Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_P13
(462 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P34714|SPRC_CAEEL SPARC precursor (Secreted protein acid... 53 3e-07
sp|P23499|SPRL1_COTJA SPARC-like protein 1 precursor (QR1 p... 50 2e-06
sp|Q14515|SPRL1_HUMAN SPARC-like protein 1 precursor (High ... 48 9e-06
sp|P70663|SPRL1_MOUSE SPARC-like protein 1 precursor (Matri... 46 4e-05
sp|P24054|SPRL1_RAT SPARC-like protein 1 precursor (Matrix ... 45 8e-05
sp|P16975|SPRC_RAT SPARC precursor (Secreted protein acidic... 42 9e-04
sp|P09486|SPRC_HUMAN SPARC precursor (Secreted protein acid... 41 0.001
sp|P13213|SPRC_BOVIN SPARC precursor (Secreted protein acid... 41 0.001
sp|P36378|SPRC_XENLA SPARC precursor (Secreted protein acid... 41 0.001
sp|P07214|SPRC_MOUSE SPARC precursor (Secreted protein acid... 40 0.003
>sp|P34714|SPRC_CAEEL SPARC precursor (Secreted protein acidic and rich in cysteine)
(Osteonectin) (ON) (Basement-membrane protein 40)
(BM-40)
Length = 264
Score = 53.1 bits (126), Expect = 3e-07
Identities = 39/149 (26%), Positives = 67/149 (44%), Gaps = 20/149 (13%)
Frame = +1
Query: 64 APDQCASVQCKDGELCEAGK-----CKCMDQCPEEWNNYPRQLCVDG-KTYRHECDLWRN 225
A + C QC G+ C GK C+C+ +CPE + ++C + +T+ CDL+R
Sbjct: 49 AKNPCEDHQCGWGKECVVGKKGEPTCECISKCPELDGDPMDKVCANNNQTFTSLCDLYRE 108
Query: 226 KCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEE--ASFHARLAMWF- 396
+C C + C S + ++Y C+ L D +EE A F R+A W
Sbjct: 109 RCLCKRKSKEC------SKAFNAKVHLEYLGECKKL----DECTEEHMAQFPERMADWLF 158
Query: 397 ---------RDLYSQKWS--VASGKSDDQ 450
R+L+ +W ++ ++DD+
Sbjct: 159 QVMKELKKRRELHKLEWEELLSEAENDDE 187
>sp|P23499|SPRL1_COTJA SPARC-like protein 1 precursor (QR1 protein)
Length = 676
Score = 50.4 bits (119), Expect = 2e-06
Identities = 36/128 (28%), Positives = 54/128 (42%), Gaps = 7/128 (5%)
Frame = +1
Query: 52 TSTAAPDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGKTYRHEC 210
T A PD C + CK G++C+A K C C D CP +Y R D KTY C
Sbjct: 437 TGKALPDLCRNFHCKRGKVCQADKQGKPSCICQDPAACPST-KDYKRVCGTDNKTYDGTC 495
Query: 211 DLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAM 390
L+ KC + G++ G + + Y C+ + D+ E F R+
Sbjct: 496 QLFGTKC-------QLEGTKMGRQ-----LHLDYMGACKHIPHCTDY--EVNQFPLRMRD 541
Query: 391 WFRDLYSQ 414
W +++ Q
Sbjct: 542 WLKNILMQ 549
>sp|Q14515|SPRL1_HUMAN SPARC-like protein 1 precursor (High endothelial venule protein)
(Hevin) (MAST 9)
Length = 664
Score = 48.1 bits (113), Expect = 9e-06
Identities = 35/129 (27%), Positives = 53/129 (41%), Gaps = 6/129 (4%)
Frame = +1
Query: 64 APDQCASVQCKDGELCEAGK-----CKCMDQCPEEWNNYPRQLC-VDGKTYRHECDLWRN 225
A D C S QCK G +C+A + C C D Q+C D +TY C L+
Sbjct: 429 AVDSCMSFQCKRGHICKADQQGKPHCVCQDPVTCPPTKPLDQVCGTDNQTYASSCHLFAT 488
Query: 226 KCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAMWFRDL 405
