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Planaria EST Database

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01012
         (515 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P46821|MAP1B_HUMAN  Microtubule-associated protein 1B (MA...    49   7e-06
sp|P14873|MAP1B_MOUSE  Microtubule-associated protein 1B (MA...    48   2e-05
sp|P15205|MAP1B_RAT  Microtubule-associated protein 1B (MAP ...    45   8e-05
sp|Q9I954|TYBB_CYPCA  Thymosin beta-b                              44   3e-04
sp|P21752|TYB9_BOVIN  Thymosin beta-9 (Thymosin beta-10) [Co...    43   5e-04
sp|P02829|HSP82_YEAST  ATP-dependent molecular chaperone HSP...    42   7e-04
sp|P21753|TYB9_PIG  Thymosin beta-9                                42   9e-04
sp|P33248|TYB12_LATJA  Thymosin beta-12                            40   0.003
sp|P63312|TYB10_RAT  Thymosin beta-10 >gi|54036496|sp|Q6ZWY8...    40   0.003
sp|Q59037|SMC_METJA  Chromosome partition protein smc homolog      40   0.003

>sp|P46821|MAP1B_HUMAN Microtubule-associated protein 1B (MAP 1B) [Contains: MAP1 light
           chain LC1]
          Length = 2468

 Score = 48.9 bits (115), Expect = 7e-06
 Identities = 36/121 (29%), Positives = 58/121 (47%)
 Frame = +3

Query: 45  SIMSETAETKLATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEK 224
           S+  +   +K   SPV  EV E Q   +K   A+EK V       K+ K + E K E ++
Sbjct: 601 SVTEKEVPSKEEPSPVKAEVAEKQATDVKPKAAKEKTVK------KETKVKPEDKKEEKE 654

Query: 225 GTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITKEHELRPVTTEVKQRLPSQIDIEAE 404
             K +  +  +KT +  EE  KKE+ + ++  EI KE +  P     K+  P ++  E +
Sbjct: 655 KPKKEVAKKEDKTPIKKEEKPKKEEVKKEVKKEIKKEEKKEPKKEVKKETPPKEVKKEVK 714

Query: 405 K 407
           K
Sbjct: 715 K 715

 Score = 38.9 bits (89), Expect = 0.007
 Identities = 30/140 (21%), Positives = 60/140 (42%), Gaps = 6/140 (4%)
 Frame = +3

Query: 6   GTRIFSYLRKFNKSIMSETAETKLATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQ 185
           G     +L    + + ++   T    +PVV +       KLK      + + P    +  
Sbjct: 501 GLEKLKHLDFLKQPLATQKDLTGQVPTPVVKQT------KLKQRADSRESLKPAAKPLPS 554

Query: 186 EKTEQELKDEIEKGTKLKHV------ETTEKTVLPNEEDIKKEKQEAQLLTEITKEHELR 347
           +   +E K+E  + TK+ HV      E+ EK ++  ++ +K E + +    E+  + E  
Sbjct: 555 KSVRKESKEETPEVTKVNHVEKPPKVESKEKVMVKKDKPVKTETKPSVTEKEVPSKEEPS 614

Query: 348 PVTTEVKQRLPSQIDIEAEK 407
           PV  EV ++  + +  +A K
Sbjct: 615 PVKAEVAEKQATDVKPKAAK 634

 Score = 37.0 bits (84), Expect = 0.028
 Identities = 24/112 (21%), Positives = 58/112 (51%), Gaps = 2/112 (1%)
 Frame = +3

Query: 42  KSIMSETAETKLATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIE 221
           K+   +T + +    P  ++  E +K K +  + ++K  +   +  K+E+ ++E+K EI+
Sbjct: 631 KAAKEKTVKKETKVKPE-DKKEEKEKPKKEVAKKEDKTPIKKEEKPKKEEVKKEVKKEIK 689

Query: 222 KGTKLKHVETTEKTVLPNE--EDIKKEKQEAQLLTEITKEHELRPVTTEVKQ 371
           K  K +  +  +K   P E  +++KKE+++     E   + E++ +  + K+
Sbjct: 690 KEEKKEPKKEVKKETPPKEVKKEVKKEEKKEVKKEEKEPKKEIKKLPKDAKK 741

