Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_K07
(803 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9DBG6|RPN2_MOUSE Dolichyl-diphosphooligosaccharide--pro... 154 3e-37
sp|P04844|RIB2_HUMAN Dolichyl-diphosphooligosaccharide--pro... 153 5e-37
sp|P25235|RIB2_RAT Dolichyl-diphosphooligosaccharide--prote... 153 6e-37
sp|Q02795|OSTD_YEAST Dolichyl-diphosphooligosaccharide--pro... 34 0.41
sp|Q6BS08|ATG1_DEBHA Serine/threonine-protein kinase ATG1 (... 33 1.2
sp|O68433|LEP4_LEGPN Type 4 prepilin-like proteins leader p... 33 1.2
sp|P50976|PTLCB_STRMU PTS system lactose-specific EIICB com... 32 2.7
sp|Q19537|RFA1_CAEEL Probable replication factor A 73 kDa s... 32 2.7
sp|P22543|VPS34_YEAST Phosphatidylinositol 3-kinase VPS34 (... 30 7.7
sp|P12050|FAND_ECOLI Outer membrane usher protein fanD prec... 30 7.7
>sp|Q9DBG6|RPN2_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
subunit precursor (Ribophorin II) (RPN-II)
Length = 631
Score = 154 bits (389), Expect = 3e-37
Identities = 92/263 (34%), Positives = 141/263 (53%), Gaps = 2/263 (0%)
Frame = +2
Query: 14 VALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDMKFVIRD 193
V L VK+ +V I NV+LS ++DQ ++T + + H + F + D
Sbjct: 363 VELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVD 422
Query: 194 HSQSVK--PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKME 367
+ + PHQ FV+L + T QE++FV E D +VYKF LD + +FD+ SG Y +
Sbjct: 423 VNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLY 482
Query: 368 LVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSGPSLVKP 547
L++GD+ ++ P W A++ + P E+ TV +S+ P K
Sbjct: 483 LIIGDATLKNPILWNVADVVIKFPEE-------EAPSTVL--SQSLFTP---------KQ 524
Query: 548 QIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGI 727
+I H+F+ EKR ++S FT L I+ P +L+ ALW+ +G NVSNF+ S ++FH+G
Sbjct: 525 EIQHLFREPEKRPPTVVSNTFTAL-ILSPLLLLFALWIRIGANVSNFTFAPSTVIFHLGH 583
Query: 728 IAIFGLYYCYWGYLNMFTTLQCL 796
A+ GL Y YW LNMF TL+ L
Sbjct: 584 AAMLGLMYIYWTQLNMFQTLKYL 606
>sp|P04844|RIB2_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
subunit precursor (Ribophorin II) (RPN-II) (RIBIIR)
Length = 631
Score = 153 bits (387), Expect = 5e-37
Identities = 92/263 (34%), Positives = 141/263 (53%), Gaps = 2/263 (0%)
Frame = +2
Query: 14 VALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDMKFVIRD 193
V L VK+ +V I NV+LS ++DQ ++T + + H + F + D
Sbjct: 363 VELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVD 422
Query: 194 HSQSVK--PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKME 367
+ + PHQ FV+L + T QE++FV E D +VYKF LD + +FD+ SG Y +
Sbjct: 423 VNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLY 482
Query: 368 LVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSGPSLVKP 547
L++GD+ ++ P W A++ + P E+ TV +++ P K
Sbjct: 483 LIIGDATLKNPILWNVADVVIKFPEE-------EAPSTVL--SQNLFTP---------KQ 524
Query: 548 QIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGI 727
+I H+F+ EKR ++S FT L I+ P +L+ ALW+ +G NVSNF+ S I+FH+G
Sbjct: 525 EIQHLFREPEKRPPTVVSNTFTAL-ILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGH 583
Query: 728 IAIFGLYYCYWGYLNMFTTLQCL 796
A+ GL Y YW LNMF TL+ L
Sbjct: 584 AAMLGLMYVYWTQLNMFQTLKYL 606
>sp|P25235|RIB2_RAT Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
subunit precursor (Ribophorin II) (RPN-II)
Length = 631
Score = 153 bits (386), Expect = 6e-37
Identities = 91/263 (34%), Positives = 141/263 (53%), Gaps = 2/263 (0%)
Frame = +2
Query: 14 VALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDMKFVIRD 193
V L VK+ +V I NV+LS ++DQ ++T + + H + F + D
Sbjct: 363 VELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVD 422
Query: 194 HSQSVK--PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKME 367
+ + PHQ FV+L + T QE++FV E D +VYKF LD + +FD+ SG Y +
Sbjct: 423 VNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLY 482
Query: 368 LVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSGPSLVKP 547
L++GD+ ++ P W A++ + P E+ TV +++ P K
Sbjct: 483 LIIGDATLKNPILWNVADVVIKFPEE-------EAPSTVL--SQNLFTP---------KQ 524
Query: 548 QIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGI 727
+I H+F+ EKR ++S FT L I+ P +L+ ALW+ +G NVSNF+ S ++FH+G
Sbjct: 525 EIQHLFREPEKRPPTVVSNTFTAL-ILSPLLLLFALWIRIGANVSNFTFAPSTVIFHLGH 583
Query: 728 IAIFGLYYCYWGYLNMFTTLQCL 796
A+ GL Y YW LNMF TL+ L
Sbjct: 584 AAMLGLMYVYWTQLNMFQTLKYL 606
