Planaria EST Database


DrC_00359

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00359
         (804 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P55112|NAS4_CAEEL  Zinc metalloproteinase nas-4 precursor...   102   1e-21
sp|P31581|HCE21_ORYLA  High choriolytic enzyme 2 precursor (...   101   3e-21
sp|P31580|HCE23_ORYLA  High choriolytic enzyme 1 precursor (...    98   3e-20
sp|Q19269|NAS14_CAEEL  Zinc metalloproteinase nas-14 precurs...    95   3e-19
sp|P55115|NAS15_CAEEL  Zinc metalloproteinase nas-15 precurs...    93   7e-19
sp|P07584|ASTA_ASTFL  Astacin precursor (Crayfish small-mole...    93   1e-18
sp|P31579|LCE_ORYLA  Low choriolytic enzyme precursor (Hatch...    90   8e-18
sp|Q9U3S9|NAS6_CAEEL  Zinc metalloproteinase nas-6 precursor...    87   4e-17
sp|Q18439|NAS8_CAEEL  Zinc metalloproteinase nas-8 precursor...    87   5e-17
sp|Q20191|NAS13_CAEEL  Zinc metalloproteinase nas-13 precurs...    86   9e-17
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
          Length = 315

 Score =  102 bits (254), Expect = 1e-21
 Identities = 68/200 (34%), Positives = 105/200 (52%), Gaps = 13/200 (6%)
 Frame = +2

Query: 140 GDMLLDSTDIAII-------NGFKNHYANSKKWPNNVVLYSFAGNFPRDKIGAVRESLGE 298
           GD+LL+S    +        N  K  Y   ++WPNN + Y+ +  +       +  ++ E
Sbjct: 75  GDILLESPKKFVEENNKLGRNAIKQIY---RRWPNNEIPYTLSSQYGSYARSVIANAMNE 131

Query: 299 LQNDLRGCVKF---QESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTI 469
                + CVKF     S +  Y+ ++   EGCYSL+G TGG  QP++L+S GC+   GTI
Sbjct: 132 YHT--KTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG-KQPVSLDS-GCI-QVGTI 185

Query: 470 KHEFMHALGFMHTHMRKDRDQHISINWARILSSQCSQFVKCD---GCEPDGPYETESVMH 640
            HE MHA+GF H   R+DRD +I + W  +++    QF K +       D PY+  S+MH
Sbjct: 186 VHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMH 245

Query: 641 YNSEGFACNPGEKVVLKKNG 700
           Y    F+ +  + +V KK+G
Sbjct: 246 YGPYAFSGSGKKTLVPKKSG 265
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
           HCE 2 subunit) (Choriolysin H 2)
          Length = 279

 Score =  101 bits (251), Expect = 3e-21
 Identities = 61/176 (34%), Positives = 91/176 (51%), Gaps = 3/176 (1%)
 Frame = +2

Query: 227 VVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH-YIEVNSKQEGCYSLLGF 403
           V+ Y  +  + R ++  +  ++       R C++F   TN + +I V SK  GCYS LG 
Sbjct: 100 VIPYVISSQYSRGEVATIEGAMRAFNG--RTCIRFVRRTNEYDFISVVSKN-GCYSELGR 156

Query: 404 TGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILSSQCSQF 583
            GG  Q L+L   GC+YS G I+HE  HALGF H   R DRD ++ INW  I+ +    F
Sbjct: 157 KGG-QQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNF 214

Query: 584 VKCDGCEPDGPYETESVMHYNSEGFACNPGEKVV--LKKNGGAIGYNHGTTQNDLT 745
            K D    + PY+  S+MHY  + F+   G   +  +      IG  +G ++ D+T
Sbjct: 215 NKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDIT 270
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
           HCE 1 subunit) (Choriolysin H 1)
          Length = 270

 Score = 97.8 bits (242), Expect = 3e-20
 Identities = 62/186 (33%), Positives = 95/186 (51%), Gaps = 5/186 (2%)
 Frame = +2

