Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_K01
(598 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O35459|ECH1_MOUSE Delta3,5-delta2,4-dienoyl-CoA isomeras... 213 4e-55
sp|Q62651|ECH1_RAT Delta3,5-delta2,4-dienoyl-CoA isomerase,... 211 9e-55
sp|Q5RFG0|ECH1_PONPY Delta3,5-delta2,4-dienoyl-CoA isomeras... 201 9e-52
sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Croto... 100 3e-21
sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF 86 5e-17
sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochon... 82 8e-16
sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitoch... 79 9e-15
sp|Q8GB17|CAID_PROSL Carnitinyl-CoA dehydratase (Crotonobet... 79 1e-14
sp|Q13825|AUMH_HUMAN Methylglutaconyl-CoA hydratase, mitoch... 78 1e-14
sp|Q8Z9L5|CAID_SALTI Carnitinyl-CoA dehydratase (Crotonobet... 77 4e-14
>sp|O35459|ECH1_MOUSE Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 327
Score = 213 bits (541), Expect = 4e-55
Identities = 106/190 (55%), Positives = 139/190 (73%), Gaps = 3/190 (1%)
Frame = +2
Query: 5 AFQIRRLIMNYQSSFTSLELCLKPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEV 184
A+ +R LI YQ +FT +E C KPVIAA+H C+GG VD++S DIR T+DA+FQIKEV
Sbjct: 138 AWYLRDLISKYQKTFTVIEKCPKPVIAAIHGGCIGGGVDLVSACDIRYCTQDAFFQIKEV 197
Query: 185 GLGLAADVGTLQRFPKVIGNDSLARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSF 364
+GLAADVGTLQR PKVIGN SL EL ++ARK A+EAL G +S +F DK+A+LN +F
Sbjct: 198 DMGLAADVGTLQRLPKVIGNQSLVNELTFSARKMMADEALDSGLVSRVFQDKDAMLNAAF 257
Query: 365 KLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKE 544
LA +I++ SPVAVQ SK L YSRDHS +E L A WN MLQ++D++KS+ A+++K
Sbjct: 258 ALAADISSKSPVAVQGSKINLIYSRDHSVDESLDYMATWNMSMLQTQDIIKSVQAAMEKR 317
Query: 545 KPQ---FAKL 565
+ F+KL
Sbjct: 318 DTKSITFSKL 327
>sp|Q62651|ECH1_RAT Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 327
Score = 211 bits (538), Expect = 9e-55
Identities = 106/190 (55%), Positives = 139/190 (73%), Gaps = 3/190 (1%)
Frame = +2
Query: 5 AFQIRRLIMNYQSSFTSLELCLKPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEV 184
A+ +R LI YQ +FT +E C KPVIAA+H C+GG VD+IS DIR T+DA+FQ+KEV
Sbjct: 138 AWYLRDLISRYQKTFTVIEKCPKPVIAAIHGGCIGGGVDLISACDIRYCTQDAFFQVKEV 197
Query: 185 GLGLAADVGTLQRFPKVIGNDSLARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSF 364
+GLAADVGTLQR PKVIGN SL EL +TARK A+EAL G +S +F DK+ +LN +F
Sbjct: 198 DVGLAADVGTLQRLPKVIGNRSLVNELTFTARKMMADEALDSGLVSRVFPDKDVMLNAAF 257
Query: 365 KLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKE 544
LA +I++ SPVAVQ SK L YSRDHS +E L A WN MLQ++D++KS+ A+++K+
Sbjct: 258 ALAADISSKSPVAVQGSKINLIYSRDHSVDESLDYMATWNMSMLQTQDIIKSVQAAMEKK 317
Query: 545 KPQ---FAKL 565
+ F+KL
Sbjct: 318 DSKSITFSKL 327
>sp|Q5RFG0|ECH1_PONPY Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 328
Score = 201 bits (512), Expect = 9e-52
Identities = 98/181 (54%), Positives = 130/181 (71%)
Frame = +2
Query: 5 AFQIRRLIMNYQSSFTSLELCLKPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEV 184
++ +R +I YQ +F +E C KPVIAAVH C+GG VD+++ DIR +DA+FQ+KEV
Sbjct: 139 SWYLRDIITRYQETFNVIEKCPKPVIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEV 198
Query: 185 GLGLAADVGTLQRFPKVIGNDSLARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSF 364