>sp|Q02795|OSTD_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase
delta subunit precursor (Oligosaccharyl transferase
delta subunit)
Length = 286
Score = 34.3 bits (77), Expect = 0.41
Identities = 20/77 (25%), Positives = 34/77 (44%), Gaps = 6/77 (7%)
Frame = +2
Query: 539 VKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMF- 715
+KP+I H+F KR + I+ +F ++ + L+V +N G++ + F
Sbjct: 176 IKPEIHHIFHAEPKRVAKPIAVIFVLIIFITILSLIVTWLNSCAAAFNNIPTGVTAVYFL 235
Query: 716 -----HVGIIAIFGLYY 751
VG IF YY
Sbjct: 236 GFIATIVGFEVIFARYY 252
>sp|Q6BS08|ATG1_DEBHA Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1)
Length = 875
Score = 32.7 bits (73), Expect = 1.2
Identities = 27/87 (31%), Positives = 42/87 (48%), Gaps = 9/87 (10%)
Frame = +2
Query: 2 EAVGVALVV--KVICQVSIENVELSIK-----ERDQITGILNYKLTMG--SLLSDKLSMN 154
E+V + VV K+ + +EN+E+ I + I G+L+YK T L+ D SM
Sbjct: 46 ESVAIKSVVRSKLKSKKLVENLEIEISILKTMKHPHIVGLLDYKQTTSHFHLVMDYCSMG 105
Query: 155 WHHRLDMKFVIRDHSQSVKPHQVFVKL 235
D+ + IR +Q +K H V L
Sbjct: 106 -----DLSYFIRKRNQLIKTHPVISSL 127
>sp|O68433|LEP4_LEGPN Type 4 prepilin-like proteins leader peptide processing enzyme
[Includes: Leader peptidase (Prepilin peptidase);
N-methyltransferase ]
Length = 287
Score = 32.7 bits (73), Expect = 1.2
Identities = 20/69 (28%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +2
Query: 593 LISTVFTILC--IVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGY 766
LIS VF L ++P + + LW+ + N N V L V +++ G Y W +
Sbjct: 141 LISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNVFVSLD-----VAVLSCAGAYLALWLF 195
Query: 767 LNMFTTLQC 793
+N+F + C
Sbjct: 196 INLFYLMTC 204
>sp|P50976|PTLCB_STRMU PTS system lactose-specific EIICB component (EIICB-Lac) (EII-Lac)
[Includes: Lactose permease IIC component (PTS system
lactose-specific EIIC component); Lactose-specific
phosphotransferase enzyme IIB component (PTS system
lactose-specific EIIB component)]
Length = 568
Score = 31.6 bits (70), Expect = 2.7
Identities = 24/83 (28%), Positives = 42/83 (50%), Gaps = 6/83 (7%)
Frame = +2
Query: 530 PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVG--LS 703
P V P I +FK + F+I+ + + + +A+ ++GVNV+ S+G L+
Sbjct: 166 PEEVPPNISQVFK-------DIFPFAFSIIIL---YAIQLAIKAVIGVNVAQ-SIGTLLA 214
Query: 704 PIMF----HVGIIAIFGLYYCYW 760
P+ ++GI IFG Y +W
Sbjct: 215 PLFSAADGYLGITIIFGAYALFW 237
>sp|Q19537|RFA1_CAEEL Probable replication factor A 73 kDa subunit (RP-A) (RF-A)
(Replication factor-A protein 1)
Length = 655
Score = 31.6 bits (70), Expect = 2.7
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = +2
Query: 227 VKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKMELVVGDSMIETPGN 406
++ T FN E ++ + Y + ++NK F+N DY++ L DS+IE G
Sbjct: 271 IRCTAFNEVAESLYTTITENLSYYLSGGSVKQANKKFNNTGHDYEITL-RSDSIIEAGGE 329
Query: 407 WIFA 418
+ A
Sbjct: 330 LLAA 333
>sp|P22543|VPS34_YEAST Phosphatidylinositol 3-kinase VPS34 (PI3-kinase) (PtdIns-3-kinase)
(Vacuolar sorting protein 34) (Vacuolar protein
targeting protein 29)
Length = 875
Score = 30.0 bits (66), Expect = 7.7
Identities = 25/77 (32%), Positives = 38/77 (49%), Gaps = 7/77 (9%)
Frame = +2
Query: 317 DKSNKDFDNLSGDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGS- 493
DKSN +F + +L GDSM+ + + AN KL S +E +G + S
Sbjct: 424 DKSNSEFTIVDAVSSQKLS-GDSMLLSTSH---ANQKLLKSISSESETSGTESLPIVISP 479
Query: 494 ------KRSVVNPRIGS 526
+R++VNPR+GS
Sbjct: 480 LAEFLIRRALVNPRLGS 496
>sp|P12050|FAND_ECOLI Outer membrane usher protein fanD precursor
Length = 783
Score = 30.0 bits (66), Expect = 7.7
Identities = 27/110 (24%), Positives = 46/110 (41%), Gaps = 7/110 (6%)
Frame = +2
Query: 299 KFNLDLDKSNKDFDNLSGDYKMELVVGDSMIETP--GNWIFANIKLDMPSSPSTENTGES 472
KF D ++ +L GD L++GD+ T N F ++L ++ + E G+
Sbjct: 202 KFTTDDVTLSRAIKSLEGD----LIIGDTYTNTSLMDNISFYGVQLRSNNAMTPERRGDY 257
Query: 473 RRTVAGSKRSVVNPRIGSG-----PSLVKPQIFHMFKPAEKRGGQLISTV 607
++G +S + LV P FH+ R G+L+ TV
Sbjct: 258 SPIISGIAKSNARVTVKQNGVVLHSELVSPGPFHINNVRGIRSGELVMTV 307
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,045,139
Number of Sequences: 369166
Number of extensions: 2119206
Number of successful extensions: 5819
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5531
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5804
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7618580670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)