Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_O04
(411 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P02829|HSP82_YEAST ATP-dependent molecular chaperone HSP... 40 0.001
sp|P46821|MAP1B_HUMAN Microtubule-associated protein 1B (MA... 40 0.002
sp|P18281|ACTO_ACACA Actobindin 39 0.005
sp|P15108|HSC82_YEAST ATP-dependent molecular chaperone HSC... 38 0.007
sp|P14873|MAP1B_MOUSE Microtubule-associated protein 1B (MA... 38 0.007
sp|P15205|MAP1B_RAT Microtubule-associated protein 1B (MAP ... 37 0.012
sp|P24709|INVO_CEBAL Involucrin 37 0.012
sp|P24724|HSP90_THEPA Heat shock protein 90 (HSP90) 37 0.020
sp|O67124|RAD50_AQUAE Probable DNA double-strand break repa... 36 0.026
sp|P21753|TYB9_PIG Thymosin beta-9 36 0.034
>sp|P02829|HSP82_YEAST ATP-dependent molecular chaperone HSP82 (Heat shock protein Hsp90
heat inducible isoform) (82 kDa heat shock protein)
Length = 709
Score = 40.4 bits (93), Expect = 0.001
Identities = 22/69 (31%), Positives = 39/69 (56%)
Frame = +3
Query: 36 EAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLL 215
E +++V +P+ + +EK ++E KDE +K KL+ V+ E+ + KK K+E Q +
Sbjct: 212 EVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEEEE----KKPKTKKVKEEVQEI 267
Query: 216 TEITKEHEL 242
E+ K L
Sbjct: 268 EELNKTKPL 276
>sp|P46821|MAP1B_HUMAN Microtubule-associated protein 1B (MAP 1B) [Contains: MAP1 light
chain LC1]
Length = 2468
Score = 39.7 bits (91), Expect = 0.002
Identities = 29/100 (29%), Positives = 48/100 (48%)
Frame = +3
Query: 6 EFQKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDI 185
E Q +K A+EK V K+ K + E K E ++ K + + +KT + EE
Sbjct: 622 EKQATDVKPKAAKEKTVK------KETKVKPEDKKEEKEKPKKEVAKKEDKTPIKKEEKP 675
Query: 186 KKEKQEAQLLTEITKEHELRPVTTEVKQRLPSQIDIEAEK 305
KKE+ + ++ EI KE + P K+ P ++ E +K
Sbjct: 676 KKEEVKKEVKKEIKKEEKKEPKKEVKKETPPKEVKKEVKK 715
Score = 38.5 bits (88), Expect = 0.005
Identities = 24/104 (23%), Positives = 49/104 (47%), Gaps = 6/104 (5%)
Frame = +3
Query: 12 QKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHV------ETTEKTVLPN 173
++ KLK + + P + + +E K+E + TK+ HV E+ EK ++
Sbjct: 531 KQTKLKQRADSRESLKPAAKPLPSKSVRKESKEETPEVTKVNHVEKPPKVESKEKVMVKK 590
Query: 174 EEDIKKEKQEAQLLTEITKEHELRPVTTEVKQRLPSQIDIEAEK 305
++ +K E + + E+ + E PV EV ++ + + +A K
Sbjct: 591 DKPVKTETKPSVTEKEVPSKEEPSPVKAEVAEKQATDVKPKAAK 634
Score = 36.2 bits (82), Expect = 0.026
Identities = 21/90 (23%), Positives = 49/90 (54%), Gaps = 2/90 (2%)
Frame = +3
Query: 6 EFQKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNE--E 179
E +K K + + ++K + + K+E+ ++E+K EI+K K + + +K P E +
Sbjct: 652 EKEKPKKEVAKKEDKTPIKKEEKPKKEEVKKEVKKEIKKEEKKEPKKEVKKETPPKEVKK 711
Query: 180 DIKKEKQEAQLLTEITKEHELRPVTTEVKQ 269
++KKE+++ E + E++ + + K+
Sbjct: 712 EVKKEEKKEVKKEEKEPKKEIKKLPKDAKK 741
>sp|P18281|ACTO_ACACA Actobindin
Length = 88
Score = 38.5 bits (88), Expect = 0.005
Identities = 22/69 (31%), Positives = 32/69 (46%)
Frame = +3
Query: 99 ELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITKEHELRPVTTEVKQRLP 278
EL+ I +G LKH ET +K+ E K+ + L E+ K HEL+ T K
Sbjct: 4 ELQSAIGQGAALKHAETVDKSAPQIENVTVKKVDRSSFLEEVAKPHELKHAETVDKSGPA 63
Query: 279 SQIDIEAEK 305
D+ +K
Sbjct: 64 IPEDVHVKK 72
Score = 33.1 bits (74), Expect = 0.22
Identities = 23/72 (31%), Positives = 39/72 (54%), Gaps = 3/72 (4%)
Frame = +3
Query: 24 LKHVEAQEKVVLPDHDTIKQEKTEQE-LKDEIEKGTKLKHVETTEKT--VLPNEEDIKKE 194
LKH E +K P + + +K ++ +E+ K +LKH ET +K+ +P + +KK
Sbjct: 15 LKHAETVDKSA-PQIENVTVKKVDRSSFLEEVAKPHELKHAETVDKSGPAIPEDVHVKKV 73
Query: 195 KQEAQLLTEITK 230
+ A L+EI K
Sbjct: 74 DRGA-FLSEIEK 84
>sp|P15108|HSC82_YEAST ATP-dependent molecular chaperone HSC82 (Heat shock protein Hsp90
constitutive isoform) (82 kDa heat shock cognate
protein)
Length = 705
Score = 38.1 bits (87), Expect = 0.007
Identities = 20/62 (32%), Positives = 37/62 (59%)
Frame = +3
Query: 36 EAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLL 215
E +++V +P+ +EK ++E KDE +K KL+ V+ E+ P + +K+E QE + L
Sbjct: 212 EVEKEVPIPE-----EEKKDEEKKDEDDKKPKLEEVDEEEEEKKPKTKKVKEEVQELEEL 266
Query: 216 TE 221
+
Sbjct: 267 NK 268
>sp|P14873|MAP1B_MOUSE Microtubule-associated protein 1B (MAP 1B) (MAP1.2) (MAP1(X))
[Contains: MAP1 light chain LC1]
Length = 2464
Score = 38.1 bits (87), Expect = 0.007
Identities = 29/107 (27%), Positives = 52/107 (48%), Gaps = 7/107 (6%)
Frame = +3
Query: 6 EFQKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDI 185
E Q + K ++KVV + T +EK E++ K E+ K ++ EK P +E++
Sbjct: 622 EKQATESKPKVTKDKVVKKEIKTKLEEKKEEKPKKEVVKKEDKTPLKKDEK---PRKEEV 678
Query: 186 KKE-------KQEAQLLTEITKEHELRPVTTEVKQRLPSQIDIEAEK 305
KKE ++ +L E+ KE L+ EVK+ ++ E ++
Sbjct: 679 KKEIKKEIKKEERKELKKEVKKETPLKDAKKEVKKEEKKEVKKEEKE 725
Score = 37.4 bits (85), Expect = 0.012
Identities = 21/91 (23%), Positives = 51/91 (56%), Gaps = 2/91 (2%)
Frame = +3
Query: 12 QKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEKGTK--LKHVETTEKTVLPNEEDI 185
+K K + V+ ++K L + ++E+ ++E+K EI+K + LK E + ++++
Sbjct: 652 EKPKKEVVKKEDKTPLKKDEKPRKEEVKKEIKKEIKKEERKELKKEVKKETPLKDAKKEV 711
Query: 186 KKEKQEAQLLTEITKEHELRPVTTEVKQRLP 278
KKE+++ E + E++ ++ ++K+ P
Sbjct: 712 KKEEKKEVKKEEKEPKKEIKKISKDIKKSTP 742
Score = 35.4 bits (80), Expect = 0.045
Identities = 22/90 (24%), Positives = 41/90 (45%), Gaps = 6/90 (6%)
Frame = +3
Query: 21 KLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETT------EKTVLPNEED 182
KLK + + P + + +E K+E + TK VE T EK ++ ++
Sbjct: 534 KLKQRADSRESLKPATKPVASKSVRKESKEETPEVTKTSQVEKTPKVESKEKVLVKKDKP 593
Query: 183 IKKEKQEAQLLTEITKEHELRPVTTEVKQR 272
+K E + + E++ + E PV EV ++
Sbjct: 594 VKTESKPSVTEKEVSSKEEQSPVKAEVAEK 623
>sp|P15205|MAP1B_RAT Microtubule-associated protein 1B (MAP 1B) (Neuraxin) [Contains:
MAP1 light chain LC1]
Length = 2459
Score = 37.4 bits (85), Expect = 0.012
Identities = 26/79 (32%), Positives = 42/79 (53%)
Frame = +3
Query: 42 QEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTE 221
++KVV + T +EK E++ K E+ K +KT L +E KKE+ + ++ E
Sbjct: 633 KDKVVKKEIKTKPEEKKEEKPKKEVAK--------KEDKTPLKKDEKPKKEEAKKEIKKE 684
