Planaria EST Database


DrC_00620

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00620
         (1285 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9VCI3|LSD1_DROME  Lipid storage droplets surface binding...    55   4e-07
sp|Q9VXY7|LSD2_DROME  Lipid storage droplets surface binding...    49   3e-05
sp|P43883|ADFP_MOUSE  Adipophilin (Adipose differentiation-r...    47   1e-04
sp|Q9TUM6|ADFP_BOVIN  Adipophilin (Adipose differentiation-r...    44   0.001
sp|Q9N4M4|ANC1_CAEEL  Nuclear anchorage protein 1 (Anchorage...    37   0.095
sp|Q6FS63|MAD1_CANGA  Spindle assembly checkpoint component ...    35   0.36 
sp|O67358|TIG_AQUAE  Trigger factor (TF)                           35   0.47 
sp|P39723|YAE7_YEAST  Hypothetical 72.1 kDa protein in ACS1-...    34   0.80 
sp|Q93Y94|RPO1_NICSY  DNA-directed RNA polymerase 1, mitocho...    34   1.0  
sp|Q27713|DRTS_PLABA  Bifunctional dihydrofolate reductase-t...    34   1.0  
>sp|Q9VCI3|LSD1_DROME Lipid storage droplets surface binding protein 1
          Length = 431

 Score = 55.1 bits (131), Expect = 4e-07
 Identities = 64/269 (23%), Positives = 109/269 (40%), Gaps = 35/269 (13%)
 Frame = +3

Query: 33  ASSHSDNHFKSLDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTA 212
           A+S S  H +++DR+   P+V  S++ V + Y  +K++N +       AE T+ A  +T 
Sbjct: 4   ATSGSGLHLEAIDRIGSIPLVESSVKRVETIYDKVKNNNRLFSWYFETAEATISAAYETI 63

Query: 213 EPIAVR----QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLM 380
           +P AV+     +  +DN +   LD  E++ P++              Y ++ E     L+
Sbjct: 64  QP-AVKLFEPSIQRLDNVMCKSLDILEQRIPLV-------YLPPEMMYWNTKEYMSDHLV 115

Query: 381 WPVTKTLDTV---------SKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNYVLDKTD 533
            PV K  D+V         S  + +A            K V    YL  + T+   D+TD
Sbjct: 116 RPVLKRADSVKQIGNAVLESPLTTYAAERIDGAFTVGDKFV--DKYLVPIQTDQ--DQTD 171

Query: 534 ------ELLDKYLP----------------DNNQCNEEPYSNADIVTKVKRLSGKTQQRL 647
                  L D + P                D+N+   +       +   +R S K ++RL
Sbjct: 172 VKKLVSALNDNFSPIRQQLNRMFGHKSPQEDDNEAVPDERGAIKAIHHGQRFSRKLKRRL 231

Query: 648 YKRASEQINALLKASLEKLHQIHVLIEEI 734
            +R   +  AL K S E +H +    E I
Sbjct: 232 TQRTIAEARALKKQSKEAIHVLFYAAELI 260
>sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding protein 2 (Lsd2)
          Length = 352

 Score = 48.9 bits (115), Expect = 3e-05
 Identities = 59/263 (22%), Positives = 95/263 (36%), Gaps = 44/263 (16%)
 Frame = +3

Query: 54  HFKSLDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAV-- 227
           H +SL+R+   P+VN + +     Y  +K  N + +  LT AE  +     TA P     
Sbjct: 36  HLESLERIIKLPVVNAAWDKSQDVYGKVKGKNRVFEWALTAAEDCVTRAVTTAAPFVTKL 95

Query: 228 -RQLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWPVTKTLD 404
            R + ++D  ++  +D+ E K PII +              D V+   + ++        
Sbjct: 96  DRPIAYVDQTLVKGIDKLEVKAPIIKDTPQEIYNQAKSKVIDVVQPHLERVVKFKAAGQQ 155

