Planaria EST Database

DrC_00608

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00608
         (874 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P34714|SPRC_CAEEL  SPARC precursor (Secreted protein acid...    63   1e-09
sp|P23499|SPRL1_COTJA  SPARC-like protein 1 precursor (QR1 p...    56   1e-07
sp|Q14515|SPRL1_HUMAN  SPARC-like protein 1 precursor (High ...    55   2e-07
sp|P70663|SPRL1_MOUSE  SPARC-like protein 1 precursor (Matri...    52   2e-06
sp|P24054|SPRL1_RAT  SPARC-like protein 1 precursor (Matrix ...    51   4e-06
sp|P16975|SPRC_RAT  SPARC precursor (Secreted protein acidic...    49   2e-05
sp|P07214|SPRC_MOUSE  SPARC precursor (Secreted protein acid...    47   7e-05
sp|P36378|SPRC_XENLA  SPARC precursor (Secreted protein acid...    47   9e-05
sp|P09486|SPRC_HUMAN  SPARC precursor (Secreted protein acid...    45   3e-04
sp|P13213|SPRC_BOVIN  SPARC precursor (Secreted protein acid...    45   3e-04

>sp|P34714|SPRC_CAEEL SPARC precursor (Secreted protein acidic and rich in cysteine)
           (Osteonectin) (ON) (Basement-membrane protein 40)
           (BM-40)
          Length = 264

 Score = 62.8 bits (151), Expect = 1e-09
 Identities = 56/245 (22%), Positives = 95/245 (38%), Gaps = 22/245 (8%)
 Frame = +1

Query: 64  APDQCASVQCKDGELCEAGK-----CKCMDQCPEEWNNYPRQLCVDG-KTYRHECDLWRN 225
           A + C   QC  G+ C  GK     C+C+ +CPE   +   ++C +  +T+   CDL+R 
Sbjct: 49  AKNPCEDHQCGWGKECVVGKKGEPTCECISKCPELDGDPMDKVCANNNQTFTSLCDLYRE 108

Query: 226 KCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEE--ASFHARLAMWF- 396
           +C C +    C      S      + ++Y   C+ L    D  +EE  A F  R+A W  
Sbjct: 109 RCLCKRKSKEC------SKAFNAKVHLEYLGECKKL----DECTEEHMAQFPERMADWLF 158

Query: 397 ---------RDLYSQKWSVASGKXXXXXXXXXXXXKTRSSATTLFNAQTSSPYGVHGGYL 549
                    R+L+  +W                          L +   +     H   +
Sbjct: 159 QVMKELKKRRELHKLEWE------------------------ELLSEAENDDEKKHVYPV 194

Query: 550 SFWFCEMD-KNNNGNLDRSEIALLSQLLNPSTPCLNTFLSKC---GSGTISFSAWNSCFK 717
            + FCE+D K ++ ++   E+  ++  + P   C+  FL  C     G IS   W  C  
Sbjct: 195 IWKFCELDTKPHDKSVSHHELIPITAPVIPMESCIKPFLEGCDANNDGNISIKEWGKCLG 254

Query: 718 VPKAE 732
           + + E
Sbjct: 255 LKEGE 259

>sp|P23499|SPRL1_COTJA SPARC-like protein 1 precursor (QR1 protein)
          Length = 676

 Score = 55.8 bits (133), Expect = 1e-07
 Identities = 58/254 (22%), Positives = 94/254 (37%), Gaps = 27/254 (10%)
 Frame = +1

Query: 52   TSTAAPDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGKTYRHEC 210
            T  A PD C +  CK G++C+A K     C C D   CP    +Y R    D KTY   C
Sbjct: 437  TGKALPDLCRNFHCKRGKVCQADKQGKPSCICQDPAACPST-KDYKRVCGTDNKTYDGTC 495

Query: 211  DLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAM 390
             L+  KC       +  G++ G       + + Y   C+ +    D+  E   F  R+  
Sbjct: 496  QLFGTKC-------QLEGTKMGRQ-----LHLDYMGACKHIPHCTDY--EVNQFPLRMRD 541

Query: 391  WFRDLYSQKWSVASGKXXXXXXXXXXXXKTRSSATTLFNAQTSSPYGVHGG--------- 543
            W +++  Q +                  K R+    ++  +     G H           
Sbjct: 542  WLKNILMQYYE------RDQDTSAFLTEKQRNKVKKIYLNEKRLVSGEHPVELLLHDFEK 595

Query: 544  ------YLSFW-FCEMDKNN-NGNLDRSEIALLSQLLNPSTPCLNTFLSKCGSG---TIS 690
                  Y   W F ++D++  + +L  SE+A L   L P   C+  F  +C       I+
Sbjct: 596  NYHMYLYPVHWQFYQLDQHPVDRSLTHSELAPLRASLVPMEHCITRFFQECDGDQDKLIT 655

