Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_F22
(876 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9VCI3|LSD1_DROME Lipid storage droplets surface binding... 55 3e-07
sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding... 49 2e-05
sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-r... 47 7e-05
sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-r... 44 6e-04
sp|Q6FS63|MAD1_CANGA Spindle assembly checkpoint component ... 34 0.61
sp|P45385|IGA3_HAEIN Immunoglobulin A1 protease precursor (... 32 1.8
sp|P42782|IGA1_HAEIN Immunoglobulin A1 protease precursor (... 32 1.8
sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546 32 1.8
sp|Q9UTR7|MCP3_SCHPO Meiotic coiled-coil protein 3 32 3.0
sp|O60664|M6PBP_HUMAN Mannose-6-phosphate receptor binding ... 31 4.0
>sp|Q9VCI3|LSD1_DROME Lipid storage droplets surface binding protein 1
Length = 431
Score = 55.1 bits (131), Expect = 3e-07
Identities = 64/269 (23%), Positives = 109/269 (40%), Gaps = 35/269 (13%)
Frame = +3
Query: 33 ASSHSDNHFKSLDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTA 212
A+S S H +++DR+ P+V S++ V + Y +K++N + AE T+ A +T
Sbjct: 4 ATSGSGLHLEAIDRIGSIPLVESSVKRVETIYDKVKNNNRLFSWYFETAEATISAAYETI 63
Query: 213 EPIAVR----QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLM 380
+P AV+ + +DN + LD E++ P++ Y ++ E L+
Sbjct: 64 QP-AVKLFEPSIQRLDNVMCKSLDILEQRIPLV-------YLPPEMMYWNTKEYMSDHLV 115
Query: 381 WPVTKTLDTV---------SKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNYVLDKTD 533
PV K D+V S + +A K V YL + T+ D+TD
Sbjct: 116 RPVLKRADSVKQIGNAVLESPLTTYAAERIDGAFTVGDKFV--DKYLVPIQTDQ--DQTD 171
Query: 534 ------ELLDKYLP----------------DNNQCNEEPYSNADIVTKVKRLSGKTQQRL 647
L D + P D+N+ + + +R S K ++RL
Sbjct: 172 VKKLVSALNDNFSPIRQQLNRMFGHKSPQEDDNEAVPDERGAIKAIHHGQRFSRKLKRRL 231
Query: 648 YKRASEQINALLKASLEKLHQIHVLIEEI 734
+R + AL K S E +H + E I
Sbjct: 232 TQRTIAEARALKKQSKEAIHVLFYAAELI 260
>sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding protein 2 (Lsd2)
Length = 352
Score = 48.9 bits (115), Expect = 2e-05
Identities = 59/263 (22%), Positives = 95/263 (36%), Gaps = 44/263 (16%)
Frame = +3
Query: 54 HFKSLDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAV-- 227
H +SL+R+ P+VN + + Y +K N + + LT AE + TA P
Sbjct: 36 HLESLERIIKLPVVNAAWDKSQDVYGKVKGKNRVFEWALTAAEDCVTRAVTTAAPFVTKL 95
Query: 228 -RQLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWPVTKTLD 404
R + ++D ++ +D+ E K PII + D V+ + ++
Sbjct: 96 DRPIAYVDQTLVKGIDKLEVKAPIIKDTPQEIYNQAKSKVIDVVQPHLERVVKFKAAGQQ 155
Query: 405 TVSKTSDFAXXXXXXXXXXXMKIVLPATY----LTNVSTNYVLDKTDELLDKYLP----- 557
+ D A VL Y + V T L + LL+ Y P
Sbjct: 156 KAASLKDLAWQKANE--------VLATQYGSLAVNGVDTTTAL--AERLLEYYFPKCESD 205
Query: 558 ---DNNQ-------------------CNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQI 671
DN+ +E+P + V V RLS K +R+Y+ S QI
Sbjct: 206 VEEDNDDKQNAVVQNGKSSENDMPVPASEDPVLHT--VQTVGRLSNKISRRVYRNVSRQI 263
Query: 672 NALLKASLE----------KLHQ 710
+ K ++ KLHQ
Sbjct: 264 KQVQKGNINDYLSSLIAALKLHQ 286
>sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 425
Score = 47.0 bits (110), Expect = 7e-05