>sp|P14873|MAP1B_MOUSE Microtubule-associated protein 1B (MAP 1B) (MAP1.2) (MAP1(X))
           [Contains: MAP1 light chain LC1]
          Length = 2464

 Score = 47.8 bits (112), Expect = 2e-05
 Identities = 36/128 (28%), Positives = 62/128 (48%), Gaps = 7/128 (5%)
 Frame = +3

Query: 45  SIMSETAETKLATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEK 224
           S+  +   +K   SPV  EV E Q  + K    ++KVV  +  T  +EK E++ K E+ K
Sbjct: 601 SVTEKEVSSKEEQSPVKAEVAEKQATESKPKVTKDKVVKKEIKTKLEEKKEEKPKKEVVK 660

Query: 225 GTKLKHVETTEKTVLPNEEDIKKE-------KQEAQLLTEITKEHELRPVTTEVKQRLPS 383
                 ++  EK   P +E++KKE       ++  +L  E+ KE  L+    EVK+    
Sbjct: 661 KEDKTPLKKDEK---PRKEEVKKEIKKEIKKEERKELKKEVKKETPLKDAKKEVKKEEKK 717

Query: 384 QIDIEAEK 407
           ++  E ++
Sbjct: 718 EVKKEEKE 725

 Score = 35.4 bits (80), Expect = 0.081
 Identities = 22/90 (24%), Positives = 41/90 (45%), Gaps = 6/90 (6%)
 Frame = +3

Query: 123 KLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETT------EKTVLPNEED 284
           KLK      + + P    +  +   +E K+E  + TK   VE T      EK ++  ++ 
Sbjct: 534 KLKQRADSRESLKPATKPVASKSVRKESKEETPEVTKTSQVEKTPKVESKEKVLVKKDKP 593

Query: 285 IKKEKQEAQLLTEITKEHELRPVTTEVKQR 374
           +K E + +    E++ + E  PV  EV ++
Sbjct: 594 VKTESKPSVTEKEVSSKEEQSPVKAEVAEK 623

>sp|P15205|MAP1B_RAT Microtubule-associated protein 1B (MAP 1B) (Neuraxin) [Contains:
           MAP1 light chain LC1]
          Length = 2459

 Score = 45.4 bits (106), Expect = 8e-05
 Identities = 35/112 (31%), Positives = 55/112 (49%)
 Frame = +3

Query: 45  SIMSETAETKLATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEK 224
           S+  +   +K   SPV  EV E    + K    ++KVV  +  T  +EK E++ K E+ K
Sbjct: 600 SVTEKEVPSKEEQSPVKAEVAEKAATESKPKVTKDKVVKKEIKTKPEEKKEEKPKKEVAK 659

Query: 225 GTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITKEHELRPVTTEVKQRLP 380
                     +KT L  +E  KKE+ + ++  EI KE E + +  EVK+  P
Sbjct: 660 --------KEDKTPLKKDEKPKKEEAKKEIKKEIKKE-EKKELKKEVKKETP 702

 Score = 34.7 bits (78), Expect = 0.14
 Identities = 28/133 (21%), Positives = 55/133 (41%), Gaps = 6/133 (4%)
 Frame = +3

Query: 6   GTRIFSYLRKFNKSIMSETAETKLATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQ 185
           G     +L    + + ++   T   ++P V +V      KLK      + + P    +  
Sbjct: 500 GLEKLKHLDFLKQPLATQKDLTGQVSTPPVKQV------KLKQRADSRESLKPATKPLSS 553

Query: 186 EKTEQELKDEIEKGTKLKHVETT------EKTVLPNEEDIKKEKQEAQLLTEITKEHELR 347
           +   +E K+E  + TK   VE T      EK ++  ++  K E + +    E+  + E  
Sbjct: 554 KSVRKESKEEAPEATKASQVEKTPKVESKEKVIVKKDKPGKVESKPSVTEKEVPSKEEQS 613