Query: 405 TVSKTSDFAXXXXXXXXXXXMKIVLPATY----LTNVSTNYVLDKTDELLDKYLP----- 557
             +   D A              VL   Y    +  V T   L   + LL+ Y P     
Sbjct: 156 KAASLKDLAWQKANE--------VLATQYGSLAVNGVDTTTAL--AERLLEYYFPKCESD 205

Query: 558 ---DNNQ-------------------CNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQI 671
              DN+                     +E+P  +   V  V RLS K  +R+Y+  S QI
Sbjct: 206 VEEDNDDKQNAVVQNGKSSENDMPVPASEDPVLHT--VQTVGRLSNKISRRVYRNVSRQI 263

Query: 672 NALLKASLE----------KLHQ 710
             + K ++           KLHQ
Sbjct: 264 KQVQKGNINDYLSSLIAALKLHQ 286
>sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-related protein) (ADRP)
          Length = 425

 Score = 47.0 bits (110), Expect = 1e-04
 Identities = 52/254 (20%), Positives = 94/254 (37%), Gaps = 23/254 (9%)
 Frame = +3

Query: 72  RVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAV-------R 230
           RV + P+V+ + + V S Y   K     L +    AEK +K V   A   A+        
Sbjct: 15  RVANLPLVSSTYDLVSSAYVSTKDQYPYLRSVCEMAEKGVKTVTSAAMTSALPIIQKLEP 74

Query: 231 QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWPVTKTLDTV 410
           Q+   +      LDR E++ PI+ +               + +    T+         TV
Sbjct: 75  QIAVANTYACKGLDRMEERLPILNQPTSEIVASARGAVTGAKDVVTTTMAGAKDSVASTV 134

Query: 411 SKTSDFAXXXXXXXXXXXMKI-------VLPATYLTNVSTNYVLDKTDELLDKYLP---D 560
           S   D               +       VL      N   +  + K++ L+D+Y P   +
Sbjct: 135 SGVVDKTKGAVTGSVERTKSVVNGSINTVLGMVQFMNSGVDNAITKSEMLVDQYFPLTQE 194

Query: 561 NNQCNEEPYSNADIVTK------VKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVL 722
             +   +     D+V K      ++ LS K   R Y +A  ++    + S E + Q+H  
Sbjct: 195 ELEMEAKKVEGFDMVQKPSNYERLESLSTKLCSRAYHQALSRVKEAKQKSQETISQLHST 254

Query: 723 IEEINKRREDLHNS 764
           +  I   R+++H++
Sbjct: 255 VHLIEFARKNMHSA 268
>sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-related protein) (ADRP)
          Length = 450

 Score = 43.9 bits (102), Expect = 0.001
 Identities = 57/270 (21%), Positives = 106/270 (39%), Gaps = 25/270 (9%)
 Frame = +3

Query: 30  VASSHSDNHFKSLDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDT 209
           +AS   +     + RV + P+V+ + + V S Y   K     L +    AEK +K +   
Sbjct: 1   MASVAVEPQLSVVTRVANLPLVSSTYDLVSSAYISRKDQYPYLKSLCEMAEKGMKTITSV 60

Query: 210 AEPIAVR-------QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXY---HDSVE 359
           A   A+        Q+   +      LDR E+K PI+ +                 D+V 
Sbjct: 61  AVTSALPIIQKLEPQIAVANTYACKGLDRIEEKLPILNQPTNQVVANAKGAMTGAKDAVT 120

Query: 360 ST----KKTLMWPVTKTLDTVSKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNY--VL 521
           +T    K ++   +T  +D        +           +  VL +  +  +S+     L
Sbjct: 121 TTVTGAKDSVASTITGVVDRTKGAVTGSVEKTKSVVSGSINTVLRSRVMQLMSSGVENAL 180

Query: 522 DKTDELLDKYLP---DNNQCNEEPYSNADIVTK------VKRLSGKTQQRLYKRASEQIN 674
            K++ L+D+YLP   D  +   +     D+V K      +  LS K + R Y++A  ++ 
Sbjct: 181 TKSELLVDQYLPLTKDELEKEAKKVEGFDMVQKPSYYVRLGSLSTKLRSRAYQQALCRVE 240

