Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_O14
(857 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9VCI3|LSD1_DROME Lipid storage droplets surface binding... 52 2e-06
sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding... 49 2e-05
sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-r... 47 7e-05
sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-r... 44 8e-04
sp|O67358|TIG_AQUAE Trigger factor (TF) 35 0.27
sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage... 34 0.60
sp|Q6FS63|MAD1_CANGA Spindle assembly checkpoint component ... 33 0.78
sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546 32 1.7
sp|Q96L93|SNX23_HUMAN Kinesin-like motor protein C20orf23 (... 32 3.0
sp|Q02331|YOL4_CAEEL Hypothetical UPF0028 protein ZK370.4 i... 32 3.0
>sp|Q9VCI3|LSD1_DROME Lipid storage droplets surface binding protein 1
Length = 431
Score = 52.4 bits (124), Expect = 2e-06
Identities = 61/262 (23%), Positives = 105/262 (40%), Gaps = 35/262 (13%)
Frame = +2
Query: 2 HFKSLDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAVR- 178
H +++DR+ P+V S++ V + Y +K++N + AE T+ A +T +P AV+
Sbjct: 11 HLEAIDRIGSIPLVESSVKRVETIYDKVKNNNRLFSWYFETAEATISAAYETIQP-AVKL 69
Query: 179 ---QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWPVTKTL 349
+ +DN + LD E++ P++ Y ++ E L+ PV K
Sbjct: 70 FEPSIQRLDNVMCKSLDILEQRIPLV-------YLPPEMMYWNTKEYMSDHLVRPVLKRA 122
Query: 350 DTV---------SKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNYVLDKTD------E 484
D+V S + +A K V YL + T+ D+TD
Sbjct: 123 DSVKQIGNAVLESPLTTYAAERIDGAFTVGDKFV--DKYLVPIQTDQ--DQTDVKKLVSA 178
Query: 485 LLDKYLP----------------DNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQ 616
L D + P D+N+ + + +R S K ++RL +R +
Sbjct: 179 LNDNFSPIRQQLNRMFGHKSPQEDDNEAVPDERGAIKAIHHGQRFSRKLKRRLTQRTIAE 238
Query: 617 INALLKASLEKLHQIHVLIEEI 682
AL K S E +H + E I
Sbjct: 239 ARALKKQSKEAIHVLFYAAELI 260
>sp|Q9VXY7|LSD2_DROME Lipid storage droplets surface binding protein 2 (Lsd2)
Length = 352
Score = 48.9 bits (115), Expect = 2e-05
Identities = 59/263 (22%), Positives = 95/263 (36%), Gaps = 44/263 (16%)
Frame = +2
Query: 2 HFKSLDRVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAV-- 175
H +SL+R+ P+VN + + Y +K N + + LT AE + TA P
Sbjct: 36 HLESLERIIKLPVVNAAWDKSQDVYGKVKGKNRVFEWALTAAEDCVTRAVTTAAPFVTKL 95
Query: 176 -RQLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWPVTKTLD 352
R + ++D ++ +D+ E K PII + D V+ + ++
Sbjct: 96 DRPIAYVDQTLVKGIDKLEVKAPIIKDTPQEIYNQAKSKVIDVVQPHLERVVKFKAAGQQ 155
Query: 353 TVSKTSDFAXXXXXXXXXXXMKIVLPATY----LTNVSTNYVLDKTDELLDKYLP----- 505
+ D A VL Y + V T L + LL+ Y P
Sbjct: 156 KAASLKDLAWQKANE--------VLATQYGSLAVNGVDTTTAL--AERLLEYYFPKCESD 205
Query: 506 ---DNNQ-------------------CNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQI 619
DN+ +E+P + V V RLS K +R+Y+ S QI
