Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_P22
(628 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX) 204 1e-52
sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide gl... 173 4e-43
sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione... 172 5e-43
sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide gl... 172 8e-43
sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione... 172 8e-43
sp|Q9FXS3|GPX4_TOBAC Probable phospholipid hydroperoxide gl... 171 1e-42
sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione... 171 1e-42
sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione p... 171 1e-42
sp|Q9LEF0|GPX4_MESCR Probable phospholipid hydroperoxide gl... 170 3e-42
sp|Q06652|GPX4_CITSI Probable phospholipid hydroperoxide gl... 169 4e-42
>sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX)
Length = 169
Score = 204 bits (520), Expect = 1e-52
Identities = 98/160 (61%), Positives = 123/160 (76%)
Frame = +2
Query: 5 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 184
SIY+F+ K I+G ++SL KY+G+V LIVNVACK GAT KNY QLQE+H L KGLRIL
Sbjct: 10 SIYEFTVKDINGVDVSLEKYRGHVCLIVNVACKCGATDKNYRQLQEMHTRLVGKGLRILA 69
Query: 185 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDG 364
FPCNQFGGQEPWAE EIK++V + + +FD+FSKI+VNG +A L+K+LK + G L +
Sbjct: 70 FPCNQFGGQEPWAEAEIKKFVTEKYGVQFDMFSKIKVNGSDADDLYKFLKSRQHGTLTNN 129
Query: 365 IKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 484
IKWNF+KFL+DR G PVKRY PT++P D + DI LEK
Sbjct: 130 IKWNFSKFLVDRQGQPVKRYSPTTAPY-DIEGDIMELLEK 168
>sp|O24031|GPX4_LYCES Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Length = 169
Score = 173 bits (438), Expect = 4e-43
Identities = 83/160 (51%), Positives = 111/160 (69%)
Frame = +2
Query: 5 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 184
S+YDF+ K G+++ LS YKG V++IVNVA + G T NY + EL+K D+GL IL
Sbjct: 10 SVYDFTVKDAKGKDVDLSIYKGKVLIIVNVASQCGLTNSNYTDMTELYKKYKDQGLEILA 69
Query: 185 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDG 364
FPCNQFGGQEP +I++ V F AE+ +F K++VNGDNA PL+++LK GF GDG
Sbjct: 70 FPCNQFGGQEPGNIEDIQQMVCTRFKAEYPIFDKVDVNGDNAAPLYRFLKSSKGGFFGDG 129
Query: 365 IKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 484
IKWNF+KFLID+ G V RY PT+SP A +E +++K
Sbjct: 130 IKWNFSKFLIDKEGHVVDRYSPTTSP-----ASMEKDIKK 164
>sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 172 bits (437), Expect = 5e-43
Identities = 85/149 (57%), Positives = 111/149 (74%), Gaps = 2/149 (1%)
Frame = +2
Query: 5 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 184
S+++FSAK IDG ++L KY+G+V ++ NVA + G T NY QL +LH A+ GLRIL
Sbjct: 40 SMHEFSAKDIDGRMVNLDKYRGHVCIVTNVASQCGKTDVNYTQLVDLHARYAECGLRILA 99
Query: 185 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKM--KLKGFLG 358
FPCNQFG QEP + EIKE+ +N +FDLFSKI VNGD+AHPL+K++K+ K +G LG
Sbjct: 100 FPCNQFGRQEPGSNAEIKEFA-AGYNVKFDLFSKICVNGDDAHPLWKWMKVQPKGRGMLG 158
Query: 359 DGIKWNFTKFLIDRHGIPVKRYGPTSSPI 445
+ IKWNFTKFLID++G VKRYGP P+
Sbjct: 159 NAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
>sp|P30708|GPX4_NICSY Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(6P229)
Length = 169
Score = 172 bits (435), Expect = 8e-43
Identities = 83/160 (51%), Positives = 109/160 (68%)
Frame = +2
Query: 5 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 184
SIYDF+ K G ++ LS YKG V++IVNVA + G T NY L E++K D+GL IL
Sbjct: 10 SIYDFTVKDAKGNDVDLSIYKGKVLIIVNVASQCGLTNSNYTDLTEIYKKYKDQGLEILA 69
Query: 185 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDG 364
FPCNQFGGQEP + EI+ V F AE+ +F K++VNGDNA PL+K+LK GF GD
Sbjct: 70 FPCNQFGGQEPGSIEEIQNMVCTRFKAEYPIFDKVDVNGDNAAPLYKFLKSSKGGFFGDS 129
Query: 365 IKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 484
IKWNF+KFL+D+ G V RY PT++P A +E +++K
Sbjct: 130 IKWNFSKFLVDKEGNVVDRYSPTTTP-----ASMEKDIKK 164
>sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
sp|P36969|GPX4_HUMAN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 172 bits (435), Expect = 8e-43
Identities = 85/149 (57%), Positives = 110/149 (73%), Gaps = 2/149 (1%)
Frame = +2
Query: 5 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 184
S+++FSAK IDG ++L KY+G V ++ NVA + G T NY QL +LH A+ GLRIL
Sbjct: 40 SMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHARYAECGLRILA 99
Query: 185 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKM--KLKGFLG 358
