Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_N18
(704 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O35459|ECH1_MOUSE Delta3,5-delta2,4-dienoyl-CoA isomeras... 243 3e-64
sp|Q62651|ECH1_RAT Delta3,5-delta2,4-dienoyl-CoA isomerase,... 240 3e-63
sp|Q5RFG0|ECH1_PONPY Delta3,5-delta2,4-dienoyl-CoA isomeras... 233 3e-61
sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Croto... 110 3e-24
sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF 93 8e-19
sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochon... 89 1e-17
sp|Q8BH95|ECHM_MOUSE Enoyl-CoA hydratase, mitochondrial pre... 86 1e-16
sp|Q50130|ECHA6_MYCLE Probable enoyl-CoA hydratase echA6 86 1e-16
sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial pre... 85 2e-16
sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precu... 83 6e-16
>sp|O35459|ECH1_MOUSE Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 327
Score = 243 bits (621), Expect = 3e-64
Identities = 123/226 (54%), Positives = 163/226 (72%), Gaps = 3/226 (1%)
Frame = +3
Query: 3 RCVVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKP 182
R VV+SGAGK+F++GIDL +M L +D +R A+ +R LI YQ +FT +E C KP
Sbjct: 102 RAVVVSGAGKMFTSGIDLMDMASELMQPSGDDAARIAWYLRDLISKYQKTFTVIEKCPKP 161
Query: 183 VIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLA 362
VIAA+H C+GG VD++S DIR T+DA+FQIKEV +GLAADVGTLQR PKVIGN SL
Sbjct: 162 VIAAIHGGCIGGGVDLVSACDIRYCTQDAFFQIKEVDMGLAADVGTLQRLPKVIGNQSLV 221
Query: 363 RELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 542
EL ++ARK A+EAL G +S +F DK+A+LN +F LA +I++ SPVAVQ SK L YS
Sbjct: 222 NELTFSARKMMADEALDSGLVSRVFQDKDAMLNAAFALAADISSKSPVAVQGSKINLIYS 281
Query: 543 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQ---FAKL 671
RDHS +E L A WN MLQ++D++KS+ A+++K + F+KL
Sbjct: 282 RDHSVDESLDYMATWNMSMLQTQDIIKSVQAAMEKRDTKSITFSKL 327
>sp|Q62651|ECH1_RAT Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 327
Score = 240 bits (612), Expect = 3e-63
Identities = 122/226 (53%), Positives = 163/226 (72%), Gaps = 3/226 (1%)
Frame = +3
Query: 3 RCVVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKP 182
R VV+SGAGK+F++GIDL +M + +D +R A+ +R LI YQ +FT +E C KP
Sbjct: 102 RAVVVSGAGKMFTSGIDLMDMASDILQPPGDDVARIAWYLRDLISRYQKTFTVIEKCPKP 161
Query: 183 VIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLA 362
VIAA+H C+GG VD+IS DIR T+DA+FQ+KEV +GLAADVGTLQR PKVIGN SL
Sbjct: 162 VIAAIHGGCIGGGVDLISACDIRYCTQDAFFQVKEVDVGLAADVGTLQRLPKVIGNRSLV 221
Query: 363 RELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 542
EL +TARK A+EAL G +S +F DK+ +LN +F LA +I++ SPVAVQ SK L YS
Sbjct: 222 NELTFTARKMMADEALDSGLVSRVFPDKDVMLNAAFALAADISSKSPVAVQGSKINLIYS 281
Query: 543 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQ---FAKL 671
RDHS +E L A WN MLQ++D++KS+ A+++K+ + F+KL
Sbjct: 282 RDHSVDESLDYMATWNMSMLQTQDIIKSVQAAMEKKDSKSITFSKL 327
>sp|Q5RFG0|ECH1_PONPY Delta3,5-delta2,4-dienoyl-CoA isomerase, mitochondrial precursor
Length = 328
Score = 233 bits (595), Expect = 3e-61
Identities = 115/217 (52%), Positives = 155/217 (71%)
Frame = +3
Query: 3 RCVVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKP 182
R VV+SGAGK+F+AG+DL +M + K +D +R ++ +R +I YQ +F +E C KP
Sbjct: 103 RAVVISGAGKMFTAGVDLMDMASDILQPKGDDVARISWYLRDIITRYQETFNVIEKCPKP 162
Query: 183 VIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLA 362
VIAAVH C+GG VD+++ DIR +DA+FQ+KEV +GLAADVGTLQR PKVIGN SL
Sbjct: 163 VIAAVHGGCIGGGVDLVTACDIRYCAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLV 222
Query: 363 RELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 542
EL +TARK A+EAL G +S +F DKE +L+ + LA EI++ SPVAVQ++K L YS