+GLAADVGTLQR PKVIGN SL EL +TARK A+EAL G +S +F DKE +L+ +
Sbjct: 199 DVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRVFPDKEVMLDAAL 258
Query: 365 KLAGEIAALSPVAVQASKAALNYSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKE 544
LA EI++ SPVAVQ++K L YSRDHS E L A WN MLQ++DLMKS+ A+ + +
Sbjct: 259 ALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQDLMKSVQAATENK 318
Query: 545 K 547
+
Sbjct: 319 E 319
>sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Crotonase)
Length = 261
Score = 100 bits (249), Expect = 3e-21
Identities = 60/180 (33%), Positives = 104/180 (57%)
Frame = +2
Query: 47 FTSLELCLKPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRF 226
F LEL KPVIAAV+ +GG ++ DIR+++ +A F EVGLG+ G QR
Sbjct: 90 FRRLELLEKPVIAAVNGFALGGGCEIAMSCDIRIASSNARFGQPEVGLGITPGFGGTQRL 149
Query: 227 PKVIGNDSLARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAV 406
+++G +A++L++TA+ A+EAL+IG ++ + + E L+N + ++A +I + +PVAV
Sbjct: 150 SRLVGM-GMAKQLIFTAQNIKADEALRIGLVNKVVEPSE-LMNTAKEIANKIVSNAPVAV 207
Query: 407 QASKAALNYSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQFAKL*FGYKNK 586
+ SK A+N + L +++ +ED ++ A I+K K + G+KN+
Sbjct: 208 KLSKQAINRGMQCDIDTALAFESEAFGECFSTEDQKDAMTAFIEKRKIE------GFKNR 261
>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
Length = 255
Score = 86.3 bits (212), Expect = 5e-17
Identities = 50/162 (30%), Positives = 91/162 (56%)
Frame = +2
Query: 71 KPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDS 250
KP+IAAV+ +G ++ D+ ++ ++A F + E+ LG+ G QR + +G S
Sbjct: 93 KPLIAAVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGK-S 151
Query: 251 LARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALN 430
LA ++V + TA++A + G +S +F + L + +LA ++A SP+A+QA+K AL
Sbjct: 152 LASKMVLSGESITAQQAQQAGLVSDVFPS-DLTLEYALQLASKMARHSPLALQAAKQALR 210
Query: 431 YSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 556
S++ + + GL + ++ +ED + I A +QK P F
Sbjct: 211 QSQEVALQAGLAQERQLFTLLAATEDRHEGISAFLQKRTPDF 252
>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
Length = 288
Score = 82.4 bits (202), Expect = 8e-16
Identities = 53/175 (30%), Positives = 93/175 (53%)
Frame = +2
Query: 32 NYQSSFTSLELCLKPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVG 211
++ S++T++ KPVIAAV+ +GG ++ DI + + A F E+ +G G
Sbjct: 113 SFLSNWTAVSDVKKPVIAAVNGFALGGGNELAMMCDIIYAGEKARFGQPEINIGTIPGAG 172
Query: 212 TLQRFPKVIGNDSLARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAAL 391
QR+ + G S A E+ T TA+EA + G +S IF + ++ ++ KL +IA
Sbjct: 173 GTQRWARAAGK-SFAMEVCLTGNHVTAQEAKEHGIVSKIFP-ADQVVGEAVKLGEKIADQ 230
Query: 392 SPVAVQASKAALNYSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 556
SP+ VQ +K A+N + + + +EGL + ++D + + A +K KPQ+
Sbjct: 231 SPLIVQMAKEAVNKAYELTLQEGLHFERRLFHATFATKDRKEGMTAFAEKRKPQW 285
>sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitochondrial precursor
(AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding enoyl-CoA hydratase) (muAUH)
Length = 314
Score = 79.0 bits (193), Expect = 9e-15
Identities = 46/164 (28%), Positives = 85/164 (51%), Gaps = 3/164 (1%)
Frame = +2
Query: 74 PVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSL 253
P IAA+ +GG +++ DIR++ A + E L + G QR P+ IG SL
Sbjct: 149 PTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGM-SL 207
