Planarian EST Database

DrC_01064

Cluster detail
GO Category : Not Available
Consensus Sequence :
>DrC_01064 AATTCGGCACGAGGCTTCGTGCACAATTAATTTTAATAGGAGCATTTAATTAACTGAAAA TGAGTGATTTAAACAGTCCAGTTTTTATGTCTCCATTTTTAAATTTCGATCCGTCATTAT TAGTATCAAATCCTAAAGATCAGTACATACATGTTGATGGTTCAGAAGAAAGGGGTAGAT TCGAAAGATCATGTTGTGAAATTGGGGGAATGGTAATATTAGGTGGTGCATATGGTGGAT TTAAATCTATTATCCCAGCTTTATGGTCACGTTCATTAAGAGAAGCAAACCGTAGTTACT TAATAAGCCAAATTGCAAAAAGTTCTGCAACATTTGCTCAATCGCTTGGCGGAATTGGTT TGCTGTATGCGGGTTGCGATTCTATTCTTCATTATAGTCTGAAGCAAGAAGATGAAGTAA CTAACATTCTCGCAGCAACAACCACTGGTTTAATTCATGGATTTGGAAGTCTTTCTTGGC AGAGAGCTATTAAAGGTGGAGGAGTTGGTCTAGCTGTCGGATGCGCGATTGGGGCATTTT CAAACTATGATAAATTAAAGTCTTCTTTTAGAGGAATGTTGTATTAATTTTAATAAACTT AGCGATGTTATAGATAGTCATGCTCTGAGCATTTATCGATATGTACATTTCAATATATGA AAAATGATGAAATTTTATTAATTTTTTTCTTAATTCAACATTTGTGTTAGATAAAATGAT AATCAATATTGTC

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01064
         (733 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q7T2P6|TIM23_BRARE  Mitochondrial import inner membrane t...    75   1e-13
sp|O14925|TIM23_HUMAN  Mitochondrial import inner membrane t...    73   7e-13
sp|O35093|TIM23_RAT  Mitochondrial import inner membrane tra...    72   1e-12
sp|Q9WTQ8|TIM23_MOUSE  Mitochondrial import inner membrane t...    72   2e-12
sp|Q12328|TIM22_YEAST  Mitochondrial import inner membrane t...    33   0.60 
sp|P87146|TIM22_SCHPO  Mitochondrial import inner membrane t...    33   1.0  
sp|O67178|YA88_AQUAE  Hypothetical protein AQ_1088                 31   3.0  
sp|P32897|TIM23_YEAST  Mitochondrial import inner membrane t...    30   8.6

>sp|Q7T2P6|TIM23_BRARE Mitochondrial import inner membrane translocase subunit Tim23
          Length = 208

 Score = 75.5 bits (184), Expect = 1e-13
 Identities = 46/166 (27%), Positives = 83/166 (50%), Gaps = 8/166 (4%)
 Frame = +3

Query: 87  MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYGGFKSIIPAL- 263
           +SP+LN DP  L+ +  +  +    ++ RGRFE +   IGG  I G A+G    +   L 
Sbjct: 42  LSPYLNVDPRYLIQDTDEFILPTGANKTRGRFELAFFTIGGCCITGAAFGTLNGLRMGLS 101

Query: 264 ------WSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 425
                 WS+         +++ + +  A++A +LG + LLY+     +  +   ED++  
Sbjct: 102 ETRDMPWSK----PRNVQILNMVTRQGASWANTLGSVALLYSVFGVAIEKARGAEDDLNT 157

Query: 426 ILAATTTGLIHGFGSLSWQRAI-KGGGVGLAVGCAIGAFSNYDKLK 560
           + A T TG++  F S    + + +GG +GLA+      ++N+D LK
Sbjct: 158 VAAGTLTGMV--FKSTGGLKGVARGGLIGLAMSGLYALYNNWDHLK 201

>sp|O14925|TIM23_HUMAN Mitochondrial import inner membrane translocase subunit Tim23
          Length = 209

 Score = 73.2 bits (178), Expect = 7e-13
 Identities = 46/168 (27%), Positives = 82/168 (48%), Gaps = 8/168 (4%)
 Frame = +3

Query: 87  MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYG-------GFK 245
           +SP+LN DP  LV +  +  +    ++ RGRFE +   IGG  + G A+G       G K
Sbjct: 42  LSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLK 101

Query: 246 SIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 425
                 WS+         +++ + +  A +A +LG + LLY+    I+  +   ED++  
Sbjct: 102 ETQNMAWSK----PRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNT 157

Query: 426 ILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 566
           + A T TG+++   G L  +   +GG  GL +      ++N++ +K S
Sbjct: 158 VAAGTMTGMLYKCTGGL--RGIARGGLTGLTLTSLYALYNNWEHMKGS 203

>sp|O35093|TIM23_RAT Mitochondrial import inner membrane translocase subunit Tim23
          Length = 209

 Score = 72.4 bits (176), Expect = 1e-12
 Identities = 46/168 (27%), Positives = 81/168 (48%), Gaps = 8/168 (4%)
 Frame = +3

Query: 87  MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYG-------GFK 245
           +SP+LN DP  LV +  +  +    ++ RGRFE +   IGG  + G A+G       G K
Sbjct: 42  LSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGALNGLRLGLK 101

Query: 246 SIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 425
                 WS+         +++ + +  A +A +LG + LLY+    I+  +   ED+   
Sbjct: 102 ETQSMPWSK----PRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDFNT 157

