Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_007_P14
(600 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX) 137 2e-32
sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione... 124 2e-28
sp|O23968|GPX4_HELAN Probable phospholipid hydroperoxide gl... 124 2e-28
sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione... 124 2e-28
sp|Q9LEF0|GPX4_MESCR Probable phospholipid hydroperoxide gl... 123 3e-28
sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione... 123 3e-28
sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide gl... 123 4e-28
sp|Q06652|GPX4_CITSI Probable phospholipid hydroperoxide gl... 123 4e-28
sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione p... 123 4e-28
sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide gl... 122 9e-28
>sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX)
Length = 169
Score = 137 bits (345), Expect = 2e-32
Identities = 64/106 (60%), Positives = 81/106 (76%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLK 182
GLRIL FPCNQFGGQEPWAE EIK++V + + +FD+FSKI+VNG +A L+K+LK +
Sbjct: 64 GLRILAFPCNQFGGQEPWAEAEIKKFVTEKYGVQFDMFSKIKVNGSDADDLYKFLKSRQH 123
Query: 183 GFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 320
G L + IKWNF+KFL+DR G PVKRY PT++P D + DI LEK
Sbjct: 124 GTLTNNIKWNFSKFLVDRQGQPVKRYSPTTAPY-DIEGDIMELLEK 168
>sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
sp|P36969|GPX4_HUMAN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 124 bits (311), Expect = 2e-28
Identities = 60/95 (63%), Positives = 74/95 (77%), Gaps = 2/95 (2%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKM--K 176
GLRIL FPCNQFG QEP + EIKE+ +N +FD+FSKI VNGD+AHPL+K++K+ K
Sbjct: 94 GLRILAFPCNQFGKQEPGSNEEIKEFAA-GYNVKFDMFSKICVNGDDAHPLWKWMKIQPK 152
Query: 177 LKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 281
KG LG+ IKWNFTKFLID++G VKRYGP P+
Sbjct: 153 GKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
>sp|O23968|GPX4_HELAN Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(Glutathione peroxidase 2)
Length = 180
Score = 124 bits (310), Expect = 2e-28
Identities = 59/106 (55%), Positives = 73/106 (68%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLK 182
G IL FPCNQFGGQEP + EI+ + F AE+ +FSK+ VNG A PL+K+LK
Sbjct: 75 GFEILAFPCNQFGGQEPGSNEEIQVFACTRFKAEYPVFSKVNVNGKEADPLYKFLKSSKG 134
Query: 183 GFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 320
GFLGD IKWNFTKFL+DR G V RY PT+SP+S IE +++K
Sbjct: 135 GFLGDSIKWNFTKFLVDREGKVVDRYAPTTSPLS-----IEKDIKK 175
>sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 124 bits (310), Expect = 2e-28
Identities = 60/95 (63%), Positives = 74/95 (77%), Gaps = 2/95 (2%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLK 182
GLRIL FPCNQFG QEP + EIKE+ +N +FDLFSKI VNGD+AHPL+K++K++ K
Sbjct: 94 GLRILAFPCNQFGRQEPGSNAEIKEFAA-GYNVKFDLFSKICVNGDDAHPLWKWMKVQPK 152
Query: 183 G--FLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPI 281
G LG+ IKWNFTKFLID++G VKRYGP P+
Sbjct: 153 GRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
>sp|Q9LEF0|GPX4_MESCR Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Length = 170
Score = 123 bits (309), Expect = 3e-28
Identities = 59/106 (55%), Positives = 71/106 (66%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLK 182
GL IL FPCNQFG QEP +I E+ F AEF +F K++VNG NA P++KYLK
Sbjct: 65 GLEILAFPCNQFGNQEPGDNEQIMEFACTRFKAEFPIFDKVDVNGSNAAPVYKYLKSSKG 124
Query: 183 GFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 320
G GDGIKWNFTKFL+DR G V RY PT+SP A IE +++K
Sbjct: 125 GLFGDGIKWNFTKFLVDRDGKVVDRYAPTTSP-----ASIEKDIKK 165
>sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 123 bits (309), Expect = 3e-28
Identities = 60/94 (63%), Positives = 73/94 (77%), Gaps = 2/94 (2%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKM--K 176
GLRIL FPCNQFG QEP + EIKE+ +N +FD+FSKI VNGD+AHPL+K++K+ K
Sbjct: 94 GLRILAFPCNQFGKQEPGSNEEIKEFAA-GYNVKFDMFSKICVNGDDAHPLWKWMKIQPK 152
Query: 177 LKGFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSP 278
KG LG+ IKWNFTKFLID++G VKRYGP P
Sbjct: 153 GKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEP 186
>sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Length = 169
Score = 123 bits (308), Expect = 4e-28
Identities = 58/106 (54%), Positives = 75/106 (70%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLK 182
GL IL FPCNQFGGQEP +I++ V F AE+ +F K++VNGDNA PL+++LK
Sbjct: 64 GLEILAFPCNQFGGQEPGNIEDIQQMVCTRFKAEYPIFDKVDVNGDNAAPLYRFLKSSKG 123
Query: 183 GFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 320
GF GDGIKWNF+KFLID+ G V RY PT+SP A +E +++K
Sbjct: 124 GFFGDGIKWNFSKFLIDKEGHVVDRYSPTTSP-----ASMEKDIKK 164
>sp|Q06652|GPX4_CITSI Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(Salt-associated protein)
Length = 167
Score = 123 bits (308), Expect = 4e-28
Identities = 58/105 (55%), Positives = 74/105 (70%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLK 182
GL IL FPCNQFG QEP +I+E+ F AEF +F K++VNGDNA PL+K+LK
Sbjct: 62 GLEILAFPCNQFGAQEPGDNEQIQEFACTRFKAEFPIFDKVDVNGDNAAPLYKHLKSSKG 121
Query: 183 GFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELE 317
G GD IKWNF+KFL+D+ G V+RY PT+SP+S + DI+ LE
Sbjct: 122 GLFGDSIKWNFSKFLVDKEGNVVERYAPTTSPLS-IEKDIKKLLE 165
>sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 123 bits (308), Expect = 4e-28
Identities = 59/94 (62%), Positives = 74/94 (78%), Gaps = 2/94 (2%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLK 182
GLRIL FPCNQFG QEP ++ EIKE+ +N +FD+FSKI VNGD+AHPL+K++K++ K
Sbjct: 94 GLRILAFPCNQFGRQEPGSDAEIKEFAA-GYNVKFDMFSKICVNGDDAHPLWKWMKVQPK 152
Query: 183 G--FLGDGIKWNFTKFLIDRHGIPVKRYGPTSSP 278
G LG+ IKWNFTKFLID++G VKRYGP P
Sbjct: 153 GRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEP 186
>sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(6P229)
Length = 169
Score = 122 bits (305), Expect = 9e-28
Identities = 57/106 (53%), Positives = 74/106 (69%)
Frame = +3
Query: 3 GLRILGFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLK 182
GL IL FPCNQFGGQEP + EI+ V F AE+ +F K++VNGDNA PL+K+LK
Sbjct: 64 GLEILAFPCNQFGGQEPGSIEEIQNMVCTRFKAEYPIFDKVDVNGDNAAPLYKFLKSSKG 123
Query: 183 GFLGDGIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 320
GF GD IKWNF+KFL+D+ G V RY PT++P A +E +++K
Sbjct: 124 GFFGDSIKWNFSKFLVDKEGNVVDRYSPTTTP-----ASMEKDIKK 164
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,813,272
Number of Sequences: 369166
Number of extensions: 1265274
Number of successful extensions: 3647
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3460
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3538
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4602033670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)