Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_F09
(381 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P02829|HSP82_YEAST ATP-dependent molecular chaperone HSP... 40 0.001
sp|P18281|ACTO_ACACA Actobindin 39 0.005
sp|P46821|MAP1B_HUMAN Microtubule-associated protein 1B (MA... 38 0.006
sp|P15108|HSC82_YEAST ATP-dependent molecular chaperone HSC... 38 0.006
sp|P15205|MAP1B_RAT Microtubule-associated protein 1B (MAP ... 37 0.011
sp|P14873|MAP1B_MOUSE Microtubule-associated protein 1B (MA... 37 0.014
sp|P24724|HSP90_THEPA Heat shock protein 90 (HSP90) 37 0.018
sp|Q25293|HSP83_LEIIN Heat shock protein 83-1 (HSP 83) 35 0.053
sp|O67720|YI73_AQUAE Hypothetical protein AQ_1873 35 0.053
sp|O08638|MYH11_MOUSE Myosin-11 (Myosin heavy chain, smooth... 35 0.069
>sp|P02829|HSP82_YEAST ATP-dependent molecular chaperone HSP82 (Heat shock protein Hsp90
heat inducible isoform) (82 kDa heat shock protein)
Length = 709
Score = 40.4 bits (93), Expect = 0.001
Identities = 22/69 (31%), Positives = 39/69 (56%)
Frame = +1
Query: 4 EAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLL 183
E +++V +P+ + +EK ++E KDE +K KL+ V+ E+ + KK K+E Q +
Sbjct: 212 EVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEEEE----KKPKTKKVKEEVQEI 267
Query: 184 TEITKEHEL 210
E+ K L
Sbjct: 268 EELNKTKPL 276
>sp|P18281|ACTO_ACACA Actobindin
Length = 88
Score = 38.5 bits (88), Expect = 0.005
Identities = 22/69 (31%), Positives = 32/69 (46%)
Frame = +1
Query: 67 ELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITKEHELRPVTTEVKQRLP 246
EL+ I +G LKH ET +K+ E K+ + L E+ K HEL+ T K
Sbjct: 4 ELQSAIGQGAALKHAETVDKSAPQIENVTVKKVDRSSFLEEVAKPHELKHAETVDKSGPA 63
Query: 247 SQIDIEAEK 273
D+ +K
Sbjct: 64 IPEDVHVKK 72
>sp|P46821|MAP1B_HUMAN Microtubule-associated protein 1B (MAP 1B) [Contains: MAP1 light
chain LC1]
Length = 2468
Score = 38.1 bits (87), Expect = 0.006
Identities = 24/79 (30%), Positives = 42/79 (53%), Gaps = 1/79 (1%)
Frame = +1
Query: 40 TIKQE-KTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITKEHELRP 216
T+K+E K + E K E ++ K + + +KT + EE KKE+ + ++ EI KE + P
Sbjct: 637 TVKKETKVKPEDKKEEKEKPKKEVAKKEDKTPIKKEEKPKKEEVKKEVKKEIKKEEKKEP 696
Query: 217 VTTEVKQRLPSQIDIEAEK 273
K+ P ++ E +K
Sbjct: 697 KKEVKKETPPKEVKKEVKK 715
Score = 35.8 bits (81), Expect = 0.031
Identities = 21/88 (23%), Positives = 42/88 (47%), Gaps = 6/88 (6%)
Frame = +1
Query: 28 PDHDTIKQEKTEQELKDEIEKGTKLKHV------ETTEKTVLPNEEDIKKEKQEAQLLTE 189
P + + +E K+E + TK+ HV E+ EK ++ ++ +K E + + E
Sbjct: 547 PAAKPLPSKSVRKESKEETPEVTKVNHVEKPPKVESKEKVMVKKDKPVKTETKPSVTEKE 606
Query: 190 ITKEHELRPVTTEVKQRLPSQIDIEAEK 273
+ + E PV EV ++ + + +A K
Sbjct: 607 VPSKEEPSPVKAEVAEKQATDVKPKAAK 634
Score = 32.7 bits (73), Expect = 0.26
Identities = 18/78 (23%), Positives = 43/78 (55%), Gaps = 2/78 (2%)
