Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_E22
(610 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q7T2P6|TIM23_BRARE Mitochondrial import inner membrane t... 75 1e-13
sp|O14925|TIM23_HUMAN Mitochondrial import inner membrane t... 73 5e-13
sp|O35093|TIM23_RAT Mitochondrial import inner membrane tra... 72 8e-13
sp|Q9WTQ8|TIM23_MOUSE Mitochondrial import inner membrane t... 72 1e-12
sp|Q12328|TIM22_YEAST Mitochondrial import inner membrane t... 33 0.43
sp|P87146|TIM22_SCHPO Mitochondrial import inner membrane t... 33 0.74
sp|O67178|YA88_AQUAE Hypothetical protein AQ_1088 31 2.2
sp|P32897|TIM23_YEAST Mitochondrial import inner membrane t... 30 6.3
sp|P06865|HEXA_HUMAN Beta-hexosaminidase alpha chain precur... 29 8.2
sp|Q6N5Q9|LPXD_RHOPA UDP-3-O-[3-hydroxymyristoyl] glucosami... 29 8.2
>sp|Q7T2P6|TIM23_BRARE Mitochondrial import inner membrane translocase subunit Tim23
Length = 208
Score = 75.5 bits (184), Expect = 1e-13
Identities = 46/166 (27%), Positives = 83/166 (50%), Gaps = 8/166 (4%)
Frame = +3
Query: 81 MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYGGFKSIIPAL- 257
+SP+LN DP L+ + + + ++ RGRFE + IGG I G A+G + L
Sbjct: 42 LSPYLNVDPRYLIQDTDEFILPTGANKTRGRFELAFFTIGGCCITGAAFGTLNGLRMGLS 101
Query: 258 ------WSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 419
WS+ +++ + + A++A +LG + LLY+ + + ED++
Sbjct: 102 ETRDMPWSK----PRNVQILNMVTRQGASWANTLGSVALLYSVFGVAIEKARGAEDDLNT 157
Query: 420 ILAATTTGLIHGFGSLSWQRAI-KGGGVGLAVGCAIGAFSNYDKLK 554
+ A T TG++ F S + + +GG +GLA+ ++N+D LK
Sbjct: 158 VAAGTLTGMV--FKSTGGLKGVARGGLIGLAMSGLYALYNNWDHLK 201
>sp|O14925|TIM23_HUMAN Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 73.2 bits (178), Expect = 5e-13
Identities = 46/168 (27%), Positives = 82/168 (48%), Gaps = 8/168 (4%)
Frame = +3
Query: 81 MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYG-------GFK 239
+SP+LN DP LV + + + ++ RGRFE + IGG + G A+G G K
Sbjct: 42 LSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLK 101
Query: 240 SIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 419
WS+ +++ + + A +A +LG + LLY+ I+ + ED++
Sbjct: 102 ETQNMAWSK----PRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNT 157
Query: 420 ILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 560
+ A T TG+++ G L + +GG GL + ++N++ +K S
Sbjct: 158 VAAGTMTGMLYKCTGGL--RGIARGGLTGLTLTSLYALYNNWEHMKGS 203
>sp|O35093|TIM23_RAT Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 72.4 bits (176), Expect = 8e-13
Identities = 46/168 (27%), Positives = 81/168 (48%), Gaps = 8/168 (4%)
Frame = +3
Query: 81 MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYG-------GFK 239
+SP+LN DP LV + + + ++ RGRFE + IGG + G A+G G K
Sbjct: 42 LSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGALNGLRLGLK 101
Query: 240 SIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 419
WS+ +++ + + A +A +LG + LLY+ I+ + ED+
Sbjct: 102 ETQSMPWSK----PRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDFNT 157
Query: 420 ILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 560
+ A T TG+++ G L + +GG GL + ++N++ +K S
Sbjct: 158 VAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSVYALYNNWEHMKGS 203
>sp|Q9WTQ8|TIM23_MOUSE Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 71.6 bits (174), Expect = 1e-12
Identities = 46/168 (27%), Positives = 81/168 (48%), Gaps = 8/168 (4%)
Frame = +3
Query: 81 MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYG-------GFK 239
+SP+LN DP LV + + + ++ RGRFE + IGG + G A+G G K
Sbjct: 42 LSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLK 101
Query: 240 SIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 419
WS+ +++ + + A +A +LG + LLY+ I+ + ED++
Sbjct: 102 ETQSMAWSK----PRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNT 157
Query: 420 ILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 560
+ A T TG+++ G L + +GG GL + ++N+ +K S
