Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_E18
(468 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q7T2P6|TIM23_BRARE Mitochondrial import inner membrane t... 47 3e-05
sp|O14925|TIM23_HUMAN Mitochondrial import inner membrane t... 44 1e-04
sp|O35093|TIM23_RAT Mitochondrial import inner membrane tra... 44 1e-04
sp|Q9WTQ8|TIM23_MOUSE Mitochondrial import inner membrane t... 44 2e-04
sp|Q12328|TIM22_YEAST Mitochondrial import inner membrane t... 33 0.25
sp|P87146|TIM22_SCHPO Mitochondrial import inner membrane t... 33 0.42
sp|O67178|YA88_AQUAE Hypothetical protein AQ_1088 31 1.2
sp|Q6N5Q9|LPXD_RHOPA UDP-3-O-[3-hydroxymyristoyl] glucosami... 29 4.6
sp|Q7Z3B4|NUP54_HUMAN Nucleoporin p54 (54 kDa nucleoporin) 29 4.6
sp|O83986|RECX_TREPA Regulatory protein recX 28 7.9
>sp|Q7T2P6|TIM23_BRARE Mitochondrial import inner membrane translocase subunit Tim23
Length = 208
Score = 46.6 bits (109), Expect = 3e-05
Identities = 31/123 (25%), Positives = 59/123 (47%), Gaps = 8/123 (6%)
Frame = +2
Query: 5 ILGGAYGGFKSIIPAL-------WSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAG 163
I G A+G + L WS+ +++ + + A++A +LG + LLY+
Sbjct: 85 ITGAAFGTLNGLRMGLSETRDMPWSKP----RNVQILNMVTRQGASWANTLGSVALLYSV 140
Query: 164 CDSILHYSLKQEDEVTNILAATTTGLIHGFGSLSWQRAI-KGGGVGLAVGCAIGAFSNYD 340
+ + ED++ + A T TG++ F S + + +GG +GLA+ ++N+D
Sbjct: 141 FGVAIEKARGAEDDLNTVAAGTLTGMV--FKSTGGLKGVARGGLIGLAMSGLYALYNNWD 198
Query: 341 KLK 349
LK
Sbjct: 199 HLK 201
>sp|O14925|TIM23_HUMAN Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 44.3 bits (103), Expect = 1e-04
Identities = 26/115 (22%), Positives = 56/115 (48%), Gaps = 1/115 (0%)
Frame = +2
Query: 14 GAYGGFKSIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLK 193
GA G + + + + + +++ + + A +A +LG + LLY+ I+ +
Sbjct: 91 GAMNGLRLGLKETQNMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRG 150
Query: 194 QEDEVTNILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 355
ED++ + A T TG+++ G L + +GG GL + ++N++ +K S
Sbjct: 151 AEDDLNTVAAGTMTGMLYKCTGGL--RGIARGGLTGLTLTSLYALYNNWEHMKGS 203
>sp|O35093|TIM23_RAT Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 44.3 bits (103), Expect = 1e-04
Identities = 27/115 (23%), Positives = 54/115 (46%), Gaps = 1/115 (0%)
Frame = +2
Query: 14 GAYGGFKSIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLK 193
GA G + + S + +++ + + A +A +LG + LLY+ I+ +
Sbjct: 91 GALNGLRLGLKETQSMPWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRG 150
Query: 194 QEDEVTNILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 355
ED+ + A T TG+++ G L + +GG GL + ++N++ +K S
Sbjct: 151 AEDDFNTVAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSVYALYNNWEHMKGS 203
>sp|Q9WTQ8|TIM23_MOUSE Mitochondrial import inner membrane translocase subunit Tim23
Length = 209
Score = 43.5 bits (101), Expect = 2e-04
Identities = 27/115 (23%), Positives = 55/115 (47%), Gaps = 1/115 (0%)
Frame = +2
Query: 14 GAYGGFKSIIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCDSILHYSLK 193
GA G + + S + + +++ + + A +A +LG + LLY+ I+ +
Sbjct: 91 GAMNGLRLGLKETQSMAWSKPRNVQILNMVTRQGALWANTLGSLALLYSAFGVIIEKTRG 150
Query: 194 QEDEVTNILAATTTGLIHG-FGSLSWQRAIKGGGVGLAVGCAIGAFSNYDKLKSS 355
ED++ + A T TG+++ G L + +GG GL + ++N+ +K S
Sbjct: 151 AEDDLNTVAAGTMTGMLYKCTGGL--RGIARGGLAGLTLTSLYALYNNWVHMKGS 203
>sp|Q12328|TIM22_YEAST Mitochondrial import inner membrane translocase subunit TIM22