KC R G++ G + + YF C+ + D+ E F R+ W +++
Sbjct: 489 KC-------RLEGTKKGHQ-----LQLDYFGACKSIPTCTDF--EVIQFPLRMRDWLKNI 534
Query: 406 YSQKWSVAS 432
Q + S
Sbjct: 535 LMQLYEANS 543
>sp|P70663|SPRL1_MOUSE SPARC-like protein 1 precursor (Matrix glycoprotein Sc1)
(Extracellular matrix protein 2)
Length = 650
Score = 46.2 bits (108), Expect = 4e-05
Identities = 35/119 (29%), Positives = 52/119 (43%), Gaps = 6/119 (5%)
Frame = +1
Query: 76 CASVQCKDGELCEA---GKCKCMDQCPEEW--NNYPRQLC-VDGKTYRHECDLWRNKCYC 237
C + QCK G +C+ GK C+ Q PE Q C D +TY C L+ KC
Sbjct: 419 CTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKC-- 476
Query: 238 AKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAMWFRDLYSQ 414
R G++ G + + YF C+ + A D+ E A F R+ W +++ Q
Sbjct: 477 -----RLEGTKKGHQ-----LQLDYFGACKSIPACTDF--EVAQFPLRMRDWLKNILMQ 523
>sp|P24054|SPRL1_RAT SPARC-like protein 1 precursor (Matrix glycoprotein Sc1)
Length = 634
Score = 45.1 bits (105), Expect = 8e-05
Identities = 34/121 (28%), Positives = 49/121 (40%), Gaps = 6/121 (4%)
Frame = +1
Query: 70 DQCASVQCKDGELCEA---GKCKCMDQCPEEW--NNYPRQLC-VDGKTYRHECDLWRNKC 231
D C + QCK G C+ GK C+ Q PE Q C D +TY C L+ KC
Sbjct: 401 DSCMNFQCKRGHTCKTDQHGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKC 460
Query: 232 YCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAMWFRDLYS 411
+ K + + YF C+ + A D+ E A F R+ W +++
Sbjct: 461 MLEGT------------KKGHQLQLDYFGACKSIPACTDF--EVAQFPLRMRDWLKNILM 506
Query: 412 Q 414
Q
Sbjct: 507 Q 507
>sp|P16975|SPRC_RAT SPARC precursor (Secreted protein acidic and rich in cysteine)
(Osteonectin) (ON) (Basement-membrane protein 40)
(BM-40)
Length = 302
Score = 41.6 bits (96), Expect = 9e-04
Identities = 32/130 (24%), Positives = 54/130 (41%), Gaps = 7/130 (5%)
Frame = +1
Query: 37 QKVLETSTAAPDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGKT 195
++ +E A + C + CK G++CE + C C D CP + + D KT
Sbjct: 58 EETVEEVVACNNPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNKT 117
Query: 196 YRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFH 375
+ C + KC G++ G HK + + Y C+ +A D SE F
Sbjct: 118 FDSSCHFFATKC-------TLEGTKKG-HK----LHLDYIGPCKYIAPCLD--SELTEFP 163
Query: 376 ARLAMWFRDL 405
R+ W +++
Sbjct: 164 LRMRDWLKNV 173
>sp|P09486|SPRC_HUMAN SPARC precursor (Secreted protein acidic and rich in cysteine)
(Osteonectin) (ON) (Basement-membrane protein 40)
(BM-40)
Length = 303
Score = 40.8 bits (94), Expect = 0.001
Identities = 35/150 (23%), Positives = 60/150 (40%), Gaps = 7/150 (4%)
Frame = +1
Query: 34 GQKVLETSTAAPDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGK 192
G + E A + C + CK G++CE + C C D CP + + D K
Sbjct: 58 GAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNK 117
Query: 193 TYRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASF 372
T+ C + KC G++ G HK + + Y C+ + D SE F
