Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_018_F05
(700 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage... 37 0.039
sp|P39723|YAE7_YEAST Hypothetical 72.1 kDa protein in ACS1-... 34 0.33
sp|Q93Y94|RPO1_NICSY DNA-directed RNA polymerase 1, mitocho... 34 0.43
sp|Q27713|DRTS_PLABA Bifunctional dihydrofolate reductase-t... 34 0.43
sp|O67358|TIG_AQUAE Trigger factor (TF) 33 0.56
sp|P77338|KEFA_ECOLI Potassium efflux system kefA (Protein ... 33 0.95
sp|P33110|CRD1_ANSAN Delta crystallin (Argininosuccinate ly... 32 1.2
sp|O15736|TIPD_DICDI Protein tipD 32 1.2
sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546 32 1.2
sp|P07978|HSP2_MOUSE Sperm histone P2 precursor (Protamine ... 32 1.6
>sp|Q9N4M4|ANC1_CAEEL Nuclear anchorage protein 1 (Anchorage 1 protein) (Nesprin homolog)
Length = 8545
Score = 37.4 bits (85), Expect = 0.039
Identities = 25/99 (25%), Positives = 51/99 (51%), Gaps = 1/99 (1%)
Frame = +1
Query: 58 QRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPF 237
+R Y+ +++NA + A +E L + +L E+++ +ED++N S +D + +
Sbjct: 8289 ERQYEDTMDKLNAEITAEVELLRTLDILSNELSQCKEDINNP-----SVDVDELSRATML 8343
Query: 238 N-LINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNNA 351
N I H + + S+ + V SSTS+ L+ ++ A
Sbjct: 8344 NDAIAHLENQKVVVARSEKDRKFVESSTSIDLDQLLAEA 8382
>sp|P39723|YAE7_YEAST Hypothetical 72.1 kDa protein in ACS1-GCV3 intergenic region
Length = 622
Score = 34.3 bits (77), Expect = 0.33
Identities = 34/142 (23%), Positives = 56/142 (39%), Gaps = 12/142 (8%)
Frame = +1
Query: 25 TKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSN 204
TK+ +L +QIN L ++K + HV+ +++K DL N + N
Sbjct: 401 TKINKLKENNWDSYINDLEKQINDL---QIDKSEEFHVIQNQLDKL--DLENYQLKNQLN 455
Query: 205 TLD------------VIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNN 348
TLD IK N NL+ H + L+ ++ + V N
Sbjct: 456 TLDNQKLILSQYESNFIKFNQ--NLLLHLDSIFNILQKILQESSIAQFDRKMKSIKSVPN 513
Query: 349 ALNGLDIVNDNLFKLQNFLQQA 414
AL L+++ L L F++ A
Sbjct: 514 ALKNLNLIQPKLESLYTFIETA 535
>sp|Q93Y94|RPO1_NICSY DNA-directed RNA polymerase 1, mitochondrial precursor (T7
bacteriophage-type single subunit RNA polymerase 1)
(NsRpoT-A)
Length = 1002
Score = 33.9 bits (76), Expect = 0.43
Identities = 31/126 (24%), Positives = 53/126 (42%), Gaps = 7/126 (5%)
Frame = +1
Query: 61 RLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFN 240
R Y A+E A+ S E + +I LIEE+NK E L ++ + T+ + +N
Sbjct: 112 RSYGSAAE---AIASTSEEDIDEIQELIEEMNKENEALKTNLQPKQPKTIGGMGVGK-YN 167
Query: 241 LINHSQIVSE-------ALRYSKSLKNVVSSSTSVTLEYMVNNALNGLDIVNDNLFKLQN 399
L+ QI E A Y + L ++ + L YM + L + + D + Q
Sbjct: 168 LLRRRQIKVETEAWEEAAKEYQELLMDMCEQKLAPNLPYMKSLFLGWFEPLRDAIAAEQK 227
Query: 400 FLQQAR 417
+ +
Sbjct: 228 LCDEGK 233
>sp|Q27713|DRTS_PLABA Bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS)
[Includes: Dihydrofolate reductase ; Thymidylate
synthase ]
Length = 587
Score = 33.9 bits (76), Expect = 0.43
Identities = 40/179 (22%), Positives = 78/179 (43%), Gaps = 27/179 (15%)
Frame = +1
Query: 4 YSNADIVTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVL----IEEINKRRED 171
Y N + ++K K ++ + + ++N + +S L I V+ E I K+ +
Sbjct: 61 YINENNYIRLKWKRDKYMEKHNLKNNVELNTNIISSTNNLQNIVVMGKKSWESIPKKFKP 120
Query: 172 LHNSVAQEYSNTL---DVIKKNNPFN----LINHSQIVSEALRYSKSLKNVVSSSTSVTL 330
L N + S TL D++ +NN N +I + L+ +K K + +SV
Sbjct: 121 LQNRINIILSRTLKKEDIVNENNNENNNVIIIKSVDDLFPILKCTKYYKCFIIGGSSVYK 180
Query: 331 EYM------------VNNALNGLDI----VNDNLFKLQNFLQQARPESVTGELLIHTST 459
E++ +NN+ N D+ +N+NLFK+ + + T + +I++ T
Sbjct: 181 EFLDRNLIKKIYFTRINNSYN-CDVLFPEINENLFKITSISDVYYSNNTTLDFIIYSKT 238
>sp|O67358|TIG_AQUAE Trigger factor (TF)
Length = 478
Score = 33.5 bits (75), Expect = 0.56
