Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_J19
(738 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9DBG6|RPN2_MOUSE Dolichyl-diphosphooligosaccharide--pro... 155 1e-37
sp|P04844|RIB2_HUMAN Dolichyl-diphosphooligosaccharide--pro... 154 3e-37
sp|P25235|RIB2_RAT Dolichyl-diphosphooligosaccharide--prote... 154 3e-37
sp|Q02795|OSTD_YEAST Dolichyl-diphosphooligosaccharide--pro... 35 0.21
sp|O68433|LEP4_LEGPN Type 4 prepilin-like proteins leader p... 35 0.21
sp|P50976|PTLCB_STRMU PTS system lactose-specific EIICB com... 32 2.3
sp|Q19537|RFA1_CAEEL Probable replication factor A 73 kDa s... 32 2.3
sp|P22543|VPS34_YEAST Phosphatidylinositol 3-kinase VPS34 (... 30 6.7
sp|P12050|FAND_ECOLI Outer membrane usher protein fanD prec... 30 6.7
sp|O48089|CYB_LACVV Cytochrome b 30 6.7
>sp|Q9DBG6|RPN2_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
subunit precursor (Ribophorin II) (RPN-II)
Length = 631
Score = 155 bits (391), Expect = 1e-37
Identities = 87/217 (40%), Positives = 124/217 (57%)
Frame = +1
Query: 43 PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKMELVVGDSMI 222
PHQ FV+L + T QE++FV E D +VYKF LD + +FD+ SG Y + L++GD+ +
Sbjct: 431 PHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATL 490
Query: 223 ETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSGPSLVKPQIFHMFKP 402
+ P W A++ + P E+ TV +S+ P K +I H+F+
Sbjct: 491 KNPILWNVADVVIKFPEE-------EAPSTVLS--QSLFTP---------KQEIQHLFRE 532
Query: 403 AEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYY 582
EKR ++S FT L I+ P +L+ ALW+ +G NVSNF+ S ++FH+G A+ GL Y
Sbjct: 533 PEKRPPTVVSNTFTAL-ILSPLLLLFALWIRIGANVSNFTFAPSTVIFHLGHAAMLGLMY 591
Query: 583 CYWGYLNMFTTLQCLLPLGMFTAFFGNRVLRQIASKR 693
YW LNMF TL+ L LG T GNR+L Q A KR
Sbjct: 592 IYWTQLNMFQTLKYLAVLGTVTFLAGNRMLAQHAVKR 628
>sp|P04844|RIB2_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
subunit precursor (Ribophorin II) (RPN-II) (RIBIIR)
Length = 631
Score = 154 bits (388), Expect = 3e-37
Identities = 87/217 (40%), Positives = 124/217 (57%)
Frame = +1
Query: 43 PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKMELVVGDSMI 222
PHQ FV+L + T QE++FV E D +VYKF LD + +FD+ SG Y + L++GD+ +
Sbjct: 431 PHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATL 490
Query: 223 ETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSGPSLVKPQIFHMFKP 402
+ P W A++ + P E+ TV +++ P K +I H+F+
Sbjct: 491 KNPILWNVADVVIKFPEE-------EAPSTVLS--QNLFTP---------KQEIQHLFRE 532
Query: 403 AEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYY 582
EKR ++S FT L I+ P +L+ ALW+ +G NVSNF+ S I+FH+G A+ GL Y
Sbjct: 533 PEKRPPTVVSNTFTAL-ILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMY 591
Query: 583 CYWGYLNMFTTLQCLLPLGMFTAFFGNRVLRQIASKR 693
YW LNMF TL+ L LG T GNR+L Q A KR
Sbjct: 592 VYWTQLNMFQTLKYLAILGSVTFLAGNRMLAQQAVKR 628
>sp|P25235|RIB2_RAT Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
subunit precursor (Ribophorin II) (RPN-II)
Length = 631
Score = 154 bits (388), Expect = 3e-37
Identities = 86/217 (39%), Positives = 124/217 (57%)
Frame = +1
Query: 43 PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKMELVVGDSMI 222
PHQ FV+L + T QE++FV E D +VYKF LD + +FD+ SG Y + L++GD+ +
Sbjct: 431 PHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATL 490
Query: 223 ETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSGPSLVKPQIFHMFKP 402
+ P W A++ + P E+ TV +++ P K +I H+F+
Sbjct: 491 KNPILWNVADVVIKFPEE-------EAPSTVLS--QNLFTP---------KQEIQHLFRE 532
Query: 403 AEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYY 582
EKR ++S FT L I+ P +L+ ALW+ +G NVSNF+ S ++FH+G A+ GL Y
Sbjct: 533 PEKRPPTVVSNTFTAL-ILSPLLLLFALWIRIGANVSNFTFAPSTVIFHLGHAAMLGLMY 591
Query: 583 CYWGYLNMFTTLQCLLPLGMFTAFFGNRVLRQIASKR 693
YW LNMF TL+ L LG T GNR+L Q A KR
Sbjct: 592 VYWTQLNMFQTLKYLAVLGTVTFLAGNRMLAQQAVKR 628
>sp|Q02795|OSTD_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase
delta subunit precursor (Oligosaccharyl transferase
delta subunit)
Length = 286
Score = 35.0 bits (79), Expect = 0.21
Identities = 24/93 (25%), Positives = 43/93 (46%), Gaps = 3/93 (3%)
