sbjct: 149 IQPKGKGILGNAIKWNFTKFLFDKNGCVVKRYGPMEEPL 187

>sp|Q91XR9|GPX42_MOUSE Phospholipid hydroperoxide glutathione peroxidase, nuclear (GPX-4)
          Length = 253

 Score =  171 bits (434), Expect = 3e-42
 Identities = 88/161 (54%), Positives = 114/161 (70%), Gaps = 2/161 (1%)
 Frame = +2

Query: 377 G*MASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQEL 556
           G  AS D  + A S+++FSAK IDG  + L KY+G V ++ NVA + G T  NY QL +L
Sbjct: 83  GLCASRDDWRCARSMHEFSAKDIDGHMVCLDKYRGFVCIVTNVASQCGKTDVNYTQLVDL 142

Query: 557 HKLLADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFK 736
           H   A+ GLRIL FPCNQFG QEP +  EIKE+    +N +FD++SKI VNGD+AHPL+K
Sbjct: 143 HARYAECGLRILAFPCNQFGRQEPGSNQEIKEFA-AGYNVKFDMYSKICVNGDDAHPLWK 201

Query: 737 YLKM--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSP 853
           ++K+  K +G LG+ IKWNFTKFLID++G  VKRYGP   P
Sbjct: 202 WMKVQPKGRGMLGNAIKWNFTKFLIDKNGCEVKRYGPMEEP 242

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 146,249,108
Number of Sequences: 369166
Number of extensions: 3019047
Number of successful extensions: 7601
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6971
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7421
length of database: 68,354,980
effective HSP length: 114
effective length of database: 47,295,190
effective search space used: 15560117510
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01007

  1. Dr_sW_025_H10
  2. Dr_sW_002_P22
  3. Dr_sW_005_H05
  4. Dr_sW_009_O15
  5. Dr_sW_011_H13
  6. Dr_sW_008_B01
  7. Dr_sW_001_M22
  8. Dr_sW_007_P14
  9. Dr_sW_006_K17
  10. Dr_sW_011_I14
  11. Dr_sW_015_A05