Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_M22
(731 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX) 186 7e-47
sp|Q9LEF0|GPX4_MESCR Probable phospholipid hydroperoxide gl... 155 8e-38
sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide gl... 155 1e-37
sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide gl... 154 2e-37
sp|O23968|GPX4_HELAN Probable phospholipid hydroperoxide gl... 154 3e-37
sp|Q9FXS3|GPX4_TOBAC Probable phospholipid hydroperoxide gl... 153 4e-37
sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione... 153 5e-37
sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione... 152 9e-37
sp|Q06652|GPX4_CITSI Probable phospholipid hydroperoxide gl... 151 2e-36
sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione... 151 2e-36
>sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX)
Length = 169
Score = 186 bits (471), Expect = 7e-47
Identities = 89/143 (62%), Positives = 110/143 (76%)
Frame = +3
Query: 6 EQYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEI 185
E+Y+G+V LIVNVACK GAT KNY QLQE+H L KGLRIL FPCNQFGGQEPWAE EI
Sbjct: 27 EKYRGHVCLIVNVACKCGATDKNYRQLQEMHTRLVGKGLRILAFPCNQFGGQEPWAEAEI 86
Query: 186 KEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDGIKWNFTKFLIDRHGIPV 365
K++V + + +FD+FSKI+VNG +A L+K+LK + G L + IKWNF+KFL+DR G PV
Sbjct: 87 KKFVTEKYGVQFDMFSKIKVNGSDADDLYKFLKSRQHGTLTNNIKWNFSKFLVDRQGQPV 146
Query: 366 KRYGPTSSPISDCKADIESELEK 434
KRY PT++P D + DI LEK
Sbjct: 147 KRYSPTTAPY-DIEGDIMELLEK 168
>sp|Q9LEF0|GPX4_MESCR Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Length = 170
Score = 155 bits (393), Expect = 8e-38
Identities = 77/141 (54%), Positives = 95/141 (67%)
Frame = +3
Query: 12 YKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEIKE 191
YKG V+LIVNVA + G T NY +L +L++ DKGL IL FPCNQFG QEP +I E
Sbjct: 30 YKGKVLLIVNVASQCGLTNSNYPELTKLYEQYKDKGLEILAFPCNQFGNQEPGDNEQIME 89
Query: 192 YVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDGIKWNFTKFLIDRHGIPVKR 371
+ F AEF +F K++VNG NA P++KYLK G GDGIKWNFTKFL+DR G V R
Sbjct: 90 FACTRFKAEFPIFDKVDVNGSNAAPVYKYLKSSKGGLFGDGIKWNFTKFLVDRDGKVVDR 149
Query: 372 YGPTSSPISDCKADIESELEK 434
Y PT+SP A IE +++K
Sbjct: 150 YAPTTSP-----ASIEKDIKK 165
>sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Length = 169
Score = 155 bits (392), Expect = 1e-37
Identities = 75/141 (53%), Positives = 98/141 (69%)
Frame = +3
Query: 12 YKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEIKE 191
YKG V++IVNVA + G T NY + EL+K D+GL IL FPCNQFGGQEP +I++
Sbjct: 29 YKGKVLIIVNVASQCGLTNSNYTDMTELYKKYKDQGLEILAFPCNQFGGQEPGNIEDIQQ 88
Query: 192 YVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDGIKWNFTKFLIDRHGIPVKR 371
V F AE+ +F K++VNGDNA PL+++LK GF GDGIKWNF+KFLID+ G V R
Sbjct: 89 MVCTRFKAEYPIFDKVDVNGDNAAPLYRFLKSSKGGFFGDGIKWNFSKFLIDKEGHVVDR 148
Query: 372 YGPTSSPISDCKADIESELEK 434
Y PT+SP A +E +++K
Sbjct: 149 YSPTTSP-----ASMEKDIKK 164
>sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(6P229)
Length = 169
Score = 154 bits (389), Expect = 2e-37
Identities = 74/141 (52%), Positives = 97/141 (68%)
Frame = +3
Query: 12 YKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEIKE 191
YKG V++IVNVA + G T NY L E++K D+GL IL FPCNQFGGQEP + EI+
Sbjct: 29 YKGKVLIIVNVASQCGLTNSNYTDLTEIYKKYKDQGLEILAFPCNQFGGQEPGSIEEIQN 88
Query: 192 YVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDGIKWNFTKFLIDRHGIPVKR 371
V F AE+ +F K++VNGDNA PL+K+LK GF GD IKWNF+KFL+D+ G V R
Sbjct: 89 MVCTRFKAEYPIFDKVDVNGDNAAPLYKFLKSSKGGFFGDSIKWNFSKFLVDKEGNVVDR 148
Query: 372 YGPTSSPISDCKADIESELEK 434
Y PT++P A +E +++K
Sbjct: 149 YSPTTTP-----ASMEKDIKK 164
>sp|O23968|GPX4_HELAN Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(Glutathione peroxidase 2)
Length = 180
Score = 154 bits (388), Expect = 3e-37
Identities = 76/142 (53%), Positives = 97/142 (68%)
Frame = +3
Query: 9 QYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEIK 188
+YKG V+LIVNVA + G T NY +L L++ D+G IL FPCNQFGGQEP + EI+
Sbjct: 39 KYKGKVLLIVNVASQCGFTNSNYPELTTLYQKYKDQGFEILAFPCNQFGGQEPGSNEEIQ 98
Query: 189 EYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDGIKWNFTKFLIDRHGIPVK 368
+ F AE+ +FSK+ VNG A PL+K+LK GFLGD IKWNFTKFL+DR G V
