Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_H10
(681 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX) 125 8e-29
sp|O24296|GPX1_PEA Phospholipid hydroperoxide glutathione p... 99 1e-20
sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione... 96 7e-20
sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione p... 96 7e-20
sp|O02621|GPX1_CAEEL Probable glutathione peroxidase F26E4.12 96 1e-19
sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide gl... 95 1e-19
sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione... 95 1e-19
sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide gl... 95 1e-19
sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione... 95 2e-19
sp|P52032|GPX1_ARATH Phospholipid hydroperoxide glutathione... 94 3e-19
>sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX)
Length = 169
Score = 125 bits (315), Expect = 8e-29
Identities = 58/92 (63%), Positives = 72/92 (78%)
Frame = +2
Query: 404 KNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGL 583
K+ SIY+F+ K I+G ++SL KY+G+V LIVNVACK GAT KNY QLQE+H L KGL
Sbjct: 6 KSWNSIYEFTVKDINGVDVSLEKYRGHVCLIVNVACKCGATDKNYRQLQEMHTRLVGKGL 65
Query: 584 RILGFPCNQFGGQEPWAEPEIKEYVKKNFNAE 679
RIL FPCNQFGGQEPWAE EIK++V + + +
Sbjct: 66 RILAFPCNQFGGQEPWAEAEIKKFVTEKYGVQ 97
>sp|O24296|GPX1_PEA Phospholipid hydroperoxide glutathione peroxidase, chloroplast
precursor (PHGPx)
Length = 236
Score = 99.0 bits (245), Expect = 1e-20
Identities = 46/88 (52%), Positives = 63/88 (71%)
Frame = +2
Query: 416 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 595
+IYDF+ K ID +++SLSK+KG V+LIVNVA + G T+ NY +L L++ +KGL +L
Sbjct: 78 TIYDFTVKDIDKKDVSLSKFKGKVLLIVNVASRCGLTSSNYTELSHLYENFKNKGLEVLA 137
Query: 596 FPCNQFGGQEPWAEPEIKEYVKKNFNAE 679
FPCNQFG QEP + EIK++ F AE
Sbjct: 138 FPCNQFGMQEPGSNEEIKQFACTKFKAE 165
>sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 96.3 bits (238), Expect = 7e-20
Identities = 48/90 (53%), Positives = 63/90 (70%)
Frame = +2
Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
AS D + A S+++FSAK IDG ++L KY+G+V ++ NVA + G T NY QL +LH
Sbjct: 30 ASRDDWRCARSMHEFSAKDIDGRMVNLDKYRGHVCIVTNVASQCGKTDVNYTQLVDLHAR 89
Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEY 655
A+ GLRIL FPCNQFG QEP + EIKE+
Sbjct: 90 YAECGLRILAFPCNQFGRQEPGSNAEIKEF 119
>sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 96.3 bits (238), Expect = 7e-20
Identities = 48/90 (53%), Positives = 63/90 (70%)
Frame = +2
Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
AS D + A S+++FSAK IDG ++L KY+G V ++ NVA + G T NY QL +LH
Sbjct: 30 ASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGYVCIVTNVASQCGKTEVNYTQLVDLHAR 89
Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEY 655
A+ GLRIL FPCNQFG QEP ++ EIKE+
Sbjct: 90 YAECGLRILAFPCNQFGRQEPGSDAEIKEF 119
>sp|O02621|GPX1_CAEEL Probable glutathione peroxidase F26E4.12
Length = 163
Score = 95.5 bits (236), Expect = 1e-19
Identities = 45/89 (50%), Positives = 61/89 (68%)
Frame = +2
Query: 413 TSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRIL 592
+S+YDF+ K +G+++SLS YKG V++IVNVA + G T KNY QL+EL + GL +L
