Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_J23
(747 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8BM96|GP128_MOUSE Probable G-protein coupled receptor 1... 38 0.025
sp|P10845|BXA1_CLOBO Botulinum neurotoxin type A precursor ... 37 0.073
sp|Q45894|BXA2_CLOBO Botulinum neurotoxin type A precursor ... 34 0.36
sp|Q8T5T1|MDN1_GIALA Midasin (MIDAS-containing protein) 33 0.81
sp|Q12124|MED2_YEAST RNA polymerase II mediator complex sub... 33 1.1
sp|P54674|PI3K2_DICDI Phosphatidylinositol 3-kinase 2 (PI3-... 32 2.4
sp|P34278|YKK1_CAEEL Hypothetical protein C02F5.1 in chromo... 32 2.4
sp|Q09604|VAB15_CAEEL Homeobox protein vab-15 31 3.1
sp|P14164|BAF1_YEAST Transcription factor BAF1 (ARS-binding... 31 4.0
sp|Q9WV80|SNX1_MOUSE Sorting nexin-1 31 4.0
>sp|Q8BM96|GP128_MOUSE Probable G-protein coupled receptor 128 precursor
Length = 785
Score = 38.1 bits (87), Expect = 0.025
Identities = 28/121 (23%), Positives = 57/121 (47%), Gaps = 4/121 (3%)
Frame = +2
Query: 101 IEQLNRQMYRKGGRGV-NWGLLTSMGTNNFVLGPSTVQGQLSGNGNSIVSRGIISNVTSN 277
I+Q+ Y +G + V + T + GPS + G+ NS+VS I+ N T+N
Sbjct: 234 IKQMENYSYSQGDQTVVEPNIAIQSVTRDDNSGPSVLFSVQKGSSNSLVSGRILINKTAN 293
Query: 278 GASYNNRLPIEA---SGDSPEICGLQILSNDSESSELHENLDFNLKVDYKMELDDNKNNR 448
G + + + ++ +G++ + CG + N +L ++ F D+ ++ +K N
Sbjct: 294 GFNPDGQTELQILLNTGENRKSCGFMVYQN----HKLFQSKTFTATSDFSQKIISSKINE 349
Query: 449 T 451
+
Sbjct: 350 S 350
>sp|P10845|BXA1_CLOBO Botulinum neurotoxin type A precursor (BoNT/A) (Bontoxilysin A)
(BOTOX) [Contains: Botulinum neurotoxin A light-chain;
Botulinum neurotoxin A heavy-chain]
Length = 1296
Score = 36.6 bits (83), Expect = 0.073
Identities = 37/144 (25%), Positives = 57/144 (39%), Gaps = 11/144 (7%)
Frame = +2
Query: 122 MYRKGGRGVNWGLLTSMGTNNFVLGPSTVQGQ-------LSGNGNSIVSRG--IISNVTS 274
MY KG RG S+ T N L S +G SGN ++IV + NV
Sbjct: 1134 MYLKGPRG-------SVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVV 1186
Query: 275 NGASYNNRLPIEASGDSPEICGLQI--LSNDSESSELHENLDFNLKVDYKMELDDNKNNR 448
Y +G + L+I + N S+ + D + KM L DN N
Sbjct: 1187 KNKEYRLATNASQAGVEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGND 1246
Query: 449 TSNSGITEKHHSPELVKNDWINQE 520
G + ++ +LV ++W N++
Sbjct: 1247 IGFIGFHQFNNIAKLVASNWYNRQ 1270
>sp|Q45894|BXA2_CLOBO Botulinum neurotoxin type A precursor (BoNT/A) (Bontoxilysin A)
(BOTOX) [Contains: Botulinum neurotoxin A light-chain;
Botulinum neurotoxin A heavy-chain]
Length = 1296
Score = 34.3 bits (77), Expect = 0.36
Identities = 36/144 (25%), Positives = 56/144 (38%), Gaps = 11/144 (7%)
Frame = +2
Query: 122 MYRKGGRGVNWGLLTSMGTNNFVLGPSTVQGQ-------LSGNGNSIVSRG--IISNVTS 274
MY KG RG S+ T N L + +G SGN ++IV + NV
Sbjct: 1134 MYLKGPRG-------SVVTTNIYLNSTLYEGTKFIIKKYASGNEDNIVRNNDRVYINVVV 1186
Query: 275 NGASYNNRLPIEASGDSPEICGLQI--LSNDSESSELHENLDFNLKVDYKMELDDNKNNR 448
Y +G + L+I + N S+ + D ++ KM L DN N
Sbjct: 1187 KNKEYRLATNASQAGVEKILSALEIPDVGNLSQVVVMKSKDDQGIRNKCKMNLQDNNGND 1246
Query: 449 TSNSGITEKHHSPELVKNDWINQE 520
G + +LV ++W N++
Sbjct: 1247 IGFIGFHLYDNIAKLVASNWYNRQ 1270
>sp|Q8T5T1|MDN1_GIALA Midasin (MIDAS-containing protein)
Length = 4835
Score = 33.1 bits (74), Expect = 0.81
Identities = 17/47 (36%), Positives = 24/47 (51%)
Frame = +2
Query: 341 LQILSNDSESSELHENLDFNLKVDYKMELDDNKNNRTSNSGITEKHH 481
L I +SE E E D L+ + +E DD+K+ R G+TE H
Sbjct: 4403 LNIHDRESEKDEAAEPQDMPLQSNKTVEFDDSKSGRDGALGLTESKH 4449
>sp|Q12124|MED2_YEAST RNA polymerase II mediator complex subunit 2
