Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_F20
(592 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8BM96|GP128_MOUSE Probable G-protein coupled receptor 1... 37 0.037
sp|Q8T5T1|MDN1_GIALA Midasin (MIDAS-containing protein) 33 0.53
sp|Q12124|MED2_YEAST RNA polymerase II mediator complex sub... 33 0.70
sp|P10845|BXA1_CLOBO Botulinum neurotoxin type A precursor ... 32 1.2
sp|P54674|PI3K2_DICDI Phosphatidylinositol 3-kinase 2 (PI3-... 32 1.6
sp|P34278|YKK1_CAEEL Hypothetical protein C02F5.1 in chromo... 32 1.6
sp|Q09604|VAB15_CAEEL Homeobox protein vab-15 31 2.0
sp|P14164|BAF1_YEAST Transcription factor BAF1 (ARS-binding... 31 2.6
sp|Q9WV80|SNX1_MOUSE Sorting nexin-1 31 2.6
sp|P07248|ADR1_YEAST Regulatory protein ADR1 31 2.6
>sp|Q8BM96|GP128_MOUSE Probable G-protein coupled receptor 128 precursor
Length = 785
Score = 37.0 bits (84), Expect = 0.037
Identities = 22/89 (24%), Positives = 45/89 (50%), Gaps = 3/89 (3%)
Frame = +3
Query: 39 GPSTVQGQLSGNGNSIVSRGIISNVTSNGASYNNRLPIEA---SGDSPEICGLQILSNDS 209
GPS + G+ NS+VS I+ N T+NG + + + ++ +G++ + CG + N
Sbjct: 266 GPSVLFSVQKGSSNSLVSGRILINKTANGFNPDGQTELQILLNTGENRKSCGFMVYQN-- 323
Query: 210 ESSELHENLDFNLKVDYKMELDDNKNNRT 296
+L ++ F D+ ++ +K N +
Sbjct: 324 --HKLFQSKTFTATSDFSQKIISSKINES 350
>sp|Q8T5T1|MDN1_GIALA Midasin (MIDAS-containing protein)
Length = 4835
Score = 33.1 bits (74), Expect = 0.53
Identities = 17/47 (36%), Positives = 24/47 (51%)
Frame = +3
Query: 186 LQILSNDSESSELHENLDFNLKVDYKMELDDNKNNRTSNSGITEKHH 326
L I +SE E E D L+ + +E DD+K+ R G+TE H
Sbjct: 4403 LNIHDRESEKDEAAEPQDMPLQSNKTVEFDDSKSGRDGALGLTESKH 4449
>sp|Q12124|MED2_YEAST RNA polymerase II mediator complex subunit 2
Length = 431
Score = 32.7 bits (73), Expect = 0.70
Identities = 27/97 (27%), Positives = 40/97 (41%)
Frame = +3
Query: 18 GTNNFVLGPSTVQGQLSGNGNSIVSRGIISNVTSNGASYNNRLPIEASGDSPEICGLQIL 197
G N + S+++ ++ N NS T NG NN +GD +
Sbjct: 267 GNNYNDINISSIENNINNNINS----------TKNGKDNNNESNKNNNGDEKNKNNNEDN 316
Query: 198 SNDSESSELHENLDFNLKVDYKMELDDNKNNRTSNSG 308
N++ SSE + N + N DDN NN +NSG
Sbjct: 317 ENNNNSSEKNNNNNNN----NNNNNDDNGNNNNNNSG 349
>sp|P10845|BXA1_CLOBO Botulinum neurotoxin type A precursor (BoNT/A) (Bontoxilysin A)
(BOTOX) [Contains: Botulinum neurotoxin A light-chain;
Botulinum neurotoxin A heavy-chain]
Length = 1296
Score = 32.0 bits (71), Expect = 1.2
Identities = 31/129 (24%), Positives = 51/129 (39%), Gaps = 11/129 (8%)
Frame = +3
Query: 12 SMGTNNFVLGPSTVQGQ-------LSGNGNSIVSRG--IISNVTSNGASYNNRLPIEASG 164
S+ T N L S +G SGN ++IV + NV Y +G
Sbjct: 1142 SVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVVKNKEYRLATNASQAG 1201
Query: 165 DSPEICGLQI--LSNDSESSELHENLDFNLKVDYKMELDDNKNNRTSNSGITEKHHSPEL 338
+ L+I + N S+ + D + KM L DN N G + ++ +L
Sbjct: 1202 VEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGNDIGFIGFHQFNNIAKL 1261
Query: 339 VKNDWINQE 365
V ++W N++
Sbjct: 1262 VASNWYNRQ 1270
>sp|P54674|PI3K2_DICDI Phosphatidylinositol 3-kinase 2 (PI3-kinase) (PtdIns-3-kinase) (PI3K)
Length = 1858
Score = 31.6 bits (70), Expect = 1.6
Identities = 28/108 (25%), Positives = 45/108 (41%), Gaps = 14/108 (12%)
