Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_H13
(768 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX) 206 7e-53
sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione... 176 8e-44
sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione... 175 1e-43
sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione p... 175 1e-43
sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide gl... 174 2e-43
sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione... 174 3e-43
sp|Q4AEH0|GPX4_MACFU Phospholipid hydroperoxide glutathione... 172 9e-43
sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide gl... 172 1e-42
sp|Q4AEH1|GPX4_HYLLA Phospholipid hydroperoxide glutathione... 171 2e-42
sp|Q91XR9|GPX42_MOUSE Phospholipid hydroperoxide glutathion... 171 2e-42
>sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX)
Length = 169
Score = 206 bits (523), Expect = 7e-53
Identities = 99/164 (60%), Positives = 125/164 (76%)
Frame = +1
Query: 58 KNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGL 237
K+ SIY+F+ K I+G ++SL KY+G+V LIVNVACK GAT KNY QLQE+H L KGL
Sbjct: 6 KSWNSIYEFTVKDINGVDVSLEKYRGHVCLIVNVACKCGATDKNYRQLQEMHTRLVGKGL 65
Query: 238 RILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGF 417
RIL FPCNQFGGQEPWAE EIK++V + + +FD+FSKI+VNG +A L+K+LK + G
Sbjct: 66 RILAFPCNQFGGQEPWAEAEIKKFVTEKYGVQFDMFSKIKVNGSDADDLYKFLKSRQHGT 125
Query: 418 LGDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 549
L + IKWNF+KFL+DR G PVKRY PT++P D + DI LEK
Sbjct: 126 LTNNIKWNFSKFLVDRQGQPVKRYSPTTAPY-DIEGDIMELLEK 168
>sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 176 bits (445), Expect = 8e-44
Identities = 89/159 (55%), Positives = 116/159 (72%), Gaps = 2/159 (1%)
Frame = +1
Query: 40 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 219
AS D + A S+++FSAK IDG ++L KY+G+V ++ NVA + G T NY QL +LH
Sbjct: 30 ASRDDWRCARSMHEFSAKDIDGRMVNLDKYRGHVCIVTNVASQCGKTDVNYTQLVDLHAR 89
Query: 220 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 399
A+ GLRIL FPCNQFG QEP + EIKE+ +N +FDLFSKI VNGD+AHPL+K++K
Sbjct: 90 YAECGLRILAFPCNQFGRQEPGSNAEIKEFA-AGYNVKFDLFSKICVNGDDAHPLWKWMK 148
Query: 400 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 510
+ K +G LG+ IKWNFTKFLID++G VKRYGP P+
Sbjct: 149 VQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
>sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
sp|P36969|GPX4_HUMAN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 175 bits (443), Expect = 1e-43
Identities = 89/159 (55%), Positives = 115/159 (72%), Gaps = 2/159 (1%)
Frame = +1
Query: 40 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 219
AS D + A S+++FSAK IDG ++L KY+G V ++ NVA + G T NY QL +LH
Sbjct: 30 ASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89
Query: 220 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 399
A+ GLRIL FPCNQFG QEP + EIKE+ +N +FD+FSKI VNGD+AHPL+K++K
Sbjct: 90 YAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148
Query: 400 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 510
+ K KG LG+ IKWNFTKFLID++G VKRYGP P+
Sbjct: 149 IQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
>sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 175 bits (443), Expect = 1e-43
Identities = 89/169 (52%), Positives = 120/169 (71%), Gaps = 2/169 (1%)
Frame = +1
Query: 7 LPIQSVSLSKMASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAK 186
L + ++ + AS D + A S+++FSAK IDG ++L KY+G V ++ NVA + G T
Sbjct: 19 LAVPGLAGTMCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGYVCIVTNVASQCGKTEV 78
Query: 187 NYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNG 366
NY QL +LH A+ GLRIL FPCNQFG QEP ++ EIKE+ +N +FD+FSKI VNG
Sbjct: 79 NYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSDAEIKEFA-AGYNVKFDMFSKICVNG 137
Query: 367 DNAHPLFKYLKM--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSP 507
D+AHPL+K++K+ K +G LG+ IKWNFTKFLID++G VKRYGP P
Sbjct: 138 DDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEP 186
>sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Length = 169
Score = 174 bits (442), Expect = 2e-43
Identities = 84/163 (51%), Positives = 112/163 (68%)
Frame = +1
Query: 61 NATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLR 240
N S+YDF+ K G+++ LS YKG V++IVNVA + G T NY + EL+K D+GL
Sbjct: 7 NPQSVYDFTVKDAKGKDVDLSIYKGKVLIIVNVASQCGLTNSNYTDMTELYKKYKDQGLE 66
Query: 241 ILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFL 420
IL FPCNQFGGQEP +I++ V F AE+ +F K++VNGDNA PL+++LK GF
Sbjct: 67 ILAFPCNQFGGQEPGNIEDIQQMVCTRFKAEYPIFDKVDVNGDNAAPLYRFLKSSKGGFF 126