Sbjct: 158 VAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSLYALYNNWVHMKGS 203
>sp|Q12328|TIM22_YEAST Mitochondrial import inner membrane translocase subunit TIM22
Length = 207
Score = 33.5 bits (75), Expect = 0.43
Identities = 24/81 (29%), Positives = 43/81 (53%), Gaps = 3/81 (3%)
Frame = +3
Query: 300 SQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTNILAA---TTTGLIHGFGSLS 470
+ + K S + A++ G IG++YAG + ++ SL+ ++++ N + A T GL + G
Sbjct: 116 TDMGKKSYSSAKNFGYIGMIYAGVECVIE-SLRAKNDIYNGVTAGFFTGAGLAYKAGP-- 172
Query: 471 WQRAIKGGGVGLAVGCAIGAF 533
Q A+ GG A AI +
Sbjct: 173 -QAALMGGAGFAAFSAAIDLY 192
>sp|P87146|TIM22_SCHPO Mitochondrial import inner membrane translocase subunit tim22
Length = 175
Score = 32.7 bits (73), Expect = 0.74
Identities = 30/106 (28%), Positives = 47/106 (44%), Gaps = 6/106 (5%)
Frame = +3
Query: 213 LGGAYGGFKS------IIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCD 374
LGG +G F S I P ++ + RE R ++S +T A++ G +GL+Y+G +
Sbjct: 60 LGGIFGMFISSLDLQHIDPKIYEKPFREQIRIQARDMGSRSFST-AKNFGLLGLIYSGSE 118
Query: 375 SILHYSLKQEDEVTNILAATTTGLIHGFGSLSWQRAIKGGGVGLAV 512
+ + D I A TG S +AI GG G +
Sbjct: 119 CCIEAFRAKTDIYNAIAAGVFTG--GALAVRSGPKAIVLGGAGFGL 162
>sp|O67178|YA88_AQUAE Hypothetical protein AQ_1088
Length = 761
Score = 31.2 bits (69), Expect = 2.2
Identities = 24/75 (32%), Positives = 37/75 (49%), Gaps = 11/75 (14%)
Frame = +3
Query: 294 LISQIAKSSATFAQSLGGIGLLY------AGCDSILHYSLKQEDEVTNILAATTTGLIHG 455
L+SQ++++S AQ L +GLLY + +L +LK D V + L A L +
Sbjct: 57 LLSQVSENSLNSAQGLSDLGLLYFFLGRVEDAERVLKKALKFSD-VDDALYARLGALYYS 115
Query: 456 FGSLS-----WQRAI 485
G L W+RA+
Sbjct: 116 QGKLEEAQHYWERAL 130
>sp|P32897|TIM23_YEAST Mitochondrial import inner membrane translocase subunit TIM23
(Mitochondrial protein import protein 3) (Mitochondrial
protein import protein MAS6) (Membrane import machinery
protein MIM23)
Length = 222
Score = 29.6 bits (65), Expect = 6.3
Identities = 30/139 (21%), Positives = 57/139 (41%), Gaps = 7/139 (5%)
Frame = +3
Query: 129 KDQYIHVDGSE----ERGRFERSCCEIGGMVILGGAYGGFKSIIPALWS---RSLREANR 287
++Q ++GS+ RG + C G + +LG GGF ++ L + S +
Sbjct: 75 EEQLSSLEGSQGLIPSRGWTDDLCYGTGAVYLLGLGIGGFSGMMQGLQNIPPNSPGKLQL 134
Query: 288 SYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTNILAATTTGLIHGFGSL 467
+ +++ I K + G + L Y +S + + D +I A TG + F S
Sbjct: 135 NTVLNHITKRGPFLGNNAGILALSYNIINSTIDALRGKHDTAGSIGAGALTGAL--FKSS 192
Query: 468 SWQRAIKGGGVGLAVGCAI 524
+ + +A CA+
Sbjct: 193 KGLKPMGYSSAMVAAACAV 211
>sp|P06865|HEXA_HUMAN Beta-hexosaminidase alpha chain precursor
(N-acetyl-beta-glucosaminidase)
(Beta-N-acetylhexosaminidase) (Hexosaminidase A)
Length = 529
Score = 29.3 bits (64), Expect = 8.2
Identities = 23/68 (33%), Positives = 31/68 (45%)
Frame = +3
Query: 129 KDQYIHVDGSEERGRFERSCCEIGGMVILGGAYGGFKSIIPALWSRSLREANRSYLISQI 308
KD Y+ V+ G E+ IGG + G Y +++P LW R+ A R L S
Sbjct: 432 KDFYV-VEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAER--LWSNK 488
Query: 309 AKSSATFA 332
S TFA
Sbjct: 489 LTSDLTFA 496
>sp|Q6N5Q9|LPXD_RHOPA UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
Length = 360
Score = 29.3 bits (64), Expect = 8.2
Identities = 21/66 (31%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = +3
Query: 345 GIGLLYAGCDSILHYSLKQEDEVT-NILAATTTGLIHGFGSLSWQRAIKGGGVGLAVGCA 521
G+G +++H + K EDEVT LA + G G++ A+ GV + C
Sbjct: 113 GVGAPGIAPTAVIHETAKLEDEVTVEPLAVIGPDVEIGSGTVIGAGAVIAAGVKIGRDCD 172
Query: 522 IGAFSN 539
IGA S+
Sbjct: 173 IGAGSH 178
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,547,055
Number of Sequences: 369166
Number of extensions: 1096975
Number of successful extensions: 3186
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2994
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3170
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4748907085
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)