Query: 691  FSAWNSCFKVPKAE 732
               W  CF + + +
Sbjct: 656  LKEWCHCFAIKEED 669

>sp|Q14515|SPRL1_HUMAN SPARC-like protein 1 precursor (High endothelial venule protein)
            (Hevin) (MAST 9)
          Length = 664

 Score = 55.5 bits (132), Expect = 2e-07
 Identities = 55/243 (22%), Positives = 87/243 (35%), Gaps = 20/243 (8%)
 Frame = +1

Query: 64   APDQCASVQCKDGELCEAGK-----CKCMDQCPEEWNNYPRQLC-VDGKTYRHECDLWRN 225
            A D C S QCK G +C+A +     C C D           Q+C  D +TY   C L+  
Sbjct: 429  AVDSCMSFQCKRGHICKADQQGKPHCVCQDPVTCPPTKPLDQVCGTDNQTYASSCHLFAT 488

Query: 226  KCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAMWFRDL 405
            KC       R  G++ G       + + YF  C+ +    D+  E   F  R+  W +++
Sbjct: 489  KC-------RLEGTKKGHQ-----LQLDYFGACKSIPTCTDF--EVIQFPLRMRDWLKNI 534

Query: 406  YSQKWSVASGKXXXXXXXXXXXXK----------TRSSATTLFNAQTSSPYGVHGGYLSF 555
              Q +   S              K                 L        Y ++   + +
Sbjct: 535  LMQLYEANSEHAGYLNEKQRNKVKKIYLDEKRLLAGDHPIDLLLRDFKKNYHMYVYPVHW 594

Query: 556  WFCEMDKNNNGN-LDRSEIALLSQLLNPSTPCLNTFLSKCGSGT---ISFSAWNSCFKVP 723
             F E+D++     L  SE+A L   L P   C+  F  +C       I+   W  CF + 
Sbjct: 595  QFSELDQHPMDRVLTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIK 654

Query: 724  KAE 732
            + +
Sbjct: 655  EED 657

>sp|P70663|SPRL1_MOUSE SPARC-like protein 1 precursor (Matrix glycoprotein Sc1)
            (Extracellular matrix protein 2)
          Length = 650

 Score = 52.4 bits (124), Expect = 2e-06
 Identities = 59/245 (24%), Positives = 90/245 (36%), Gaps = 26/245 (10%)
 Frame = +1

Query: 76   CASVQCKDGELCEA---GKCKCMDQCPEEW--NNYPRQLC-VDGKTYRHECDLWRNKCYC 237
            C + QCK G +C+    GK  C+ Q PE         Q C  D +TY   C L+  KC  
Sbjct: 419  CTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKC-- 476

Query: 238  AKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAMWFRDLYSQK 417
                 R  G++ G       + + YF  C+ + A  D+  E A F  R+  W +++  Q 
Sbjct: 477  -----RLEGTKKGHQ-----LQLDYFGACKSIPACTDF--EVAQFPLRMRDWLKNILMQL 524

Query: 418  WSVASGKXXXXXXXXXXXXKTRSSATTLFNAQTSSPYGVHG---------------GYLS 552
            +                  K RS    ++  +     G H                 Y  
Sbjct: 525  YE------PNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPV 578

Query: 553  FW-FCEMDKNNNGN-LDRSEIALLSQLLNPSTPCLNTFLSKCGSGT---ISFSAWNSCFK 717
             W F E+D++     L  SE+A L   L P   C+  F  +C       I+   W  CF 
Sbjct: 579  HWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFG 638

Query: 718  VPKAE 732
            + + +
Sbjct: 639  IKEED 643

>sp|P24054|SPRL1_RAT SPARC-like protein 1 precursor (Matrix glycoprotein Sc1)
          Length = 634

 Score = 51.2 bits (121), Expect = 4e-06
 Identities = 58/247 (23%), Positives = 87/247 (35%), Gaps = 26/247 (10%)
 Frame = +1

Query: 70   DQCASVQCKDGELCEA---GKCKCMDQCPEEW--NNYPRQLC-VDGKTYRHECDLWRNKC 231
            D C + QCK G  C+    GK  C+ Q PE         Q C  D +TY   C L+  KC
Sbjct: 401  DSCMNFQCKRGHTCKTDQHGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKC 460

Query: 232  YCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAMWFRDLYS 411
                +            K    + + YF  C+ + A  D+  E A F  R+  W +++  
Sbjct: 461  MLEGT------------KKGHQLQLDYFGACKSIPACTDF--EVAQFPLRMRDWLKNILM 506

