Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_019_D16
(897 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage... 37 0.057
sp|O67358|TIG_AQUAE Trigger factor (TF) 35 0.28
sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-r... 34 0.48
sp|P39723|YAE7_YEAST Hypothetical 72.1 kDa protein in ACS1-... 34 0.48
sp|Q93Y94|RPO1_NICSY DNA-directed RNA polymerase 1, mitocho... 34 0.63
sp|Q27713|DRTS_PLABA Bifunctional dihydrofolate reductase-t... 34 0.63
sp|Q6FS63|MAD1_CANGA Spindle assembly checkpoint component ... 33 0.83
sp|P33110|CRD1_ANSAN Delta crystallin (Argininosuccinate ly... 32 1.8
sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546 32 1.8
sp|P02521|CRD1_CHICK Delta crystallin I 32 3.1
>sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog)
Length = 8545
Score = 37.4 bits (85), Expect = 0.057
Identities = 25/99 (25%), Positives = 51/99 (51%), Gaps = 1/99 (1%)
Frame = +2
Query: 488 QRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPF 667
+R Y+ +++NA + A +E L + +L E+++ +ED++N S +D + +
Sbjct: 8289 ERQYEDTMDKLNAEITAEVELLRTLDILSNELSQCKEDINNP-----SVDVDELSRATML 8343
Query: 668 N-LINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNNA 781
N I H + + S+ + V SSTS+ L+ ++ A
Sbjct: 8344 NDAIAHLENQKVVVARSEKDRKFVESSTSIDLDQLLAEA 8382
>sp|O67358|TIG_AQUAE Trigger factor (TF)
Length = 478
Score = 35.0 bits (79), Expect = 0.28
Identities = 33/130 (25%), Positives = 61/130 (46%), Gaps = 5/130 (3%)
Frame = +2
Query: 365 VLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASL 544
V DK E+ D +P E + + +++ L T+ K+ E++ + +A++
Sbjct: 321 VADKLVEIHDIPVPQTLLRRELSFLVDRRLRELQALGIDTRYVDIKKIVEEVQPIAEANI 380
Query: 545 EKLHQIHVLIEE--INKRREDL---HNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALR 709
+ + +E I ED+ + +A++Y T+D IKK F N Q+V E R
Sbjct: 381 KLRFILDKYAQEKGIEPTGEDIEAQYKELAEQYGTTVDEIKKY--FKENNLEQVVYEDAR 438
Query: 710 YSKSLKNVVS 739
K+LK ++S
Sbjct: 439 RKKALKEIIS 448
>sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 450
Score = 34.3 bits (77), Expect = 0.48
Identities = 46/221 (20%), Positives = 86/221 (38%), Gaps = 25/221 (11%)
Frame = +2
Query: 26 AEKTLKAVKDTAEPIAVR-------QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXX 184
AEK +K + A A+ Q+ + LDR E+K PI+ +
Sbjct: 50 AEKGMKTITSVAVTSALPIIQKLEPQIAVANTYACKGLDRIEEKLPILNQPTNQVVANAK 109
Query: 185 XXY---HDSVEST----KKTLMWPVTKTLDTVSKTSDFAXXXXXXXXXXXMKIVLPATYL 343
D+V +T K ++ +T +D + + VL + +
Sbjct: 110 GAMTGAKDAVTTTVTGAKDSVASTITGVVDRTKGAVTGSVEKTKSVVSGSINTVLRSRVM 169
Query: 344 TNVSTNY--VLDKTDELLDKYLP---DNNQCNEEPYSNADIVTK------VKRLSGKTQQ 490
+S+ L K++ L+D+YLP D + + D+V K + LS K +
Sbjct: 170 QLMSSGVENALTKSELLVDQYLPLTKDELEKEAKKVEGFDMVQKPSYYVRLGSLSTKLRS 229
Query: 491 RLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNS 613
R Y++A ++ + E + Q+H R+++HN+
Sbjct: 230 RAYQQALCRVEEAKRKGQETISQLHSAFNLSELARKNVHNA 270
>sp|P39723|YAE7_YEAST Hypothetical 72.1 kDa protein in ACS1-GCV3 intergenic region
Length = 622
Score = 34.3 bits (77), Expect = 0.48
Identities = 34/142 (23%), Positives = 56/142 (39%), Gaps = 12/142 (8%)
Frame = +2
Query: 455 TKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSN 634
TK+ +L +QIN L ++K + HV+ +++K DL N + N
Sbjct: 401 TKINKLKENNWDSYINDLEKQINDL---QIDKSEEFHVIQNQLDKL--DLENYQLKNQLN 455
Query: 635 TLD------------VIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNN 778
TLD IK N NL+ H + L+ ++ + V N
Sbjct: 456 TLDNQKLILSQYESNFIKFNQ--NLLLHLDSIFNILQKILQESSIAQFDRKMKSIKSVPN 513
Query: 779 ALNGLDIVNDNLFKLQNFLQQA 844
AL L+++ L L F++ A
Sbjct: 514 ALKNLNLIQPKLESLYTFIETA 535
>sp|Q93Y94|RPO1_NICSY DNA-directed RNA polymerase 1, mitochondrial precursor (T7
bacteriophage-type single subunit RNA polymerase 1)
(NsRpoT-A)
Length = 1002
Score = 33.9 bits (76), Expect = 0.63
Identities = 31/126 (24%), Positives = 53/126 (42%), Gaps = 7/126 (5%)
Frame = +2
Query: 491 RLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFN 670
R Y A+E A+ S E + +I LIEE+NK E L ++ + T+ + +N
