Planaria EST Database

DrC_01045

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01045
         (1022 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q94547|RGA_DROME  Regulator of gene activity (Regena prot...    82   3e-15
sp|Q9NZN8|CNOT2_HUMAN  CCR4-NOT transcription complex subuni...    75   3e-13
sp|Q8C5L3|CNOT2_MOUSE  CCR4-NOT transcription complex subuni...    75   3e-13
sp|O75175|CNOT3_HUMAN  CCR4-NOT transcription complex subuni...    63   2e-09
sp|Q8K0V4|CNOT3_MOUSE  CCR4-NOT transcription complex subuni...    63   2e-09
sp|P06100|NOT2_YEAST  General negative regulator of transcri...    55   2e-07
sp|Q12514|NOT5_YEAST  General negative regulator of transcri...    40   0.014
sp|P04936|POLG_HRV2  Genome polyprotein [Contains: Coat prot...    35   0.26 
sp|O43918|AIRE_HUMAN  Autoimmune regulator (Autoimmune polye...    34   0.59 
sp|P36304|POLR_KYMVJ  RNA replicase polyprotein                    32   2.9  

>sp|Q94547|RGA_DROME Regulator of gene activity (Regena protein)
          Length = 585

 Score = 81.6 bits (200), Expect = 3e-15
 Identities = 38/109 (34%), Positives = 68/109 (62%)
 Frame = +2

Query: 20  RPHDMDYAVPKEYRIRHSILDKLADPLPEKLSDETLFWIFYNLTGEECQTTAAKQLMARQ 199
           R  D+++ VP EY I  +I DKL  P+ +KL ++ LF++FY   G+  Q  AA +L +R+
Sbjct: 458 RAQDVEFNVPPEYLINFAIRDKLTAPVLKKLQEDLLFFLFYTNIGDMMQLMAAAELHSRE 517

Query: 200 WRYNKRDKFWLTRDSKGVNEPTVHPNLERGSYIVWDYEKAQRVTKILTV 346
           WRY+  +K W+TR   G+++   +   ERG++  +D +  +R++K+  +
Sbjct: 518 WRYHVEEKIWITR-IPGIDQYEKNGTKERGTFYYFDAQSWKRLSKVFQI 565

>sp|Q9NZN8|CNOT2_HUMAN CCR4-NOT transcription complex subunit 2 (CCR4-associated factor 2)
          Length = 540

 Score = 75.1 bits (183), Expect = 3e-13
 Identities = 44/116 (37%), Positives = 66/116 (56%), Gaps = 1/116 (0%)
 Frame = +2

Query: 20  RPHDMDYAVPKEYRIRHSILDKLADPLPEKLSDETLFWIFYNLTGEECQTTAAKQLMARQ 199
           RP D+D+ VP EY     I DKLA     +  ++ LF+++Y   G+  Q  AA +L  R 
Sbjct: 409 RPQDIDFHVPSEYLTNIHIRDKLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRD 468

Query: 200 WRYNKRDKFWLTRDSKGVNEPTVHPN-LERGSYIVWDYEKAQRVTKILTVYYKDLE 364
           WRY+K ++ W+TR + G+ EPT+  N  ERG+Y  +D    ++V K   + Y  LE
Sbjct: 469 WRYHKEERVWITR-APGM-EPTMKTNTYERGTYYFFDCLNWRKVAKEFHLEYDKLE 522

>sp|Q8C5L3|CNOT2_MOUSE CCR4-NOT transcription complex subunit 2 (CCR4-associated factor 2)
          Length = 540

 Score = 75.1 bits (183), Expect = 3e-13
 Identities = 44/116 (37%), Positives = 66/116 (56%), Gaps = 1/116 (0%)
 Frame = +2

Query: 20  RPHDMDYAVPKEYRIRHSILDKLADPLPEKLSDETLFWIFYNLTGEECQTTAAKQLMARQ 199
           RP D+D+ VP EY     I DKLA     +  ++ LF+++Y   G+  Q  AA +L  R 
Sbjct: 409 RPQDIDFHVPSEYLTNIHIRDKLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRD 468