Query: 412  QKWSVASGKXXXXXXXXXXXXKTRSSATTLFNAQTSSPYGVHG---------------GY 546
            Q +                  K RS    ++  +     G H                 Y
Sbjct: 507  QLYE------PNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVY 560

Query: 547  LSFW-FCEMDKNNNGN-LDRSEIALLSQLLNPSTPCLNTFLSKCGSGT---ISFSAWNSC 711
               W F E+D++     L  SE+A L   L P   C+  F  +C       I+   W  C
Sbjct: 561  PVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHC 620

Query: 712  FKVPKAE 732
            F + + +
Sbjct: 621  FGIKEED 627

>sp|P16975|SPRC_RAT SPARC precursor (Secreted protein acidic and rich in cysteine)
           (Osteonectin) (ON) (Basement-membrane protein 40)
           (BM-40)
          Length = 302

 Score = 48.9 bits (115), Expect = 2e-05
 Identities = 52/252 (20%), Positives = 91/252 (36%), Gaps = 20/252 (7%)
 Frame = +1

Query: 37  QKVLETSTAAPDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGKT 195
           ++ +E   A  + C +  CK G++CE  +     C C D   CP     + +    D KT
Sbjct: 58  EETVEEVVACNNPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNKT 117

Query: 196 YRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFH 375
           +   C  +  KC          G++ G HK    + + Y   C+ +A   D  SE   F 
Sbjct: 118 FDSSCHFFATKC-------TLEGTKKG-HK----LHLDYIGPCKYIAPCLD--SELTEFP 163

Query: 376 ARLAMWFRDLYSQKWSVASGKXXXXXXXXXXXXKTRSS---------ATTLFNAQTSSPY 528
            R+  W +++    +    G             K   +            L        Y
Sbjct: 164 LRMRDWLKNVLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNY 223

Query: 529 GVHGGYLSFWFCEMDKNN-NGNLDRSEIALLSQLLNPSTPCLNTFLSKC---GSGTISFS 696
            ++   + + F ++D++  +G L  +E+A L   L P   C   F   C       I+  
Sbjct: 224 NMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALE 283

Query: 697 AWNSCFKVPKAE 732
            W  CF + + +
Sbjct: 284 EWAGCFGIKEQD 295

>sp|P07214|SPRC_MOUSE SPARC precursor (Secreted protein acidic and rich in cysteine)
           (Osteonectin) (ON) (Basement-membrane protein 40)
           (BM-40)
          Length = 302

 Score = 47.0 bits (110), Expect = 7e-05
 Identities = 51/243 (20%), Positives = 87/243 (35%), Gaps = 20/243 (8%)
 Frame = +1

Query: 64  APDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGKTYRHECDLWR 222
           A + C +  CK G++CE  +     C C D   CP     + +    D KT+   C  + 
Sbjct: 67  ADNPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFA 126

Query: 223 NKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAMWFRD 402
            KC          G++ G HK    + + Y   C+ +A   D  SE   F  R+  W ++
Sbjct: 127 TKC-------TLEGTKKG-HK----LHLDYIGPCKYIAPCLD--SELTEFPLRMRDWLKN 172

Query: 403 LYSQKWSVASGKXXXXXXXXXXXXKTRSS---------ATTLFNAQTSSPYGVHGGYLSF 555
           +    +    G             K   +            L        Y ++   + +
Sbjct: 173 VLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHW 232

Query: 556 WFCEMDKNN-NGNLDRSEIALLSQLLNPSTPCLNTFLSKC---GSGTISFSAWNSCFKVP 723
            F ++D++  +G L  +E+A L   L P   C   F   C       I+   W  CF + 
Sbjct: 233 QFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALEEWAGCFGIK 292

Query: 724 KAE 732
           + +
Sbjct: 293 EQD 295

>sp|P36378|SPRC_XENLA SPARC precursor (Secreted protein acidic and rich in cysteine)
           (Osteonectin) (ON) (Basement-membrane protein 40)
           (BM-40)
          Length = 300

 Score = 46.6 bits (109), Expect = 9e-05
 Identities = 50/248 (20%), Positives = 92/248 (37%), Gaps = 20/248 (8%)
 Frame = +1

Query: 49  ETSTAAPDQCASVQCKDGELCEAGK-----CKCMDQ--CPEEWNNYPRQLCVDGKTYRHE 207
           E    + + C +  CK G++CE  +     C C D   CP     + +    D KTY   
Sbjct: 60  EEEEPSENPCLNHHCKHGKVCEVDESNTPLCVCQDPSTCPTSVGEFEKICGTDNKTYDSS 119