Query: 254 ARELVYTARKFTAEEALKIGFISGIFD---DKEALLNQSFKLAGEIAALSPVAVQASKAA 424
A+EL+++AR +EA +G IS + + + +A ++ LA E PVA++ +K A
Sbjct: 208 AKELIFSARVLDGQEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLA 267
Query: 425 LNYSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 556
+N + GL ++ + ++D ++ ++A +K P++
Sbjct: 268 INQGMEVDLVTGLAIEEACYAQTISTKDRLEGLLAFKEKRPPRY 311
>sp|Q8GB17|CAID_PROSL Carnitinyl-CoA dehydratase (Crotonobetainyl-CoA hydratase)
Length = 261
Score = 78.6 bits (192), Expect = 1e-14
Identities = 50/166 (30%), Positives = 90/166 (54%), Gaps = 4/166 (2%)
Frame = +2
Query: 71 KPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDS 250
KPVIAA++ GG ++ AD+ + + +A F + E LG+ D G + R PK + +
Sbjct: 95 KPVIAAINGYAFGGGFELALAADMIICSDNASFALPEAQLGIVPDSGGVLRLPKRL-PPA 153
Query: 251 LARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALN 430
+ E++ T R+ A+EAL+ G + + E L++ + +LA +IA +P+AV A K
Sbjct: 154 IVNEMLMTGRRMNAQEALRWGIANRVVSATE-LMDSARELADQIANSAPLAVAALKEIYR 212
Query: 431 YSRDHSTEEGLRMQAD----WNQVMLQSEDLMKSIMASIQKEKPQF 556
+ + S EEG ++ + +L SED ++ +A +K P++
Sbjct: 213 ATSELSIEEGYKLMRSGVLKYYPRVLHSEDALEGPLAFAEKRSPEW 258
>sp|Q13825|AUMH_HUMAN Methylglutaconyl-CoA hydratase, mitochondrial precursor
(AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding protein/enoyl-CoA hydratase)
Length = 339
Score = 78.2 bits (191), Expect = 1e-14
Identities = 46/164 (28%), Positives = 85/164 (51%), Gaps = 3/164 (1%)
Frame = +2
Query: 74 PVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSL 253
P IAA+ +GG +++ DIR++ A + E L + G QR P+ IG SL
Sbjct: 174 PTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGM-SL 232
Query: 254 ARELVYTARKFTAEEALKIGFISGIFD---DKEALLNQSFKLAGEIAALSPVAVQASKAA 424
A+EL+++AR +EA +G IS + + + +A ++ LA E PVA++ +K A
Sbjct: 233 AKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLA 292
Query: 425 LNYSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 556
+N + GL ++ + ++D ++ ++A +K P++
Sbjct: 293 INQGMEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRY 336
>sp|Q8Z9L5|CAID_SALTI Carnitinyl-CoA dehydratase (Crotonobetainyl-CoA hydratase)
Length = 261
Score = 76.6 bits (187), Expect = 4e-14
Identities = 50/164 (30%), Positives = 84/164 (51%), Gaps = 4/164 (2%)
Frame = +2
Query: 71 KPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDS 250
KPVIAAV+ GG ++ AD + ++A F + E LG+ D G + R PK++ +
Sbjct: 95 KPVIAAVNGYAFGGGFELALAADFIVCAENASFALPEAKLGIVPDSGGVLRLPKLL-PPA 153
Query: 251 LARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALN 430
+ E+V T R+ +AEEAL+ G ++ + E L+ + +LA ++ +P+A+ A K
Sbjct: 154 IVNEMVMTGRRMSAEEALRWGIVNRVVSQSE-LMESARELAQQLVNSAPLAIAALKEIYR 212
Query: 431 YSRDHSTEEGLRM----QADWNQVMLQSEDLMKSIMASIQKEKP 550
+ + EEG R +L SED ++ A +K P
Sbjct: 213 ATSEMPVEEGYRYIRSGVLKHYPSVLHSEDALEGPQAFAEKRAP 256
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,836,869
Number of Sequences: 369166
Number of extensions: 1458319
Number of successful extensions: 3941
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3782
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3902
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4553075865
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)