Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_H14
(312 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O35459|ECH1_MOUSE Delta3,5-delta2,4-dienoyl-CoA isomeras... 92 3e-19
sp|Q62651|ECH1_RAT Delta3,5-delta2,4-dienoyl-CoA isomerase,... 91 8e-19
sp|Q5RFG0|ECH1_PONPY Delta3,5-delta2,4-dienoyl-CoA isomeras... 87 1e-17
sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF 51 9e-07
sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochon... 46 2e-05
sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Croto... 46 3e-05
sp|Q52995|ECHH_RHIME Probable enoyl-CoA hydratase 41 0.001
sp|Q8GB17|CAID_PROSL Carnitinyl-CoA dehydratase (Crotonobet... 38 0.006
sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8 >g... 37 0.011
sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitoch... 37 0.018
>sp|O35459|ECH1_MOUSE Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 327
Score = 92.4 bits (228), Expect = 3e-19
Identities = 47/95 (49%), Positives = 66/95 (69%), Gaps = 3/95 (3%)
Frame = +1
Query: 4 AGEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRM 183
A EAL G +S +F DK+A+LN +F LA +I++ SPVAVQ SK L YSRDHS +E L
Sbjct: 233 ADEALDSGLVSRVFQDKDAMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDESLDY 292
Query: 184 QADWNQVMLQSEDLMKSIMASIQKEKPQ---FAKL 279
A WN MLQ++D++KS+ A+++K + F+KL
Sbjct: 293 MATWNMSMLQTQDIIKSVQAAMEKRDTKSITFSKL 327
>sp|Q62651|ECH1_RAT Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 327
Score = 90.9 bits (224), Expect = 8e-19
Identities = 46/95 (48%), Positives = 66/95 (69%), Gaps = 3/95 (3%)
Frame = +1
Query: 4 AGEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRM 183
A EAL G +S +F DK+ +LN +F LA +I++ SPVAVQ SK L YSRDHS +E L
Sbjct: 233 ADEALDSGLVSRVFPDKDVMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDESLDY 292
Query: 184 QADWNQVMLQSEDLMKSIMASIQKEKPQ---FAKL 279
A WN MLQ++D++KS+ A+++K+ + F+KL
Sbjct: 293 MATWNMSMLQTQDIIKSVQAAMEKKDSKSITFSKL 327
>sp|Q5RFG0|ECH1_PONPY Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 328
Score = 87.0 bits (214), Expect = 1e-17
Identities = 43/86 (50%), Positives = 59/86 (68%)
Frame = +1
Query: 4 AGEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRM 183
A EAL G +S +F DKE +L+ + LA EI++ SPVAVQ++K L YSRDHS E L
Sbjct: 234 ADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNY 293
Query: 184 QADWNQVMLQSEDLMKSIMASIQKEK 261
A WN MLQ++DLMKS+ A+ + ++
Sbjct: 294 VASWNMSMLQTQDLMKSVQAATENKE 319
>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
Length = 255
Score = 50.8 bits (120), Expect = 9e-07
Identities = 29/90 (32%), Positives = 51/90 (56%)
Frame = +1
Query: 1 TAGEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLR 180
TA +A + G +S +F + L + +LA ++A SP+A+QA+K AL S++ + + GL
Sbjct: 164 TAQQAQQAGLVSDVFPS-DLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQAGLA 222
Query: 181 MQADWNQVMLQSEDLMKSIMASIQKEKPQF 270
+ ++ +ED + I A +QK P F
Sbjct: 223 QERQLFTLLAATEDRHEGISAFLQKRTPDF 252
>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
Length = 288
Score = 46.2 bits (108), Expect = 2e-05
Identities = 28/90 (31%), Positives = 50/90 (55%)
Frame = +1
Query: 1 TAGEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLR 180
TA EA + G +S IF + ++ ++ KL +IA SP+ VQ +K A+N + + + +EGL
Sbjct: 197 TAQEAKEHGIVSKIFP-ADQVVGEAVKLGEKIADQSPLIVQMAKEAVNKAYELTLQEGLH 255