Query: 200 WRYNKRDKFWLTRDSKGVNEPTVHPN-LERGSYIVWDYEKAQRVTKILTVYYKDLE 364
           WRY+K ++ W+TR + G+ EPT+  N  ERG+Y  +D    ++V K   + Y  LE
Sbjct: 469 WRYHKEERVWITR-APGM-EPTMKTNTYERGTYYFFDCLNWRKVAKEFHLEYDKLE 522

>sp|O75175|CNOT3_HUMAN CCR4-NOT transcription complex subunit 3 (CCR4-associated factor 3)
          Length = 753

 Score = 62.8 bits (151), Expect = 2e-09
 Identities = 35/89 (39%), Positives = 51/89 (57%), Gaps = 1/89 (1%)
 Frame = +2

Query: 104 EKLSDETLFWIFYNLTGEECQTTAAKQLMARQWRYNKRDKFWLTRDSKGVNEPTVHPNLE 283
           ++LS ETLF+IFY L G + Q  AAK L  + WR++ +   W  R  +     T+    E
Sbjct: 664 QRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEE---PKTITDEFE 720

Query: 284 RGSYIVWDYEK-AQRVTKILTVYYKDLEN 367
           +G+YI +DYEK  QR  +  T  Y+ LE+
Sbjct: 721 QGTYIYFDYEKWGQRKKEGFTFEYRYLED 749

>sp|Q8K0V4|CNOT3_MOUSE CCR4-NOT transcription complex subunit 3 (CCR4-associated factor 3)
          Length = 751

 Score = 62.8 bits (151), Expect = 2e-09
 Identities = 35/89 (39%), Positives = 51/89 (57%), Gaps = 1/89 (1%)
 Frame = +2

Query: 104 EKLSDETLFWIFYNLTGEECQTTAAKQLMARQWRYNKRDKFWLTRDSKGVNEPTVHPNLE 283
           ++LS ETLF+IFY L G + Q  AAK L  + WR++ +   W  R  +     T+    E
Sbjct: 662 QRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEE---PKTITDEFE 718

Query: 284 RGSYIVWDYEK-AQRVTKILTVYYKDLEN 367
           +G+YI +DYEK  QR  +  T  Y+ LE+
Sbjct: 719 QGTYIYFDYEKWGQRKKEGFTFEYRYLED 747

>sp|P06100|NOT2_YEAST General negative regulator of transcription subunit 2
          Length = 191

 Score = 55.5 bits (132), Expect = 2e-07
 Identities = 30/81 (37%), Positives = 46/81 (56%), Gaps = 2/81 (2%)
 Frame = +2

Query: 116 DETLFWIFYNLTGEECQTTAAKQLMARQWRYNKRDKFWLTRDSKGVNEPTVHPN--LERG 289
           DETLF++FY   G   Q     +L  R WRY+K  K WLT+D   + EP V  +   ERG
Sbjct: 109 DETLFFLFYKHPGTVIQELTYLELRKRNWRYHKTLKAWLTKDP--MMEPIVSADGLSERG 166

Query: 290 SYIVWDYEKAQRVTKILTVYY 352
           SY+ +D ++ ++  +   ++Y
Sbjct: 167 SYVFFDPQRWEKCQRDFLLFY 187

>sp|Q12514|NOT5_YEAST General negative regulator of transcription subunit 5
          Length = 560

 Score = 39.7 bits (91), Expect = 0.014
 Identities = 24/72 (33%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
 Frame = +2

Query: 107 KLSDETLFWIFYNLTGEECQTTAAKQLMA-RQWRYNKRDKFWLTRDSKGVNEPTVHPNLE 283
           K   +TLF+IFY+  G   Q  AA++L   R W +NK D+ W  ++ + +  P      E
Sbjct: 471 KFDLDTLFFIFYHYQGSYEQFLAARELFKNRNWLFNKVDRCWYYKEIEKL--PPGMGKSE 528