Query: 208 CDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLA 387
           C  +  KC          G++ G HK    + + Y   C+ +A   D  +E + F  R+ 
Sbjct: 120 CHFFATKC-------TLEGTKKG-HK----LHLDYIGPCKYIAPCLD--NELSEFPLRIG 165

Query: 388 MWFRDLYSQKWSVASGKXXXXXXXXXXXXKTRSSATTLFNAQTSSP---------YGVHG 540
            W +++    +                  K   +   L + +  S          Y ++ 
Sbjct: 166 DWLKNVLVSLYERDENNNLLNEKQKLRVKKIHENEKRLESWRPHSELLVRDFEKNYNMYI 225

Query: 541 GYLSFWFCEMDKNN-NGNLDRSEIALLSQLLNPSTPCLNTFLSKC---GSGTISFSAWNS 708
             + + F ++D++  +G L  +E++ L   L P   C   F  +C       I+   W  
Sbjct: 226 FPVHWQFGQLDQHPIDGYLSHTELSPLRAPLIPMEHCTTRFFDECDIDDDKYIALEEWAK 285

Query: 709 CFKVPKAE 732
           CF + + +
Sbjct: 286 CFGIKEQD 293

>sp|P09486|SPRC_HUMAN SPARC precursor (Secreted protein acidic and rich in cysteine)
           (Osteonectin) (ON) (Basement-membrane protein 40)
           (BM-40)
          Length = 303

 Score = 45.1 bits (105), Expect = 3e-04
 Identities = 51/253 (20%), Positives = 88/253 (34%), Gaps = 20/253 (7%)
 Frame = +1

Query: 34  GQKVLETSTAAPDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGK 192
           G +  E    A + C +  CK G++CE  +     C C D   CP     + +    D K
Sbjct: 58  GAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNK 117

Query: 193 TYRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASF 372
           T+   C  +  KC          G++ G HK    + + Y   C+ +    D  SE   F
Sbjct: 118 TFDSSCHFFATKC-------TLEGTKKG-HK----LHLDYIGPCKYIPPCLD--SELTEF 163

Query: 373 HARLAMWFRDLYSQKWSVASGKXXXXXXXXXXXXKTRSS---------ATTLFNAQTSSP 525
             R+  W +++    +                  K   +            L        
Sbjct: 164 PLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKN 223

Query: 526 YGVHGGYLSFWFCEMDKNN-NGNLDRSEIALLSQLLNPSTPCLNTFLSKC---GSGTISF 693
           Y ++   + + F ++D++  +G L  +E+A L   L P   C   F   C       I+ 
Sbjct: 224 YNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIAL 283

Query: 694 SAWNSCFKVPKAE 732
             W  CF + + +
Sbjct: 284 DEWAGCFGIKQKD 296

>sp|P13213|SPRC_BOVIN SPARC precursor (Secreted protein acidic and rich in cysteine)
           (Osteonectin) (ON) (Basement-membrane protein 40)
           (BM-40)
          Length = 304

 Score = 44.7 bits (104), Expect = 3e-04
 Identities = 51/254 (20%), Positives = 88/254 (34%), Gaps = 21/254 (8%)
 Frame = +1

Query: 34  GQKVLETSTAAPDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGK 192
           G +  E    A + C +  CK G++CE  +     C C D   CP     + +    D K
Sbjct: 58  GAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNK 117

Query: 193 TYRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASF 372
           T+   C  +  KC          G++ G HK    + + Y   C+ +    D  SE   F
Sbjct: 118 TFDSSCHFFATKC-------TLEGTKKG-HK----LHLDYIGPCKYIPPCLD--SELTEF 163

Query: 373 HARLAMWFRDLYSQKWSVASGKXXXXXXXXXXXXKTRSS----------ATTLFNAQTSS 522
             R+  W +++    +                  K   +             L       
Sbjct: 164 PLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGCDHPVELLARDFEK 223

Query: 523 PYGVHGGYLSFWFCEMDKNN-NGNLDRSEIALLSQLLNPSTPCLNTFLSKC---GSGTIS 690
            Y ++   + + F ++D++  +G L  +E+A L   L P   C   F   C       I+
Sbjct: 224 NYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIA 283

Query: 691 FSAWNSCFKVPKAE 732
              W  CF + + +
Sbjct: 284 LDEWAGCFGIKEKD 297

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 101,227,864
Number of Sequences: 369166
Number of extensions: 2077421
Number of successful extensions: 5364
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4925
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5323
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8646143400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00608

  1. Dr_sW_015_P13
  2. Dr_sW_010_P11
  3. Dr_sW_002_G21
  4. Dr_sW_026_H24