Length = 207
Score = 33.5 bits (75), Expect = 0.25
Identities = 24/81 (29%), Positives = 43/81 (53%), Gaps = 3/81 (3%)
Frame = +2
Query: 95 SQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTNILAA---TTTGLIHGFGSLS 265
+ + K S + A++ G IG++YAG + ++ SL+ ++++ N + A T GL + G
Sbjct: 116 TDMGKKSYSSAKNFGYIGMIYAGVECVIE-SLRAKNDIYNGVTAGFFTGAGLAYKAGP-- 172
Query: 266 WQRAIKGGGVGLAVGCAIGAF 328
Q A+ GG A AI +
Sbjct: 173 -QAALMGGAGFAAFSAAIDLY 192
>sp|P87146|TIM22_SCHPO Mitochondrial import inner membrane translocase subunit tim22
Length = 175
Score = 32.7 bits (73), Expect = 0.42
Identities = 30/106 (28%), Positives = 47/106 (44%), Gaps = 6/106 (5%)
Frame = +2
Query: 8 LGGAYGGFKS------IIPALWSRSLREANRSYLISQIAKSSATFAQSLGGIGLLYAGCD 169
LGG +G F S I P ++ + RE R ++S +T A++ G +GL+Y+G +
Sbjct: 60 LGGIFGMFISSLDLQHIDPKIYEKPFREQIRIQARDMGSRSFST-AKNFGLLGLIYSGSE 118
Query: 170 SILHYSLKQEDEVTNILAATTTGLIHGFGSLSWQRAIKGGGVGLAV 307
+ + D I A TG S +AI GG G +
Sbjct: 119 CCIEAFRAKTDIYNAIAAGVFTG--GALAVRSGPKAIVLGGAGFGL 162
>sp|O67178|YA88_AQUAE Hypothetical protein AQ_1088
Length = 761
Score = 31.2 bits (69), Expect = 1.2
Identities = 24/75 (32%), Positives = 37/75 (49%), Gaps = 11/75 (14%)
Frame = +2
Query: 89 LISQIAKSSATFAQSLGGIGLLY------AGCDSILHYSLKQEDEVTNILAATTTGLIHG 250
L+SQ++++S AQ L +GLLY + +L +LK D V + L A L +
Sbjct: 57 LLSQVSENSLNSAQGLSDLGLLYFFLGRVEDAERVLKKALKFSD-VDDALYARLGALYYS 115
Query: 251 FGSLS-----WQRAI 280
G L W+RA+
Sbjct: 116 QGKLEEAQHYWERAL 130
>sp|Q6N5Q9|LPXD_RHOPA UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
Length = 360
Score = 29.3 bits (64), Expect = 4.6
Identities = 21/66 (31%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = +2
Query: 140 GIGLLYAGCDSILHYSLKQEDEVT-NILAATTTGLIHGFGSLSWQRAIKGGGVGLAVGCA 316
G+G +++H + K EDEVT LA + G G++ A+ GV + C
Sbjct: 113 GVGAPGIAPTAVIHETAKLEDEVTVEPLAVIGPDVEIGSGTVIGAGAVIAAGVKIGRDCD 172
Query: 317 IGAFSN 334
IGA S+
Sbjct: 173 IGAGSH 178
>sp|Q7Z3B4|NUP54_HUMAN Nucleoporin p54 (54 kDa nucleoporin)
Length = 507
Score = 29.3 bits (64), Expect = 4.6
Identities = 23/94 (24%), Positives = 35/94 (37%), Gaps = 3/94 (3%)
Frame = +2
Query: 95 SQIAKSSATFAQSLGGIGLLYAGCDSILHYSLKQEDEVTNILAATTT---GLIHGFGSLS 265
S A ++A A GG G S +S T + T G GFG+ +
Sbjct: 12 SGTAAATAAPAGGFGGFGTTSTTAGSAFSFSAPTNTGTTGLFGGTQNKGFGFGTGFGTTT 71
Query: 266 WQRAIKGGGVGLAVGCAIGAFSNYDKLKSSFRGM 367
G G GL G G F+ + +++ G+
Sbjct: 72 GTST--GLGTGLGTGLGFGGFNTQQQQQTTLGGL 103
>sp|O83986|RECX_TREPA Regulatory protein recX
Length = 164
Score = 28.5 bits (62), Expect = 7.9
Identities = 25/91 (27%), Positives = 44/91 (48%), Gaps = 7/91 (7%)
Frame = +2
Query: 32 KSIIPAL----WSRSLREANRSYLISQIAKSSATFAQSLGGI---GLLYAGCDSILHYSL 190
+S+ P L W R A R++L +++ A+ AQ LG + G+ +A + L
Sbjct: 55 ESVFPVLKRYSWLDDTRFA-RAWLRNRVDSRPASRAQLLGELKRRGVSHADAEGALDLLF 113
Query: 191 KQEDEVTNILAATTTGLIHGFGSLSWQRAIK 283
+++DE T L G+G + QRA++
Sbjct: 114 QEQDEETLCLRLLEKLRRRGYGPHTLQRALQ 144
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,689,896
Number of Sequences: 369166
Number of extensions: 820529
Number of successful extensions: 2575
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2398
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2564
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2683624230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)