Frame = +3
Query: 66 SGNGNSIVSRGIISNVTSNGASYNNRLPIEASGDSPEICGLQILSNDSESS--------- 218
+ N NS +SRG I + NG+ N + P + G+Q S + S
Sbjct: 1051 NNNSNSNISRGSIDS-EGNGSGSGN------GSEQPTLIGVQNFSLPNNSKLPINIVKRL 1103
Query: 219 -----ELHENLDFNLKVDYKMELDDNKNNRTSNSGITEKHHSPELVKN 347
NL+FN D + + D KNN+ + + E ++ EL+ N
Sbjct: 1104 FRVNIAGLRNLNFNNNEDARNKFADGKNNQPNVFVMAELYYGGELLTN 1151
>sp|P34278|YKK1_CAEEL Hypothetical protein C02F5.1 in chromosome III
Length = 1010
Score = 31.6 bits (70), Expect = 1.6
Identities = 27/107 (25%), Positives = 44/107 (41%), Gaps = 10/107 (9%)
Frame = +3
Query: 102 ISNVTSNGASYNNRLPIE------ASGDSPEICGLQILSN----DSESSELHENLDFNLK 251
I V + A+ N +P++ A G E + +N + ++S+L EN N
Sbjct: 183 IPKVPKHNATMNRTIPMDLDESKAAGGQCDETMNVFNFTNLEAAEMDTSKLDENNTMNA- 241
Query: 252 VDYKMELDDNKNNRTSNSGITEKHHSPELVKNDWINQEHISDLKSPQ 392
+ + N N + ITE H E KND + D+ +PQ
Sbjct: 242 ----IRIPINSNVMPVDMDITEHHTLIEEKKNDTFGPSQLMDISAPQ 284
>sp|Q09604|VAB15_CAEEL Homeobox protein vab-15
Length = 225
Score = 31.2 bits (69), Expect = 2.0
Identities = 23/65 (35%), Positives = 30/65 (46%), Gaps = 9/65 (13%)
Frame = +3
Query: 126 ASYNNRLPIEASGDSPEIC---------GLQILSNDSESSELHENLDFNLKVDYKMELDD 278
A NRLPI +S SPE C LQ LS+ E S ++ + + K L
Sbjct: 67 AQTGNRLPIVSSDSSPESCASSPLSMQHSLQWLSSQREDSPTSDDAKIQIGLS-KCMLRK 125
Query: 279 NKNNR 293
+KNNR
Sbjct: 126 HKNNR 130
>sp|P14164|BAF1_YEAST Transcription factor BAF1 (ARS-binding factor 1) (Protein ABF1)
(Bidirectionally acting factor) (SFB-B) (DNA replication
enhancer-binding protein OBF1)
Length = 731
Score = 30.8 bits (68), Expect = 2.6
Identities = 23/82 (28%), Positives = 41/82 (50%), Gaps = 4/82 (4%)
Frame = +3
Query: 102 ISNVTSNGASYNNRLPIEASGDSPEICGLQILSND-SESSELHENLDFNLK---VDYKME 269
+S++T + +++LP E + L++LS+ E LH+N D ++ VD +E
Sbjct: 629 LSDITLEERNEDDKLPHEVAEQ------LRLLSSHLKEVENLHQNNDDDVDDVMVDVDVE 682
Query: 270 LDDNKNNRTSNSGITEKHHSPE 335
NKN N+ ++ HH E
Sbjct: 683 SQYNKNTNHHNNHHSQPHHDEE 704
>sp|Q9WV80|SNX1_MOUSE Sorting nexin-1
Length = 522
Score = 30.8 bits (68), Expect = 2.6
Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 5/76 (6%)
Frame = +3
Query: 195 LSNDSESSELHENLDF---NLKVDYKMEL--DDNKNNRTSNSGITEKHHSPELVKNDWIN 359
+ N S+ + +HE+ D +L D +EL D +NN+ + G T HSP+ N
Sbjct: 68 IKNGSKENGIHEDQDQEPQDLFADATVELSLDSTQNNQKTMPGKTLTSHSPQEATNSPKP 127
Query: 360 QEHISDLKSPQWDESF 407
Q +L+ + ++ F
Sbjct: 128 QPSYEELEEEEQEDQF 143
>sp|P07248|ADR1_YEAST Regulatory protein ADR1
Length = 1323
Score = 30.8 bits (68), Expect = 2.6
Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 3/45 (6%)
Frame = +3
Query: 189 QILSNDSESSELHENLDFNLKVDYKMELDDNKN---NRTSNSGIT 314
+++ D ++ EL + D N+ +D + LD N N NR+ +SG T
Sbjct: 261 ELVPLDLKNPELDSSFDLNMNLDLNLNLDSNFNIALNRSDSSGST 305
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,896,130
Number of Sequences: 369166
Number of extensions: 1009720
Number of successful extensions: 2763
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2681
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2755
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4455160255
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)