Identities = 52/254 (20%), Positives = 94/254 (37%), Gaps = 23/254 (9%)
Frame = +3
Query: 72 RVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAV-------R 230
RV + P+V+ + + V S Y K L + AEK +K V A A+
Sbjct: 15 RVANLPLVSSTYDLVSSAYVSTKDQYPYLRSVCEMAEKGVKTVTSAAMTSALPIIQKLEP 74
Query: 231 QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWPVTKTLDTV 410
Q+ + LDR E++ PI+ + + + T+ TV
Sbjct: 75 QIAVANTYACKGLDRMEERLPILNQPTSEIVASARGAVTGAKDVVTTTMAGAKDSVASTV 134
Query: 411 SKTSDFAXXXXXXXXXXXMKI-------VLPATYLTNVSTNYVLDKTDELLDKYLP---D 560
S D + VL N + + K++ L+D+Y P +
Sbjct: 135 SGVVDKTKGAVTGSVERTKSVVNGSINTVLGMVQFMNSGVDNAITKSEMLVDQYFPLTQE 194
Query: 561 NNQCNEEPYSNADIVTK------VKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVL 722
+ + D+V K ++ LS K R Y +A ++ + S E + Q+H
Sbjct: 195 ELEMEAKKVEGFDMVQKPSNYERLESLSTKLCSRAYHQALSRVKEAKQKSQETISQLHST 254
Query: 723 IEEINKRREDLHNS 764
+ I R+++H++
Sbjct: 255 VHLIEFARKNMHSA 268
>sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 450
Score = 43.9 bits (102), Expect = 6e-04
Identities = 57/270 (21%), Positives = 106/270 (39%), Gaps = 25/270 (9%)
Frame = +3
Query: 30 VASSHSDNHFKSLDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDT 209
+AS + + RV + P+V+ + + V S Y K L + AEK +K +
Sbjct: 1 MASVAVEPQLSVVTRVANLPLVSSTYDLVSSAYISRKDQYPYLKSLCEMAEKGMKTITSV 60
Query: 210 AEPIAVR-------QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXY---HDSVE 359
A A+ Q+ + LDR E+K PI+ + D+V
Sbjct: 61 AVTSALPIIQKLEPQIAVANTYACKGLDRIEEKLPILNQPTNQVVANAKGAMTGAKDAVT 120
Query: 360 ST----KKTLMWPVTKTLDTVSKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNY--VL 521
+T K ++ +T +D + + VL + + +S+ L
Sbjct: 121 TTVTGAKDSVASTITGVVDRTKGAVTGSVEKTKSVVSGSINTVLRSRVMQLMSSGVENAL 180
Query: 522 DKTDELLDKYLP---DNNQCNEEPYSNADIVTK------VKRLSGKTQQRLYKRASEQIN 674
K++ L+D+YLP D + + D+V K + LS K + R Y++A ++
Sbjct: 181 TKSELLVDQYLPLTKDELEKEAKKVEGFDMVQKPSYYVRLGSLSTKLRSRAYQQALCRVE 240
Query: 675 ALLKASLEKLHQIHVLIEEINKRREDLHNS 764
+ E + Q+H R+++HN+
Sbjct: 241 EAKRKGQETISQLHSAFNLSELARKNVHNA 270
>sp|Q6FS63|MAD1_CANGA Spindle assembly checkpoint component MAD1 (Mitotic arrest
deficient protein 1)
Length = 657
Score = 33.9 bits (76), Expect = 0.61
Identities = 31/123 (25%), Positives = 53/123 (43%)
Frame = +3
Query: 504 STNYVLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALL 683
STN + D LLD Y + E D + + R G ++ R + IN
Sbjct: 382 STNRNDAEYDSLLDNYKQETENLTNELKRLNDSLIEQNRNQGTEMKKRKLRNGDHININY 441
Query: 684 KASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFNLYQSLSNRF*SSPL 863
L +L Q ++ +E+ N++ V ++ LD ++K P N+ + L R SPL
Sbjct: 442 SQRLNELQQNNLSLEKDNEKL----RLVVEKLEGKLDDLRKTKPKNI-RILQQR--DSPL 494
Query: 864 FEI 872
++
Sbjct: 495 LKL 497
>sp|P45385|IGA3_HAEIN Immunoglobulin A1 protease precursor (IGA1 protease)
Length = 1545
Score = 32.3 bits (72), Expect = 1.8
Identities = 33/119 (27%), Positives = 52/119 (43%), Gaps = 6/119 (5%)
Frame = +3
Query: 507 TNYVLDKTDELLDKYL----PDNNQCNEEPYSNADIVTKVKRLSGKTQQR--LYKRASEQ 668
T YV TD+L DK L P N + N +A+ V L G Q R R +E
Sbjct: 796 TGYVTCTTDKLSDKALNSFNPTNLRGNVNLTESANFVLGKANLFGTIQSRGNSQVRLTEN 855
Query: 669 INALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFNLYQSLSNR 845