Query: 348 PVTTEVKQRLPSQ 386
           PV  EV ++  ++
Sbjct: 614 PVKAEVAEKAATE 626

>sp|Q9I954|TYBB_CYPCA Thymosin beta-b
          Length = 43

 Score = 43.5 bits (101), Expect = 3e-04
 Identities = 20/39 (51%), Positives = 27/39 (69%)
 Frame = +3

Query: 75  LATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQEK 191
           +A  P ++EV++F K KLK  E QEK  LP  +TI+QEK
Sbjct: 1   MADKPDISEVSQFDKTKLKKTETQEKNTLPTKETIEQEK 39

 Score = 33.1 bits (74), Expect = 0.40
 Identities = 15/25 (60%), Positives = 18/25 (72%)
 Frame = +3

Query: 228 TKLKHVETTEKTVLPNEEDIKKEKQ 302
           TKLK  ET EK  LP +E I++EKQ
Sbjct: 16  TKLKKTETQEKNTLPTKETIEQEKQ 40

>sp|P21752|TYB9_BOVIN Thymosin beta-9 (Thymosin beta-10) [Contains: Thymosin beta-8]
          Length = 42

 Score = 42.7 bits (99), Expect = 5e-04
 Identities = 20/39 (51%), Positives = 25/39 (64%)
 Frame = +3

Query: 75  LATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQEK 191
           +A  P + E+N F K KLK  E QEK  LP  +TI+QEK
Sbjct: 1   MADKPDLGEINSFDKAKLKKTETQEKNTLPTKETIEQEK 39

 Score = 31.2 bits (69), Expect = 1.5
 Identities = 14/24 (58%), Positives = 17/24 (70%)
 Frame = +3

Query: 231 KLKHVETTEKTVLPNEEDIKKEKQ 302
           KLK  ET EK  LP +E I++EKQ
Sbjct: 17  KLKKTETQEKNTLPTKETIEQEKQ 40

>sp|P02829|HSP82_YEAST ATP-dependent molecular chaperone HSP82 (Heat shock protein Hsp90
           heat inducible isoform) (82 kDa heat shock protein)
          Length = 709

 Score = 42.4 bits (98), Expect = 7e-04
 Identities = 33/109 (30%), Positives = 54/109 (49%), Gaps = 3/109 (2%)
 Frame = +3

Query: 27  LRKFNKSIMSETAETKLATSPVVNEVNEFQKDKLKHV---EAQEKVVLPDHDTIKQEKTE 197
           LR F K    E  E K     V+   +EF    ++ V   E +++V +P+ +   +EK +
Sbjct: 173 LRLFLKDDQLEYLEEK-RIKEVIKRHSEFVAYPIQLVVTKEVEKEVPIPEEEKKDEEKKD 231

Query: 198 QELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITKEHEL 344
           +E KDE +K  KL+ V+  E+     +   KK K+E Q + E+ K   L
Sbjct: 232 EEKKDEDDKKPKLEEVDEEEE----KKPKTKKVKEEVQEIEELNKTKPL 276

>sp|P21753|TYB9_PIG Thymosin beta-9
          Length = 41

 Score = 42.0 bits (97), Expect = 9e-04
 Identities = 20/38 (52%), Positives = 24/38 (63%)
 Frame = +3

Query: 78  ATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQEK 191
           A  P + E+N F K KLK  E QEK  LP  +TI+QEK
Sbjct: 1   ADKPDMGEINSFDKAKLKKTETQEKNTLPTKETIEQEK 38

 Score = 31.2 bits (69), Expect = 1.5
 Identities = 14/24 (58%), Positives = 17/24 (70%)
 Frame = +3

Query: 231 KLKHVETTEKTVLPNEEDIKKEKQ 302
           KLK  ET EK  LP +E I++EKQ
Sbjct: 16  KLKKTETQEKNTLPTKETIEQEKQ 39

>sp|P33248|TYB12_LATJA Thymosin beta-12
          Length = 43

 Score = 40.0 bits (92), Expect = 0.003
 Identities = 19/35 (54%), Positives = 23/35 (65%)
 Frame = +3

