Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_E12
(327 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q7T2P6|TIM23_BRARE Mitochondrial import inner membrane t... 34 0.090
sp|O14925|TIM23_HUMAN Mitochondrial import inner membrane t... 32 0.45
sp|O35093|TIM23_RAT Mitochondrial import inner membrane tra... 32 0.58
sp|Q9WTQ8|TIM23_MOUSE Mitochondrial import inner membrane t... 30 1.7
sp|Q5KZL2|NORM_GEOKA Probable multidrug resistance protein ... 28 4.9
sp|Q7M4F4|CUD1_SCHGR Endocuticle structural glycoprotein Sg... 28 4.9
sp|P43077|HEX1_CANAL Beta-hexosaminidase precursor (N-acety... 28 8.4
>sp|Q7T2P6|TIM23_BRARE Mitochondrial import inner membrane translocase subunit Tim23
Length = 208
Score = 34.3 bits (77), Expect = 0.090
Identities = 20/70 (28%), Positives = 37/70 (52%), Gaps = 1/70 (1%)
Frame = +3
Query: 12 IGLLYAGCDSILHYSLKQEDEVTNILAATTTGLIHGFGSLSWQRAI-KGGGVGLAVGCAI 188
+ LLY+ + + ED++ + A T TG++ F S + + +GG +GLA+
Sbjct: 134 VALLYSVFGVAIEKARGAEDDLNTVAAGTLTGMV--FKSTGGLKGVARGGLIGLAMSGLY 191
Query: 189 GAFSNYDKLK 218
++N+D LK
Sbjct: 192 ALYNNWDHLK 201
>sp|O14925|TIM23_HUMAN Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 32.0 bits (71), Expect = 0.45
Identities = 19/72 (26%), Positives = 37/72 (51%), Gaps = 1/72 (1%)
Frame = +3
Query: 12 IGLLYAGCDSILHYSLKQEDEVTNILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAI 188
+ LLY+ I+ + ED++ + A T TG+++ G L + +GG GL +
Sbjct: 134 LALLYSAFGVIIEKTRGAEDDLNTVAAGTMTGMLYKCTGGL--RGIARGGLTGLTLTSLY 191
Query: 189 GAFSNYDKLKSS 224
++N++ +K S
Sbjct: 192 ALYNNWEHMKGS 203
>sp|O35093|TIM23_RAT Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 31.6 bits (70), Expect = 0.58
Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 1/72 (1%)
Frame = +3
Query: 12 IGLLYAGCDSILHYSLKQEDEVTNILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAI 188
+ LLY+ I+ + ED+ + A T TG+++ G L + +GG GL +
Sbjct: 134 LALLYSAFGVIIEKTRGAEDDFNTVAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSVY 191
Query: 189 GAFSNYDKLKSS 224
++N++ +K S
Sbjct: 192 ALYNNWEHMKGS 203
>sp|Q9WTQ8|TIM23_MOUSE Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 30.0 bits (66), Expect = 1.7
Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 1/72 (1%)
Frame = +3
Query: 12 IGLLYAGCDSILHYSLKQEDEVTNILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAI 188
+ LLY+ I+ + ED++ + A T TG+++ G L + +GG GL +
Sbjct: 134 LALLYSAFGVIIEKTRGAEDDLNTVAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSLY 191
Query: 189 GAFSNYDKLKSS 224
++N+ +K S
Sbjct: 192 ALYNNWVHMKGS 203
>sp|Q5KZL2|NORM_GEOKA Probable multidrug resistance protein norM (Multidrug-efflux
transporter)
Length = 455
Score = 28.5 bits (62), Expect = 4.9
Identities = 13/42 (30%), Positives = 24/42 (57%), Gaps = 3/42 (7%)
Frame = +3
Query: 72 EVTNILAATTTGLIHGFGSLS---WQRAIKGGGVGLAVGCAI 188
+V++ +AA G + G+ ++ W + GVGL +GCA+
Sbjct: 368 QVSDAIAAPIQGALRGYKEVNAVFWSALLAYWGVGLPLGCAL 409
>sp|Q7M4F4|CUD1_SCHGR Endocuticle structural glycoprotein SgAbd-1
Length = 184
Score = 28.5 bits (62), Expect = 4.9
Identities = 15/43 (34%), Positives = 22/43 (51%)
Frame = -3
Query: 193 APIAHPTARPTPPPLIALCQERLPNP*IKPVVVAARMLVTSSS 65
+P A P PPP + + LP P +P +V AR + +SS
Sbjct: 29 SPPASRAPPPPPPPRPVVPSQPLPAPAPQPAIVPARTIPQASS 71
>sp|P43077|HEX1_CANAL Beta-hexosaminidase precursor (N-acetyl-beta-glucosaminidase)
(Beta-GlcNAcase) (Beta-N-acetylhexosaminidase)
Length = 562
Score = 27.7 bits (60), Expect = 8.4
Identities = 14/27 (51%), Positives = 17/27 (62%)
Frame = +3
Query: 51 YSLKQEDEVTNILAATTTGLIHGFGSL 131
Y+LK + NI AATT G +HG SL
Sbjct: 114 YTLKINTDGINIHAATTWGALHGLVSL 140
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,980,220
Number of Sequences: 369166
Number of extensions: 536728
Number of successful extensions: 1863
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1708
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1853
length of database: 68,354,980
effective HSP length: 77
effective length of database: 54,130,385
effective search space used: 1678041935
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)