Sbjct: 223 NELAFTARKMMADEALGSGLVSRVFPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYS 282
Query: 543 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEK 653
RDHS E L A WN MLQ++DLMKS+ A+ + ++
Sbjct: 283 RDHSVAESLNYVASWNMSMLQTQDLMKSVQAATENKE 319
>sp|P52046|CRT_CLOAB 3-hydroxybutyryl-CoA dehydratase (Crotonase)
Length = 261
Score = 110 bits (276), Expect = 3e-24
Identities = 74/229 (32%), Positives = 126/229 (55%), Gaps = 1/229 (0%)
Frame = +3
Query: 9 VVLSGAG-KVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPV 185
V+L+GAG K F AG D+SEM ++ + R+ + F LEL KPV
Sbjct: 52 VILTGAGEKSFVAGADISEMKEM-----------NTIEGRKFGILGNKVFRRLELLEKPV 100
Query: 186 IAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAR 365
IAAV+ +GG ++ DIR+++ +A F EVGLG+ G QR +++G +A+
Sbjct: 101 IAAVNGFALGGGCEIAMSCDIRIASSNARFGQPEVGLGITPGFGGTQRLSRLVGM-GMAK 159
Query: 366 ELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSR 545
+L++TA+ A+EAL+IG ++ + + E L+N + ++A +I + +PVAV+ SK A+N
Sbjct: 160 QLIFTAQNIKADEALRIGLVNKVVEPSE-LMNTAKEIANKIVSNAPVAVKLSKQAINRGM 218
Query: 546 DHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQFAKL*FGYKNK 692
+ L +++ +ED ++ A I+K K + G+KN+
Sbjct: 219 QCDIDTALAFESEAFGECFSTEDQKDAMTAFIEKRKIE------GFKNR 261
>sp|P76082|PAAF_ECOLI Probable enoyl-CoA hydratase paaF
Length = 255
Score = 92.8 bits (229), Expect = 8e-19
Identities = 62/217 (28%), Positives = 113/217 (52%)
Frame = +3
Query: 12 VLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPVIA 191
V++G + F+AG DL+EM + +D + R + +F KP+IA
Sbjct: 51 VITGNARFFAAGADLNEMAE-------KDLAATLNDTRPQLWARLQAFN------KPLIA 97
Query: 192 AVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLAREL 371
AV+ +G ++ D+ ++ ++A F + E+ LG+ G QR + +G SLA ++
Sbjct: 98 AVNGYALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGK-SLASKM 156
Query: 372 VYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRDH 551
V + TA++A + G +S +F + L + +LA ++A SP+A+QA+K AL S++
Sbjct: 157 VLSGESITAQQAQQAGLVSDVFPS-DLTLEYALQLASKMARHSPLALQAAKQALRQSQEV 215
Query: 552 STEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
+ + GL + ++ +ED + I A +QK P F
Sbjct: 216 ALQAGLAQERQLFTLLAATEDRHEGISAFLQKRTPDF 252
>sp|P34559|ECHM_CAEEL Probable enoyl-CoA hydratase, mitochondrial
Length = 288
Score = 88.6 bits (218), Expect = 1e-17
Identities = 62/218 (28%), Positives = 111/218 (50%)
Frame = +3
Query: 9 VVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPVI 188
+V++G+ + F+AG D+ EM + + F ++ S++T++ KPVI
Sbjct: 83 IVITGSERAFAAGADIKEM--------TNNEFATTFS-----GSFLSNWTAVSDVKKPVI 129
Query: 189 AAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLARE 368
AAV+ +GG ++ DI + + A F E+ +G G QR+ + G S A E
Sbjct: 130 AAVNGFALGGGNELAMMCDIIYAGEKARFGQPEINIGTIPGAGGTQRWARAAGK-SFAME 188
Query: 369 LVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRD 548
+ T TA+EA + G +S IF + ++ ++ KL +IA SP+ VQ +K A+N + +
Sbjct: 189 VCLTGNHVTAQEAKEHGIVSKIFP-ADQVVGEAVKLGEKIADQSPLIVQMAKEAVNKAYE 247
Query: 549 HSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
+ +EGL + ++D + + A +K KPQ+
Sbjct: 248 LTLQEGLHFERRLFHATFATKDRKEGMTAFAEKRKPQW 285
>sp|Q8BH95|ECHM_MOUSE Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 85.5 bits (210), Expect = 1e-16
Identities = 65/222 (29%), Positives = 108/222 (48%), Gaps = 4/222 (1%)
Frame = +3
Query: 9 VVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTS----LELCL 176
+VL+G K F+AG D+ EM R FQ Y S F S +
Sbjct: 85 IVLTGGDKAFAAGADIKEM------------QNRTFQDC-----YSSKFLSHWDHITRVK 127
Query: 177 KPVIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDS 356
KPVIAAV+ +GG ++ DI + + A F E+ LG G QR + +G S