Sbjct: 99 VFACTRFKAEYPVFSKVNVNGKEADPLYKFLKSSKGGFLGDSIKWNFTKFLVDREGKVVD 158
Query: 369 RYGPTSSPISDCKADIESELEK 434
RY PT+SP+S IE +++K
Sbjct: 159 RYAPTTSPLS-----IEKDIKK 175
>sp|Q9FXS3|GPX4_TOBAC Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(Nt-SubC08)
Length = 169
Score = 153 bits (387), Expect = 4e-37
Identities = 73/141 (51%), Positives = 97/141 (68%)
Frame = +3
Query: 12 YKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEIKE 191
YKG V++IVNVA + G T NY + E++K D+GL IL FPCNQFGGQEP + EI+
Sbjct: 29 YKGKVLIIVNVASQCGLTNSNYTDMTEIYKKYKDQGLEILAFPCNQFGGQEPGSIEEIQN 88
Query: 192 YVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDGIKWNFTKFLIDRHGIPVKR 371
V F AE+ +F K++VNGDNA PL+K+LK GF GD IKWNF+KFL+D+ G V R
Sbjct: 89 MVCTRFKAEYPIFDKVDVNGDNAAPLYKFLKSSKGGFFGDSIKWNFSKFLVDKEGNVVDR 148
Query: 372 YGPTSSPISDCKADIESELEK 434
Y PT++P A +E +++K
Sbjct: 149 YSPTTTP-----ASMEKDIKK 164
>sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 153 bits (386), Expect = 5e-37
Identities = 75/132 (56%), Positives = 98/132 (74%), Gaps = 2/132 (1%)
Frame = +3
Query: 6 EQYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEI 185
++Y+G+V ++ NVA + G T NY QL +LH A+ GLRIL FPCNQFG QEP + EI
Sbjct: 57 DKYRGHVCIVTNVASQCGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNAEI 116
Query: 186 KEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKM--KLKGFLGDGIKWNFTKFLIDRHGI 359
KE+ +N +FDLFSKI VNGD+AHPL+K++K+ K +G LG+ IKWNFTKFLID++G
Sbjct: 117 KEFA-AGYNVKFDLFSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGC 175
Query: 360 PVKRYGPTSSPI 395
VKRYGP P+
Sbjct: 176 VVKRYGPMEEPL 187
>sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
sp|P36969|GPX4_HUMAN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 152 bits (384), Expect = 9e-37
Identities = 75/132 (56%), Positives = 97/132 (73%), Gaps = 2/132 (1%)
Frame = +3
Query: 6 EQYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEI 185
++Y+G V ++ NVA + G T NY QL +LH A+ GLRIL FPCNQFG QEP + EI
Sbjct: 57 DKYRGFVCIVTNVASQCGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEI 116
Query: 186 KEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKM--KLKGFLGDGIKWNFTKFLIDRHGI 359
KE+ +N +FD+FSKI VNGD+AHPL+K++K+ K KG LG+ IKWNFTKFLID++G
Sbjct: 117 KEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGC 175
Query: 360 PVKRYGPTSSPI 395
VKRYGP P+
Sbjct: 176 VVKRYGPMEEPL 187
>sp|Q06652|GPX4_CITSI Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(Salt-associated protein)
Length = 167
Score = 151 bits (382), Expect = 2e-36
Identities = 73/140 (52%), Positives = 97/140 (69%)
Frame = +3
Query: 12 YKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEIKE 191
YKG ++LIVNVA + G T NY +L +L+ ++GL IL FPCNQFG QEP +I+E
Sbjct: 27 YKGKLLLIVNVASQCGLTNSNYTELSQLYDKYKNQGLEILAFPCNQFGAQEPGDNEQIQE 86
Query: 192 YVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDGIKWNFTKFLIDRHGIPVKR 371
+ F AEF +F K++VNGDNA PL+K+LK G GD IKWNF+KFL+D+ G V+R
Sbjct: 87 FACTRFKAEFPIFDKVDVNGDNAAPLYKHLKSSKGGLFGDSIKWNFSKFLVDKEGNVVER 146
Query: 372 YGPTSSPISDCKADIESELE 431
Y PT+SP+S + DI+ LE
Sbjct: 147 YAPTTSPLS-IEKDIKKLLE 165
>sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 151 bits (382), Expect = 2e-36
Identities = 75/131 (57%), Positives = 96/131 (73%), Gaps = 2/131 (1%)
Frame = +3
Query: 6 EQYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILGFPCNQFGGQEPWAEPEI 185
++Y+G V ++ NVA + G T NY QL +LH A+ GLRIL FPCNQFG QEP + EI
Sbjct: 57 DKYRGFVCIVTNVASQCGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEI 116
Query: 186 KEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKM--KLKGFLGDGIKWNFTKFLIDRHGI 359
KE+ +N +FD+FSKI VNGD+AHPL+K++K+ K KG LG+ IKWNFTKFLID++G
Sbjct: 117 KEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGC 175
Query: 360 PVKRYGPTSSP 392
VKRYGP P
Sbjct: 176 VVKRYGPMEEP 186
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,160,693
Number of Sequences: 369166
Number of extensions: 1631819
Number of successful extensions: 4453
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4306
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6534486000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)