Query: 222 ITKEHELRPVTTEVKQRLP 278
I KE E + + EVK+ P
Sbjct: 685 IKKE-EKKELKKEVKKETP 702
Score = 33.9 bits (76), Expect = 0.13
Identities = 22/94 (23%), Positives = 41/94 (43%), Gaps = 6/94 (6%)
Frame = +3
Query: 21 KLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETT------EKTVLPNEED 182
KLK + + P + + +E K+E + TK VE T EK ++ ++
Sbjct: 533 KLKQRADSRESLKPATKPLSSKSVRKESKEEAPEATKASQVEKTPKVESKEKVIVKKDKP 592
Query: 183 IKKEKQEAQLLTEITKEHELRPVTTEVKQRLPSQ 284
K E + + E+ + E PV EV ++ ++
Sbjct: 593 GKVESKPSVTEKEVPSKEEQSPVKAEVAEKAATE 626
>sp|P24709|INVO_CEBAL Involucrin
Length = 428
Score = 37.4 bits (85), Expect = 0.012
Identities = 33/95 (34%), Positives = 53/95 (55%), Gaps = 7/95 (7%)
Frame = +3
Query: 6 EFQKDKLKHVEAQEK-VVLPDHDTIKQ---EKTEQELKDEIEKGTKLKHVETTE-KTVLP 170
E Q+ +LKH+E QEK + LP+ + + E+ E +LK E+ +LKH+E E + LP
Sbjct: 157 EQQEGQLKHLEQQEKPLELPEQQSGQPKYLEQQEGQLKHLEEQKGQLKHLEQQEGQLELP 216
Query: 171 NEEDIKK--EKQEAQLLTEITKEHELRPVTTEVKQ 269
+ D K E+ E QL +E +L+ + E +Q
Sbjct: 217 EQVDQPKHLEQLEKQLEHPEQQEGKLKKLEEEEEQ 251
>sp|P24724|HSP90_THEPA Heat shock protein 90 (HSP90)
Length = 721
Score = 36.6 bits (83), Expect = 0.020
Identities = 25/88 (28%), Positives = 47/88 (53%), Gaps = 1/88 (1%)
Frame = +3
Query: 36 EAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQL- 212
+ QE V D + ++K +E K K K++ V + T + +EE+ K+EK++ +
Sbjct: 218 KTQETEVTDDEAELDEDKKPEEEKP---KDDKVEDVTDEKVTDVTDEEEKKEEKKKKKRK 274
Query: 213 LTEITKEHELRPVTTEVKQRLPSQIDIE 296
+T +T+E E+ + RLPS++ E
Sbjct: 275 VTNVTREWEMLNKQKPIWMRLPSEVTNE 302
>sp|O67124|RAD50_AQUAE Probable DNA double-strand break repair rad50 ATPase
Length = 978
Score = 36.2 bits (82), Expect = 0.026
Identities = 26/94 (27%), Positives = 46/94 (48%), Gaps = 5/94 (5%)
Frame = +3
Query: 6 EFQKDKLKHVEAQEKVVLPDHDTIKQEKTEQELKDEIEKG-----TKLKHVETTEKTVLP 170
E + LK++E + K + + ++QE + E KD +E+ TKLK +E EK V
Sbjct: 203 EVLEKTLKNLEEELKELKETEEKLRQELKKAEEKDSLERELSQVVTKLKELENLEKEVEK 262
Query: 171 NEEDIKKEKQEAQLLTEITKEHELRPVTTEVKQR 272
E ++ ++ A + + E+ TE+K R
Sbjct: 263 LREKLEFSRKVAPYVPIAKRIEEIDKKLTELKVR 296
>sp|P21753|TYB9_PIG Thymosin beta-9
Length = 41
Score = 35.8 bits (81), Expect = 0.034
Identities = 17/29 (58%), Positives = 19/29 (65%)
Frame = +3
Query: 3 NEFQKDKLKHVEAQEKVVLPDHDTIKQEK 89
N F K KLK E QEK LP +TI+QEK
Sbjct: 10 NSFDKAKLKKTETQEKNTLPTKETIEQEK 38
Score = 31.2 bits (69), Expect = 0.84
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +3
Query: 129 KLKHVETTEKTVLPNEEDIKKEKQ 200
KLK ET EK LP +E I++EKQ
Sbjct: 16 KLKKTETQEKNTLPTKETIEQEKQ 39
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,394,470
Number of Sequences: 369166
Number of extensions: 589398
Number of successful extensions: 3713
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3251
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3614
length of database: 68,354,980
effective HSP length: 99
effective length of database: 50,066,215
effective search space used: 1852449955
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)