Query: 284 RGSYIVWDYEKA 319
             S+  +DY+K+
Sbjct: 529 EESWRYFDYKKS 540

>sp|P04936|POLG_HRV2 Genome polyprotein [Contains: Coat protein VP4 (P1A); Coat protein
           VP2 (P1B); Coat protein VP3 (P1C); Coat protein VP1
           (P1D); Picornain 2A (Core protein P2A); Core protein
           P2B; Core protein P2C; Core protein P3A; Genome-linked
           protein VPg (P3B); Picornain 3C (Protease 3C) (P3C);
           RNA-directed RNA polymerase (P3D)]
          Length = 2150

 Score = 35.4 bits (80), Expect = 0.26
 Identities = 21/93 (22%), Positives = 38/93 (40%), Gaps = 1/93 (1%)
 Frame = +2

Query: 209 NKRDKFWLTRDSKGVNEPTVHPNLERGSYIVWDYEKAQRVTKILT-VYYKDLENVTPATT 385
           N   K  L     G+ EPT   +   G++++WD      ++ ++  +      N +P  +
Sbjct: 454 NTTVKLLLAYTPPGIAEPTTRKDAMLGTHVIWDVGLQSTISMVVPWISASHYRNTSPGRS 513

Query: 386 KSLFPFCYNSPFIKRSAVNSSAIPPPQLPPTGR 484
            S +  C+           +  + PPQ PPT R
Sbjct: 514 TSGYITCW---------YQTRLVIPPQTPPTAR 537

>sp|O43918|AIRE_HUMAN Autoimmune regulator (Autoimmune polyendocrinopathy candidiasis
           ectodermal dystrophy protein) (APECED protein)
          Length = 545

 Score = 34.3 bits (77), Expect = 0.59
 Identities = 47/200 (23%), Positives = 86/200 (43%)
 Frame = +2

Query: 41  AVPKEYRIRHSILDKLADPLPEKLSDETLFWIFYNLTGEECQTTAAKQLMARQWRYNKRD 220
           AV   + + H++ D   D +PE    ETL     +L  +E    A   L++         
Sbjct: 21  AVDSAFPLLHALADH--DVVPEDKFQETL-----HLKEKEGCPQAFHALLS--------- 64

Query: 221 KFWLTRDSKGVNEPTVHPNLERGSYIVWDYEKAQRVTKILTVYYKDLENVTPATTKSLFP 400
            + LT+DS  + +        R  +  ++ E+  R+  IL  + KD++   P   +    
Sbjct: 65  -WLLTQDSTAILD------FWRVLFKDYNLERYGRLQPILDSFPKDVDLSQPRKGR---- 113

Query: 401 FCYNSPFIKRSAVNSSAIPPPQLPPTGRTNQKNLATFLQNPSAAASVEARLLNNPISQNF 580
                   K  AV  + +PPP+LP   + +++  A      +A A++  R   +P SQ  
Sbjct: 114 --------KPPAVPKALVPPPRLPTKRKASEEARA------AAPAALTPRGTASPGSQLK 159

Query: 581 SQPDQMMTSTASQIRVQINN 640
           ++P +   S+A Q R+ + N
Sbjct: 160 AKPPKKPESSAEQQRLPLGN 179

>sp|P36304|POLR_KYMVJ RNA replicase polyprotein
          Length = 1874

 Score = 32.0 bits (71), Expect = 2.9
 Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 6/75 (8%)
 Frame = +2

Query: 410 NSPFIKRSAVNSSAIPPPQLPPTGRTNQKNLATFLQNPSAAASVEARLLNNP------IS 571
           +SPF+    +N SA+PPPQ  PT  T      + L  P     V++ L+ +P      +S
Sbjct: 722 HSPFLSDGQLNYSALPPPQ-DPTNTT-----LSLLPEPKPPTEVQSPLMADPTCVGPAVS 775

Query: 572 QNFSQPDQMMTSTAS 616
            +   P     +TAS
Sbjct: 776 FSSLYPRDFFPNTAS 790

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 107,919,087
Number of Sequences: 369166
Number of extensions: 2144907
Number of successful extensions: 5640
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5382
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5631
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10957511455
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01045

  1. Dr_sW_026_F10
  2. Dr_sW_009_F21
  3. Dr_sW_024_I17
  4. Dr_sW_022_M11
  5. Dr_sW_015_N01