Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_L13
(514 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O35459|ECH1_MOUSE Delta3,5-delta2,4-dienoyl-CoA isomeras... 184 8e-47
sp|Q62651|ECH1_RAT Delta3,5-delta2,4-dienoyl-CoA isomerase,... 183 2e-46
sp|Q5RFG0|ECH1_PONPY Delta3,5-delta2,4-dienoyl-CoA isomeras... 177 1e-44
sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Croto... 86 4e-17
sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF 78 1e-14
sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitoch... 73 3e-13
sp|Q13825|AUMH_HUMAN Methylglutaconyl-CoA hydratase, mitoch... 72 6e-13
sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochon... 70 3e-12
sp|Q8GB17|CAID_PROSL Carnitinyl-CoA dehydratase (Crotonobet... 69 5e-12
sp|Q8Z9L5|CAID_SALTI Carnitinyl-CoA dehydratase (Crotonobet... 67 2e-11
>sp|O35459|ECH1_MOUSE Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 327
Score = 184 bits (468), Expect = 8e-47
Identities = 91/163 (55%), Positives = 120/163 (73%), Gaps = 3/163 (1%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
A+H C+GG VD++S DIR T+DA+FQIKEV +GLAADVGTLQR PKVIGN SL EL
Sbjct: 165 AIHGGCIGGGVDLVSACDIRYCTQDAFFQIKEVDMGLAADVGTLQRLPKVIGNQSLVNEL 224
Query: 182 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 361
++ARK A+EAL G +S +F DK+A+LN +F LA +I++ SPVAVQ SK L YSRDH
Sbjct: 225 TFSARKMMADEALDSGLVSRVFQDKDAMLNAAFALAADISSKSPVAVQGSKINLIYSRDH 284
Query: 362 STEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQ---FAKL 481
S +E L A WN MLQ++D++KS+ A+++K + F+KL
Sbjct: 285 SVDESLDYMATWNMSMLQTQDIIKSVQAAMEKRDTKSITFSKL 327
>sp|Q62651|ECH1_RAT Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 327
Score = 183 bits (465), Expect = 2e-46
Identities = 91/163 (55%), Positives = 120/163 (73%), Gaps = 3/163 (1%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
A+H C+GG VD+IS DIR T+DA+FQ+KEV +GLAADVGTLQR PKVIGN SL EL
Sbjct: 165 AIHGGCIGGGVDLISACDIRYCTQDAFFQVKEVDVGLAADVGTLQRLPKVIGNRSLVNEL 224
Query: 182 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 361
+TARK A+EAL G +S +F DK+ +LN +F LA +I++ SPVAVQ SK L YSRDH
Sbjct: 225 TFTARKMMADEALDSGLVSRVFPDKDVMLNAAFALAADISSKSPVAVQGSKINLIYSRDH 284
Query: 362 STEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQ---FAKL 481
S +E L A WN MLQ++D++KS+ A+++K+ + F+KL
Sbjct: 285 SVDESLDYMATWNMSMLQTQDIIKSVQAAMEKKDSKSITFSKL 327
>sp|Q5RFG0|ECH1_PONPY Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 328
Score = 177 bits (449), Expect = 1e-44
Identities = 86/154 (55%), Positives = 112/154 (72%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
AVH C+GG VD+++ DIR +DA+FQ+KEV +GLAADVGTLQR PKVIGN SL EL
Sbjct: 166 AVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNEL 225
Query: 182 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 361
+TARK A+EAL G +S +F DKE +L+ + LA EI++ SPVAVQ++K L YSRDH
Sbjct: 226 AFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDH 285
Query: 362 STEEGLRMQADWNQVMLQSEDLMKSIMASIQKEK 463
S E L A WN MLQ++DLMKS+ A+ + ++
Sbjct: 286 SVAESLNYVASWNMSMLQTQDLMKSVQAATENKE 319
>sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Crotonase)
Length = 261
Score = 86.3 bits (212), Expect = 4e-17
Identities = 51/167 (30%), Positives = 95/167 (56%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
AV+ +GG ++ DIR+++ +A F EVGLG+ G QR +++G +A++L
Sbjct: 103 AVNGFALGGGCEIAMSCDIRIASSNARFGQPEVGLGITPGFGGTQRLSRLVGM-GMAKQL 161
Query: 182 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 361
++TA+ A+EAL+IG ++ + + E L+N + ++A +I + +PVAV+ SK A+N
Sbjct: 162 IFTAQNIKADEALRIGLVNKVVEPSE-LMNTAKEIANKIVSNAPVAVKLSKQAINRGMQC 220
Query: 362 STEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQFAKL*FGYKNK 502
+ L +++ +ED ++ A I+K K + G+KN+
Sbjct: 221 DIDTALAFESEAFGECFSTEDQKDAMTAFIEKRKIE------GFKNR 261
>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
Length = 255
Score = 78.2 bits (191), Expect = 1e-14
Identities = 46/157 (29%), Positives = 86/157 (54%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
AV+ +G ++ D+ ++ ++A F + E+ LG+ G QR + +G SLA ++
Sbjct: 98 AVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGK-SLASKM 156
Query: 182 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 361
V + TA++A + G +S +F + L + +LA ++A SP+A+QA+K AL S++
Sbjct: 157 VLSGESITAQQAQQAGLVSDVFPS-DLTLEYALQLASKMARHSPLALQAAKQALRQSQEV 215
Query: 362 STEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 472