Query: 87  PVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQEK 191
           P ++EV  F K KLK  E QEK  LP  +TI+QEK
Sbjct: 4   PDISEVTSFDKTKLKKTETQEKNPLPSKETIEQEK 38

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 15/27 (55%), Positives = 19/27 (70%)
 Frame = +3

Query: 228 TKLKHVETTEKTVLPNEEDIKKEKQEA 308
           TKLK  ET EK  LP++E I++EK  A
Sbjct: 15  TKLKKTETQEKNPLPSKETIEQEKAAA 41

>sp|P63312|TYB10_RAT Thymosin beta-10
 sp|Q6ZWY8|TYB10_MOUSE Thymosin beta-10
 sp|P63313|TYB10_HUMAN Thymosin beta-10
 sp|P63314|TYB10_HORSE Thymosin beta-10
          Length = 44

 Score = 40.0 bits (92), Expect = 0.003
 Identities = 19/42 (45%), Positives = 25/42 (59%)
 Frame = +3

Query: 75  LATSPVVNEVNEFQKDKLKHVEAQEKVVLPDHDTIKQEKTEQ 200
           +A  P + E+  F K KLK  E QEK  LP  +TI+QEK  +
Sbjct: 1   MADKPDMGEIASFDKAKLKKTETQEKNTLPTKETIEQEKRSE 42

>sp|Q59037|SMC_METJA Chromosome partition protein smc homolog
          Length = 1169

 Score = 40.0 bits (92), Expect = 0.003
 Identities = 33/139 (23%), Positives = 64/139 (46%), Gaps = 16/139 (11%)
 Frame = +3

Query: 39   NKSIMSETAETKLATSPVVNEVNEFQKDKLKHVEAQEKVV--LPDHDTIKQ--------- 185
            NK I+ E  E  L    ++N +NE +    + +E +EK++  L ++++ +          
Sbjct: 748  NKDILEELEELNLKREEILNRINEIESKINELIERREKIINELKEYESDENLKRMNEIEG 807

Query: 186  -----EKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITKEHELRP 350
                 EK + +LK+EI+KG  L      ++ ++P  E++ K+  E      I K+  L  
Sbjct: 808  ELKILEKEKAKLKNEIDKGLTL-----VKEILIPKIEELNKKVSEL-----INKKVILEK 857

Query: 351  VTTEVKQRLPSQIDIEAEK 407
              +  K+ +   + I  EK
Sbjct: 858  NISFYKESIEKNLSILEEK 876

 Score = 37.7 bits (86), Expect = 0.016
 Identities = 26/100 (26%), Positives = 52/100 (52%), Gaps = 3/100 (3%)
 Frame = +3

Query: 42  KSIMSETAETKLATSPVVNEVNEFQKDKLK--HVEAQEKVVLPDHDTIKQEKTEQELKD- 212
           + I  E    KL  + ++NE+NE   +++   H   +E  V  ++D    + +  ELK  
Sbjct: 267 REIDVEIENLKLRLNNIINELNEKGNEEVLELHKSIKELEVEIENDKKVLDSSINELKKV 326

Query: 213 EIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITK 332
           E+E   K K ++ T+K ++ N + I +++Q+ + + E  K
Sbjct: 327 EVEIENKKKEIKETQKKIIENRDSIIEKEQQIKEIEEKIK 366

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,591,878
Number of Sequences: 369166
Number of extensions: 800963
Number of successful extensions: 4624
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4090
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4491
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3354254700
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

1. Dr_sW_025_I08
2. Dr_sW_004_H09
3. Dr_sW_023_M16
4. Dr_sW_010_C13
5. Dr_sW_023_A04
6. Dr_sW_001_M12
7. Dr_sW_024_N06
8. Dr_sW_025_N07
9. Dr_sW_025_C06
10. Dr_sW_001_P09
11. Dr_sW_001_P19
12. Dr_sW_019_I01
13. Dr_sW_006_O04
14. Dr_sW_010_D06
15. Dr_sW_013_F09