Planaria EST Database

DrC_00604

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00604
         (951 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O75494|FUSIP_HUMAN  FUS-interacting serine-arginine-rich ...    81   4e-15
sp|Q8WXF0|SRR35_HUMAN  35 kDa SR repressor protein (SRrp35)        80   6e-15
sp|P27476|NSR1_YEAST  Nuclear localization sequence binding ...    57   1e-07
sp|P30352|SFRS2_CHICK  Splicing factor, arginine/serine-rich...    57   1e-07
sp|Q6PDU1|SFRS2_RAT  Splicing factor, arginine/serine-rich 2...    57   1e-07
sp|Q5R1W5|SFRS2_PANTR  Splicing factor, arginine/serine-rich...    57   1e-07
sp|Q09511|RSP4_CAEEL  Probable splicing factor, arginine/ser...    55   2e-07
sp|O22315|SFRS1_ARATH  Pre-mRNA splicing factor SF2 (SR1 pro...    53   1e-06
sp|P62997|TRA2B_RAT  Arginine/serine-rich splicing factor 10...    51   5e-06
sp|Q9NEW6|RSP3_CAEEL  Probable splicing factor, arginine/ser...    50   7e-06

>sp|O75494|FUSIP_HUMAN FUS-interacting serine-arginine-rich protein 1 (TLS-associated
           protein with Ser-Arg repeats) (TLS-associated protein
           with SR repeats) (TASR) (TLS-associated serine-arginine
           protein) (TLS-associated SR protein) (40 kDa
           SR-repressor protein) (SRrp40) (Splicing factor SRp38)
 sp|Q9R0U0|FUSIP_MOUSE FUS-interacting serine-arginine-rich protein 1 (TLS-associated
           protein with Ser-Arg repeats) (TLS-associated protein
           with SR repeats) (TASR) (TLS-associated serine-arginine
           protein) (TLS-associated SR protein) (Neural-specific SR
           protein) (Neural-salient serine/arginine-rich protein)
          Length = 262

 Score = 81.3 bits (199), Expect = 4e-15
 Identities = 38/84 (45%), Positives = 59/84 (70%)
 Frame = +1

Query: 331 RRDELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKRSL 510
           R ++L+R FGRYG IV   +P+D+Y+  P+GFA+VQFED+RDAE++   L    I  R +
Sbjct: 22  RSEDLRREFGRYGPIVDVYVPLDFYTRRPRGFAYVQFEDVRDAEDALHNLDRKWICGRQI 81

Query: 511 RLEFATGTKKTPDEMRTVTRRGRS 582
            ++FA G +KTP++M+   + GR+
Sbjct: 82  EIQFAQGDRKTPNQMK--AKEGRN 103

>sp|Q8WXF0|SRR35_HUMAN 35 kDa SR repressor protein (SRrp35)
          Length = 261

 Score = 80.5 bits (197), Expect = 6e-15
 Identities = 38/86 (44%), Positives = 58/86 (67%)
 Frame = +1

Query: 331 RRDELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKRSL 510
           R ++L+R FGRYG IV   +P+D+Y+  P+GFA+VQFED+RDAE++   L    +  R +
Sbjct: 22  RPEDLRREFGRYGPIVDVYIPLDFYTRRPRGFAYVQFEDVRDAEDALYNLNRKWVCGRQI 81

Query: 511 RLEFATGTKKTPDEMRTVTRRGRSNS 588
            ++FA G +KTP +M++  R   S S
Sbjct: 82  EIQFAQGDRKTPGQMKSKERHPCSPS 107

>sp|P27476|NSR1_YEAST Nuclear localization sequence binding protein (P67)
          Length = 414

 Score = 56.6 bits (135), Expect = 1e-07
 Identities = 25/68 (36%), Positives = 43/68 (63%)
 Frame = +1

Query: 325 NDRRDELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKR 504
           N  RD +  +F ++G +V+  +P    +  PKGF +VQF ++ DA+++ D LQG  I  R
Sbjct: 277 NADRDAIFELFAKHGEVVSVRIPTHPETEQPKGFGYVQFSNMEDAKKALDALQGEYIDNR 336

Query: 505 SLRLEFAT 528
            +RL+F++
Sbjct: 337 PVRLDFSS 344


 Score = 33.5 bits (75), Expect = 0.91
 Identities = 16/62 (25%), Positives = 32/62 (51%)
 Frame = +1

Query: 343 LKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKRSLRLEF 522
           LK+ F   G ++ A +  +  +   +G+ +V FE+   AE++   +QG  I  R +  + 
Sbjct: 184 LKKEFEHIGGVIGARVIYERGTDRSRGYGYVDFENKSYAEKAIQEMQGKEIDGRPINCDM 243

Query: 523 AT 528
           +T
Sbjct: 244 ST 245

>sp|P30352|SFRS2_CHICK Splicing factor, arginine/serine-rich 2 (Splicing factor SC35)
           (SC-35) (Splicing component, 35 kDa) (PR264 protein)
          Length = 221

 Score = 56.6 bits (135), Expect = 1e-07
 Identities = 26/63 (41%), Positives = 40/63 (63%)
 Frame = +1

Query: 337 DELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKRSLRL 516
           D L+R+F +YG +    +P D Y+   +GFAFV+F D RDAE++ D + G  +  R LR+
Sbjct: 28  DTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRV 87

