Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_K02
(830 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage... 37 0.051
sp|Q6FS63|MAD1_CANGA Spindle assembly checkpoint component ... 35 0.19
sp|O67358|TIG_AQUAE Trigger factor (TF) 35 0.25
sp|P39723|YAE7_YEAST Hypothetical 72.1 kDa protein in ACS1-... 34 0.43
sp|Q93Y94|RPO1_NICSY DNA-directed RNA polymerase 1, mitocho... 34 0.56
sp|Q27713|DRTS_PLABA Bifunctional dihydrofolate reductase-t... 34 0.56
sp|P36158|YK58_YEAST Hypothetical 68.3 kDa protein in SIS2-... 33 0.96
sp|P77338|KEFA_ECOLI Potassium efflux system kefA (Protein ... 33 1.3
sp|P33110|CRD1_ANSAN Delta crystallin (Argininosuccinate ly... 32 1.6
sp|O15736|TIPD_DICDI Protein tipD 32 1.6
>sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog)
Length = 8545
Score = 37.4 bits (85), Expect = 0.051
Identities = 25/99 (25%), Positives = 51/99 (51%), Gaps = 1/99 (1%)
Frame = +2
Query: 188 QRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPF 367
+R Y+ +++NA + A +E L + +L E+++ +ED++N S +D + +
Sbjct: 8289 ERQYEDTMDKLNAEITAEVELLRTLDILSNELSQCKEDINNP-----SVDVDELSRATML 8343
Query: 368 N-LINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNNA 481
N I H + + S+ + V SSTS+ L+ ++ A
Sbjct: 8344 NDAIAHLENQKVVVARSEKDRKFVESSTSIDLDQLLAEA 8382
>sp|Q6FS63|MAD1_CANGA Spindle assembly checkpoint component MAD1 (Mitotic arrest
deficient protein 1)
Length = 657
Score = 35.4 bits (80), Expect = 0.19
Identities = 44/202 (21%), Positives = 78/202 (38%)
Frame = +2
Query: 53 STNYVLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALL 232
STN + D LLD Y + E D + + R G ++ R + IN
Sbjct: 382 STNRNDAEYDSLLDNYKQETENLTNELKRLNDSLIEQNRNQGTEMKKRKLRNGDHININY 441
Query: 233 KASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALRY 412
L +L Q ++ +E+ N++ V ++ LD ++K P N+ Q S L+
Sbjct: 442 SQRLNELQQNNLSLEKDNEKL----RLVVEKLEGKLDDLRKTKPKNIRILQQRDSPLLK- 496
Query: 413 SKSLKNVVSSSTSVTLEYMVNNALNGLDIVNDNLFKLQNFLQQARPESVTGELLIHTSTA 592
L+++ L N NL + + + R ES L ++
Sbjct: 497 ---------------LQFVRRKENELLREENQNLLTMIENITENRNESFDKIPLTSYNSL 541
Query: 593 VTEIRIALDSFIYHMKRYSRKK 658
+ I++D+ K++SR K
Sbjct: 542 KYQYEISMDTNKKQEKKFSRLK 563
>sp|O67358|TIG_AQUAE Trigger factor (TF)
Length = 478
Score = 35.0 bits (79), Expect = 0.25
Identities = 33/130 (25%), Positives = 61/130 (46%), Gaps = 5/130 (3%)
Frame = +2
Query: 65 VLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASL 244
V DK E+ D +P E + + +++ L T+ K+ E++ + +A++
Sbjct: 321 VADKLVEIHDIPVPQTLLRRELSFLVDRRLRELQALGIDTRYVDIKKIVEEVQPIAEANI 380
Query: 245 EKLHQIHVLIEE--INKRREDL---HNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALR 409
+ + +E I ED+ + +A++Y T+D IKK F N Q+V E R
Sbjct: 381 KLRFILDKYAQEKGIEPTGEDIEAQYKELAEQYGTTVDEIKKY--FKENNLEQVVYEDAR 438
Query: 410 YSKSLKNVVS 439
K+LK ++S
Sbjct: 439 RKKALKEIIS 448
>sp|P39723|YAE7_YEAST Hypothetical 72.1 kDa protein in ACS1-GCV3 intergenic region
Length = 622
