Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_D17
(820 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P25235|RIB2_RAT Dolichyl-diphosphooligosaccharide--prote... 112 9e-25
sp|Q9DBG6|RPN2_MOUSE Dolichyl-diphosphooligosaccharide--pro... 112 2e-24
sp|P04844|RIB2_HUMAN Dolichyl-diphosphooligosaccharide--pro... 112 2e-24
sp|Q6BS08|ATG1_DEBHA Serine/threonine-protein kinase ATG1 (... 41 0.003
sp|Q19537|RFA1_CAEEL Probable replication factor A 73 kDa s... 32 2.7
sp|P41003|SMC2_SCHPO Structural maintenance of chromosome 2... 31 4.7
sp|O67151|GCSH1_AQUAE Glycine cleavage system H protein 1 30 6.1
sp|Q9LME2|Y1226_ARATH Hypothetical protein At1g22260 30 8.0
sp|P22543|VPS34_YEAST Phosphatidylinositol 3-kinase VPS34 (... 30 8.0
sp|P12050|FAND_ECOLI Outer membrane usher protein fanD prec... 30 8.0
>sp|P25235|RIB2_RAT Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
subunit precursor (Ribophorin II) (RPN-II)
Length = 631
Score = 112 bits (281), Expect = 9e-25
Identities = 82/274 (29%), Positives = 136/274 (49%), Gaps = 2/274 (0%)
Frame = +2
Query: 2 AALVAETVKNVQSATTVLSRKSLVFDRTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETR 181
AA+ E K+V S TVL + + F ++EL+ +V+ L G+Y + ++ + R
Sbjct: 303 AAVKLEHAKSVASRATVLQK--MPFSLVGDVFELNFKNVK--LPSGYYDFSVRVE-GDNR 357
Query: 182 YNEAVGVALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDM 361
Y A V L VK+ +V I NV+LS ++DQ ++T + + H +
Sbjct: 358 YI-ANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL 416
Query: 362 KFVIRDHSQSVK--PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLS 535
F + D + + PHQ FV+L + T QE++FV E D +VYKF LD + +FD+ S
Sbjct: 417 FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSAS 476
Query: 536 GDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSG 715
G Y + L++GD+ ++ P W A++ + P E+ TV +++ P
Sbjct: 477 GTYTLYLIIGDATLKNPILWNVADVVIKFPEE-------EAPSTVL--SQNLFTP----- 522
Query: 716 PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVP 817
K +I H+F+ EKR ++S FT L + P
Sbjct: 523 ----KQEIQHLFREPEKRPPTVVSNTFTALILSP 552
>sp|Q9DBG6|RPN2_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
subunit precursor (Ribophorin II) (RPN-II)
Length = 631
Score = 112 bits (279), Expect = 2e-24
Identities = 81/274 (29%), Positives = 136/274 (49%), Gaps = 2/274 (0%)
Frame = +2
Query: 2 AALVAETVKNVQSATTVLSRKSLVFDRTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETR 181
AA+ E K+ + TVL + F ++EL+ +V+ L+ G+Y + ++ ++R
Sbjct: 303 AAVKLEHAKSAATRATVLQKTP--FSLVGNVFELNFKNVK--LSSGYYDFSVRVE-GDSR 357
Query: 182 YNEAVGVALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDM 361
Y A V L VK+ +V I NV+LS ++DQ ++T + + H +
Sbjct: 358 YI-ANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL 416
Query: 362 KFVIRDHSQSVK--PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLS 535
F + D + + PHQ FV+L + T QE++FV E D +VYKF LD + +FD+ S
Sbjct: 417 FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSAS 476
Query: 536 GDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSG 715
G Y + L++GD+ ++ P W A++ + P E+ TV +S+ P
Sbjct: 477 GTYTLYLIIGDATLKNPILWNVADVVIKFPEE-------EAPSTVL--SQSLFTP----- 522
Query: 716 PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVP 817