+ L + + +HQ+ + I+ D N+V + + T++ + N F LSN+
Sbjct: 856 SHWHLTGNSD-VHQLDLANGHIHLNSADNSNNVTKYNTLTVNSLSGNGSFYYLTDLSNK 913
>sp|P42782|IGA1_HAEIN Immunoglobulin A1 protease precursor (IGA1 protease)
Length = 1541
Score = 32.3 bits (72), Expect = 1.8
Identities = 33/119 (27%), Positives = 52/119 (43%), Gaps = 6/119 (5%)
Frame = +3
Query: 507 TNYVLDKTDELLDKYL----PDNNQCNEEPYSNADIVTKVKRLSGKTQQR--LYKRASEQ 668
T YV TD+L DK L P N + N +A+ V L G Q R R +E
Sbjct: 792 TGYVTCTTDKLSDKALNSFNPTNLRGNVNLTESANFVLGKANLFGTIQSRGNSQVRLTEN 851
Query: 669 INALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFNLYQSLSNR 845
+ L + + +HQ+ + I+ D N+V + + T++ + N F LSN+
Sbjct: 852 SHWHLTGNSD-VHQLDLANGHIHLNSADNSNNVTKYNTLTVNSLSGNGSFYYLTDLSNK 909
>sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546
Length = 62
Score = 32.3 bits (72), Expect = 1.8
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +3
Query: 603 VTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINK 740
+ VK L G++ ++L +E NA+ K + IHV+IEE+ K
Sbjct: 3 IINVKLLEGRSDEQLKNLVTEVTNAVEKTTGANREAIHVIIEEMQK 48
>sp|Q9UTR7|MCP3_SCHPO Meiotic coiled-coil protein 3
Length = 952
Score = 31.6 bits (70), Expect = 3.0
Identities = 25/74 (33%), Positives = 33/74 (44%), Gaps = 5/74 (6%)
Frame = +2
Query: 632 NSAKTL*KSFRTNQCTSEGEFRKIAPN-----SCSNRRD*QEKRRPAQFCCSGILEYFRC 796
NS K L KS NQ T+ +PN S +D Q RR F CS + C
Sbjct: 43 NSFKLLVKSEGNNQSTTADVTLTKSPNAYHASSAREEKDLQVSRRDTCFYCSRKIIKCIC 102
Query: 797 NKEKQPIQSLSITL 838
N+E I+S+ + L
Sbjct: 103 NEEHVGIESVQLKL 116
>sp|O60664|M6PBP_HUMAN Mannose-6-phosphate receptor binding protein 1 (Cargo selection
protein TIP47) (47 kDa mannose 6-phosphate
receptor-binding protein) (47 kDa MPR-binding protein)
(Placental protein 17) (PP17)
Length = 434
Score = 31.2 bits (69), Expect = 4.0
Identities = 51/245 (20%), Positives = 88/245 (35%), Gaps = 18/245 (7%)
Frame = +3
Query: 66 LDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLK----AVKDTAEPIAVR- 230
+DRV P+++ + + V + Y K S + AEK ++ A A+PI +
Sbjct: 26 VDRVASMPLISSTCDMVSAAYASTKESYPHIKTVCDAAEKGVRTLTAAAVSGAQPILSKL 85
Query: 231 --QLGFIDNAVLNQLDRFEKKCPI-------ILEXXXXXXXXXXXXYHDSVESTKKTLMW 383
Q+ LD+ E+ PI +L + V S K T+
Sbjct: 86 EPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAKDTVAT 145
Query: 384 PVTKTLD----TVSKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNYVLDKTDELLDKY 551
+++ +D V D M L L+ V T VL K++E D +
Sbjct: 146 QLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDT--VLGKSEEWADNH 203
Query: 552 LPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEE 731
L P ++A++ L G + ++ EQ + SL + + H
Sbjct: 204 L---------PLTDAELARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHS 254
Query: 732 INKRR 746
+ K R
Sbjct: 255 LGKLR 259
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,128,285
Number of Sequences: 369166
Number of extensions: 1378894
Number of successful extensions: 4545
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4379
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4541
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8694177530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)