Planaria EST Database


DrC_00581

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00581
         (1093 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P25235|RIB2_RAT  Dolichyl-diphosphooligosaccharide--prote...   183   7e-46
sp|Q9DBG6|RPN2_MOUSE  Dolichyl-diphosphooligosaccharide--pro...   182   1e-45
sp|P04844|RIB2_HUMAN  Dolichyl-diphosphooligosaccharide--pro...   182   1e-45
sp|Q6BS08|ATG1_DEBHA  Serine/threonine-protein kinase ATG1 (...    41   0.005
sp|Q02795|OSTD_YEAST  Dolichyl-diphosphooligosaccharide--pro...    35   0.38 
sp|O68433|LEP4_LEGPN  Type 4 prepilin-like proteins leader p...    35   0.38 
sp|P50976|PTLCB_STRMU  PTS system lactose-specific EIICB com...    32   4.2  
sp|Q19537|RFA1_CAEEL  Probable replication factor A 73 kDa s...    32   4.2  
sp|P41003|SMC2_SCHPO  Structural maintenance of chromosome 2...    31   7.1  
sp|O67151|GCSH1_AQUAE  Glycine cleavage system H protein 1         30   9.3  
>sp|P25235|RIB2_RAT Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
            subunit precursor (Ribophorin II) (RPN-II)
          Length = 631

 Score =  183 bits (465), Expect = 7e-46
 Identities = 121/351 (34%), Positives = 186/351 (52%), Gaps = 2/351 (0%)
 Frame = +2

Query: 2    AALVAETVKNVQSATTVLSRKSLVFDRTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETR 181
            AA+  E  K+V S  TVL +  + F     ++EL+  +V+  L  G+Y   + ++  + R
Sbjct: 303  AAVKLEHAKSVASRATVLQK--MPFSLVGDVFELNFKNVK--LPSGYYDFSVRVE-GDNR 357

Query: 182  YNEAVGVALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDM 361
            Y  A  V L VK+  +V I NV+LS  ++DQ       ++T  +        + H    +
Sbjct: 358  YI-ANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL 416

Query: 362  KFVIRDHSQSVK--PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLS 535
             F + D +   +  PHQ FV+L +  T QE++FV E D  +VYKF LD  +   +FD+ S
Sbjct: 417  FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSAS 476

Query: 536  GDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSG 715
            G Y + L++GD+ ++ P  W  A++ +  P         E+  TV    +++  P     
Sbjct: 477  GTYTLYLIIGDATLKNPILWNVADVVIKFPEE-------EAPSTVLS--QNLFTP----- 522

Query: 716  PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPI 895
                K +I H+F+  EKR   ++S  FT L I+ P +L+ ALW+ +G NVSNF+   S +
Sbjct: 523  ----KQEIQHLFREPEKRPPTVVSNTFTAL-ILSPLLLLFALWIRIGANVSNFTFAPSTV 577

Query: 896  MFHVGIIAIFGLYYCYWGYLNMFTTLQCLLPLGMFTAFFGNRVLRQIASKR 1048
            +FH+G  A+ GL Y YW  LNMF TL+ L  LG  T   GNR+L Q A KR
Sbjct: 578  IFHLGHAAMLGLMYVYWTQLNMFQTLKYLAVLGTVTFLAGNRMLAQQAVKR 628
>sp|Q9DBG6|RPN2_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
            subunit precursor (Ribophorin II) (RPN-II)
          Length = 631

 Score =  182 bits (463), Expect = 1e-45
 Identities = 120/351 (34%), Positives = 186/351 (52%), Gaps = 2/351 (0%)
 Frame = +2

Query: 2    AALVAETVKNVQSATTVLSRKSLVFDRTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETR 181
            AA+  E  K+  +  TVL +    F     ++EL+  +V+  L+ G+Y   + ++  ++R
Sbjct: 303  AAVKLEHAKSAATRATVLQKTP--FSLVGNVFELNFKNVK--LSSGYYDFSVRVE-GDSR 357

Query: 182  YNEAVGVALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDM 361
            Y  A  V L VK+  +V I NV+LS  ++DQ       ++T  +        + H    +
Sbjct: 358  YI-ANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL 416

Query: 362  KFVIRDHSQSVK--PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLS 535
             F + D +   +  PHQ FV+L +  T QE++FV E D  +VYKF LD  +   +FD+ S
Sbjct: 417  FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSAS 476

Query: 536  GDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSG 715
            G Y + L++GD+ ++ P  W  A++ +  P         E+  TV    +S+  P     
Sbjct: 477  GTYTLYLIIGDATLKNPILWNVADVVIKFPEE-------EAPSTVLS--QSLFTP----- 522

Query: 716  PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPI 895
                K +I H+F+  EKR   ++S  FT L I+ P +L+ ALW+ +G NVSNF+   S +
Sbjct: 523  ----KQEIQHLFREPEKRPPTVVSNTFTAL-ILSPLLLLFALWIRIGANVSNFTFAPSTV 577