Query: 426 ILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 566
           + A T TG+++   G L  +   +GG  GL +      ++N++ +K S
Sbjct: 158 VAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSVYALYNNWEHMKGS 203

>sp|Q9WTQ8|TIM23_MOUSE Mitochondrial import inner membrane translocase subunit Tim23
          Length = 209

 Score = 71.6 bits (174), Expect = 2e-12
 Identities = 46/168 (27%), Positives = 81/168 (48%), Gaps = 8/168 (4%)
 Frame = +3

Query: 87  MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYG-------GFK 245
           +SP+LN DP  LV +  +  +    ++ RGRFE +   IGG  + G A+G       G K
Sbjct: 42  LSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLK 101

Query: 246 SIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 425
                 WS+         +++ + +  A +A +LG + LLY+    I+  +   ED++  
Sbjct: 102 ETQSMAWSK----PRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNT 157

Query: 426 ILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 566
           + A T TG+++   G L  +   +GG  GL +      ++N+  +K S
Sbjct: 158 VAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSLYALYNNWVHMKGS 203

>sp|Q12328|TIM22_YEAST Mitochondrial import inner membrane translocase subunit TIM22
          Length = 207

 Score = 33.5 bits (75), Expect = 0.60
 Identities = 24/81 (29%), Positives = 43/81 (53%), Gaps = 3/81 (3%)
 Frame = +3

Query: 306 SQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTNILAA---TTTGLIHGFGSLS 476
           + + K S + A++ G IG++YAG + ++  SL+ ++++ N + A   T  GL +  G   
Sbjct: 116 TDMGKKSYSSAKNFGYIGMIYAGVECVIE-SLRAKNDIYNGVTAGFFTGAGLAYKAGP-- 172

Query: 477 WQRAIKGGGVGLAVGCAIGAF 539
            Q A+ GG    A   AI  +
Sbjct: 173 -QAALMGGAGFAAFSAAIDLY 192

>sp|P87146|TIM22_SCHPO Mitochondrial import inner membrane translocase subunit tim22
          Length = 175

 Score = 32.7 bits (73), Expect = 1.0
 Identities = 30/106 (28%), Positives = 47/106 (44%), Gaps = 6/106 (5%)
 Frame = +3

Query: 219 LGGAYGGFKS------IIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCD 380
           LGG +G F S      I P ++ +  RE  R       ++S +T A++ G +GL+Y+G +
Sbjct: 60  LGGIFGMFISSLDLQHIDPKIYEKPFREQIRIQARDMGSRSFST-AKNFGLLGLIYSGSE 118

Query: 381 SILHYSLKQEDEVTNILAATTTGLIHGFGSLSWQRAIKGGGVGLAV 518
             +     + D    I A   TG        S  +AI  GG G  +
Sbjct: 119 CCIEAFRAKTDIYNAIAAGVFTG--GALAVRSGPKAIVLGGAGFGL 162

>sp|O67178|YA88_AQUAE Hypothetical protein AQ_1088
          Length = 761

 Score = 31.2 bits (69), Expect = 3.0
 Identities = 24/75 (32%), Positives = 37/75 (49%), Gaps = 11/75 (14%)
 Frame = +3

Query: 300 LISQIAKSSATFAQSLGGIGLLY------AGCDSILHYSLKQEDEVTNILAATTTGLIHG 461
           L+SQ++++S   AQ L  +GLLY         + +L  +LK  D V + L A    L + 
Sbjct: 57  LLSQVSENSLNSAQGLSDLGLLYFFLGRVEDAERVLKKALKFSD-VDDALYARLGALYYS 115

Query: 462 FGSLS-----WQRAI 491
            G L      W+RA+
Sbjct: 116 QGKLEEAQHYWERAL 130

>sp|P32897|TIM23_YEAST Mitochondrial import inner membrane translocase subunit TIM23
           (Mitochondrial protein import protein 3) (Mitochondrial
           protein import protein MAS6) (Membrane import machinery
           protein MIM23)
          Length = 222

 Score = 29.6 bits (65), Expect = 8.6
 Identities = 30/139 (21%), Positives = 57/139 (41%), Gaps = 7/139 (5%)
 Frame = +3

Query: 135 KDQYIHVDGSE----ERGRFERSCCEIGGMVILGGAYGGFKSIIPALWS---RSLREANR 293
           ++Q   ++GS+     RG  +  C   G + +LG   GGF  ++  L +    S  +   
Sbjct: 75  EEQLSSLEGSQGLIPSRGWTDDLCYGTGAVYLLGLGIGGFSGMMQGLQNIPPNSPGKLQL 134

Query: 294 SYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTNILAATTTGLIHGFGSL 473
           + +++ I K       + G + L Y   +S +     + D   +I A   TG +  F S 
Sbjct: 135 NTVLNHITKRGPFLGNNAGILALSYNIINSTIDALRGKHDTAGSIGAGALTGAL--FKSS 192

Query: 474 SWQRAIKGGGVGLAVGCAI 530
              + +      +A  CA+
Sbjct: 193 KGLKPMGYSSAMVAAACAV 211

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,511,133
Number of Sequences: 369166
Number of extensions: 1297851
Number of successful extensions: 3612
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3402
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3596
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6534486000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01064

Planaria EST Database

DrC_01064

BLASTX 2.2.13 [Nov-27-2005]

Cluster detail