Query: 517 EFA 525
           + A
Sbjct: 88  QMA 90

>sp|Q6PDU1|SFRS2_RAT Splicing factor, arginine/serine-rich 2 (Splicing factor SC35)
           (SC-35) (Splicing component, 35 kDa)
 sp|Q62093|SFRS2_MOUSE Splicing factor, arginine/serine-rich 2 (Splicing factor SC35)
           (SC-35) (Splicing component, 35 kDa) (PR264 protein)
 sp|Q01130|SFRS2_HUMAN Splicing factor, arginine/serine-rich 2 (Splicing factor SC35)
           (SC-35) (Splicing component, 35 kDa) (PR264 protein)
          Length = 221

 Score = 56.6 bits (135), Expect = 1e-07
 Identities = 26/63 (41%), Positives = 40/63 (63%)
 Frame = +1

Query: 337 DELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKRSLRL 516
           D L+R+F +YG +    +P D Y+   +GFAFV+F D RDAE++ D + G  +  R LR+
Sbjct: 28  DTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRV 87

Query: 517 EFA 525
           + A
Sbjct: 88  QMA 90

>sp|Q5R1W5|SFRS2_PANTR Splicing factor, arginine/serine-rich 2 (Splicing factor SC35)
           (SC-35) (Splicing component, 35 kDa)
          Length = 221

 Score = 56.6 bits (135), Expect = 1e-07
 Identities = 26/63 (41%), Positives = 40/63 (63%)
 Frame = +1

Query: 337 DELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKRSLRL 516
           D L+R+F +YG +    +P D Y+   +GFAFV+F D RDAE++ D + G  +  R LR+
Sbjct: 28  DTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRV 87

Query: 517 EFA 525
           + A
Sbjct: 88  QMA 90

>sp|Q09511|RSP4_CAEEL Probable splicing factor, arginine/serine-rich 4 (RNA-binding
           protein srp-2) (CeSC35)
          Length = 196

 Score = 55.5 bits (132), Expect = 2e-07
 Identities = 34/94 (36%), Positives = 49/94 (52%), Gaps = 9/94 (9%)
 Frame = +1

Query: 337 DELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKRSLRL 516
           ++L+R F RYG+I    +P D YS   KGF FV+F + RDAE + DR  G  +  R LR+
Sbjct: 33  NDLRRTFERYGDIGDVHIPRDKYSRQSKGFGFVRFYERRDAEHALDRTDGKLVDGRELRV 92

Query: 517 EFATGTKKTPD---------EMRTVTRRGRSNSP 591
             A   + + +           R+ + R RS SP
Sbjct: 93  TLAKYDRPSDERGGRGGGGGRRRSRSPRRRSRSP 126

>sp|O22315|SFRS1_ARATH Pre-mRNA splicing factor SF2 (SR1 protein)
          Length = 303

 Score = 53.1 bits (126), Expect = 1e-06
 Identities = 28/76 (36%), Positives = 43/76 (56%)
 Frame = +1

Query: 331 RRDELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKRSL 510
           R  E++ +F +YG +V   L V      P G+AFV+F+D RDAE++     GY      L
Sbjct: 19  REREVEDLFSKYGPVVQIDLKVPPR---PPGYAFVEFDDARDAEDAIHGRDGYDFDGHRL 75

Query: 511 RLEFATGTKKTPDEMR 558
           R+E A G +++ D+ R
Sbjct: 76  RVELAHGGRRSSDDTR 91

>sp|P62997|TRA2B_RAT Arginine/serine-rich splicing factor 10 (Transformer-2-beta)
           (HTRA2-beta) (Transformer 2 protein homolog) (RA301)
 sp|P62996|TRA2B_MOUSE Arginine/serine-rich splicing factor 10 (Transformer-2-beta)
           (HTRA2-beta) (Transformer 2 protein homolog)
           (Silica-induced gene 41 protein) (SIG-41)
 sp|P62995|TRA2B_HUMAN Arginine/serine-rich splicing factor 10 (Transformer-2-beta)
           (HTRA2-beta) (Transformer 2 protein homolog)
          Length = 288

 Score = 50.8 bits (120), Expect = 5e-06
 Identities = 25/70 (35%), Positives = 43/70 (61%)
 Frame = +1

Query: 316 LY*NDRRDELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRI 495
           LY  +R  +L+ +F +YG I   ++  D  S   +GFAFV FE++ DA+E+ +R  G  +
Sbjct: 127 LYTTER--DLREVFSKYGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMEL 184

Query: 496 GKRSLRLEFA 525
             R +R++F+
Sbjct: 185 DGRRIRVDFS 194

>sp|Q9NEW6|RSP3_CAEEL Probable splicing factor, arginine/serine-rich 3 (CeSF2)
           (CeSF2/ASF)
          Length = 258

 Score = 50.4 bits (119), Expect = 7e-06
 Identities = 29/67 (43%), Positives = 37/67 (55%)
 Frame = +1

Query: 331 RRDELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRIGKRSL 510
           R  E++ IF +YG I      VD  SG    FAFV+FED RDAE++     GY    R +
Sbjct: 21  REKEVEDIFHKYGRIKY----VDIKSGRGPAFAFVEFEDHRDAEDAVRARDGYEFDGRRI 76

Query: 511 RLEFATG 531
           R+EF  G
Sbjct: 77  RVEFTRG 83

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,756,269
Number of Sequences: 369166
Number of extensions: 1385470
Number of successful extensions: 3519
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3354
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3507
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9895030780
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00604

  1. Dr_sW_018_N14
  2. Dr_sW_015_N18
  3. Dr_sW_025_P22
  4. Dr_sW_026_O04