Planaria EST Database

DrC_00045

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00045
         (1295 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8BM96|GP128_MOUSE  Probable G-protein coupled receptor 1...    38   0.056
sp|P10845|BXA1_CLOBO  Botulinum neurotoxin type A precursor ...    37   0.16 
sp|Q45894|BXA2_CLOBO  Botulinum neurotoxin type A precursor ...    34   0.80 
sp|Q07566|IPGD_SHIFL  Inositol phosphate phosphatase ipgD (E...    33   1.4  
sp|Q55286|IPGD_SHISO  Inositol phosphate phosphatase ipgD (E...    33   1.4  
sp|Q8T5T1|MDN1_GIALA  Midasin (MIDAS-containing protein)           33   1.8  
sp|Q12124|MED2_YEAST  RNA polymerase II mediator complex sub...    33   2.3  
sp|Q9RMG3|CR2AD_BACTU  Pesticidal crystal protein cry2Ad (In...    33   2.3  
sp|P54674|PI3K2_DICDI  Phosphatidylinositol 3-kinase 2 (PI3-...    32   5.2  
sp|P21254|CR2AB_BACTK  Pesticidal crystal protein cry2Ab (In...    32   5.2  

>sp|Q8BM96|GP128_MOUSE Probable G-protein coupled receptor 128 precursor
          Length = 785

 Score = 38.1 bits (87), Expect = 0.056
 Identities = 28/121 (23%), Positives = 57/121 (47%), Gaps = 4/121 (3%)
 Frame = +1

Query: 649 IEQLNRQMYRKGGRGV-NWGLLTSMGTNNFVLGPSTVQGQLSGNGNSIVSRGIISNVTSN 825
           I+Q+    Y +G + V    +     T +   GPS +     G+ NS+VS  I+ N T+N
Sbjct: 234 IKQMENYSYSQGDQTVVEPNIAIQSVTRDDNSGPSVLFSVQKGSSNSLVSGRILINKTAN 293

Query: 826 GASYNNRLPIEA---SGDSPEICGLQILSNDSESSELHENLDFNLKVDYKMELDDNKNNR 996
           G + + +  ++    +G++ + CG  +  N     +L ++  F    D+  ++  +K N 
Sbjct: 294 GFNPDGQTELQILLNTGENRKSCGFMVYQN----HKLFQSKTFTATSDFSQKIISSKINE 349

Query: 997 T 999
           +
Sbjct: 350 S 350

>sp|P10845|BXA1_CLOBO Botulinum neurotoxin type A precursor (BoNT/A) (Bontoxilysin A)
            (BOTOX) [Contains: Botulinum neurotoxin A light-chain;
            Botulinum neurotoxin A heavy-chain]
          Length = 1296

 Score = 36.6 bits (83), Expect = 0.16
 Identities = 37/144 (25%), Positives = 57/144 (39%), Gaps = 11/144 (7%)
 Frame = +1

Query: 670  MYRKGGRGVNWGLLTSMGTNNFVLGPSTVQGQ-------LSGNGNSIVSRG--IISNVTS 822
            MY KG RG       S+ T N  L  S  +G         SGN ++IV     +  NV  
Sbjct: 1134 MYLKGPRG-------SVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVV 1186

Query: 823  NGASYNNRLPIEASGDSPEICGLQI--LSNDSESSELHENLDFNLKVDYKMELDDNKNNR 996
                Y        +G    +  L+I  + N S+   +    D  +    KM L DN  N 
Sbjct: 1187 KNKEYRLATNASQAGVEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGND 1246

Query: 997  TSNSGITEKHHSPELVKNDWINQE 1068
                G  + ++  +LV ++W N++
Sbjct: 1247 IGFIGFHQFNNIAKLVASNWYNRQ 1270

>sp|Q45894|BXA2_CLOBO Botulinum neurotoxin type A precursor (BoNT/A) (Bontoxilysin A)
            (BOTOX) [Contains: Botulinum neurotoxin A light-chain;
            Botulinum neurotoxin A heavy-chain]
          Length = 1296

 Score = 34.3 bits (77), Expect = 0.80
 Identities = 36/144 (25%), Positives = 56/144 (38%), Gaps = 11/144 (7%)
 Frame = +1