Query: 421 GDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 549
GDGIKWNF+KFLID+ G V RY PT+SP A +E +++K
Sbjct: 127 GDGIKWNFSKFLIDKEGHVVDRYSPTTSP-----ASMEKDIKK 164
>sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 174 bits (440), Expect = 3e-43
Identities = 89/158 (56%), Positives = 114/158 (72%), Gaps = 2/158 (1%)
Frame = +1
Query: 40 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 219
AS D + A S+++FSAK IDG ++L KY+G V ++ NVA + G T NY QL +LH
Sbjct: 30 ASRDDWRCAGSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89
Query: 220 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 399
A+ GLRIL FPCNQFG QEP + EIKE+ +N +FD+FSKI VNGD+AHPL+K++K
Sbjct: 90 YAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148
Query: 400 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSP 507
+ K KG LG+ IKWNFTKFLID++G VKRYGP P
Sbjct: 149 IQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEP 186
>sp|Q4AEH0|GPX4_MACFU Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 172 bits (436), Expect = 9e-43
Identities = 87/159 (54%), Positives = 115/159 (72%), Gaps = 2/159 (1%)
Frame = +1
Query: 40 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 219
AS D + A S+++FSAK IDG ++L KY+G V ++ NVA + G T NY QL +LH
Sbjct: 30 ASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89
Query: 220 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 399
A+ G+RIL FPCNQFG QEP + +IKE+ +N +FD+FSKI VNGD+AHPL+K++K
Sbjct: 90 YAECGVRILAFPCNQFGKQEPGSNEKIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148
Query: 400 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 510
+ K KG LG+ IKWNFTKFLID++G VKRYGP P+
Sbjct: 149 IQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
>sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(6P229)
Length = 169
Score = 172 bits (435), Expect = 1e-42
Identities = 83/160 (51%), Positives = 109/160 (68%)
Frame = +1
Query: 70 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 249
SIYDF+ K G ++ LS YKG V++IVNVA + G T NY L E++K D+GL IL
Sbjct: 10 SIYDFTVKDAKGNDVDLSIYKGKVLIIVNVASQCGLTNSNYTDLTEIYKKYKDQGLEILA 69
Query: 250 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDG 429
FPCNQFGGQEP + EI+ V F AE+ +F K++VNGDNA PL+K+LK GF GD
Sbjct: 70 FPCNQFGGQEPGSIEEIQNMVCTRFKAEYPIFDKVDVNGDNAAPLYKFLKSSKGGFFGDS 129
Query: 430 IKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 549
IKWNF+KFL+D+ G V RY PT++P A +E +++K
Sbjct: 130 IKWNFSKFLVDKEGNVVDRYSPTTTP-----ASMEKDIKK 164
>sp|Q4AEH1|GPX4_HYLLA Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 171 bits (434), Expect = 2e-42
Identities = 86/159 (54%), Positives = 113/159 (71%), Gaps = 2/159 (1%)
Frame = +1
Query: 40 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 219
AS D + A S+++FSAK +DG ++L KY+G V ++ NVA + G T NY QL +LH
Sbjct: 30 ASRDDWRCAGSMHEFSAKVLDGHTVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89
Query: 220 LADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLK 399
A+ GLR L FPCNQFG QEP + EIKE+ +N +FD+FSKI VNGD+AHPL+K++K
Sbjct: 90 YAECGLRFLAFPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMK 148
Query: 400 M--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 510
+ K KG LG+ IKWNFTKFL D++G VKRYGP P+
Sbjct: 149 IQPKGKGILGNAIKWNFTKFLFDKNGCVVKRYGPMEEPL 187
>sp|Q91XR9|GPX42_MOUSE Phospholipid hydroperoxide glutathione peroxidase, nuclear (GPX-4)
Length = 253
Score = 171 bits (434), Expect = 2e-42
Identities = 88/162 (54%), Positives = 114/162 (70%), Gaps = 2/162 (1%)
Frame = +1
Query: 28 LSKMASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQE 207
L AS D + A S+++FSAK IDG + L KY+G V ++ NVA + G T NY QL +
Sbjct: 82 LGLCASRDDWRCARSMHEFSAKDIDGHMVCLDKYRGFVCIVTNVASQCGKTDVNYTQLVD 141
Query: 208 LHKLLADKGLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLF 387
LH A+ GLRIL FPCNQFG QEP + EIKE+ +N +FD++SKI VNGD+AHPL+
Sbjct: 142 LHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFA-AGYNVKFDMYSKICVNGDDAHPLW 200
Query: 388 KYLKM--KLKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSP 507
K++K+ K +G LG+ IKWNFTKFLID++G VKRYGP P
Sbjct: 201 KWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCEVKRYGPMEEP 242
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,587,710
Number of Sequences: 369166
Number of extensions: 1804725
Number of successful extensions: 4899
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4592
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4742
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7115329200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)