Frame = +1
Query: 370 VKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMFH 549
+KP+I H+F KR + I+ +F ++ + L+V +N G++ + F
Sbjct: 176 IKPEIHHIFHAEPKRVAKPIAVIFVLIIFITILSLIVTWLNSCAAAFNNIPTGVTAVYFL 235
Query: 550 VGIIAIFG---LYYCYWGYLNMFTTLQCLLPLG 639
I I G ++ Y+ ++F TL L LG
Sbjct: 236 GFIATIVGFEVIFARYYLGTSIFETLFSSLYLG 268
>sp|O68433|LEP4_LEGPN Type 4 prepilin-like proteins leader peptide processing enzyme
[Includes: Leader peptidase (Prepilin peptidase);
N-methyltransferase ]
Length = 287
Score = 35.0 bits (79), Expect = 0.21
Identities = 24/84 (28%), Positives = 40/84 (47%), Gaps = 7/84 (8%)
Frame = +1
Query: 424 LISTVFTILC--IVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGY 597
LIS VF L ++P + + LW+ + N N V L V +++ G Y W +
Sbjct: 141 LISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNVFVSLD-----VAVLSCAGAYLALWLF 195
Query: 598 LNMFTTLQCLLPLG-----MFTAF 654
+N+F + C + +G +F AF
Sbjct: 196 INLFYLMTCKVCMGHGDFKLFAAF 219
>sp|P50976|PTLCB_STRMU PTS system lactose-specific EIICB component (EIICB-Lac) (EII-Lac)
[Includes: Lactose permease IIC component (PTS system
lactose-specific EIIC component); Lactose-specific
phosphotransferase enzyme IIB component (PTS system
lactose-specific EIIB component)]
Length = 568
Score = 31.6 bits (70), Expect = 2.3
Identities = 24/83 (28%), Positives = 42/83 (50%), Gaps = 6/83 (7%)
Frame = +1
Query: 361 PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVG--LS 534
P V P I +FK + F+I+ + + + +A+ ++GVNV+ S+G L+
Sbjct: 166 PEEVPPNISQVFK-------DIFPFAFSIIIL---YAIQLAIKAVIGVNVAQ-SIGTLLA 214
Query: 535 PIMF----HVGIIAIFGLYYCYW 591
P+ ++GI IFG Y +W
Sbjct: 215 PLFSAADGYLGITIIFGAYALFW 237
>sp|Q19537|RFA1_CAEEL Probable replication factor A 73 kDa subunit (RP-A) (RF-A)
(Replication factor-A protein 1)
Length = 655
Score = 31.6 bits (70), Expect = 2.3
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = +1
Query: 58 VKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKMELVVGDSMIETPGN 237
++ T FN E ++ + Y + ++NK F+N DY++ L DS+IE G
Sbjct: 271 IRCTAFNEVAESLYTTITENLSYYLSGGSVKQANKKFNNTGHDYEITL-RSDSIIEAGGE 329
Query: 238 WIFA 249
+ A
Sbjct: 330 LLAA 333
>sp|P22543|VPS34_YEAST Phosphatidylinositol 3-kinase VPS34 (PI3-kinase) (PtdIns-3-kinase)
(Vacuolar sorting protein 34) (Vacuolar protein
targeting protein 29)
Length = 875
Score = 30.0 bits (66), Expect = 6.7
Identities = 25/77 (32%), Positives = 38/77 (49%), Gaps = 7/77 (9%)
Frame = +1
Query: 148 DKSNKDFDNLSGDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGS- 324
DKSN +F + +L GDSM+ + + AN KL S +E +G + S
Sbjct: 424 DKSNSEFTIVDAVSSQKLS-GDSMLLSTSH---ANQKLLKSISSESETSGTESLPIVISP 479
Query: 325 ------KRSVVNPRIGS 357
+R++VNPR+GS
Sbjct: 480 LAEFLIRRALVNPRLGS 496
>sp|P12050|FAND_ECOLI Outer membrane usher protein fanD precursor
Length = 783
Score = 30.0 bits (66), Expect = 6.7
Identities = 27/110 (24%), Positives = 46/110 (41%), Gaps = 7/110 (6%)
Frame = +1
Query: 130 KFNLDLDKSNKDFDNLSGDYKMELVVGDSMIETP--GNWIFANIKLDMPSSPSTENTGES 303
KF D ++ +L GD L++GD+ T N F ++L ++ + E G+
Sbjct: 202 KFTTDDVTLSRAIKSLEGD----LIIGDTYTNTSLMDNISFYGVQLRSNNAMTPERRGDY 257
Query: 304 RRTVAGSKRSVVNPRIGSG-----PSLVKPQIFHMFKPAEKRGGQLISTV 438
++G +S + LV P FH+ R G+L+ TV
Sbjct: 258 SPIISGIAKSNARVTVKQNGVVLHSELVSPGPFHINNVRGIRSGELVMTV 307
>sp|O48089|CYB_LACVV Cytochrome b
Length = 380
Score = 30.0 bits (66), Expect = 6.7
Identities = 20/58 (34%), Positives = 30/58 (51%)
Frame = +1
Query: 487 WLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGYLNMFTTLQCLLPLGMFTAFFG 660
WL+ ++ ++ S+ I H+G GLYY + Y ++ LL L M TAFFG
Sbjct: 78 WLIRNLHANSASMFFICIYLHIGR----GLYYGSYIYTETWSIGVLLLLLVMATAFFG 131
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,261,934
Number of Sequences: 369166
Number of extensions: 2031310
Number of successful extensions: 6121
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5832
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6097
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6631293200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)