Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_010_P11
(725 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P34714|SPRC_CAEEL SPARC precursor (Secreted protein acid... 44 6e-04
sp|P70663|SPRL1_MOUSE SPARC-like protein 1 precursor (Matri... 39 0.011
sp|Q14515|SPRL1_HUMAN SPARC-like protein 1 precursor (High ... 39 0.019
sp|P24054|SPRL1_RAT SPARC-like protein 1 precursor (Matrix ... 37 0.041
sp|P42322|CANB1_NAEGR Calcineurin B subunit (Protein phosph... 30 5.0
sp|P87072|CANB_NEUCR Calcineurin B subunit (Protein phospha... 30 6.6
sp|P36378|SPRC_XENLA SPARC precursor (Secreted protein acid... 30 8.6
>sp|P34714|SPRC_CAEEL SPARC precursor (Secreted protein acidic and rich in cysteine)
(Osteonectin) (ON) (Basement-membrane protein 40)
(BM-40)
Length = 264
Score = 43.5 bits (101), Expect = 6e-04
Identities = 42/199 (21%), Positives = 73/199 (36%), Gaps = 16/199 (8%)
Frame = +2
Query: 35 DGKTYRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEE 214
+ +T+ CDL+R +C C + C S + ++Y C+ L D +EE
Sbjct: 95 NNQTFTSLCDLYRERCLCKRKSKEC------SKAFNAKVHLEYLGECKKL----DECTEE 144
Query: 215 --ASFHARLAMWF----------RDLYSQKWSVASGKXXXXXXXXXXXXKTRSSATTLFN 358
A F R+A W R+L+ +W L +
Sbjct: 145 HMAQFPERMADWLFQVMKELKKRRELHKLEWE------------------------ELLS 180
Query: 359 AQTSSPYGVHGGYLSFWFCEMD-KNNNGNLDRSEIALLSQLLNPSTPCLNTFLSKC---G 526
+ H + + FCE+D K ++ ++ E+ ++ + P C+ FL C
Sbjct: 181 EAENDDEKKHVYPVIWKFCELDTKPHDKSVSHHELIPITAPVIPMESCIKPFLEGCDANN 240
Query: 527 SGTISFSAWNSCFKVPKAE 583
G IS W C + + E
Sbjct: 241 DGNISIKEWGKCLGLKEGE 259
>sp|P70663|SPRL1_MOUSE SPARC-like protein 1 precursor (Matrix glycoprotein Sc1)
(Extracellular matrix protein 2)
Length = 650
Score = 39.3 bits (90), Expect = 0.011
Identities = 47/208 (22%), Positives = 74/208 (35%), Gaps = 21/208 (10%)
Frame = +2
Query: 23 QLC-VDGKTYRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCD 199
Q C D +TY C L+ KC R G++ G + + YF C+ + A D
Sbjct: 456 QACGTDNQTYASSCHLFATKC-------RLEGTKKGHQ-----LQLDYFGACKSIPACTD 503
Query: 200 WPSEEASFHARLAMWFRDLYSQKWSVASGKXXXXXXXXXXXXKTRSSATTLFNAQTSSPY 379
+ E A F R+ W +++ Q + K RS ++ +
Sbjct: 504 F--EVAQFPLRMRDWLKNILMQLYE------PNPKHGGYLNEKQRSKVKKIYLDEKRLLA 555
Query: 380 GVHG---------------GYLSFW-FCEMDKNNNGN-LDRSEIALLSQLLNPSTPCLNT 508
G H Y W F E+D++ L SE+A L L P C+
Sbjct: 556 GDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITR 615
Query: 509 FLSKCGSGT---ISFSAWNSCFKVPKAE 583
F +C I+ W CF + + +
Sbjct: 616 FFEECDPNKDKHITLKEWGHCFGIKEED 643
>sp|Q14515|SPRL1_HUMAN SPARC-like protein 1 precursor (High endothelial venule protein)
(Hevin) (MAST 9)
Length = 664
Score = 38.5 bits (88), Expect = 0.019
Identities = 42/202 (20%), Positives = 71/202 (35%), Gaps = 15/202 (7%)
Frame = +2
Query: 23 QLC-VDGKTYRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCD 199
Q+C D +TY C L+ KC R G++ G + + YF C+ + D
Sbjct: 470 QVCGTDNQTYASSCHLFATKC-------RLEGTKKGHQ-----LQLDYFGACKSIPTCTD 517
Query: 200 WPSEEASFHARLAMWFRDLYSQKWSVASGKXXXXXXXXXXXXK----------TRSSATT 349
+ E F R+ W +++ Q + S K
Sbjct: 518 F--EVIQFPLRMRDWLKNILMQLYEANSEHAGYLNEKQRNKVKKIYLDEKRLLAGDHPID 575
Query: 350 LFNAQTSSPYGVHGGYLSFWFCEMDKNNNGN-LDRSEIALLSQLLNPSTPCLNTFLSKCG 526
L Y ++ + + F E+D++ L SE+A L L P C+ F +C