Query: 670  MYRKGGRGVNWGLLTSMGTNNFVLGPSTVQGQ-------LSGNGNSIVSRG--IISNVTS 822
            MY KG RG       S+ T N  L  +  +G         SGN ++IV     +  NV  
Sbjct: 1134 MYLKGPRG-------SVVTTNIYLNSTLYEGTKFIIKKYASGNEDNIVRNNDRVYINVVV 1186

Query: 823  NGASYNNRLPIEASGDSPEICGLQI--LSNDSESSELHENLDFNLKVDYKMELDDNKNNR 996
                Y        +G    +  L+I  + N S+   +    D  ++   KM L DN  N 
Sbjct: 1187 KNKEYRLATNASQAGVEKILSALEIPDVGNLSQVVVMKSKDDQGIRNKCKMNLQDNNGND 1246

Query: 997  TSNSGITEKHHSPELVKNDWINQE 1068
                G     +  +LV ++W N++
Sbjct: 1247 IGFIGFHLYDNIAKLVASNWYNRQ 1270

>sp|Q07566|IPGD_SHIFL Inositol phosphate phosphatase ipgD (Effector protein ipgD)
          Length = 538

 Score = 33.5 bits (75), Expect = 1.4
 Identities = 19/60 (31%), Positives = 36/60 (60%), Gaps = 3/60 (5%)
 Frame = +1

Query: 73  ELMVNNNP--VSEVTIDFSAKESNIS-NELEVHCGSCKRLLDKQNNESYCNILKDHYLES 243
           +L++ N+   + EV+++      N   NEL +  G   R +DK N+ES C++L D++L++
Sbjct: 314 KLLIRNSDGLLKEVSVNLKVVTFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKN 373

>sp|Q55286|IPGD_SHISO Inositol phosphate phosphatase ipgD (Effector protein ipgD)
          Length = 538

 Score = 33.5 bits (75), Expect = 1.4
 Identities = 19/60 (31%), Positives = 36/60 (60%), Gaps = 3/60 (5%)
 Frame = +1

Query: 73  ELMVNNNP--VSEVTIDFSAKESNIS-NELEVHCGSCKRLLDKQNNESYCNILKDHYLES 243
           +L++ N+   + EV+++      N   NEL +  G   R +DK N+ES C++L D++L++
Sbjct: 314 KLLIRNSDGLLKEVSVNLKVVTFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKN 373

>sp|Q8T5T1|MDN1_GIALA Midasin (MIDAS-containing protein)
          Length = 4835

 Score = 33.1 bits (74), Expect = 1.8
 Identities = 17/47 (36%), Positives = 24/47 (51%)
 Frame = +1

Query: 889  LQILSNDSESSELHENLDFNLKVDYKMELDDNKNNRTSNSGITEKHH 1029
            L I   +SE  E  E  D  L+ +  +E DD+K+ R    G+TE  H
Sbjct: 4403 LNIHDRESEKDEAAEPQDMPLQSNKTVEFDDSKSGRDGALGLTESKH 4449

>sp|Q12124|MED2_YEAST RNA polymerase II mediator complex subunit 2
          Length = 431

 Score = 32.7 bits (73), Expect = 2.3
 Identities = 27/97 (27%), Positives = 40/97 (41%)
 Frame = +1

Query: 721  GTNNFVLGPSTVQGQLSGNGNSIVSRGIISNVTSNGASYNNRLPIEASGDSPEICGLQIL 900
            G N   +  S+++  ++ N NS          T NG   NN      +GD       +  
Sbjct: 267  GNNYNDINISSIENNINNNINS----------TKNGKDNNNESNKNNNGDEKNKNNNEDN 316

Query: 901  SNDSESSELHENLDFNLKVDYKMELDDNKNNRTSNSG 1011
             N++ SSE + N + N         DDN NN  +NSG
Sbjct: 317  ENNNNSSEKNNNNNNN----NNNNNDDNGNNNNNNSG 349

>sp|Q9RMG3|CR2AD_BACTU Pesticidal crystal protein cry2Ad (Insecticidal delta-endotoxin
           CryIIA(d)) (Crystaline entomocidal protoxin) (71 kDa
           crystal protein)
          Length = 633