Query: 675 ALLKASLEKLHQIHVLIEEINKRREDLHNS 764
              +   E + Q+H         R+++HN+
Sbjct: 241 EAKRKGQETISQLHSAFNLSELARKNVHNA 270
>sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog)
          Length = 8545

 Score = 37.4 bits (85), Expect = 0.095
 Identities = 25/99 (25%), Positives = 51/99 (51%), Gaps = 1/99 (1%)
 Frame = +3

Query: 639  QRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPF 818
            +R Y+   +++NA + A +E L  + +L  E+++ +ED++N      S  +D + +    
Sbjct: 8289 ERQYEDTMDKLNAEITAEVELLRTLDILSNELSQCKEDINNP-----SVDVDELSRATML 8343

Query: 819  N-LINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNNA 932
            N  I H +     +  S+  +  V SSTS+ L+ ++  A
Sbjct: 8344 NDAIAHLENQKVVVARSEKDRKFVESSTSIDLDQLLAEA 8382
>sp|Q6FS63|MAD1_CANGA Spindle assembly checkpoint component MAD1 (Mitotic arrest deficient
            protein 1)
          Length = 657

 Score = 35.4 bits (80), Expect = 0.36
 Identities = 44/202 (21%), Positives = 78/202 (38%)
 Frame = +3

Query: 504  STNYVLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALL 683
            STN    + D LLD Y  +      E     D + +  R  G   ++   R  + IN   
Sbjct: 382  STNRNDAEYDSLLDNYKQETENLTNELKRLNDSLIEQNRNQGTEMKKRKLRNGDHININY 441

Query: 684  KASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALRY 863
               L +L Q ++ +E+ N++       V ++    LD ++K  P N+    Q  S  L+ 
Sbjct: 442  SQRLNELQQNNLSLEKDNEKL----RLVVEKLEGKLDDLRKTKPKNIRILQQRDSPLLK- 496

Query: 864  SKSLKNVVSSSTSVTLEYMVNNALNGLDIVNDNLFKLQNFLQQARPESVTGELLIHTSTA 1043
                           L+++       L   N NL  +   + + R ES     L   ++ 
Sbjct: 497  ---------------LQFVRRKENELLREENQNLLTMIENITENRNESFDKIPLTSYNSL 541

Query: 1044 VTEIRIALDSFIYHMKRYSRKK 1109
              +  I++D+     K++SR K
Sbjct: 542  KYQYEISMDTNKKQEKKFSRLK 563
>sp|O67358|TIG_AQUAE Trigger factor (TF)
          Length = 478

 Score = 35.0 bits (79), Expect = 0.47
 Identities = 33/130 (25%), Positives = 61/130 (46%), Gaps = 5/130 (3%)
 Frame = +3

Query: 516 VLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASL 695
           V DK  E+ D  +P      E  +     + +++ L   T+    K+  E++  + +A++
Sbjct: 321 VADKLVEIHDIPVPQTLLRRELSFLVDRRLRELQALGIDTRYVDIKKIVEEVQPIAEANI 380

Query: 696 EKLHQIHVLIEE--INKRREDL---HNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALR 860
           +    +    +E  I    ED+   +  +A++Y  T+D IKK   F   N  Q+V E  R
Sbjct: 381 KLRFILDKYAQEKGIEPTGEDIEAQYKELAEQYGTTVDEIKKY--FKENNLEQVVYEDAR 438

Query: 861 YSKSLKNVVS 890
             K+LK ++S
Sbjct: 439 RKKALKEIIS 448
>sp|P39723|YAE7_YEAST Hypothetical 72.1 kDa protein in ACS1-GCV3 intergenic region
          Length = 622

 Score = 34.3 bits (77), Expect = 0.80
 Identities = 34/142 (23%), Positives = 56/142 (39%), Gaps = 12/142 (8%)
 Frame = +3