Sbjct: 128 KPVIAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGK-S 186
Query: 357 LARELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALN 536
LA E+V T + +A++A + G +S IF E L+ ++ + A +IA+ S + V +K ++N
Sbjct: 187 LAMEMVLTGDRISAQDAKQAGLVSKIF-PVEKLVEEAIQCAEKIASNSKIVVAMAKESVN 245
Query: 537 YSRDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
+ + + EG +++ ++D + + A ++K K F
Sbjct: 246 AAFEMTLTEGNKLEKRLFYSTFATDDRREGMTAFVEKRKANF 287
>sp|Q50130|ECHA6_MYCLE Probable enoyl-CoA hydratase echA6
Length = 247
Score = 85.5 bits (210), Expect = 1e-16
Identities = 64/220 (29%), Positives = 106/220 (48%)
Frame = +3
Query: 3 RCVVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKP 182
R +VL+G G VF AG DLS G + D R ++ R+ ++ P
Sbjct: 49 RVIVLTGQGTVFCAGADLS------GDAFAADYPDRLIELHRV----------MDAVPMP 92
Query: 183 VIAAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLA 362
VI A++ +G + + D+R+ DA+FQ GLA D +++R ++G+ A
Sbjct: 93 VIGAINGPAIGAGLQLAMQCDLRVVAPDAYFQFPTSKYGLALDNWSIRRLSSLVGH-GRA 151
Query: 363 RELVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYS 542
R ++ TA K TA+ AL+ G + I L + A E+ L+P+A+Q +K LN
Sbjct: 152 RAMLLTAEKLTADIALQTGMANRI-----GALADAQAWAAEVTGLAPLAIQHAKRVLN-- 204
Query: 543 RDHSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
D S EE S+D++++ +A ++K P+F
Sbjct: 205 DDGSIEEAWPEHKKLFDKAWTSQDVIEAQVARVEKRPPKF 244
>sp|Q58DM8|ECHM_BOVIN Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 85.1 bits (209), Expect = 2e-16
Identities = 61/218 (27%), Positives = 108/218 (49%)
Frame = +3
Query: 9 VVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPVI 188
+VL+G KVF+AG D+ EM + FQ + S + L KPVI
Sbjct: 85 IVLTGGEKVFAAGADIKEMQSL------------TFQ-NCYSGGFLSHWDQLTRVKKPVI 131
Query: 189 AAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLARE 368
AAV+ +GG ++ DI + + A F E+ +G G QR + +G SLA E
Sbjct: 132 AAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILIGTIPGAGGTQRLTRAVGK-SLAME 190
Query: 369 LVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRD 548
+V T + +A++A + G +S IF E ++ ++ + A +IA+ S + +K ++N + +
Sbjct: 191 MVLTGDRISAQDAKQAGLVSKIF-PVETVVEEAIQCAEKIASNSKIVTAMAKESVNAAFE 249
Query: 549 HSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
+ EG++++ +ED + + A ++K K F
Sbjct: 250 MTLAEGVKLEKKLFYSTFATEDRKEGMAAFVEKRKANF 287
>sp|P14604|ECHM_RAT Enoyl-CoA hydratase, mitochondrial precursor (Short chain enoyl-CoA
hydratase) (SCEH) (Enoyl-CoA hydratase 1)
Length = 290
Score = 83.2 bits (204), Expect = 6e-16
Identities = 62/218 (28%), Positives = 106/218 (48%)
Frame = +3
Query: 9 VVLSGAGKVFSAGIDLSEMGQVLGSIKSEDPSRRAFQIRRLIMNYQSSFTSLELCLKPVI 188
+VL+G K F+AG D+ EM R FQ + S + + KPVI
Sbjct: 85 IVLTGGEKAFAAGADIKEM------------QNRTFQ-DCYSGKFLSHWDHITRIKKPVI 131
Query: 189 AAVHNACVGGAVDMISCADIRMSTKDAWFQIKEVGLGLAADVGTLQRFPKVIGNDSLARE 368
AAV+ +GG ++ DI + + A F E+ LG G QR + +G SLA E
Sbjct: 132 AAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGK-SLAME 190
Query: 369 LVYTARKFTAEEALKIGFISGIFDDKEALLNQSFKLAGEIAALSPVAVQASKAALNYSRD 548
+V T + +A++A + G +S IF E L+ ++ + A +IA S + V +K ++N + +
Sbjct: 191 MVLTGDRISAQDAKQAGLVSKIF-PVETLVEEAIQCAEKIANNSKIIVAMAKESVNAAFE 249
Query: 549 HSTEEGLRMQADWNQVMLQSEDLMKSIMASIQKEKPQF 662
+ EG +++ ++D + + A ++K K F
Sbjct: 250 MTLTEGNKLEKKLFYSTFATDDRREGMSAFVEKRKANF 287
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,713,890
Number of Sequences: 369166
Number of extensions: 1793173
Number of successful extensions: 4952
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4907
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6170718545
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)