Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_021_G11
(527 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9DBG6|RPN2_MOUSE Dolichyl-diphosphooligosaccharide--pro... 111 9e-25
sp|P04844|RIB2_HUMAN Dolichyl-diphosphooligosaccharide--pro... 110 2e-24
sp|P25235|RIB2_RAT Dolichyl-diphosphooligosaccharide--prote... 110 2e-24
sp|Q02795|OSTD_YEAST Dolichyl-diphosphooligosaccharide--pro... 35 0.11
sp|O68433|LEP4_LEGPN Type 4 prepilin-like proteins leader p... 35 0.11
sp|P50976|PTLCB_STRMU PTS system lactose-specific EIICB com... 32 1.2
sp|P22543|VPS34_YEAST Phosphatidylinositol 3-kinase VPS34 (... 30 3.6
sp|O48089|CYB_LACVV Cytochrome b 30 3.6
sp|Q8ZGC6|LOLA_YERPE Outer-membrane lipoprotein carrier pro... 30 4.7
sp|Q36427|CYB_LOCMI Cytochrome b 30 4.7
>sp|Q9DBG6|RPN2_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63
kDa subunit precursor (Ribophorin II) (RPN-II)
Length = 631
Score = 111 bits (278), Expect = 9e-25
Identities = 66/177 (37%), Positives = 98/177 (55%), Gaps = 2/177 (1%)
Frame = -2
Query: 526 DLDKSNK--DFDNLSGDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTV 353
+LD S + +FD+ SG Y + L++GD+ ++ P W A++ + P E+ TV
Sbjct: 462 ELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNVADVVIKFPEE-------EAPSTV 514
Query: 352 AGSKRSVVNPRIGSGPSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLM 173
+S+ P K +I H+F+ EKR ++S FT L I+ P +L+ ALW+
Sbjct: 515 LS--QSLFTP---------KQEIQHLFREPEKRPPTVVSNTFTAL-ILSPLLLLFALWIR 562
Query: 172 VGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGYLNMFTTLQCLLPLGMFTAFFGNR 2
+G NVSNF+ S ++FH+G A+ GL Y YW LNMF TL+ L LG T GNR
Sbjct: 563 IGANVSNFTFAPSTVIFHLGHAAMLGLMYIYWTQLNMFQTLKYLAVLGTVTFLAGNR 619
>sp|P04844|RIB2_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63
kDa subunit precursor (Ribophorin II) (RPN-II) (RIBIIR)
Length = 631
Score = 110 bits (275), Expect = 2e-24
Identities = 66/177 (37%), Positives = 98/177 (55%), Gaps = 2/177 (1%)
Frame = -2
Query: 526 DLDKSNK--DFDNLSGDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTV 353
+LD S + +FD+ SG Y + L++GD+ ++ P W A++ + P E+ TV
Sbjct: 462 ELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNVADVVIKFPEE-------EAPSTV 514
Query: 352 AGSKRSVVNPRIGSGPSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLM 173
+++ P K +I H+F+ EKR ++S FT L I+ P +L+ ALW+
Sbjct: 515 LS--QNLFTP---------KQEIQHLFREPEKRPPTVVSNTFTAL-ILSPLLLLFALWIR 562
Query: 172 VGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGYLNMFTTLQCLLPLGMFTAFFGNR 2
+G NVSNF+ S I+FH+G A+ GL Y YW LNMF TL+ L LG T GNR
Sbjct: 563 IGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKYLAILGSVTFLAGNR 619
>sp|P25235|RIB2_RAT Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63
kDa subunit precursor (Ribophorin II) (RPN-II)
Length = 631
Score = 110 bits (275), Expect = 2e-24
Identities = 65/177 (36%), Positives = 98/177 (55%), Gaps = 2/177 (1%)
Frame = -2
Query: 526 DLDKSNK--DFDNLSGDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTV 353
+LD S + +FD+ SG Y + L++GD+ ++ P W A++ + P E+ TV
Sbjct: 462 ELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNVADVVIKFPEE-------EAPSTV 514
Query: 352 AGSKRSVVNPRIGSGPSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLM 173
+++ P K +I H+F+ EKR ++S FT L I+ P +L+ ALW+
Sbjct: 515 LS--QNLFTP---------KQEIQHLFREPEKRPPTVVSNTFTAL-ILSPLLLLFALWIR 562
Query: 172 VGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGYLNMFTTLQCLLPLGMFTAFFGNR 2
+G NVSNF+ S ++FH+G A+ GL Y YW LNMF TL+ L LG T GNR
Sbjct: 563 IGANVSNFTFAPSTVIFHLGHAAMLGLMYVYWTQLNMFQTLKYLAVLGTVTFLAGNR 619
>sp|Q02795|OSTD_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase
delta subunit precursor (Oligosaccharyl transferase
delta subunit)
Length = 286
Score = 35.0 bits (79), Expect = 0.11
Identities = 24/93 (25%), Positives = 43/93 (46%), Gaps = 3/93 (3%)