Sbjct: 206 VEEDNDDKQNAVVQNGKSSENDMPVPASEDPVLHT--VQTVGRLSNKISRRVYRNVSRQI 263
Query: 620 NALLKASLE----------KLHQ 658
+ K ++ KLHQ
Sbjct: 264 KQVQKGNINDYLSSLIAALKLHQ 286
>sp|P43883|ADFP_MOUSE Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 425
Score = 47.0 bits (110), Expect = 7e-05
Identities = 52/254 (20%), Positives = 94/254 (37%), Gaps = 23/254 (9%)
Frame = +2
Query: 20 RVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAV-------R 178
RV + P+V+ + + V S Y K L + AEK +K V A A+
Sbjct: 15 RVANLPLVSSTYDLVSSAYVSTKDQYPYLRSVCEMAEKGVKTVTSAAMTSALPIIQKLEP 74
Query: 179 QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXYHDSVESTKKTLMWPVTKTLDTV 358
Q+ + LDR E++ PI+ + + + T+ TV
Sbjct: 75 QIAVANTYACKGLDRMEERLPILNQPTSEIVASARGAVTGAKDVVTTTMAGAKDSVASTV 134
Query: 359 SKTSDFAXXXXXXXXXXXMKI-------VLPATYLTNVSTNYVLDKTDELLDKYLP---D 508
S D + VL N + + K++ L+D+Y P +
Sbjct: 135 SGVVDKTKGAVTGSVERTKSVVNGSINTVLGMVQFMNSGVDNAITKSEMLVDQYFPLTQE 194
Query: 509 NNQCNEEPYSNADIVTK------VKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVL 670
+ + D+V K ++ LS K R Y +A ++ + S E + Q+H
Sbjct: 195 ELEMEAKKVEGFDMVQKPSNYERLESLSTKLCSRAYHQALSRVKEAKQKSQETISQLHST 254
Query: 671 IEEINKRREDLHNS 712
+ I R+++H++
Sbjct: 255 VHLIEFARKNMHSA 268
>sp|Q9TUM6|ADFP_BOVIN Adipophilin (Adipose differentiation-related protein) (ADRP)
Length = 450
Score = 43.5 bits (101), Expect = 8e-04
Identities = 55/256 (21%), Positives = 101/256 (39%), Gaps = 25/256 (9%)
Frame = +2
Query: 20 RVHHYPIVNDSLEYVLSYYQWIKSSNGILDNTLTRAEKTLKAVKDTAEPIAVR------- 178
RV + P+V+ + + V S Y K L + AEK +K + A A+
Sbjct: 15 RVANLPLVSSTYDLVSSAYISRKDQYPYLKSLCEMAEKGMKTITSVAVTSALPIIQKLEP 74
Query: 179 QLGFIDNAVLNQLDRFEKKCPIILEXXXXXXXXXXXXY---HDSVEST----KKTLMWPV 337
Q+ + LDR E+K PI+ + D+V +T K ++ +
Sbjct: 75 QIAVANTYACKGLDRIEEKLPILNQPTNQVVANAKGAMTGAKDAVTTTVTGAKDSVASTI 134
Query: 338 TKTLDTVSKTSDFAXXXXXXXXXXXMKIVLPATYLTNVSTNY--VLDKTDELLDKYLP-- 505
T +D + + VL + + +S+ L K++ L+D+YLP
Sbjct: 135 TGVVDRTKGAVTGSVEKTKSVVSGSINTVLRSRVMQLMSSGVENALTKSELLVDQYLPLT 194
Query: 506 -DNNQCNEEPYSNADIVTK------VKRLSGKTQQRLYKRASEQINALLKASLEKLHQIH 664
D + + D+V K + LS K + R Y++A ++ + E + Q+H
Sbjct: 195 KDELEKEAKKVEGFDMVQKPSYYVRLGSLSTKLRSRAYQQALCRVEEAKRKGQETISQLH 254
Query: 665 VLIEEINKRREDLHNS 712
R+++HN+
Sbjct: 255 SAFNLSELARKNVHNA 270
>sp|O67358|TIG_AQUAE Trigger factor (TF)
Length = 478
Score = 35.0 bits (79), Expect = 0.27
Identities = 33/130 (25%), Positives = 61/130 (46%), Gaps = 5/130 (3%)
Frame = +2
Query: 464 VLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASL 643
V DK E+ D +P E + + +++ L T+ K+ E++ + +A++
Sbjct: 321 VADKLVEIHDIPVPQTLLRRELSFLVDRRLRELQALGIDTRYVDIKKIVEEVQPIAEANI 380
Query: 644 EKLHQIHVLIEE--INKRREDL---HNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALR 808
+ + +E I ED+ + +A++Y T+D IKK F N Q+V E R
Sbjct: 381 KLRFILDKYAQEKGIEPTGEDIEAQYKELAEQYGTTVDEIKKY--FKENNLEQVVYEDAR 438
Query: 809 YSKSLKNVVS 838
