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Planaria EST Database

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00942
         (704 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O35459|ECH1_MOUSE  Delta3,5-delta2,4-dienoyl-CoA isomeras...   243   3e-64
sp|Q62651|ECH1_RAT  Delta3,5-delta2,4-dienoyl-CoA isomerase,...   240   3e-63
sp|Q5RFG0|ECH1_PONPY  Delta3,5-delta2,4-dienoyl-CoA isomeras...   233   3e-61
sp|P52046|CRT_CLOAB  3-hydroxybutyryl-CoA dehydratase (Croto...   110   3e-24
sp|P76082|PAAF_ECOLI  Probable enoyl-CoA hydratase paaF            93   8e-19
sp|P34559|ECHM_CAEEL  Probable enoyl-CoA hydratase, mitochon...    89   1e-17
sp|Q8BH95|ECHM_MOUSE  Enoyl-CoA hydratase, mitochondrial pre...    86   1e-16
sp|Q50130|ECHA6_MYCLE  Probable enoyl-CoA hydratase echA6          86   1e-16
sp|Q58DM8|ECHM_BOVIN  Enoyl-CoA hydratase, mitochondrial pre...    85   2e-16
sp|P14604|ECHM_RAT  Enoyl-CoA hydratase, mitochondrial precu...    83   6e-16

>sp|O35459|ECH1_MOUSE Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
          Length = 327

 Score =  243 bits (621), Expect = 3e-64
 Identities = 123/226 (54%), Positives = 163/226 (72%), Gaps = 3/226 (1%)
 Frame = +3

Query: 3   RCVVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKP 182
           R VV+SGAGK+F++GIDL +M   L     +D +R A+ +R LI  YQ +FT +E C KP
Sbjct: 102 RAVVVSGAGKMFTSGIDLMDMASELMQPSGDDAARIAWYLRDLISKYQKTFTVIEKCPKP 161

Query: 183 VIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLA 362
           VIAA+H  C+GG VD++S  DIR  T+DA+FQIKEV +GLAADVGTLQR PKVIGN SL 
Sbjct: 162 VIAAIHGGCIGGGVDLVSACDIRYCTQDAFFQIKEVDMGLAADVGTLQRLPKVIGNQSLV 221

Query: 363 RELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 542
            EL ++ARK  A+EAL  G +S +F DK+A+LN +F LA +I++ SPVAVQ SK  L YS
Sbjct: 222 NELTFSARKMMADEALDSGLVSRVFQDKDAMLNAAFALAADISSKSPVAVQGSKINLIYS 281

Query: 543 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQ---FAKL 671
           RDHS +E L   A WN  MLQ++D++KS+ A+++K   +   F+KL
Sbjct: 282 RDHSVDESLDYMATWNMSMLQTQDIIKSVQAAMEKRDTKSITFSKL 327

>sp|Q62651|ECH1_RAT Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
          Length = 327

 Score =  240 bits (612), Expect = 3e-63
 Identities = 122/226 (53%), Positives = 163/226 (72%), Gaps = 3/226 (1%)
 Frame = +3

Query: 3   RCVVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKP 182
           R VV+SGAGK+F++GIDL +M   +     +D +R A+ +R LI  YQ +FT +E C KP
Sbjct: 102 RAVVVSGAGKMFTSGIDLMDMASDILQPPGDDVARIAWYLRDLISRYQKTFTVIEKCPKP 161

Query: 183 VIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLA 362
           VIAA+H  C+GG VD+IS  DIR  T+DA+FQ+KEV +GLAADVGTLQR PKVIGN SL 
Sbjct: 162 VIAAIHGGCIGGGVDLISACDIRYCTQDAFFQVKEVDVGLAADVGTLQRLPKVIGNRSLV 221

Query: 363 RELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 542
            EL +TARK  A+EAL  G +S +F DK+ +LN +F LA +I++ SPVAVQ SK  L YS
Sbjct: 222 NELTFTARKMMADEALDSGLVSRVFPDKDVMLNAAFALAADISSKSPVAVQGSKINLIYS 281

Query: 543 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQ---FAKL 671
           RDHS +E L   A WN  MLQ++D++KS+ A+++K+  +   F+KL
Sbjct: 282 RDHSVDESLDYMATWNMSMLQTQDIIKSVQAAMEKKDSKSITFSKL 327

>sp|Q5RFG0|ECH1_PONPY Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
          Length = 328

 Score =  233 bits (595), Expect = 3e-61
 Identities = 115/217 (52%), Positives = 155/217 (71%)
 Frame = +3

Query: 3   RCVVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKP 182
           R VV+SGAGK+F+AG+DL +M   +   K +D +R ++ +R +I  YQ +F  +E C KP
Sbjct: 103 RAVVISGAGKMFTAGVDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIEKCPKP 162