Score = 34.3 bits (77), Expect = 0.43
Identities = 34/142 (23%), Positives = 56/142 (39%), Gaps = 12/142 (8%)
Frame = +2
Query: 155 TKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSN 334
TK+ +L +QIN L ++K + HV+ +++K DL N + N
Sbjct: 401 TKINKLKENNWDSYINDLEKQINDL---QIDKSEEFHVIQNQLDKL--DLENYQLKNQLN 455
Query: 335 TLD------------VIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNN 478
TLD IK N NL+ H + L+ ++ + V N
Sbjct: 456 TLDNQKLILSQYESNFIKFNQ--NLLLHLDSIFNILQKILQESSIAQFDRKMKSIKSVPN 513
Query: 479 ALNGLDIVNDNLFKLQNFLQQA 544
AL L+++ L L F++ A
Sbjct: 514 ALKNLNLIQPKLESLYTFIETA 535
>sp|Q93Y94|RPO1_NICSY DNA-directed RNA polymerase 1, mitochondrial precursor (T7
bacteriophage-type single subunit RNA polymerase 1)
(NsRpoT-A)
Length = 1002
Score = 33.9 bits (76), Expect = 0.56
Identities = 31/126 (24%), Positives = 53/126 (42%), Gaps = 7/126 (5%)
Frame = +2
Query: 191 RLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFN 370
R Y A+E A+ S E + +I LIEE+NK E L ++ + T+ + +N
Sbjct: 112 RSYGSAAE---AIASTSEEDIDEIQELIEEMNKENEALKTNLQPKQPKTIGGMGVGK-YN 167
Query: 371 LINHSQIVSE-------ALRYSKSLKNVVSSSTSVTLEYMVNNALNGLDIVNDNLFKLQN 529
L+ QI E A Y + L ++ + L YM + L + + D + Q
Sbjct: 168 LLRRRQIKVETEAWEEAAKEYQELLMDMCEQKLAPNLPYMKSLFLGWFEPLRDAIAAEQK 227
Query: 530 FLQQAR 547
+ +
Sbjct: 228 LCDEGK 233
>sp|Q27713|DRTS_PLABA Bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS)
[Includes: Dihydrofolate reductase ; Thymidylate
synthase ]
Length = 587
Score = 33.9 bits (76), Expect = 0.56
Identities = 40/179 (22%), Positives = 78/179 (43%), Gaps = 27/179 (15%)
Frame = +2
Query: 134 YSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVL----IEEINKRRED 301
Y N + ++K K ++ + + ++N + +S L I V+ E I K+ +
Sbjct: 61 YINENNYIRLKWKRDKYMEKHNLKNNVELNTNIISSTNNLQNIVVMGKKSWESIPKKFKP 120
Query: 302 LHNSVAQEYSNTL---DVIKKNNPFN----LINHSQIVSEALRYSKSLKNVVSSSTSVTL 460
L N + S TL D++ +NN N +I + L+ +K K + +SV
Sbjct: 121 LQNRINIILSRTLKKEDIVNENNNENNNVIIIKSVDDLFPILKCTKYYKCFIIGGSSVYK 180
Query: 461 EYM------------VNNALNGLDI----VNDNLFKLQNFLQQARPESVTGELLIHTST 589
E++ +NN+ N D+ +N+NLFK+ + + T + +I++ T
Sbjct: 181 EFLDRNLIKKIYFTRINNSYN-CDVLFPEINENLFKITSISDVYYSNNTTLDFIIYSKT 238
>sp|P36158|YK58_YEAST Hypothetical 68.3 kDa protein in SIS2-MTD1 intergenic region
Length = 585
Score = 33.1 bits (74), Expect = 0.96
Identities = 54/236 (22%), Positives = 99/236 (41%), Gaps = 26/236 (11%)
Frame = +2
Query: 53 STNYVLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINA-- 226
ST+ + D L+ +++P + T LS + Y+ AS +I A
Sbjct: 324 STDNTFNNNDPLIQEWIPKSK-------------TLFTSLSFGSSAPTYQEASTEIQACH 370
Query: 227 ----LLKASLEKL--HQIHVLIEEINKRREDLHNSVAQEYSN-----TLDVIKKNNPFNL 373
+ K ++L H + +++E K NSV E++ +LD + + N
Sbjct: 371 DWVSISKEQWKQLLYHVLQYIVDEAVKV-----NSVINEFTECLKQISLDEVIRANSELF 425
Query: 374 INHSQIVSEALRYSKS------LKNVVSSSTSV----TLEYMVNNALNG---LDIVNDNL 514
+ S++ L+ K LK ++ +V + E ++N L L I+ +L