Frame = -2
Query: 298 VKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVGLSPIMFH 119
+KP+I H+F KR + I+ +F ++ + L+V +N G++ + F
Sbjct: 176 IKPEIHHIFHAEPKRVAKPIAVIFVLIIFITILSLIVTWLNSCAAAFNNIPTGVTAVYFL 235
Query: 118 VGIIAIFG---LYYCYWGYLNMFTTLQCLLPLG 29
I I G ++ Y+ ++F TL L LG
Sbjct: 236 GFIATIVGFEVIFARYYLGTSIFETLFSSLYLG 268
>sp|O68433|LEP4_LEGPN Type 4 prepilin-like proteins leader peptide processing enzyme
[Includes: Leader peptidase (Prepilin peptidase);
N-methyltransferase ]
Length = 287
Score = 35.0 bits (79), Expect = 0.11
Identities = 24/84 (28%), Positives = 40/84 (47%), Gaps = 7/84 (8%)
Frame = -2
Query: 244 LISTVFTILC--IVPPFILMVALWLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGY 71
LIS VF L ++P + + LW+ + N N V L V +++ G Y W +
Sbjct: 141 LISLVFIDLDHQLLPDSLTLGLLWIGLIANTQNVFVSLD-----VAVLSCAGAYLALWLF 195
Query: 70 LNMFTTLQCLLPLG-----MFTAF 14
+N+F + C + +G +F AF
Sbjct: 196 INLFYLMTCKVCMGHGDFKLFAAF 219
>sp|P50976|PTLCB_STRMU PTS system lactose-specific EIICB component (EIICB-Lac) (EII-Lac)
[Includes: Lactose permease IIC component (PTS system
lactose-specific EIIC component); Lactose-specific
phosphotransferase enzyme IIB component (PTS system
lactose-specific EIIB component)]
Length = 568
Score = 31.6 bits (70), Expect = 1.2
Identities = 24/83 (28%), Positives = 42/83 (50%), Gaps = 6/83 (7%)
Frame = -2
Query: 307 PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVPPFILMVALWLMVGVNVSNFSVG--LS 134
P V P I +FK + F+I+ + + + +A+ ++GVNV+ S+G L+
Sbjct: 166 PEEVPPNISQVFK-------DIFPFAFSIIIL---YAIQLAIKAVIGVNVAQ-SIGTLLA 214
Query: 133 PIMF----HVGIIAIFGLYYCYW 77
P+ ++GI IFG Y +W
Sbjct: 215 PLFSAADGYLGITIIFGAYALFW 237
>sp|P22543|VPS34_YEAST Phosphatidylinositol 3-kinase VPS34 (PI3-kinase) (PtdIns-3-kinase)
(Vacuolar sorting protein 34) (Vacuolar protein
targeting protein 29)
Length = 875
Score = 30.0 bits (66), Expect = 3.6
Identities = 25/77 (32%), Positives = 38/77 (49%), Gaps = 7/77 (9%)
Frame = -2
Query: 520 DKSNKDFDNLSGDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGS- 344
DKSN +F + +L GDSM+ + + AN KL S +E +G + S
Sbjct: 424 DKSNSEFTIVDAVSSQKLS-GDSMLLSTSH---ANQKLLKSISSESETSGTESLPIVISP 479
Query: 343 ------KRSVVNPRIGS 311
+R++VNPR+GS
Sbjct: 480 LAEFLIRRALVNPRLGS 496
>sp|O48089|CYB_LACVV Cytochrome b
Length = 380
Score = 30.0 bits (66), Expect = 3.6
Identities = 20/58 (34%), Positives = 30/58 (51%)
Frame = -2
Query: 181 WLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGYLNMFTTLQCLLPLGMFTAFFG 8
WL+ ++ ++ S+ I H+G GLYY + Y ++ LL L M TAFFG
Sbjct: 78 WLIRNLHANSASMFFICIYLHIGR----GLYYGSYIYTETWSIGVLLLLLVMATAFFG 131
>sp|Q8ZGC6|LOLA_YERPE Outer-membrane lipoprotein carrier protein precursor
Length = 202
Score = 29.6 bits (65), Expect = 4.7
Identities = 21/68 (30%), Positives = 31/68 (45%)
Frame = -1
Query: 314 FRSILGETANLSHVQTS*ETRRSIDINSVHNSVHCAPVYSDGGAVVDGRC*CVEFQCWIE 135
F S L + L+ T + R S +NS H S A SDG V +G E + W++
Sbjct: 10 FLSGLISASALADASTDLQNRLS-KVNSFHASFSQAVTSSDGAVVQEG-----EGELWVK 63
Query: 134 SNHVSRWH 111
++ WH
Sbjct: 64 RPNLFNWH 71
>sp|Q36427|CYB_LOCMI Cytochrome b
Length = 379
Score = 29.6 bits (65), Expect = 4.7
Identities = 19/58 (32%), Positives = 30/58 (51%)
Frame = -2
Query: 181 WLMVGVNVSNFSVGLSPIMFHVGIIAIFGLYYCYWGYLNMFTTLQCLLPLGMFTAFFG 8
WL+ ++ + S+ + HVG G+YY + Y+N + T +L L M TAF G
Sbjct: 78 WLIRTLHANGASMFFICMYLHVGR----GIYYGSYMYMNTWMTGTIILFLVMATAFMG 131
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,372,129
Number of Sequences: 369166
Number of extensions: 1559376
Number of successful extensions: 4771
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4563
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4748
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3551563800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)