Sbjct: 112 RSYGSAAE---AIASTSEEDIDEIQELIEEMNKENEALKTNLQPKQPKTIGGMGVGK-YN 167
Query: 671 LINHSQIVSE-------ALRYSKSLKNVVSSSTSVTLEYMVNNALNGLDIVNDNLFKLQN 829
L+ QI E A Y + L ++ + L YM + L + + D + Q
Sbjct: 168 LLRRRQIKVETEAWEEAAKEYQELLMDMCEQKLAPNLPYMKSLFLGWFEPLRDAIAAEQK 227
Query: 830 FLQQAR 847
+ +
Sbjct: 228 LCDEGK 233
>sp|Q27713|DRTS_PLABA Bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS)
[Includes: Dihydrofolate reductase ; Thymidylate
synthase ]
Length = 587
Score = 33.9 bits (76), Expect = 0.63
Identities = 40/179 (22%), Positives = 78/179 (43%), Gaps = 27/179 (15%)
Frame = +2
Query: 434 YSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVL----IEEINKRRED 601
Y N + ++K K ++ + + ++N + +S L I V+ E I K+ +
Sbjct: 61 YINENNYIRLKWKRDKYMEKHNLKNNVELNTNIISSTNNLQNIVVMGKKSWESIPKKFKP 120
Query: 602 LHNSVAQEYSNTL---DVIKKNNPFN----LINHSQIVSEALRYSKSLKNVVSSSTSVTL 760
L N + S TL D++ +NN N +I + L+ +K K + +SV
Sbjct: 121 LQNRINIILSRTLKKEDIVNENNNENNNVIIIKSVDDLFPILKCTKYYKCFIIGGSSVYK 180
Query: 761 EYM------------VNNALNGLDI----VNDNLFKLQNFLQQARPESVTGELLIHTST 889
E++ +NN+ N D+ +N+NLFK+ + + T + +I++ T
Sbjct: 181 EFLDRNLIKKIYFTRINNSYN-CDVLFPEINENLFKITSISDVYYSNNTTLDFIIYSKT 238
>sp|Q6FS63|MAD1_CANGA Spindle assembly checkpoint component MAD1 (Mitotic arrest
deficient protein 1)
Length = 657
Score = 33.5 bits (75), Expect = 0.83
Identities = 26/107 (24%), Positives = 45/107 (42%)
Frame = +2
Query: 353 STNYVLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALL 532
STN + D LLD Y + E D + + R G ++ R + IN
Sbjct: 382 STNRNDAEYDSLLDNYKQETENLTNELKRLNDSLIEQNRNQGTEMKKRKLRNGDHININY 441
Query: 533 KASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFNL 673
L +L Q ++ +E+ N++ V ++ LD ++K P N+
Sbjct: 442 SQRLNELQQNNLSLEKDNEKL----RLVVEKLEGKLDDLRKTKPKNI 484
>sp|P33110|CRD1_ANSAN Delta crystallin (Argininosuccinate lyase)
Length = 466
Score = 32.3 bits (72), Expect = 1.8
Identities = 36/165 (21%), Positives = 69/165 (41%), Gaps = 18/165 (10%)
Frame = +2
Query: 440 NADIVTKVKRLSGKTQQRL----------YKRASEQINALLKASLEK-LHQIHVLIEE-- 580
+ D + ++ S T+QRL Y +A E+ L K LEK L + + EE
Sbjct: 16 STDPIMQMLSTSMSTEQRLSEVDIQASIAYAKALEKAGILTKTELEKILSGLEKISEEWS 75
Query: 581 -----INKRREDLHNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSS 745
+ + ED+H + + + I + Q+V++ + K+ +V+S+
Sbjct: 76 KGVFVVTQSDEDIHTANERRLKELIGDIAGKLNTGRSRNEQVVTDLKLFMKNSLSVISTH 135
Query: 746 TSVTLEYMVNNALNGLDIVNDNLFKLQNFLQQARPESVTGELLIH 880
++ +V A +D++ LQ+A+P + LL H
Sbjct: 136 LLQLIKTLVERAAIEIDVILPG----YTHLQKAQPIRWSQFLLSH 176
>sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546
Length = 62
Score = 32.3 bits (72), Expect = 1.8
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +2
Query: 452 VTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINK 589
+ VK L G++ ++L +E NA+ K + IHV+IEE+ K
Sbjct: 3 IINVKLLEGRSDEQLKNLVTEVTNAVEKTTGANREAIHVIIEEMQK 48
>sp|P02521|CRD1_CHICK Delta crystallin I
Length = 466
Score = 31.6 bits (70), Expect = 3.1
Identities = 34/150 (22%), Positives = 62/150 (41%), Gaps = 18/150 (12%)
Frame = +2
Query: 440 NADIVTKVKRLSGKTQQRL----------YKRASEQINALLKASLEK-LHQIHVLIEEIN 586
+ D + ++ S T+QRL Y +A E+ + L K LEK L + + EE +
Sbjct: 16 STDPIMEILSSSISTEQRLTEVDIQASMAYAKALEKASILTKTELEKILSGLEKISEESS 75
Query: 587 K-------RREDLHNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSS 745
K ED+ ++ + + I + Q+V++ KS +V+S+
Sbjct: 76 KGVLVMTQSDEDIQTAIERRLKELIGDIAGKLQTGRSRNEQVVTDLKLLLKSSISVISTH 135
Query: 746 TSVTLEYMVNNALNGLDIVNDNLFKLQNFL 835
++ +V A +DI+ LQ L
Sbjct: 136 LLQLIKTLVERAAIEIDIIMPGYTHLQKAL 165
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,904,504
Number of Sequences: 369166
Number of extensions: 1632680
Number of successful extensions: 5703
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5434
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5697
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9030416440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)