Query: 183 VIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLA 362
           VIAAVH  C+GG VD+++  DIR   +DA+FQ+KEV +GLAADVGTLQR PKVIGN SL 
Sbjct: 163 VIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLV 222

Query: 363 RELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 542
            EL +TARK  A+EAL  G +S +F DKE +L+ +  LA EI++ SPVAVQ++K  L YS
Sbjct: 223 NELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYS 282

Query: 543 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEK 653
           RDHS  E L   A WN  MLQ++DLMKS+ A+ + ++
Sbjct: 283 RDHSVAESLNYVASWNMSMLQTQDLMKSVQAATENKE 319

>sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Crotonase)
          Length = 261

 Score =  110 bits (276), Expect = 3e-24
 Identities = 74/229 (32%), Positives = 126/229 (55%), Gaps = 1/229 (0%)
 Frame = +3

Query: 9   VVLSGAG-KVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPV 185
           V+L+GAG K F AG D+SEM ++              + R+  +     F  LEL  KPV
Sbjct: 52  VILTGAGEKSFVAGADISEMKEM-----------NTIEGRKFGILGNKVFRRLELLEKPV 100

Query: 186 IAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAR 365
           IAAV+   +GG  ++    DIR+++ +A F   EVGLG+    G  QR  +++G   +A+
Sbjct: 101 IAAVNGFALGGGCEIAMSCDIRIASSNARFGQPEVGLGITPGFGGTQRLSRLVGM-GMAK 159

Query: 366 ELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSR 545
           +L++TA+   A+EAL+IG ++ + +  E L+N + ++A +I + +PVAV+ SK A+N   
Sbjct: 160 QLIFTAQNIKADEALRIGLVNKVVEPSE-LMNTAKEIANKIVSNAPVAVKLSKQAINRGM 218

Query: 546 DHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQFAKL*FGYKNK 692
               +  L  +++       +ED   ++ A I+K K +      G+KN+
Sbjct: 219 QCDIDTALAFESEAFGECFSTEDQKDAMTAFIEKRKIE------GFKNR 261

>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
          Length = 255

 Score = 92.8 bits (229), Expect = 8e-19
 Identities = 62/217 (28%), Positives = 113/217 (52%)
 Frame = +3

Query: 12  VLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPVIA 191
           V++G  + F+AG DL+EM +       +D +      R  +     +F       KP+IA
Sbjct: 51  VITGNARFFAAGADLNEMAE-------KDLAATLNDTRPQLWARLQAFN------KPLIA 97

Query: 192 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 371
           AV+   +G   ++    D+ ++ ++A F + E+ LG+    G  QR  + +G  SLA ++
Sbjct: 98  AVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGK-SLASKM 156

Query: 372 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 551
           V +    TA++A + G +S +F   +  L  + +LA ++A  SP+A+QA+K AL  S++ 
Sbjct: 157 VLSGESITAQQAQQAGLVSDVFPS-DLTLEYALQLASKMARHSPLALQAAKQALRQSQEV 215

Query: 552 STEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
           + + GL  +     ++  +ED  + I A +QK  P F
Sbjct: 216 ALQAGLAQERQLFTLLAATEDRHEGISAFLQKRTPDF 252

>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
          Length = 288

 Score = 88.6 bits (218), Expect = 1e-17
 Identities = 62/218 (28%), Positives = 111/218 (50%)
 Frame = +3

Query: 9   VVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPVI 188
           +V++G+ + F+AG D+ EM        + +     F       ++ S++T++    KPVI
Sbjct: 83  IVITGSERAFAAGADIKEM--------TNNEFATTFS-----GSFLSNWTAVSDVKKPVI 129

Query: 189 AAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLARE 368
           AAV+   +GG  ++    DI  + + A F   E+ +G     G  QR+ +  G  S A E
Sbjct: 130 AAVNGFALGGGNELAMMCDIIYAGEKARFGQPEINIGTIPGAGGTQRWARAAGK-SFAME 188

Query: 369 LVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRD 548
           +  T    TA+EA + G +S IF   + ++ ++ KL  +IA  SP+ VQ +K A+N + +
Sbjct: 189 VCLTGNHVTAQEAKEHGIVSKIFP-ADQVVGEAVKLGEKIADQSPLIVQMAKEAVNKAYE 247

Query: 549 HSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
            + +EGL  +         ++D  + + A  +K KPQ+
Sbjct: 248 LTLQEGLHFERRLFHATFATKDRKEGMTAFAEKRKPQW 285

>sp|Q8BH95|ECHM_MOUSE Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score = 85.5 bits (210), Expect = 1e-16
 Identities = 65/222 (29%), Positives = 108/222 (48%), Gaps = 4/222 (1%)
 Frame = +3

