Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_I10
(708 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q7T2P6|TIM23_BRARE Mitochondrial import inner membrane t... 75 1e-13
sp|O14925|TIM23_HUMAN Mitochondrial import inner membrane t... 73 6e-13
sp|O35093|TIM23_RAT Mitochondrial import inner membrane tra... 72 1e-12
sp|Q9WTQ8|TIM23_MOUSE Mitochondrial import inner membrane t... 72 2e-12
sp|Q12328|TIM22_YEAST Mitochondrial import inner membrane t... 33 0.57
sp|P87146|TIM22_SCHPO Mitochondrial import inner membrane t... 33 0.97
sp|O67178|YA88_AQUAE Hypothetical protein AQ_1088 31 2.8
sp|P32897|TIM23_YEAST Mitochondrial import inner membrane t... 30 8.2
>sp|Q7T2P6|TIM23_BRARE Mitochondrial import inner membrane translocase subunit Tim23
Length = 208
Score = 75.5 bits (184), Expect = 1e-13
Identities = 46/166 (27%), Positives = 83/166 (50%), Gaps = 8/166 (4%)
Frame = +2
Query: 62 MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYGGFKSIIPAL- 238
+SP+LN DP L+ + + + ++ RGRFE + IGG I G A+G + L
Sbjct: 42 LSPYLNVDPRYLIQDTDEFILPTGANKTRGRFELAFFTIGGCCITGAAFGTLNGLRMGLS 101
Query: 239 ------WSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 400
WS+ +++ + + A++A +LG + LLY+ + + ED++
Sbjct: 102 ETRDMPWSK----PRNVQILNMVTRQGASWANTLGSVALLYSVFGVAIEKARGAEDDLNT 157
Query: 401 ILAATTTGLIHGFGSLSWQRAI-KGGGVGLAVGCAIGAFSNYDKLK 535
+ A T TG++ F S + + +GG +GLA+ ++N+D LK
Sbjct: 158 VAAGTLTGMV--FKSTGGLKGVARGGLIGLAMSGLYALYNNWDHLK 201
>sp|O14925|TIM23_HUMAN Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 73.2 bits (178), Expect = 6e-13
Identities = 46/168 (27%), Positives = 82/168 (48%), Gaps = 8/168 (4%)
Frame = +2
Query: 62 MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYG-------GFK 220
+SP+LN DP LV + + + ++ RGRFE + IGG + G A+G G K
Sbjct: 42 LSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLK 101
Query: 221 SIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 400
WS+ +++ + + A +A +LG + LLY+ I+ + ED++
Sbjct: 102 ETQNMAWSK----PRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNT 157
Query: 401 ILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 541
+ A T TG+++ G L + +GG GL + ++N++ +K S
Sbjct: 158 VAAGTMTGMLYKCTGGL--RGIARGGLTGLTLTSLYALYNNWEHMKGS 203
>sp|O35093|TIM23_RAT Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 72.4 bits (176), Expect = 1e-12
Identities = 46/168 (27%), Positives = 81/168 (48%), Gaps = 8/168 (4%)
Frame = +2
Query: 62 MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYG-------GFK 220
+SP+LN DP LV + + + ++ RGRFE + IGG + G A+G G K
Sbjct: 42 LSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGALNGLRLGLK 101
Query: 221 SIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 400
WS+ +++ + + A +A +LG + LLY+ I+ + ED+
Sbjct: 102 ETQSMPWSK----PRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDFNT 157
Query: 401 ILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 541
+ A T TG+++ G L + +GG GL + ++N++ +K S
Sbjct: 158 VAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSVYALYNNWEHMKGS 203
>sp|Q9WTQ8|TIM23_MOUSE Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 71.6 bits (174), Expect = 2e-12
Identities = 46/168 (27%), Positives = 81/168 (48%), Gaps = 8/168 (4%)
Frame = +2
Query: 62 MSPFLNFDPSLLVSNPKDQYIHVDGSEERGRFERSCCEIGGMVILGGAYG-------GFK 220