K +I H+F+ EKR ++S FT L + P
Sbjct: 523 ----KQEIQHLFREPEKRPPTVVSNTFTALILSP 552
>sp|P04844|RIB2_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa
subunit precursor (Ribophorin II) (RPN-II) (RIBIIR)
Length = 631
Score = 112 bits (279), Expect = 2e-24
Identities = 81/274 (29%), Positives = 136/274 (49%), Gaps = 2/274 (0%)
Frame = +2
Query: 2 AALVAETVKNVQSATTVLSRKSLVFDRTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETR 181
A + E K+V S TVL + S F ++EL+ +V+ + G+Y ++++ + R
Sbjct: 303 ATVKLEHAKSVASRATVLQKTS--FTPVGDVFELNFMNVK--FSSGYYDFLVEVE-GDNR 357
Query: 182 YNEAVGVALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDM 361
Y A V L VK+ +V I NV+LS ++DQ ++T + + H +
Sbjct: 358 YI-ANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFAL 416
Query: 362 KFVIRDHSQSVK--PHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLS 535
F + D + + PHQ FV+L + T QE++FV E D +VYKF LD + +FD+ S
Sbjct: 417 FFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSAS 476
Query: 536 GDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSVVNPRIGSG 715
G Y + L++GD+ ++ P W A++ + P E+ TV +++ P
Sbjct: 477 GTYTLYLIIGDATLKNPILWNVADVVIKFPEE-------EAPSTVL--SQNLFTP----- 522
Query: 716 PSLVKPQIFHMFKPAEKRGGQLISTVFTILCIVP 817
K +I H+F+ EKR ++S FT L + P
Sbjct: 523 ----KQEIQHLFREPEKRPPTVVSNTFTALILSP 552
>sp|Q6BS08|ATG1_DEBHA Serine/threonine-protein kinase ATG1 (Autophagy-related protein 1)
Length = 875
Score = 41.2 bits (95), Expect = 0.003
Identities = 36/123 (29%), Positives = 59/123 (47%), Gaps = 9/123 (7%)
Frame = +2
Query: 80 RTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETRYNEAVGVALVV--KVICQVSIENVEL 253
+T G+Y S+ ++ KG ++ K T+ NE+V + VV K+ + +EN+E+
Sbjct: 17 KTIGVY-----SIGPEIGKGSFATVY--KCFNTKTNESVAIKSVVRSKLKSKKLVENLEI 69
Query: 254 SIK-----ERDQITGILNYKLTMG--SLLSDKLSMNWHHRLDMKFVIRDHSQSVKPHQVF 412
I + I G+L+YK T L+ D SM D+ + IR +Q +K H V
Sbjct: 70 EISILKTMKHPHIVGLLDYKQTTSHFHLVMDYCSMG-----DLSYFIRKRNQLIKTHPVI 124
Query: 413 VKL 421
L
Sbjct: 125 SSL 127
>sp|Q19537|RFA1_CAEEL Probable replication factor A 73 kDa subunit (RP-A) (RF-A)
(Replication factor-A protein 1)
Length = 655
Score = 31.6 bits (70), Expect = 2.7
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = +2
Query: 413 VKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGDYKMELVVGDSMIETPGN 592
++ T FN E ++ + Y + ++NK F+N DY++ L DS+IE G
Sbjct: 271 IRCTAFNEVAESLYTTITENLSYYLSGGSVKQANKKFNNTGHDYEITL-RSDSIIEAGGE 329
Query: 593 WIFA 604
+ A
Sbjct: 330 LLAA 333
>sp|P41003|SMC2_SCHPO Structural maintenance of chromosome 2 (Chromosome segregation
protein cut14) (Cell untimely torn protein 14)
Length = 1172
Score = 30.8 bits (68), Expect = 4.7
Identities = 55/277 (19%), Positives = 109/277 (39%), Gaps = 15/277 (5%)
Frame = +2
Query: 17 ETVKNVQSATTVLSRKSLVFDRTTGIYELDLT-------SVQEQLAKGF--YSIKLDMKT 169
+ VK+++ L R L D+ E D+ S +L K F Y KLD T
Sbjct: 751 DDVKDLKQRLPELDRLILQSDQAIKKIERDMQEWKHNKGSKMAELEKEFNQYKHKLDEFT 810
Query: 170 N--ETRYNEAVGVALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNW 343
E N+ GV L + + + ++N + S+ + + T ++ ++
Sbjct: 811 PILEKSENDYNGVKLECEQL-EGELQNHQQSLVQGESTTSLIKTEIA------------- 856