Length = 431
Score = 32.7 bits (73), Expect = 1.1
Identities = 27/97 (27%), Positives = 40/97 (41%)
Frame = +2
Query: 173 GTNNFVLGPSTVQGQLSGNGNSIVSRGIISNVTSNGASYNNRLPIEASGDSPEICGLQIL 352
G N + S+++ ++ N NS T NG NN +GD +
Sbjct: 267 GNNYNDINISSIENNINNNINS----------TKNGKDNNNESNKNNNGDEKNKNNNEDN 316
Query: 353 SNDSESSELHENLDFNLKVDYKMELDDNKNNRTSNSG 463
N++ SSE + N + N DDN NN +NSG
Sbjct: 317 ENNNNSSEKNNNNNNN----NNNNNDDNGNNNNNNSG 349
>sp|P54674|PI3K2_DICDI Phosphatidylinositol 3-kinase 2 (PI3-kinase) (PtdIns-3-kinase) (PI3K)
Length = 1858
Score = 31.6 bits (70), Expect = 2.4
Identities = 28/108 (25%), Positives = 45/108 (41%), Gaps = 14/108 (12%)
Frame = +2
Query: 221 SGNGNSIVSRGIISNVTSNGASYNNRLPIEASGDSPEICGLQILSNDSESS--------- 373
+ N NS +SRG I + NG+ N + P + G+Q S + S
Sbjct: 1051 NNNSNSNISRGSIDS-EGNGSGSGN------GSEQPTLIGVQNFSLPNNSKLPINIVKRL 1103
Query: 374 -----ELHENLDFNLKVDYKMELDDNKNNRTSNSGITEKHHSPELVKN 502
NL+FN D + + D KNN+ + + E ++ EL+ N
Sbjct: 1104 FRVNIAGLRNLNFNNNEDARNKFADGKNNQPNVFVMAELYYGGELLTN 1151
>sp|P34278|YKK1_CAEEL Hypothetical protein C02F5.1 in chromosome III
Length = 1010
Score = 31.6 bits (70), Expect = 2.4
Identities = 27/107 (25%), Positives = 44/107 (41%), Gaps = 10/107 (9%)
Frame = +2
Query: 257 ISNVTSNGASYNNRLPIE------ASGDSPEICGLQILSN----DSESSELHENLDFNLK 406
I V + A+ N +P++ A G E + +N + ++S+L EN N
Sbjct: 183 IPKVPKHNATMNRTIPMDLDESKAAGGQCDETMNVFNFTNLEAAEMDTSKLDENNTMNA- 241
Query: 407 VDYKMELDDNKNNRTSNSGITEKHHSPELVKNDWINQEHISDLKSPQ 547
+ + N N + ITE H E KND + D+ +PQ
Sbjct: 242 ----IRIPINSNVMPVDMDITEHHTLIEEKKNDTFGPSQLMDISAPQ 284
>sp|Q09604|VAB15_CAEEL Homeobox protein vab-15
Length = 225
Score = 31.2 bits (69), Expect = 3.1
Identities = 23/65 (35%), Positives = 30/65 (46%), Gaps = 9/65 (13%)
Frame = +2
Query: 281 ASYNNRLPIEASGDSPEIC---------GLQILSNDSESSELHENLDFNLKVDYKMELDD 433
A NRLPI +S SPE C LQ LS+ E S ++ + + K L
Sbjct: 67 AQTGNRLPIVSSDSSPESCASSPLSMQHSLQWLSSQREDSPTSDDAKIQIGLS-KCMLRK 125
Query: 434 NKNNR 448
+KNNR
Sbjct: 126 HKNNR 130
>sp|P14164|BAF1_YEAST Transcription factor BAF1 (ARS-binding factor 1) (Protein ABF1)
(Bidirectionally acting factor) (SFB-B) (DNA replication
enhancer-binding protein OBF1)
Length = 731
Score = 30.8 bits (68), Expect = 4.0
Identities = 23/82 (28%), Positives = 41/82 (50%), Gaps = 4/82 (4%)
Frame = +2
Query: 257 ISNVTSNGASYNNRLPIEASGDSPEICGLQILSND-SESSELHENLDFNLK---VDYKME 424
+S++T + +++LP E + L++LS+ E LH+N D ++ VD +E
Sbjct: 629 LSDITLEERNEDDKLPHEVAEQ------LRLLSSHLKEVENLHQNNDDDVDDVMVDVDVE 682
Query: 425 LDDNKNNRTSNSGITEKHHSPE 490
NKN N+ ++ HH E
Sbjct: 683 SQYNKNTNHHNNHHSQPHHDEE 704
>sp|Q9WV80|SNX1_MOUSE Sorting nexin-1
Length = 522
Score = 30.8 bits (68), Expect = 4.0
Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 5/76 (6%)
Frame = +2
Query: 350 LSNDSESSELHENLDF---NLKVDYKMEL--DDNKNNRTSNSGITEKHHSPELVKNDWIN 514
+ N S+ + +HE+ D +L D +EL D +NN+ + G T HSP+ N
Sbjct: 68 IKNGSKENGIHEDQDQEPQDLFADATVELSLDSTQNNQKTMPGKTLTSHSPQEATNSPKP 127
Query: 515 QEHISDLKSPQWDESF 562
Q +L+ + ++ F
Sbjct: 128 QPSYEELEEEEQEDQF 143
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,597,376
Number of Sequences: 369166
Number of extensions: 1462429
Number of successful extensions: 4095
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3959
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4087
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6776504000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)