Sbjct: 576 LLLRDFKKNYHMYVYPVHWQFSELDQHPMDRVLTHSELAPLRASLVPMEHCITRFFEECD 635
Query: 527 SGT---ISFSAWNSCFKVPKAE 583
I+ W CF + + +
Sbjct: 636 PNKDKHITLKEWGHCFGIKEED 657
>sp|P24054|SPRL1_RAT SPARC-like protein 1 precursor (Matrix glycoprotein Sc1)
Length = 634
Score = 37.4 bits (85), Expect = 0.041
Identities = 45/208 (21%), Positives = 71/208 (34%), Gaps = 21/208 (10%)
Frame = +2
Query: 23 QLC-VDGKTYRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCD 199
Q C D +TY C L+ KC + K + + YF C+ + A D
Sbjct: 440 QACGTDNQTYASSCHLFATKCMLEGT------------KKGHQLQLDYFGACKSIPACTD 487
Query: 200 WPSEEASFHARLAMWFRDLYSQKWSVASGKXXXXXXXXXXXXKTRSSATTLFNAQTSSPY 379
+ E A F R+ W +++ Q + K RS ++ +
Sbjct: 488 F--EVAQFPLRMRDWLKNILMQLYE------PNPKHGGYLNEKQRSKVKKIYLDEKRLLA 539
Query: 380 GVHG---------------GYLSFW-FCEMDKNNNGN-LDRSEIALLSQLLNPSTPCLNT 508
G H Y W F E+D++ L SE+A L L P C+
Sbjct: 540 GDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITR 599
Query: 509 FLSKCGSGT---ISFSAWNSCFKVPKAE 583
F +C I+ W CF + + +
Sbjct: 600 FFEECDPNKDKHITLKEWGHCFGIKEED 627
>sp|P42322|CANB1_NAEGR Calcineurin B subunit (Protein phosphatase 2B regulatory subunit)
(Calcineurin regulatory subunit)
Length = 177
Score = 30.4 bits (67), Expect = 5.0
Identities = 14/59 (23%), Positives = 32/59 (54%), Gaps = 1/59 (1%)
Frame = +2
Query: 410 FCEMDKNNNGNLDRSEIALLSQL-LNPSTPCLNTFLSKCGSGTISFSAWNSCFKVPKAE 583
F ++DK+ NG + + E ++ +L +NP + + + G G+++F + + V A+
Sbjct: 34 FKKLDKDGNGTISKDEFLMIPELAVNPLVKRVISIFDENGDGSVNFKEFIAALSVFNAQ 92
>sp|P87072|CANB_NEUCR Calcineurin B subunit (Protein phosphatase 2B regulatory subunit)
(Calcineurin regulatory subunit)
Length = 174
Score = 30.0 bits (66), Expect = 6.6
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +2
Query: 410 FCEMDKNNNGNLDRSEIALLSQL-LNPSTPCLNTFLSKCGSGTISFSAWNS 559
F ++DK+N+G ++R E L Q+ NP + + G G + F + S
Sbjct: 30 FMKLDKDNSGTIEREEFLSLPQISTNPLATRMIAIFDEDGGGDVDFQEFVS 80
>sp|P36378|SPRC_XENLA SPARC precursor (Secreted protein acidic and rich in cysteine)
(Osteonectin) (ON) (Basement-membrane protein 40)
(BM-40)
Length = 300
Score = 29.6 bits (65), Expect = 8.6
Identities = 38/196 (19%), Positives = 72/196 (36%), Gaps = 13/196 (6%)
Frame = +2
Query: 35 DGKTYRHECDLWRNKCYCAKSDSRCGGSEFGSHKPTDTIVIQYFDGCRDLAAQCDWPSEE 214
D KTY C + KC G++ G HK + + Y C+ +A D +E
Sbjct: 112 DNKTYDSSCHFFATKC-------TLEGTKKG-HK----LHLDYIGPCKYIAPCLD--NEL 157
Query: 215 ASFHARLAMWFRDLYSQKWSVASGKXXXXXXXXXXXXKTRSSATTLFNAQTSSP------ 376
+ F R+ W +++ + K + L + + S
Sbjct: 158 SEFPLRIGDWLKNVLVSLYERDENNNLLNEKQKLRVKKIHENEKRLESWRPHSELLVRDF 217
Query: 377 ---YGVHGGYLSFWFCEMDKNN-NGNLDRSEIALLSQLLNPSTPCLNTFLSKC---GSGT 535
Y ++ + + F ++D++ +G L +E++ L L P C F +C
Sbjct: 218 EKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELSPLRAPLIPMEHCTTRFFDECDIDDDKY 277
Query: 536 ISFSAWNSCFKVPKAE 583
I+ W CF + + +
Sbjct: 278 IALEEWAKCFGIKEQD 293
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,899,281
Number of Sequences: 369166
Number of extensions: 1598670
Number of successful extensions: 3162
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 3078
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3162
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6510836890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)