Query: 344 HHRLDMKFVIRDHSQSVKPHQVFVKLTHFN-TKQEIIFVVEADRTDVYKFN---LDLDKS 511
L++ V +H++ + ++ F+ +EI + + +T + N L + K
Sbjct: 857 --ELELSLVNEEHNRKKLTELIEIESAKFSGLNKEIDSLSTSMKTFESEINNGELTIQKL 914
Query: 512 NKDFDNLSGDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGSKRSV 691
N +FD L + K + + +E +WI + + ++ R ++
Sbjct: 915 NHEFDRLERE-KSVAITAINHLEKENDWIDGQKQHFGKQGTIFDFHSQNMRQCREQLHNL 973
Query: 692 VNPRIGSGPSLVKPQIFHMFKPAEKRGGQLISTVFTI 802
PR S + P++ M EK+ +L S + TI
Sbjct: 974 -KPRFASMRKAINPKVMDMIDGVEKKEAKLRSMIKTI 1009
>sp|O67151|GCSH1_AQUAE Glycine cleavage system H protein 1
Length = 143
Score = 30.4 bits (67), Expect = 6.1
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +2
Query: 71 VFDRTTGIYELDLTSVQEQLAKGFYSIKLDMKTNETRYNEAVGV 202
V D GIY + + S+ LA YSIK+ + Y+EA+ +
Sbjct: 19 VKDEGNGIYSVGMASILAALAYPLYSIKIKPVGTKLEYDEALAI 62
>sp|Q9LME2|Y1226_ARATH Hypothetical protein At1g22260
Length = 871
Score = 30.0 bits (66), Expect = 8.0
Identities = 45/197 (22%), Positives = 79/197 (40%), Gaps = 1/197 (0%)
Frame = +2
Query: 5 ALVAETVKNVQSATTVLSRKSLVFDRTTGIYEL-DLTSVQEQLAKGFYSIKLDMKTNETR 181
A AE N+++ + L + L + TT E+ DL S+QE+L K S++L
Sbjct: 218 ASAAERKLNIENLNSQLEKVHL--ELTTKEDEVKDLVSIQEKLEKEKTSVQLSADN---- 271
Query: 182 YNEAVGVALVVKVICQVSIENVELSIKERDQITGILNYKLTMGSLLSDKLSMNWHHRLDM 361
C + + E +K+ D++ L +LT DK ++ + + D
Sbjct: 272 --------------CFEKLVSSEQEVKKLDELVQYLVAELTE----LDKKNLTFKEKFDK 313
Query: 362 KFVIRDHSQSVKPHQVFVKLTHFNTKQEIIFVVEADRTDVYKFNLDLDKSNKDFDNLSGD 541
+ D TH I +++ DR +L LD++ + FDNL G+
Sbjct: 314 LSGLYD--------------TH-------IMLLQKDR------DLALDRAQRSFDNLQGE 346
Query: 542 YKMELVVGDSMIETPGN 592
+ +E+ GN
Sbjct: 347 L-FRVAATKEALESAGN 362
>sp|P22543|VPS34_YEAST Phosphatidylinositol 3-kinase VPS34 (PI3-kinase) (PtdIns-3-kinase)
(Vacuolar sorting protein 34) (Vacuolar protein
targeting protein 29)
Length = 875
Score = 30.0 bits (66), Expect = 8.0
Identities = 25/77 (32%), Positives = 38/77 (49%), Gaps = 7/77 (9%)
Frame = +2
Query: 503 DKSNKDFDNLSGDYKMELVVGDSMIETPGNWIFANIKLDMPSSPSTENTGESRRTVAGS- 679
DKSN +F + +L GDSM+ + + AN KL S +E +G + S
Sbjct: 424 DKSNSEFTIVDAVSSQKLS-GDSMLLSTSH---ANQKLLKSISSESETSGTESLPIVISP 479
Query: 680 ------KRSVVNPRIGS 712
+R++VNPR+GS
Sbjct: 480 LAEFLIRRALVNPRLGS 496
>sp|P12050|FAND_ECOLI Outer membrane usher protein fanD precursor
Length = 783
Score = 30.0 bits (66), Expect = 8.0
Identities = 27/110 (24%), Positives = 46/110 (41%), Gaps = 7/110 (6%)
Frame = +2
Query: 485 KFNLDLDKSNKDFDNLSGDYKMELVVGDSMIETP--GNWIFANIKLDMPSSPSTENTGES 658
KF D ++ +L GD L++GD+ T N F ++L ++ + E G+
Sbjct: 202 KFTTDDVTLSRAIKSLEGD----LIIGDTYTNTSLMDNISFYGVQLRSNNAMTPERRGDY 257
Query: 659 RRTVAGSKRSVVNPRIGSG-----PSLVKPQIFHMFKPAEKRGGQLISTV 793
++G +S + LV P FH+ R G+L+ TV
Sbjct: 258 SPIISGIAKSNARVTVKQNGVVLHSELVSPGPFHINNVRGIRSGELVMTV 307
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 98,099,249
Number of Sequences: 369166
Number of extensions: 2022142
Number of successful extensions: 5103
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4930
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5095
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7859674995
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)