Frame = +1
Query: 10 QEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNE--EDIKKEKQEAQLL 183
++K + + K+E+ ++E+K EI+K K + + +K P E +++KKE+++
Sbjct: 664 EDKTPIKKEEKPKKEEVKKEVKKEIKKEEKKEPKKEVKKETPPKEVKKEVKKEEKKEVKK 723
Query: 184 TEITKEHELRPVTTEVKQ 237
E + E++ + + K+
Sbjct: 724 EEKEPKKEIKKLPKDAKK 741
>sp|P15108|HSC82_YEAST ATP-dependent molecular chaperone HSC82 (Heat shock protein Hsp90
constitutive isoform) (82 kDa heat shock cognate
protein)
Length = 705
Score = 38.1 bits (87), Expect = 0.006
Identities = 20/62 (32%), Positives = 37/62 (59%)
Frame = +1
Query: 4 EAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLL 183
E +++V +P+ +EK ++E KDE +K KL+ V+ E+ P + +K+E QE + L
Sbjct: 212 EVEKEVPIPE-----EEKKDEEKKDEDDKKPKLEEVDEEEEEKKPKTKKVKEEVQELEEL 266
Query: 184 TE 189
+
Sbjct: 267 NK 268
>sp|P15205|MAP1B_RAT Microtubule-associated protein 1B (MAP 1B) (Neuraxin) [Contains:
MAP1 light chain LC1]
Length = 2459
Score = 37.4 bits (85), Expect = 0.011
Identities = 26/79 (32%), Positives = 42/79 (53%)
Frame = +1
Query: 10 QEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTE 189
++KVV + T +EK E++ K E+ K +KT L +E KKE+ + ++ E
Sbjct: 633 KDKVVKKEIKTKPEEKKEEKPKKEVAK--------KEDKTPLKKDEKPKKEEAKKEIKKE 684
Query: 190 ITKEHELRPVTTEVKQRLP 246
I KE E + + EVK+ P
Sbjct: 685 IKKE-EKKELKKEVKKETP 702
Score = 31.6 bits (70), Expect = 0.59
Identities = 19/81 (23%), Positives = 36/81 (44%), Gaps = 6/81 (7%)
Frame = +1
Query: 28 PDHDTIKQEKTEQELKDEIEKGTKLKHVETT------EKTVLPNEEDIKKEKQEAQLLTE 189
P + + +E K+E + TK VE T EK ++ ++ K E + + E
Sbjct: 546 PATKPLSSKSVRKESKEEAPEATKASQVEKTPKVESKEKVIVKKDKPGKVESKPSVTEKE 605
Query: 190 ITKEHELRPVTTEVKQRLPSQ 252
+ + E PV EV ++ ++
Sbjct: 606 VPSKEEQSPVKAEVAEKAATE 626
>sp|P14873|MAP1B_MOUSE Microtubule-associated protein 1B (MAP 1B) (MAP1.2) (MAP1(X))
[Contains: MAP1 light chain LC1]
Length = 2464
Score = 37.0 bits (84), Expect = 0.014
Identities = 26/95 (27%), Positives = 48/95 (50%), Gaps = 7/95 (7%)
Frame = +1
Query: 10 QEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKE-------KQ 168
++KVV + T +EK E++ K E+ K ++ EK P +E++KKE ++
Sbjct: 634 KDKVVKKEIKTKLEEKKEEKPKKEVVKKEDKTPLKKDEK---PRKEEVKKEIKKEIKKEE 690
Query: 169 EAQLLTEITKEHELRPVTTEVKQRLPSQIDIEAEK 273
+L E+ KE L+ EVK+ ++ E ++
Sbjct: 691 RKELKKEVKKETPLKDAKKEVKKEEKKEVKKEEKE 725
Score = 34.7 bits (78), Expect = 0.069
Identities = 19/84 (22%), Positives = 47/84 (55%), Gaps = 2/84 (2%)
Frame = +1
Query: 1 VEAQEKVVLPDHDTIKQEKTEQELKDEIEKGTK--LKHVETTEKTVLPNEEDIKKEKQEA 174
V+ ++K L + ++E+ ++E+K EI+K + LK E + ++++KKE+++
Sbjct: 659 VKKEDKTPLKKDEKPRKEEVKKEIKKEIKKEERKELKKEVKKETPLKDAKKEVKKEEKKE 718
Query: 175 QLLTEITKEHELRPVTTEVKQRLP 246
E + E++ ++ ++K+ P
Sbjct: 719 VKKEEKEPKKEIKKISKDIKKSTP 742
Score = 33.1 bits (74), Expect = 0.20