Query: 896  MFHVGIIAIFGLYYCYWGYLNMFTTLQCLLPLGMFTAFFGNRVLRQIASKR 1048
            +FH+G  A+ GL Y YW  LNMF TL+ L  LG  T   GNR+L Q A KR
Sbjct: 578  IFHLGHAAMLGLMYIYWTQLNMFQTLKYLAVLGTVTFLAGNRMLAQHAVKR 628
>sp|P04844|RIB2_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
            subunit precursor (Ribophorin II) (RPN-II) (RIBIIR)
          Length = 631

 Score =  182 bits (463), Expect = 1e-45
 Identities = 121/351 (34%), Positives = 186/351 (52%), Gaps = 2/351 (0%)
 Frame = +2

Query: 2    AALVAETVKNVQSATTVLSRKSLVFDRTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETR 181
            A +  E  K+V S  TVL + S  F     ++EL+  +V+   + G+Y   ++++  + R
Sbjct: 303  ATVKLEHAKSVASRATVLQKTS--FTPVGDVFELNFMNVK--FSSGYYDFLVEVE-GDNR 357

Query: 182  YNEAVGVALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDM 361
            Y  A  V L VK+  +V I NV+LS  ++DQ       ++T  +        + H    +
Sbjct: 358  YI-ANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL 416

Query: 362  KFVIRDHSQSVK--PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLS 535
             F + D +   +  PHQ FV+L +  T QE++FV E D  +VYKF LD  +   +FD+ S
Sbjct: 417  FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSAS 476

Query: 536  GDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSG 715
            G Y + L++GD+ ++ P  W  A++ +  P         E+  TV    +++  P     
Sbjct: 477  GTYTLYLIIGDATLKNPILWNVADVVIKFPEE-------EAPSTVLS--QNLFTP----- 522

Query: 716  PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPI 895
                K +I H+F+  EKR   ++S  FT L I+ P +L+ ALW+ +G NVSNF+   S I
Sbjct: 523  ----KQEIQHLFREPEKRPPTVVSNTFTAL-ILSPLLLLFALWIRIGANVSNFTFAPSTI 577

Query: 896  MFHVGIIAIFGLYYCYWGYLNMFTTLQCLLPLGMFTAFFGNRVLRQIASKR 1048
            +FH+G  A+ GL Y YW  LNMF TL+ L  LG  T   GNR+L Q A KR
Sbjct: 578  IFHLGHAAMLGLMYVYWTQLNMFQTLKYLAILGSVTFLAGNRMLAQQAVKR 628
>sp|Q6BS08|ATG1_DEBHA Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1)
          Length = 875

 Score = 41.2 bits (95), Expect = 0.005
 Identities = 36/123 (29%), Positives = 59/123 (47%), Gaps = 9/123 (7%)
 Frame = +2

Query: 80  RTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETRYNEAVGVALVV--KVICQVSIENVEL 253
           +T G+Y     S+  ++ KG ++     K   T+ NE+V +  VV  K+  +  +EN+E+
Sbjct: 17  KTIGVY-----SIGPEIGKGSFATVY--KCFNTKTNESVAIKSVVRSKLKSKKLVENLEI 69

Query: 254 SIK-----ERDQITGILNYKLTMG--SLLSDKLSMNWHHRLDMKFVIRDHSQSVKPHQVF 412
            I      +   I G+L+YK T     L+ D  SM      D+ + IR  +Q +K H V 
Sbjct: 70  EISILKTMKHPHIVGLLDYKQTTSHFHLVMDYCSMG-----DLSYFIRKRNQLIKTHPVI 124

Query: 413 VKL 421
             L
Sbjct: 125 SSL 127
>sp|Q02795|OSTD_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase
           delta subunit precursor (Oligosaccharyl transferase
           delta subunit)
          Length = 286

 Score = 35.0 bits (79), Expect = 0.38
 Identities = 24/93 (25%), Positives = 43/93 (46%), Gaps = 3/93 (3%)
 Frame = +2

Query: 725 VKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMFH 904
           +KP+I H+F    KR  + I+ +F ++  +    L+V          +N   G++ + F 
Sbjct: 176 IKPEIHHIFHAEPKRVAKPIAVIFVLIIFITILSLIVTWLNSCAAAFNNIPTGVTAVYFL 235

Query: 905 VGIIAIFG---LYYCYWGYLNMFTTLQCLLPLG 994
             I  I G   ++  Y+   ++F TL   L LG
Sbjct: 236 GFIATIVGFEVIFARYYLGTSIFETLFSSLYLG 268
>sp|O68433|LEP4_LEGPN Type 4 prepilin-like proteins leader peptide processing enzyme
            [Includes: Leader peptidase (Prepilin peptidase);
            N-methyltransferase ]
          Length = 287

 Score = 35.0 bits (79), Expect = 0.38
 Identities = 24/84 (28%), Positives = 40/84 (47%), Gaps = 7/84 (8%)
 Frame = +2

Query: 779  LISTVFTILC--IVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGY 952
            LIS VF  L   ++P  + +  LW+ +  N  N  V L      V +++  G Y   W +
Sbjct: 141  LISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNVFVSLD-----VAVLSCAGAYLALWLF 195