FPCNQFG QEP + EIKE+ +N +FD+FSKI VNGD+AHPL+K++K+ K KG LG
Sbjct: 100 FPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILG 158
Query: 359 DGIKWNFTKFLIDRHGIPVKRYGPTSSPI 445
+ IKWNFTKFLID++G VKRYGP P+
Sbjct: 159 NAIKWNFTKFLIDKNGCVVKRYGPMEEPL 187
>sp|Q9FXS3|GPX4_TOBAC Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(Nt-SubC08)
Length = 169
Score = 171 bits (433), Expect = 1e-42
Identities = 82/160 (51%), Positives = 109/160 (68%)
Frame = +2
Query: 5 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 184
SIYDF+ K G ++ LS YKG V++IVNVA + G T NY + E++K D+GL IL
Sbjct: 10 SIYDFTVKDAKGNDVDLSIYKGKVLIIVNVASQCGLTNSNYTDMTEIYKKYKDQGLEILA 69
Query: 185 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDG 364
FPCNQFGGQEP + EI+ V F AE+ +F K++VNGDNA PL+K+LK GF GD
Sbjct: 70 FPCNQFGGQEPGSIEEIQNMVCTRFKAEYPIFDKVDVNGDNAAPLYKFLKSSKGGFFGDS 129
Query: 365 IKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 484
IKWNF+KFL+D+ G V RY PT++P A +E +++K
Sbjct: 130 IKWNFSKFLVDKEGNVVDRYSPTTTP-----ASMEKDIKK 164
>sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 171 bits (433), Expect = 1e-42
Identities = 85/148 (57%), Positives = 109/148 (73%), Gaps = 2/148 (1%)
Frame = +2
Query: 5 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 184
S+++FSAK IDG ++L KY+G V ++ NVA + G T NY QL +LH A+ GLRIL
Sbjct: 40 SMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHARYAECGLRILA 99
Query: 185 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKM--KLKGFLG 358
FPCNQFG QEP + EIKE+ +N +FD+FSKI VNGD+AHPL+K++K+ K KG LG
Sbjct: 100 FPCNQFGKQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILG 158
Query: 359 DGIKWNFTKFLIDRHGIPVKRYGPTSSP 442
+ IKWNFTKFLID++G VKRYGP P
Sbjct: 159 NAIKWNFTKFLIDKNGCVVKRYGPMEEP 186
>sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 171 bits (433), Expect = 1e-42
Identities = 84/148 (56%), Positives = 110/148 (74%), Gaps = 2/148 (1%)
Frame = +2
Query: 5 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 184
S+++FSAK IDG ++L KY+G V ++ NVA + G T NY QL +LH A+ GLRIL
Sbjct: 40 SMHEFSAKDIDGHMVNLDKYRGYVCIVTNVASQCGKTEVNYTQLVDLHARYAECGLRILA 99
Query: 185 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKM--KLKGFLG 358
FPCNQFG QEP ++ EIKE+ +N +FD+FSKI VNGD+AHPL+K++K+ K +G LG
Sbjct: 100 FPCNQFGRQEPGSDAEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKVQPKGRGMLG 158
Query: 359 DGIKWNFTKFLIDRHGIPVKRYGPTSSP 442
+ IKWNFTKFLID++G VKRYGP P
Sbjct: 159 NAIKWNFTKFLIDKNGCVVKRYGPMEEP 186
>sp|Q9LEF0|GPX4_MESCR Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
Length = 170
Score = 170 bits (430), Expect = 3e-42
Identities = 85/160 (53%), Positives = 106/160 (66%)
Frame = +2
Query: 5 SIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRILG 184
SI+DF K G ++ LS YKG V+LIVNVA + G T NY +L +L++ DKGL IL
Sbjct: 11 SIHDFIVKDARGNDVDLSIYKGKVLLIVNVASQCGLTNSNYPELTKLYEQYKDKGLEILA 70
Query: 185 FPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGDG 364
FPCNQFG QEP +I E+ F AEF +F K++VNG NA P++KYLK G GDG
Sbjct: 71 FPCNQFGNQEPGDNEQIMEFACTRFKAEFPIFDKVDVNGSNAAPVYKYLKSSKGGLFGDG 130
Query: 365 IKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELEK 484
IKWNFTKFL+DR G V RY PT+SP A IE +++K
Sbjct: 131 IKWNFTKFLVDRDGKVVDRYAPTTSP-----ASIEKDIKK 165
>sp|Q06652|GPX4_CITSI Probable phospholipid hydroperoxide glutathione peroxidase (PHGPx)
(Salt-associated protein)
Length = 167
Score = 169 bits (429), Expect = 4e-42
Identities = 81/160 (50%), Positives = 111/160 (69%)
Frame = +2
Query: 2 TSIYDFSAKTIDGEEISLSKYKGNVVLIVNVACK*GATAKNYNQLQELHKLLADKGLRIL 181
TS++DF+ K G+++ LS YKG ++LIVNVA + G T NY +L +L+ ++GL IL
Sbjct: 7 TSVHDFTVKDAKGQDVDLSIYKGKLLLIVNVASQCGLTNSNYTELSQLYDKYKNQGLEIL 66
Query: 182 GFPCNQFGGQEPWAEPEIKEYVKKNFNAEFDLFSKIEVNGDNAHPLFKYLKMKLKGFLGD 361
FPCNQFG QEP +I+E+ F AEF +F K++VNGDNA PL+K+LK G GD
Sbjct: 67 AFPCNQFGAQEPGDNEQIQEFACTRFKAEFPIFDKVDVNGDNAAPLYKHLKSSKGGLFGD 126
Query: 362 GIKWNFTKFLIDRHGIPVKRYGPTSSPISDCKADIESELE 481
IKWNF+KFL+D+ G V+RY PT+SP+S + DI+ LE
Sbjct: 127 SIKWNFSKFLVDKEGNVVERYAPTTSPLS-IEKDIKKLLE 165
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,853,762
Number of Sequences: 369166
Number of extensions: 1529433
Number of successful extensions: 4375
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4220
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4974853140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)