Identities = 19/77 (24%), Positives = 36/77 (46%), Gaps = 6/77 (7%)
Frame = +1
Query: 28 PDHDTIKQEKTEQELKDEIEKGTKLKHVETT------EKTVLPNEEDIKKEKQEAQLLTE 189
P + + +E K+E + TK VE T EK ++ ++ +K E + + E
Sbjct: 547 PATKPVASKSVRKESKEETPEVTKTSQVEKTPKVESKEKVLVKKDKPVKTESKPSVTEKE 606
Query: 190 ITKEHELRPVTTEVKQR 240
++ + E PV EV ++
Sbjct: 607 VSSKEEQSPVKAEVAEK 623
>sp|P24724|HSP90_THEPA Heat shock protein 90 (HSP90)
Length = 721
Score = 36.6 bits (83), Expect = 0.018
Identities = 25/88 (28%), Positives = 47/88 (53%), Gaps = 1/88 (1%)
Frame = +1
Query: 4 EAQEKVVLPDHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQL- 180
+ QE V D + ++K +E K K K++ V + T + +EE+ K+EK++ +
Sbjct: 218 KTQETEVTDDEAELDEDKKPEEEKP---KDDKVEDVTDEKVTDVTDEEEKKEEKKKKKRK 274
Query: 181 LTEITKEHELRPVTTEVKQRLPSQIDIE 264
+T +T+E E+ + RLPS++ E
Sbjct: 275 VTNVTREWEMLNKQKPIWMRLPSEVTNE 302
>sp|Q25293|HSP83_LEIIN Heat shock protein 83-1 (HSP 83)
Length = 701
Score = 35.0 bits (79), Expect = 0.053
Identities = 24/78 (30%), Positives = 42/78 (53%)
Frame = +1
Query: 31 DHDTIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITKEHEL 210
D + + ++ TE+E+ DE E+ TK K E E V E + EK++ + + E+TKE+E+
Sbjct: 202 DIELMVEKTTEKEVTDEDEEDTK-KADEDEEPKVEEVREGDEGEKKKTKKVKEVTKEYEV 260
Query: 211 RPVTTEVKQRLPSQIDIE 264
+ + R P + E
Sbjct: 261 QNKHKPLWTRDPKDVTKE 278
>sp|O67720|YI73_AQUAE Hypothetical protein AQ_1873
Length = 407
Score = 35.0 bits (79), Expect = 0.053
Identities = 27/104 (25%), Positives = 49/104 (47%), Gaps = 17/104 (16%)
Frame = +1
Query: 13 EKVVLPDHDTIKQEKTEQELKDEIE------KGTKLKHVETTE------KTVLPNE---- 144
EK+ LP+ T+ ++ + + +E+E K ++K +ET E + +P E
Sbjct: 235 EKINLPNKSTLNRDAGAEAIAEEVEKVAQKVKKEEIKSMETEEVKEEKVREEIPEEVPQE 294
Query: 145 -EDIKKEKQEAQLLTEITKEHELRPVTTEVKQRLPSQIDIEAEK 273
E+IK+EK E + E T+ K++ P + + AEK
Sbjct: 295 VEEIKEEKPEMSEIKEKTE-------AISTKEKTPEAVQVLAEK 331
>sp|O08638|MYH11_MOUSE Myosin-11 (Myosin heavy chain, smooth muscle isoform) (SMMHC)
Length = 1972
Score = 34.7 bits (78), Expect = 0.069
Identities = 22/73 (30%), Positives = 39/73 (53%)
Frame = +1
Query: 40 TIKQEKTEQELKDEIEKGTKLKHVETTEKTVLPNEEDIKKEKQEAQLLTEITKEHELRPV 219
T +Q+K E ELK+ +K T+L +T + L E ++ E +E ++ K+ EL +
Sbjct: 865 TERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAESEEMRVRL-AAKKQELEEI 923
Query: 220 TTEVKQRLPSQID 258
E++ RL + D
Sbjct: 924 LHEMEARLEEEED 936
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,595,964
Number of Sequences: 369166
Number of extensions: 532714
Number of successful extensions: 3277
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2921
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3208
length of database: 68,354,980
effective HSP length: 93
effective length of database: 51,174,625
effective search space used: 1688762625
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)