Query: 953  LNMFTTLQCLLPLG-----MFTAF 1009
            +N+F  + C + +G     +F AF
Sbjct: 196  INLFYLMTCKVCMGHGDFKLFAAF 219
>sp|P50976|PTLCB_STRMU PTS system lactose-specific EIICB component (EIICB-Lac) (EII-Lac)
           [Includes: Lactose permease IIC component (PTS system
           lactose-specific EIIC component); Lactose-specific
           phosphotransferase enzyme IIB component (PTS system
           lactose-specific EIIB component)]
          Length = 568

 Score = 31.6 bits (70), Expect = 4.2
 Identities = 24/83 (28%), Positives = 42/83 (50%), Gaps = 6/83 (7%)
 Frame = +2

Query: 716 PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVG--LS 889
           P  V P I  +FK        +    F+I+ +   + + +A+  ++GVNV+  S+G  L+
Sbjct: 166 PEEVPPNISQVFK-------DIFPFAFSIIIL---YAIQLAIKAVIGVNVAQ-SIGTLLA 214

Query: 890 PIMF----HVGIIAIFGLYYCYW 946
           P+      ++GI  IFG Y  +W
Sbjct: 215 PLFSAADGYLGITIIFGAYALFW 237
>sp|Q19537|RFA1_CAEEL Probable replication factor A 73 kDa subunit (RP-A) (RF-A)
           (Replication factor-A protein 1)
          Length = 655

 Score = 31.6 bits (70), Expect = 4.2
 Identities = 18/64 (28%), Positives = 31/64 (48%)
 Frame = +2

Query: 413 VKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKMELVVGDSMIETPGN 592
           ++ T FN   E ++    +    Y     + ++NK F+N   DY++ L   DS+IE  G 
Sbjct: 271 IRCTAFNEVAESLYTTITENLSYYLSGGSVKQANKKFNNTGHDYEITL-RSDSIIEAGGE 329

Query: 593 WIFA 604
            + A
Sbjct: 330 LLAA 333
>sp|P41003|SMC2_SCHPO Structural maintenance of chromosome 2 (Chromosome segregation
            protein cut14) (Cell untimely torn protein 14)
          Length = 1172

 Score = 30.8 bits (68), Expect = 7.1
 Identities = 55/277 (19%), Positives = 109/277 (39%), Gaps = 15/277 (5%)
 Frame = +2

Query: 17   ETVKNVQSATTVLSRKSLVFDRTTGIYELDLT-------SVQEQLAKGF--YSIKLDMKT 169
            + VK+++     L R  L  D+     E D+        S   +L K F  Y  KLD  T
Sbjct: 751  DDVKDLKQRLPELDRLILQSDQAIKKIERDMQEWKHNKGSKMAELEKEFNQYKHKLDEFT 810

Query: 170  N--ETRYNEAVGVALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNW 343
               E   N+  GV L  + + +  ++N + S+ + +  T ++  ++              
Sbjct: 811  PILEKSENDYNGVKLECEQL-EGELQNHQQSLVQGESTTSLIKTEIA------------- 856

Query: 344  HHRLDMKFVIRDHSQSVKPHQVFVKLTHFN-TKQEIIFVVEADRTDVYKFN---LDLDKS 511
               L++  V  +H++      + ++   F+   +EI  +  + +T   + N   L + K 
Sbjct: 857  --ELELSLVNEEHNRKKLTELIEIESAKFSGLNKEIDSLSTSMKTFESEINNGELTIQKL 914

Query: 512  NKDFDNLSGDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSV 691
            N +FD L  + K   +   + +E   +WI    +         +   ++ R       ++
Sbjct: 915  NHEFDRLERE-KSVAITAINHLEKENDWIDGQKQHFGKQGTIFDFHSQNMRQCREQLHNL 973

Query: 692  VNPRIGSGPSLVKPQIFHMFKPAEKRGGQLISTVFTI 802
              PR  S    + P++  M    EK+  +L S + TI
Sbjct: 974  -KPRFASMRKAINPKVMDMIDGVEKKEAKLRSMIKTI 1009
>sp|O67151|GCSH1_AQUAE Glycine cleavage system H protein 1
          Length = 143

 Score = 30.4 bits (67), Expect = 9.3
 Identities = 15/44 (34%), Positives = 23/44 (52%)
 Frame = +2

Query: 71  VFDRTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETRYNEAVGV 202
           V D   GIY + + S+   LA   YSIK+     +  Y+EA+ +
Sbjct: 19  VKDEGNGIYSVGMASILAALAYPLYSIKIKPVGTKLEYDEALAI 62
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 135,174,053
Number of Sequences: 369166
Number of extensions: 2966107
Number of successful extensions: 8264
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7787
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8236
length of database: 68,354,980
effective HSP length: 112
effective length of database: 47,664,660
effective search space used: 11963829660
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00581

  1. Dr_sW_015_D17
  2. Dr_sW_026_K07
  3. Dr_sW_021_G11
  4. Dr_sW_015_J19
  5. Dr_sW_001_H11