Query: 181 MQADWNQVMLQSEDLMKSIMASIQKEKPQF 270
+ ++D + + A +K KPQ+
Sbjct: 256 FERRLFHATFATKDRKEGMTAFAEKRKPQW 285
>sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Crotonase)
Length = 261
Score = 45.8 bits (107), Expect = 3e-05
Identities = 29/99 (29%), Positives = 54/99 (54%)
Frame = +1
Query: 4 AGEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRM 183
A EAL+IG ++ + + E L+N + ++A +I + +PVAV+ SK A+N + L
Sbjct: 170 ADEALRIGLVNKVVEPSE-LMNTAKEIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAF 228
Query: 184 QADWNQVMLQSEDLMKSIMASIQKEKPQFAKL*FGYKNK 300
+++ +ED ++ A I+K K + G+KN+
Sbjct: 229 ESEAFGECFSTEDQKDAMTAFIEKRKIE------GFKNR 261
>sp|Q52995|ECHH_RHIME Probable enoyl-CoA hydratase
Length = 257
Score = 40.8 bits (94), Expect = 0.001
Identities = 27/89 (30%), Positives = 46/89 (51%)
Frame = +1
Query: 4 AGEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRM 183
A EA + G +S + + LL ++ A +IA+ S A +K A+N S + + EGLR
Sbjct: 167 AAEAERSGLVSRVVAP-DRLLEEALGAAEKIASFSLPAAMMAKEAVNRSLELTLAEGLRF 225
Query: 184 QADWNQVMLQSEDLMKSIMASIQKEKPQF 270
+ Q + +ED + + A + K K +F
Sbjct: 226 ERRLFQSLFATEDQKEGMAAFVAKRKAEF 254
>sp|Q8GB17|CAID_PROSL Carnitinyl-CoA dehydratase (Crotonobetainyl-CoA hydratase)
Length = 261
Score = 38.1 bits (87), Expect = 0.006
Identities = 27/93 (29%), Positives = 49/93 (52%), Gaps = 4/93 (4%)
Frame = +1
Query: 4 AGEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRM 183
A EAL+ G + + E L++ + +LA +IA +P+AV A K + + S EEG ++
Sbjct: 167 AQEALRWGIANRVVSATE-LMDSARELADQIANSAPLAVAALKEIYRATSELSIEEGYKL 225
Query: 184 QAD----WNQVMLQSEDLMKSIMASIQKEKPQF 270
+ +L SED ++ +A +K P++
Sbjct: 226 MRSGVLKYYPRVLHSEDALEGPLAFAEKRSPEW 258
>sp|P64017|ECHA8_MYCBO Probable enoyl-CoA hydratase echA8
sp|P64016|ECHA8_MYCTU Probable enoyl-CoA hydratase echA8
Length = 257
Score = 37.4 bits (85), Expect = 0.011
Identities = 26/89 (29%), Positives = 44/89 (49%)
Frame = +1
Query: 4 AGEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRM 183
A EA + G +S + + LL ++ A I+ +S A + +K A+N + + S EGL
Sbjct: 167 AAEAERSGLVSRVVPADD-LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLY 225
Query: 184 QADWNQVMLQSEDLMKSIMASIQKEKPQF 270
+ +ED + + A I+K PQF
Sbjct: 226 ERRLFHSAFATEDQSEGMAAFIEKRAPQF 254
>sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitochondrial precursor
(AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding enoyl-CoA hydratase) (muAUH)
Length = 314
Score = 36.6 bits (83), Expect = 0.018
Identities = 21/90 (23%), Positives = 44/90 (48%), Gaps = 3/90 (3%)
Frame = +1
Query: 10 EALKIGFISGIFDDKE---ALLNQSFKLAGEIAALSPVAVQASKAALNYSRDHSTEEGLR 180
EA +G IS + + + A ++ LA E PVA++ +K A+N + GL
Sbjct: 222 EAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLA 281
Query: 181 MQADWNQVMLQSEDLMKSIMASIQKEKPQF 270
++ + ++D ++ ++A +K P++
Sbjct: 282 IEEACYAQTISTKDRLEGLLAFKEKRPPRY 311
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,838,606
Number of Sequences: 369166
Number of extensions: 633278
Number of successful extensions: 1654
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1642
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1654
length of database: 68,354,980
effective HSP length: 72
effective length of database: 55,054,060
effective search space used: 1706675860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)