Sbjct: 118 TFDSSCHFFATKC-------TLEGTKKG-HK----LHLDYIGPCKYIPPCLD--SELTEF 163
Query: 373 HARLAMWFRDLYSQKWSVASGKSDDQSLLS 462
R+ W +++ + + +D +LL+
Sbjct: 164 PLRMRDWLKNVLVTLYE----RDEDNNLLT 189
>sp|P13213|SPRC_BOVIN SPARC precursor (Secreted protein acidic and rich in cysteine)
(Osteonectin) (ON) (Basement-membrane protein 40)
(BM-40)
Length = 304
Score = 40.8 bits (94), Expect = 0.001
Identities = 35/150 (23%), Positives = 60/150 (40%), Gaps = 7/150 (4%)
Frame = +1
Query: 34 GQKVLETSTAAPDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGK 192
G + E A + C + CK G++CE + C C D CP + + D K
Sbjct: 58 GAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNK 117
Query: 193 TYRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASF 372
T+ C + KC G++ G HK + + Y C+ + D SE F
Sbjct: 118 TFDSSCHFFATKC-------TLEGTKKG-HK----LHLDYIGPCKYIPPCLD--SELTEF 163
Query: 373 HARLAMWFRDLYSQKWSVASGKSDDQSLLS 462
R+ W +++ + + +D +LL+
Sbjct: 164 PLRMRDWLKNVLVTLYE----RDEDNNLLT 189
>sp|P36378|SPRC_XENLA SPARC precursor (Secreted protein acidic and rich in cysteine)
(Osteonectin) (ON) (Basement-membrane protein 40)
(BM-40)
Length = 300
Score = 40.8 bits (94), Expect = 0.001
Identities = 31/126 (24%), Positives = 52/126 (41%), Gaps = 7/126 (5%)
Frame = +1
Query: 49 ETSTAAPDQCASVQCKDGELCEAGK-----CKCMDQ--CPEEWNNYPRQLCVDGKTYRHE 207
E + + C + CK G++CE + C C D CP + + D KTY
Sbjct: 60 EEEEPSENPCLNHHCKHGKVCEVDESNTPLCVCQDPSTCPTSVGEFEKICGTDNKTYDSS 119
Query: 208 CDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLA 387
C + KC G++ G HK + + Y C+ +A D +E + F R+
Sbjct: 120 CHFFATKC-------TLEGTKKG-HK----LHLDYIGPCKYIAPCLD--NELSEFPLRIG 165
Query: 388 MWFRDL 405
W +++
Sbjct: 166 DWLKNV 171
>sp|P07214|SPRC_MOUSE SPARC precursor (Secreted protein acidic and rich in cysteine)
(Osteonectin) (ON) (Basement-membrane protein 40)
(BM-40)
Length = 302
Score = 39.7 bits (91), Expect = 0.003
Identities = 31/121 (25%), Positives = 50/121 (41%), Gaps = 7/121 (5%)
Frame = +1
Query: 64 APDQCASVQCKDGELCEAGK-----CKCMD--QCPEEWNNYPRQLCVDGKTYRHECDLWR 222
A + C + CK G++CE + C C D CP + + D KT+ C +
Sbjct: 67 ADNPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFA 126
Query: 223 NKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEEASFHARLAMWFRD 402
KC G++ G HK + + Y C+ +A D SE F R+ W ++
Sbjct: 127 TKC-------TLEGTKKG-HK----LHLDYIGPCKYIAPCLD--SELTEFPLRMRDWLKN 172
Query: 403 L 405
+
Sbjct: 173 V 173
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,807,660
Number of Sequences: 369166
Number of extensions: 1140661
Number of successful extensions: 3667
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3337
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3626
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2584230740
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)