Identities = 25/75 (33%), Positives = 38/75 (50%)
Frame = +1
Query: 85 QINALLKASLEKLHQIHVLIEEINKRREDLHNSVAQEYSNTLDVIKKNNPFNLINHSQIV 264
+ N L+ L+K Q IE + E + +A++Y T+D IKK F N Q+V
Sbjct: 377 EANIKLRFILDKYAQ-EKGIEPTGEDIEAQYKELAEQYGTTVDEIKKY--FKENNLEQVV 433
Query: 265 SEALRYSKSLKNVVS 309
E R K+LK ++S
Sbjct: 434 YEDARRKKALKEIIS 448
>sp|P77338|KEFA_ECOLI Potassium efflux system kefA (Protein aefA)
Length = 1120
Score = 32.7 bits (73), Expect = 0.95
Identities = 38/168 (22%), Positives = 72/168 (42%)
Frame = +1
Query: 10 NADIVTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINKRREDLHNSVA 189
NA+I + K L G T L +Q + + S HQ+ +L E +N +R L A
Sbjct: 212 NAEIDQQRKSLEGNTV--LQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTA 269
Query: 190 QEYSNTLDVIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSSTSVTLEYMVNNALNGLDI 369
QE + + + I + +V + L ++ L + ++T NG +
Sbjct: 270 QEAVSPDEAAR-------IQANPLVKQELEINQQLSQRLITATE-----------NGNQL 311
Query: 370 VNDNLFKLQNFLQQARPESVTGELLIHTSTAVTEIRIALDSFIYHMKR 513
+ N+ K++N+L++A + E I AV + + L +Y ++
Sbjct: 312 MQQNI-KVKNWLERA----LQSERNIKEQIAVLKGSLLLSRILYQQQQ 354
>sp|P33110|CRD1_ANSAN Delta crystallin (Argininosuccinate lyase)
Length = 466
Score = 32.3 bits (72), Expect = 1.2
Identities = 36/165 (21%), Positives = 69/165 (41%), Gaps = 18/165 (10%)
Frame = +1
Query: 10 NADIVTKVKRLSGKTQQRL----------YKRASEQINALLKASLEK-LHQIHVLIEE-- 150
+ D + ++ S T+QRL Y +A E+ L K LEK L + + EE
Sbjct: 16 STDPIMQMLSTSMSTEQRLSEVDIQASIAYAKALEKAGILTKTELEKILSGLEKISEEWS 75
Query: 151 -----INKRREDLHNSVAQEYSNTLDVIKKNNPFNLINHSQIVSEALRYSKSLKNVVSSS 315
+ + ED+H + + + I + Q+V++ + K+ +V+S+
Sbjct: 76 KGVFVVTQSDEDIHTANERRLKELIGDIAGKLNTGRSRNEQVVTDLKLFMKNSLSVISTH 135
Query: 316 TSVTLEYMVNNALNGLDIVNDNLFKLQNFLQQARPESVTGELLIH 450
++ +V A +D++ LQ+A+P + LL H
Sbjct: 136 LLQLIKTLVERAAIEIDVILPG----YTHLQKAQPIRWSQFLLSH 176
>sp|O15736|TIPD_DICDI Protein tipD
Length = 612
Score = 32.3 bits (72), Expect = 1.2
Identities = 40/176 (22%), Positives = 76/176 (43%), Gaps = 5/176 (2%)
Frame = +1
Query: 49 KTQQRLYKRASEQINALLKASLEKLHQIHVLIEEIN----KRREDLHNSVAQEYSNTLDV 216
KTQQ+ +S+ ++ +S ++ + IEE+ K +EDL NS + N +
Sbjct: 99 KTQQQPPSGSSKMDSSSSSSSSNRVSGMGSTIEEMEQKLFKLQEDLTNSYKRNADNASSI 158
Query: 217 IKKNNPFNLINHSQIVSEALRYSKSLKNVVSSSTSV-TLEYMVNNALNGLDIVNDNLFKL 393
+ N+ N ++++S+ + + + S+ LE +V N I+ D L L
Sbjct: 159 LLLNDK-NKDLQNELMSKEIEIERIRSTIQQDLDSIKRLEMVVIEKENVSQIIRDELSSL 217
Query: 394 QNFLQQARPESVTGELLIHTSTAVTEIRIALDSFIYHMKRYSRKKFHGEEITEMHE 561
Q +H + V ++ S + +R+ RKK EE ++M+E
Sbjct: 218 QT-------------EFLHNESKVVKLEQENSSLV---ERWLRKK--NEEASKMNE 255
>sp|Q4L670|Y1546_STAHJ Probable tautomerase SH1546
Length = 62
Score = 32.3 bits (72), Expect = 1.2
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +1
Query: 22 VTKVKRLSGKTQQRLYKRASEQINALLKASLEKLHQIHVLIEEINK 159
+ VK L G++ ++L +E NA+ K + IHV+IEE+ K
Sbjct: 3 IINVKLLEGRSDEQLKNLVTEVTNAVEKTTGANREAIHVIIEEMQK 48
>sp|P07978|HSP2_MOUSE Sperm histone P2 precursor (Protamine MP2)
Length = 107
Score = 32.0 bits (71), Expect = 1.6
Identities = 25/61 (40%), Positives = 29/61 (47%)
Frame = +3
Query: 399 LSPASET*KRYWRTLDSHEHCRHRNTDRTRLLHLPHEAIFEKEIPRRRNYGNARNECEHR 578
LSP E + Y RT H H RHR R R LH H+ + RRR R+ C HR
Sbjct: 32 LSP--ERVEDYGRTHRGHHHHRHRRCSRKR-LHRIHKR--RRSCRRRR-----RHSCRHR 81
Query: 579 R 581
R
Sbjct: 82 R 82
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,960,587
Number of Sequences: 369166
Number of extensions: 1480702
Number of successful extensions: 5039
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4834
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5030
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6073541875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)