K+LK ++S
Sbjct: 439 RKKALKEIIS 448
>sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog)
Length = 8545
Score = 33.9 bits (76), Expect = 0.60
Identities = 23/90 (25%), Positives = 46/90 (51%), Gaps = 1/90 (1%)
Frame = +2
Query: 587 QRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPF 766
+R Y+ +++NA + A +E L + +L E+++ +ED++N S +D + +
Sbjct: 8289 ERQYEDTMDKLNAEITAEVELLRTLDILSNELSQCKEDINNP-----SVDVDELSRATML 8343
Query: 767 N-LINHSQIVSEALRYSKSLKNVVSSSTSV 853
N I H + + S+ + V SSTS+
Sbjct: 8344 NDAIAHLENQKVVVARSEKDRKFVESSTSI 8373
>sp|Q6FS63|MAD1_CANGA Spindle assembly checkpoint component MAD1 (Mitotic arrest
deficient protein 1)
Length = 657
Score = 33.5 bits (75), Expect = 0.78
Identities = 26/107 (24%), Positives = 45/107 (42%)
Frame = +2
Query: 452 STNYVLDKTDELLDKYLPDNNQCNEEPYSNADIVTKVKRLSGKTQQRLYKRASEQINALL 631
STN + D LLD Y + E D + + R G ++ R + IN
Sbjct: 382 STNRNDAEYDSLLDNYKQETENLTNELKRLNDSLIEQNRNQGTEMKKRKLRNGDHININY 441
Query: 632 KASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFNL 772
L +L Q ++ +E+ N++ V ++ LD ++K P N+
Sbjct: 442 SQRLNELQQNNLSLEKDNEKL----RLVVEKLEGKLDDLRKTKPKNI 484
>sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546
Length = 62
Score = 32.3 bits (72), Expect = 1.7
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +2
Query: 551 VTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINK 688
+ VK L G++ ++L +E NA+ K + IHV+IEE+ K
Sbjct: 3 IINVKLLEGRSDEQLKNLVTEVTNAVEKTTGANREAIHVIIEEMQK 48
>sp|Q96L93|SNX23_HUMAN Kinesin-like motor protein C20orf23 (Sorting nexin 23)
Length = 1317
Score = 31.6 bits (70), Expect = 3.0
Identities = 21/112 (18%), Positives = 47/112 (41%), Gaps = 8/112 (7%)
Frame = +2
Query: 533 YSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLI--------EEINK 688
Y + K+ + G + + ++ L S EK H + ++ EE+ +
Sbjct: 1069 YEIQQLKQKIYEVDGVQKDHHGTLEGKVASSSLPVSAEKSHLVPLMDARINAYIEEEVQR 1128
Query: 689 RREDLHNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSS 844
R +DLH +++ S + D +K N H+ + L+Y + + + ++
Sbjct: 1129 RLQDLHRVISEGCSTSADTMKDNEKL----HNGTIQRKLKYERMVSRSLGAN 1176
>sp|Q02331|YOL4_CAEEL Hypothetical UPF0028 protein ZK370.4 in chromosome III
Length = 1353
Score = 31.6 bits (70), Expect = 3.0
Identities = 20/61 (32%), Positives = 30/61 (49%), Gaps = 4/61 (6%)
Frame = +2
Query: 617 INALLKASLEKLHQIHVLIEEINKRRED----LHNSVAQEYSNTLDVIKKNNPFNLINHS 784
I LL +L LHQ L E+ +RR D + S + +L + KK PFN+ ++
Sbjct: 370 ITRLLHVTLTTLHQYMGLSSELMRRRRDDDRHRNGSSTSKLQLSLSLKKKEKPFNINDNE 429
Query: 785 Q 787
Q
Sbjct: 430 Q 430
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,516,340
Number of Sequences: 369166
Number of extensions: 1458317
Number of successful extensions: 5025
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4820
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5022
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)