+SP+LN DP LV + + + ++ RGRFE + IGG + G A+G G K
Sbjct: 42 LSPYLNVDPRYLVQDTDEFILPTGANKTRGRFELAFFTIGGCCMTGAAFGAMNGLRLGLK 101
Query: 221 SIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTN 400
WS+ +++ + + A +A +LG + LLY+ I+ + ED++
Sbjct: 102 ETQSMAWSK----PRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRGAEDDLNT 157
Query: 401 ILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 541
+ A T TG+++ G L + +GG GL + ++N+ +K S
Sbjct: 158 VAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSLYALYNNWVHMKGS 203
>sp|Q12328|TIM22_YEAST Mitochondrial import inner membrane translocase subunit TIM22
Length = 207
Score = 33.5 bits (75), Expect = 0.57
Identities = 24/81 (29%), Positives = 43/81 (53%), Gaps = 3/81 (3%)
Frame = +2
Query: 281 SQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTNILAA---TTTGLIHGFGSLS 451
+ + K S + A++ G IG++YAG + ++ SL+ ++++ N + A T GL + G
Sbjct: 116 TDMGKKSYSSAKNFGYIGMIYAGVECVIE-SLRAKNDIYNGVTAGFFTGAGLAYKAGP-- 172
Query: 452 WQRAIKGGGVGLAVGCAIGAF 514
Q A+ GG A AI +
Sbjct: 173 -QAALMGGAGFAAFSAAIDLY 192
>sp|P87146|TIM22_SCHPO Mitochondrial import inner membrane translocase subunit tim22
Length = 175
Score = 32.7 bits (73), Expect = 0.97
Identities = 30/106 (28%), Positives = 47/106 (44%), Gaps = 6/106 (5%)
Frame = +2
Query: 194 LGGAYGGFKS------IIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCD 355
LGG +G F S I P ++ + RE R ++S +T A++ G +GL+Y+G +
Sbjct: 60 LGGIFGMFISSLDLQHIDPKIYEKPFREQIRIQARDMGSRSFST-AKNFGLLGLIYSGSE 118
Query: 356 SILHYSLKQEDEVTNILAATTTGLIHGFGSLSWQRAIKGGGVGLAV 493
+ + D I A TG S +AI GG G +
Sbjct: 119 CCIEAFRAKTDIYNAIAAGVFTG--GALAVRSGPKAIVLGGAGFGL 162
>sp|O67178|YA88_AQUAE Hypothetical protein AQ_1088
Length = 761
Score = 31.2 bits (69), Expect = 2.8
Identities = 24/75 (32%), Positives = 37/75 (49%), Gaps = 11/75 (14%)
Frame = +2
Query: 275 LISQIAKSSATFAQSLGGIGLLY------AGCDSILHYSLKQEDEVTNILAATTTGLIHG 436
L+SQ++++S AQ L +GLLY + +L +LK D V + L A L +
Sbjct: 57 LLSQVSENSLNSAQGLSDLGLLYFFLGRVEDAERVLKKALKFSD-VDDALYARLGALYYS 115
Query: 437 FGSLS-----WQRAI 466
G L W+RA+
Sbjct: 116 QGKLEEAQHYWERAL 130
>sp|P32897|TIM23_YEAST Mitochondrial import inner membrane translocase subunit TIM23
(Mitochondrial protein import protein 3) (Mitochondrial
protein import protein MAS6) (Membrane import machinery
protein MIM23)
Length = 222
Score = 29.6 bits (65), Expect = 8.2
Identities = 30/139 (21%), Positives = 57/139 (41%), Gaps = 7/139 (5%)
Frame = +2
Query: 110 KDQYIHVDGSE----ERGRFERSCCEIGGMVILGGAYGGFKSIIPALWS---RSLREANR 268
++Q ++GS+ RG + C G + +LG GGF ++ L + S +
Sbjct: 75 EEQLSSLEGSQGLIPSRGWTDDLCYGTGAVYLLGLGIGGFSGMMQGLQNIPPNSPGKLQL 134
Query: 269 SYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTNILAATTTGLIHGFGSL 448
+ +++ I K + G + L Y +S + + D +I A TG + F S
Sbjct: 135 NTVLNHITKRGPFLGNNAGILALSYNIINSTIDALRGKHDTAGSIGAGALTGAL--FKSS 192
Query: 449 SWQRAIKGGGVGLAVGCAI 505
+ + +A CA+
Sbjct: 193 KGLKPMGYSSAMVAAACAV 211
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,388,123
Number of Sequences: 369166
Number of extensions: 1243538
Number of successful extensions: 3575
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3366
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3559
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6219306880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)