Sbjct: 2 SSVYDFNVKNANGDDVSLSDYKGKVLIIVNVASQCGLTNKNYTQLKELLDVYKKDGLEVL 61
Query: 593 GFPCNQFGGQEPWAEPEIKEYVKKNFNAE 679
FPCNQF GQEP E +I+ +V F E
Sbjct: 62 AFPCNQFAGQEPSCEIDIQAFVADKFKFE 90
>sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(6P229)
Length = 169
Score = 95.1 bits (235), Expect = 1e-19
Identities = 47/88 (53%), Positives = 58/88 (65%)
Frame = +2
Query: 416 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 595
SIYDF+ K G ++ LS YKG V++IVNVA + G T NY L E++K D+GL IL
Sbjct: 10 SIYDFTVKDAKGNDVDLSIYKGKVLIIVNVASQCGLTNSNYTDLTEIYKKYKDQGLEILA 69
Query: 596 FPCNQFGGQEPWAEPEIKEYVKKNFNAE 679
FPCNQFGGQEP + EI+ V F AE
Sbjct: 70 FPCNQFGGQEPGSIEEIQNMVCTRFKAE 97
>sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
sp|P36969|GPX4_HUMAN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 95.1 bits (235), Expect = 1e-19
Identities = 48/90 (53%), Positives = 62/90 (68%)
Frame = +2
Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
AS D + A S+++FSAK IDG ++L KY+G V ++ NVA + G T NY QL +LH
Sbjct: 30 ASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89
Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEY 655
A+ GLRIL FPCNQFG QEP + EIKE+
Sbjct: 90 YAECGLRILAFPCNQFGKQEPGSNEEIKEF 119
>sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Length = 169
Score = 95.1 bits (235), Expect = 1e-19
Identities = 46/91 (50%), Positives = 60/91 (65%)
Frame = +2
Query: 407 NATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLR 586
N S+YDF+ K G+++ LS YKG V++IVNVA + G T NY + EL+K D+GL
Sbjct: 7 NPQSVYDFTVKDAKGKDVDLSIYKGKVLIIVNVASQCGLTNSNYTDMTELYKKYKDQGLE 66
Query: 587 ILGFPCNQFGGQEPWAEPEIKEYVKKNFNAE 679
IL FPCNQFGGQEP +I++ V F AE
Sbjct: 67 ILAFPCNQFGGQEPGNIEDIQQMVCTRFKAE 97
>sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 94.7 bits (234), Expect = 2e-19
Identities = 48/90 (53%), Positives = 62/90 (68%)
Frame = +2
Query: 386 ASSDLVKNATSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKL 565
AS D + A S+++FSAK IDG ++L KY+G V ++ NVA + G T NY QL +LH
Sbjct: 30 ASRDDWRCAGSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHAR 89
Query: 566 LADKGLRILGFPCNQFGGQEPWAEPEIKEY 655
A+ GLRIL FPCNQFG QEP + EIKE+
Sbjct: 90 YAECGLRILAFPCNQFGKQEPGSNEEIKEF 119
>sp|P52032|GPX1_ARATH Phospholipid hydroperoxide glutathione peroxidase, chloroplast
precursor (PHGPx)
Length = 236
Score = 94.4 bits (233), Expect = 3e-19
Identities = 42/88 (47%), Positives = 63/88 (71%)
Frame = +2
Query: 416 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 595
+++DF+ K IDG++++L+K+KG V+LIVNVA + G T+ NY++L L++ +G IL
Sbjct: 78 TVHDFTVKDIDGKDVALNKFKGKVMLIVNVASRCGLTSSNYSELSHLYEKYKTQGFEILA 137
Query: 596 FPCNQFGGQEPWAEPEIKEYVKKNFNAE 679
FPCNQFG QEP + EIK++ F AE
Sbjct: 138 FPCNQFGFQEPGSNSEIKQFACTRFKAE 165
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,111,059
Number of Sequences: 369166
Number of extensions: 1568660
Number of successful extensions: 4174
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3903
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4104
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5782011865
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)