 Score = 32.7 bits (73), Expect = 2.3
 Identities = 28/131 (21%), Positives = 54/131 (41%), Gaps = 3/131 (2%)
 Frame = +1

Query: 289 AALDPGQMYWCKTCILIFEKPWSLFQHMADKAKGSKVQRWEKKIHLDWLDTVAGLMAGY- 465
           + L+ G+   C    ++   P+S FQH +      +   W+K  H  ++D + G +A + 
Sbjct: 3   SVLNSGRNTICDAYNVVVHDPFS-FQHKSLDTIQKEWMEWKKDNHSLYVDPIVGTVASFL 61

Query: 466 --DLGLFSLGKLRNDLRNLLADQHTIXXXXXXXXXXXXXXXQWLNPATIGNPWRLQQREM 639
              LG     ++ ++LRNL+    +                Q LN  T+      +   +
Sbjct: 62  LKKLGSLIGKRILSELRNLIFPSGSTNLMEDILRETEKFLNQKLNTDTLSRV-NAELTGL 120

Query: 640 MRKIEQLNRQM 672
              +E+ NRQ+
Sbjct: 121 QANVEEFNRQV 131

>sp|P54674|PI3K2_DICDI Phosphatidylinositol 3-kinase 2 (PI3-kinase) (PtdIns-3-kinase) (PI3K)
          Length = 1858

 Score = 31.6 bits (70), Expect = 5.2
 Identities = 28/108 (25%), Positives = 45/108 (41%), Gaps = 14/108 (12%)
 Frame = +1

Query: 769  SGNGNSIVSRGIISNVTSNGASYNNRLPIEASGDSPEICGLQILSNDSESS--------- 921
            + N NS +SRG I +   NG+   N        + P + G+Q  S  + S          
Sbjct: 1051 NNNSNSNISRGSIDS-EGNGSGSGN------GSEQPTLIGVQNFSLPNNSKLPINIVKRL 1103

Query: 922  -----ELHENLDFNLKVDYKMELDDNKNNRTSNSGITEKHHSPELVKN 1050
                     NL+FN   D + +  D KNN+ +   + E ++  EL+ N
Sbjct: 1104 FRVNIAGLRNLNFNNNEDARNKFADGKNNQPNVFVMAELYYGGELLTN 1151

>sp|P21254|CR2AB_BACTK Pesticidal crystal protein cry2Ab (Insecticidal delta-endotoxin
           CryIIA(b)) (Crystaline entomocidal protoxin) (71 kDa
           crystal protein)
          Length = 633

 Score = 31.6 bits (70), Expect = 5.2
 Identities = 28/131 (21%), Positives = 53/131 (40%), Gaps = 3/131 (2%)
 Frame = +1

Query: 289 AALDPGQMYWCKTCILIFEKPWSLFQHMADKAKGSKVQRWEKKIHLDWLDTVAGLMAGY- 465
           + L+ G+   C    +    P+S FQH +      +   W+K  H  +LD + G +A + 
Sbjct: 3   SVLNSGRTTICDAYNVAAHDPFS-FQHKSLDTVQKEWTEWKKNNHSLYLDPIVGTVASFL 61

Query: 466 --DLGLFSLGKLRNDLRNLLADQHTIXXXXXXXXXXXXXXXQWLNPATIGNPWRLQQREM 639
              +G     ++ ++LRNL+    +                Q LN  T+      +   +
Sbjct: 62  LKKVGSLVGKRILSELRNLIFPSGSTNLMQDILRETEKFLNQRLNTDTLARV-NAELTGL 120

Query: 640 MRKIEQLNRQM 672
              +E+ NRQ+
Sbjct: 121 QANVEEFNRQV 131

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 132,762,232
Number of Sequences: 369166
Number of extensions: 2590830
Number of successful extensions: 7309
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6955
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 7301
length of database: 68,354,980
effective HSP length: 114
effective length of database: 47,295,190
effective search space used: 14992575230
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00045

  1. Dr_sW_009_N06
  2. Dr_sW_002_C02
  3. Dr_sW_006_D20
  4. Dr_sW_010_N09
  5. Dr_sW_002_I08
  6. Dr_sW_018_H02
  7. Dr_sW_009_J23
  8. Dr_sW_017_F20
  9. Dr_sW_024_A19