Query: 606 TKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSN 785
           TK+ +L             +QIN L    ++K  + HV+  +++K   DL N   +   N
Sbjct: 401 TKINKLKENNWDSYINDLEKQINDL---QIDKSEEFHVIQNQLDKL--DLENYQLKNQLN 455

Query: 786 TLD------------VIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNN 929
           TLD             IK N   NL+ H   +   L+      ++      +     V N
Sbjct: 456 TLDNQKLILSQYESNFIKFNQ--NLLLHLDSIFNILQKILQESSIAQFDRKMKSIKSVPN 513

Query: 930 ALNGLDIVNDNLFKLQNFLQQA 995
           AL  L+++   L  L  F++ A
Sbjct: 514 ALKNLNLIQPKLESLYTFIETA 535
>sp|Q93Y94|RPO1_NICSY DNA-directed RNA polymerase 1, mitochondrial precursor (T7
           bacteriophage-type single subunit RNA polymerase 1)
           (NsRpoT-A)
          Length = 1002

 Score = 33.9 bits (76), Expect = 1.0
 Identities = 31/126 (24%), Positives = 53/126 (42%), Gaps = 7/126 (5%)
 Frame = +3

Query: 642 RLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFN 821
           R Y  A+E   A+   S E + +I  LIEE+NK  E L  ++  +   T+  +     +N
Sbjct: 112 RSYGSAAE---AIASTSEEDIDEIQELIEEMNKENEALKTNLQPKQPKTIGGMGVGK-YN 167

Query: 822 LINHSQIVSE-------ALRYSKSLKNVVSSSTSVTLEYMVNNALNGLDIVNDNLFKLQN 980
           L+   QI  E       A  Y + L ++     +  L YM +  L   + + D +   Q 
Sbjct: 168 LLRRRQIKVETEAWEEAAKEYQELLMDMCEQKLAPNLPYMKSLFLGWFEPLRDAIAAEQK 227

Query: 981 FLQQAR 998
              + +
Sbjct: 228 LCDEGK 233
>sp|Q27713|DRTS_PLABA Bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS)
            [Includes: Dihydrofolate reductase ; Thymidylate synthase
            ]
          Length = 587

 Score = 33.9 bits (76), Expect = 1.0
 Identities = 40/179 (22%), Positives = 78/179 (43%), Gaps = 27/179 (15%)
 Frame = +3

Query: 585  YSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVL----IEEINKRRED 752
            Y N +   ++K    K  ++   + + ++N  + +S   L  I V+     E I K+ + 
Sbjct: 61   YINENNYIRLKWKRDKYMEKHNLKNNVELNTNIISSTNNLQNIVVMGKKSWESIPKKFKP 120

Query: 753  LHNSVAQEYSNTL---DVIKKNNPFN----LINHSQIVSEALRYSKSLKNVVSSSTSVTL 911
            L N +    S TL   D++ +NN  N    +I     +   L+ +K  K  +   +SV  
Sbjct: 121  LQNRINIILSRTLKKEDIVNENNNENNNVIIIKSVDDLFPILKCTKYYKCFIIGGSSVYK 180

Query: 912  EYM------------VNNALNGLDI----VNDNLFKLQNFLQQARPESVTGELLIHTST 1040
            E++            +NN+ N  D+    +N+NLFK+ +        + T + +I++ T
Sbjct: 181  EFLDRNLIKKIYFTRINNSYN-CDVLFPEINENLFKITSISDVYYSNNTTLDFIIYSKT 238
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 128,622,138
Number of Sequences: 369166
Number of extensions: 2461845
Number of successful extensions: 7976
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7506
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7963
length of database: 68,354,980
effective HSP length: 113
effective length of database: 47,479,925
effective search space used: 14908696450
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00620

  1. Dr_sW_016_F22
  2. Dr_sW_013_O14
  3. Dr_sW_019_D16
  4. Dr_sW_001_K02
  5. Dr_sW_018_F05
  6. Dr_sW_018_F17
  7. Dr_sW_012_N18