Query: 9   VVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTS----LELCL 176
           +VL+G  K F+AG D+ EM              R FQ       Y S F S    +    
Sbjct: 85  IVLTGGDKAFAAGADIKEM------------QNRTFQDC-----YSSKFLSHWDHITRVK 127

Query: 177 KPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDS 356
           KPVIAAV+   +GG  ++    DI  + + A F   E+ LG     G  QR  + +G  S
Sbjct: 128 KPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGK-S 186

Query: 357 LARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALN 536
           LA E+V T  + +A++A + G +S IF   E L+ ++ + A +IA+ S + V  +K ++N
Sbjct: 187 LAMEMVLTGDRISAQDAKQAGLVSKIF-PVEKLVEEAIQCAEKIASNSKIVVAMAKESVN 245

Query: 537 YSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
            + + +  EG +++         ++D  + + A ++K K  F
Sbjct: 246 AAFEMTLTEGNKLEKRLFYSTFATDDRREGMTAFVEKRKANF 287

>sp|Q50130|ECHA6_MYCLE Probable enoyl-CoA hydratase echA6
          Length = 247

 Score = 85.5 bits (210), Expect = 1e-16
 Identities = 64/220 (29%), Positives = 106/220 (48%)
 Frame = +3

Query: 3   RCVVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKP 182
           R +VL+G G VF AG DLS      G   + D   R  ++ R+          ++    P
Sbjct: 49  RVIVLTGQGTVFCAGADLS------GDAFAADYPDRLIELHRV----------MDAVPMP 92

Query: 183 VIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLA 362
           VI A++   +G  + +    D+R+   DA+FQ      GLA D  +++R   ++G+   A
Sbjct: 93  VIGAINGPAIGAGLQLAMQCDLRVVAPDAYFQFPTSKYGLALDNWSIRRLSSLVGH-GRA 151

Query: 363 RELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 542
           R ++ TA K TA+ AL+ G  + I       L  +   A E+  L+P+A+Q +K  LN  
Sbjct: 152 RAMLLTAEKLTADIALQTGMANRI-----GALADAQAWAAEVTGLAPLAIQHAKRVLN-- 204

Query: 543 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
            D S EE              S+D++++ +A ++K  P+F
Sbjct: 205 DDGSIEEAWPEHKKLFDKAWTSQDVIEAQVARVEKRPPKF 244

>sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score = 85.1 bits (209), Expect = 2e-16
 Identities = 61/218 (27%), Positives = 108/218 (49%)
 Frame = +3

Query: 9   VVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPVI 188
           +VL+G  KVF+AG D+ EM  +             FQ       + S +  L    KPVI
Sbjct: 85  IVLTGGEKVFAAGADIKEMQSL------------TFQ-NCYSGGFLSHWDQLTRVKKPVI 131

Query: 189 AAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLARE 368
           AAV+   +GG  ++    DI  + + A F   E+ +G     G  QR  + +G  SLA E
Sbjct: 132 AAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILIGTIPGAGGTQRLTRAVGK-SLAME 190

Query: 369 LVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRD 548
           +V T  + +A++A + G +S IF   E ++ ++ + A +IA+ S +    +K ++N + +
Sbjct: 191 MVLTGDRISAQDAKQAGLVSKIF-PVETVVEEAIQCAEKIASNSKIVTAMAKESVNAAFE 249

Query: 549 HSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
            +  EG++++         +ED  + + A ++K K  F
Sbjct: 250 MTLAEGVKLEKKLFYSTFATEDRKEGMAAFVEKRKANF 287

>sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
           hydratase) (SCEH) (Enoyl-CoA hydratase 1)
          Length = 290

 Score = 83.2 bits (204), Expect = 6e-16
 Identities = 62/218 (28%), Positives = 106/218 (48%)
 Frame = +3

Query: 9   VVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPVI 188
           +VL+G  K F+AG D+ EM              R FQ       + S +  +    KPVI
Sbjct: 85  IVLTGGEKAFAAGADIKEM------------QNRTFQ-DCYSGKFLSHWDHITRIKKPVI 131

Query: 189 AAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLARE 368
           AAV+   +GG  ++    DI  + + A F   E+ LG     G  QR  + +G  SLA E
Sbjct: 132 AAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGK-SLAME 190

Query: 369 LVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRD 548
           +V T  + +A++A + G +S IF   E L+ ++ + A +IA  S + V  +K ++N + +
Sbjct: 191 MVLTGDRISAQDAKQAGLVSKIF-PVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFE 249

Query: 549 HSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
            +  EG +++         ++D  + + A ++K K  F
Sbjct: 250 MTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKANF 287

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,713,890
Number of Sequences: 369166
Number of extensions: 1793173
Number of successful extensions: 4952
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4907
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6170718545
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

1. Dr_sW_023_N18
2. Dr_sW_009_K01
3. Dr_sW_025_L13
4. Dr_sW_006_H14