+ + GL + ++ +ED + I A +QK P F
Sbjct: 216 ALQAGLAQERQLFTLLAATEDRHEGISAFLQKRTPDF 252
>sp|Q9JLZ3|AUHM_MOUSE Methylglutaconyl-CoA hydratase, mitochondrial precursor
(AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding enoyl-CoA hydratase) (muAUH)
Length = 314
Score = 73.2 bits (178), Expect = 3e-13
Identities = 43/160 (26%), Positives = 82/160 (51%), Gaps = 3/160 (1%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
A+ +GG +++ DIR++ A + E L + G QR P+ IG SLA+EL
Sbjct: 153 AIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGM-SLAKEL 211
Query: 182 VYTARKFTAEEALKIGFISGIFD---DKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 352
+++AR +EA +G IS + + + +A ++ LA E PVA++ +K A+N
Sbjct: 212 IFSARVLDGQEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQG 271
Query: 353 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 472
+ GL ++ + ++D ++ ++A +K P++
Sbjct: 272 MEVDLVTGLAIEEACYAQTISTKDRLEGLLAFKEKRPPRY 311
>sp|Q13825|AUMH_HUMAN Methylglutaconyl-CoA hydratase, mitochondrial precursor
(AU-specific RNA-binding enoyl-CoA hydratase)
(AU-binding protein/enoyl-CoA hydratase)
Length = 339
Score = 72.4 bits (176), Expect = 6e-13
Identities = 43/160 (26%), Positives = 82/160 (51%), Gaps = 3/160 (1%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
A+ +GG +++ DIR++ A + E L + G QR P+ IG SLA+EL
Sbjct: 178 AIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGM-SLAKEL 236
Query: 182 VYTARKFTAEEALKIGFISGIFD---DKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 352
+++AR +EA +G IS + + + +A ++ LA E PVA++ +K A+N
Sbjct: 237 IFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQG 296
Query: 353 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 472
+ GL ++ + ++D ++ ++A +K P++
Sbjct: 297 MEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRY 336
>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
Length = 288
Score = 70.1 bits (170), Expect = 3e-12
Identities = 46/157 (29%), Positives = 80/157 (50%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
AV+ +GG ++ DI + + A F E+ +G G QR+ + G S A E+
Sbjct: 131 AVNGFALGGGNELAMMCDIIYAGEKARFGQPEINIGTIPGAGGTQRWARAAGK-SFAMEV 189
Query: 182 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 361
T TA+EA + G +S IF + ++ ++ KL +IA SP+ VQ +K A+N + +
Sbjct: 190 CLTGNHVTAQEAKEHGIVSKIFP-ADQVVGEAVKLGEKIADQSPLIVQMAKEAVNKAYEL 248
Query: 362 STEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 472
+ +EGL + ++D + + A +K KPQ+
Sbjct: 249 TLQEGLHFERRLFHATFATKDRKEGMTAFAEKRKPQW 285
>sp|Q8GB17|CAID_PROSL Carnitinyl-CoA dehydratase (Crotonobetainyl-CoA hydratase)
Length = 261
Score = 69.3 bits (168), Expect = 5e-12
Identities = 45/161 (27%), Positives = 85/161 (52%), Gaps = 4/161 (2%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
A++ GG ++ AD+ + + +A F + E LG+ D G + R PK + ++ E+
Sbjct: 100 AINGYAFGGGFELALAADMIICSDNASFALPEAQLGIVPDSGGVLRLPKRL-PPAIVNEM 158
Query: 182 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 361
+ T R+ A+EAL+ G + + E L++ + +LA +IA +P+AV A K + +
Sbjct: 159 LMTGRRMNAQEALRWGIANRVVSATE-LMDSARELADQIANSAPLAVAALKEIYRATSEL 217
Query: 362 STEEGLRMQAD----WNQVMLQSEDLMKSIMASIQKEKPQF 472
S EEG ++ + +L SED ++ +A +K P++
Sbjct: 218 SIEEGYKLMRSGVLKYYPRVLHSEDALEGPLAFAEKRSPEW 258
>sp|Q8Z9L5|CAID_SALTI Carnitinyl-CoA dehydratase (Crotonobetainyl-CoA hydratase)
Length = 261
Score = 67.4 bits (163), Expect = 2e-11
Identities = 45/159 (28%), Positives = 79/159 (49%), Gaps = 4/159 (2%)
Frame = +2
Query: 2 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 181
AV+ GG ++ AD + ++A F + E LG+ D G + R PK++ ++ E+
Sbjct: 100 AVNGYAFGGGFELALAADFIVCAENASFALPEAKLGIVPDSGGVLRLPKLL-PPAIVNEM 158
Query: 182 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 361
V T R+ +AEEAL+ G ++ + E L+ + +LA ++ +P+A+ A K + +
Sbjct: 159 VMTGRRMSAEEALRWGIVNRVVSQSE-LMESARELAQQLVNSAPLAIAALKEIYRATSEM 217
Query: 362 STEEGLRM----QADWNQVMLQSEDLMKSIMASIQKEKP 466
EEG R +L SED ++ A +K P
Sbjct: 218 PVEEGYRYIRSGVLKHYPSVLHSEDALEGPQAFAEKRAP 256
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,640,106
Number of Sequences: 369166
Number of extensions: 1251696
Number of successful extensions: 3333
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3241
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3306
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3304927425
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)