Sbjct: 426 LKFSKLNESFLKKFKGASRQDILKLIILFDENVRFCESFESILNQRLKLGKILSIIEVDL 485
Query: 515 FKLQNFLQQARPESVTGELLIHTSTAVTEIRIALDSFIYHMKRYSRKKFHGEEITE 682
K +NFL + P + +++ +IR A D + +KRY+R K E+I E
Sbjct: 486 DKKKNFLDKLSPGNN------NSNNEDLKIRTAEDEYRIVLKRYNRVKQSWEKIME 535
>sp|P77338|KEFA_ECOLI Potassium efflux system kefA (Protein aefA)
Length = 1120
Score = 32.7 bits (73), Expect = 1.3
Identities = 38/168 (22%), Positives = 72/168 (42%)
Frame = +2
Query: 140 NADIVTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVA 319
NA+I + K L G T L +Q + + S HQ+ +L E +N +R L A
Sbjct: 212 NAEIDQQRKSLEGNTV--LQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTA 269
Query: 320 QEYSNTLDVIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNNALNGLDI 499
QE + + + I + +V + L ++ L + ++T NG +
Sbjct: 270 QEAVSPDEAAR-------IQANPLVKQELEINQQLSQRLITATE-----------NGNQL 311
Query: 500 VNDNLFKLQNFLQQARPESVTGELLIHTSTAVTEIRIALDSFIYHMKR 643
+ N+ K++N+L++A + E I AV + + L +Y ++
Sbjct: 312 MQQNI-KVKNWLERA----LQSERNIKEQIAVLKGSLLLSRILYQQQQ 354
>sp|P33110|CRD1_ANSAN Delta crystallin (Argininosuccinate lyase)
Length = 466
Score = 32.3 bits (72), Expect = 1.6
Identities = 36/165 (21%), Positives = 69/165 (41%), Gaps = 18/165 (10%)
Frame = +2
Query: 140 NADIVTKVKRLSGKTQQRL----------YKRASEQINALLKASLEK-LHQIHVLIEE-- 280
+ D + ++ S T+QRL Y +A E+ L K LEK L + + EE
Sbjct: 16 STDPIMQMLSTSMSTEQRLSEVDIQASIAYAKALEKAGILTKTELEKILSGLEKISEEWS 75
Query: 281 -----INKRREDLHNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSS 445
+ + ED+H + + + I + Q+V++ + K+ +V+S+
Sbjct: 76 KGVFVVTQSDEDIHTANERRLKELIGDIAGKLNTGRSRNEQVVTDLKLFMKNSLSVISTH 135
Query: 446 TSVTLEYMVNNALNGLDIVNDNLFKLQNFLQQARPESVTGELLIH 580
++ +V A +D++ LQ+A+P + LL H
Sbjct: 136 LLQLIKTLVERAAIEIDVILPG----YTHLQKAQPIRWSQFLLSH 176
>sp|O15736|TIPD_DICDI Protein tipD
Length = 612
Score = 32.3 bits (72), Expect = 1.6
Identities = 40/176 (22%), Positives = 76/176 (43%), Gaps = 5/176 (2%)
Frame = +2
Query: 179 KTQQRLYKRASEQINALLKASLEKLHQIHVLIEEIN----KRREDLHNSVAQEYSNTLDV 346
KTQQ+ +S+ ++ +S ++ + IEE+ K +EDL NS + N +
Sbjct: 99 KTQQQPPSGSSKMDSSSSSSSSNRVSGMGSTIEEMEQKLFKLQEDLTNSYKRNADNASSI 158
Query: 347 IKKNNPFNLINHSQIVSEALRYSKSLKNVVSSSTSV-TLEYMVNNALNGLDIVNDNLFKL 523
+ N+ N ++++S+ + + + S+ LE +V N I+ D L L
Sbjct: 159 LLLNDK-NKDLQNELMSKEIEIERIRSTIQQDLDSIKRLEMVVIEKENVSQIIRDELSSL 217
Query: 524 QNFLQQARPESVTGELLIHTSTAVTEIRIALDSFIYHMKRYSRKKFHGEEITEMHE 691
Q +H + V ++ S + +R+ RKK EE ++M+E
Sbjct: 218 QT-------------EFLHNESKVVKLEQENSSLV---ERWLRKK--NEEASKMNE 255
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,072,512
Number of Sequences: 369166
Number of extensions: 1733265
Number of successful extensions: 6195
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5890
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6182
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8052550455
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)