Query: 209 KKWPNNVVL--YSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH-YIEVNSKQE 379
           KK  N +V+  Y  +  +   ++  +  ++       + C++F   TN + +I V SK  
Sbjct: 83  KKASNGLVVIPYVISSEYSGGEVATIEGAMRAFNG--KTCIRFVRRTNEYDFISVVSKT- 139

Query: 380 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARI 559
           GCYS LG  GG  Q L++   GC+YS G I+HE  HALGF H   R DRD ++ INW  I
Sbjct: 140 GCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENI 197

Query: 560 LSSQCSQFVKCDGCEPDGPYETESVMHYNSEGFACNPGEKVV--LKKNGGAIGYNHGTTQ 733
           + +    F K D    + PY+  S+MHY  + F+   G   +  +      IG  +G ++
Sbjct: 198 IPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSR 257

Query: 734 NDLTMI 751
            D+T I
Sbjct: 258 WDITRI 263
>sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precursor (Nematode astacin 14)
          Length = 503

 Score = 94.7 bits (234), Expect = 3e-19
 Identities = 62/183 (33%), Positives = 87/183 (47%), Gaps = 6/183 (3%)
 Frame = +2

Query: 209 KKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGHYIEVNSKQE--- 379
           K WP   V Y        D+  A+ ++  E +   + CV+F   T+  +  +  K+    
Sbjct: 123 KLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKT--KTCVRFVPKTDDDFDYIYVKRNVAF 180

Query: 380 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARI 559
           GC S +G  GG NQ ++LE   C +SKG I HE MHALGF H H R DRD  + IN   I
Sbjct: 181 GCSSYVGRAGG-NQTVSLEVDKC-FSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNI 238

Query: 560 LSSQCSQFVKCDGCEPDG---PYETESVMHYNSEGFACNPGEKVVLKKNGGAIGYNHGTT 730
                  F K      D    PY+ ESVMHY+   F+ N    ++ K N   +G  +  +
Sbjct: 239 RPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFSRNGKPTIIPKDNEADVGQRYKLS 298

Query: 731 QND 739
           + D
Sbjct: 299 EMD 301
>sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precursor (Nematode astacin 15)
          Length = 571

 Score = 93.2 bits (230), Expect = 7e-19
 Identities = 62/192 (32%), Positives = 97/192 (50%), Gaps = 5/192 (2%)
 Frame = +2

Query: 179 NGFKNHYANSKKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKF--QESTNGH 352
           N  KN     + WP   + Y+ +  +       +  S+ E  +    C+++  +E+ + +
Sbjct: 114 NAIKNRL---QLWPEGRIPYTISSQYSSYSRSLIAASMQEYASHT--CIRWVPKEAADVN 168

Query: 353 YIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQ 532
           Y+ +   + GCYS++G  GG  Q L+L S GC+  KG I HE MHA+GF H   R DRD 
Sbjct: 169 YVHIYPDR-GCYSMVGKMGG-KQSLSLGS-GCI-QKGIILHELMHAVGFFHEQSRTDRDD 224

Query: 533 HISINWARILSSQCSQFVKCDGCEPDG---PYETESVMHYNSEGFACNPGEKVVLKKNGG 703
           HI+I W  I +    QF K            Y+  S+MHY ++ F+ N    ++ KKNG 
Sbjct: 225 HITIMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHYGTKAFSRNGQPTMIPKKNGA 284

Query: 704 AIGYNHGTTQND 739
            IG  +G ++ D
Sbjct: 285 TIGQRNGFSKVD 296
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
          Length = 251

 Score = 92.8 bits (229), Expect = 1e-18
 Identities = 57/166 (34%), Positives = 89/166 (53%), Gaps = 4/166 (2%)
 Frame = +2

Query: 215 WPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKF-QESTNGHYIEVNSKQEGCYS 391
           W   V+ Y+FAG    D+  A+   + EL+   + C++F   +T   Y+E+ +   GC+S
Sbjct: 59  WSGGVIPYTFAGVSGADQ-SAILSGMQELEE--KTCIRFVPRTTESDYVEIFTSGSGCWS 115

Query: 392 LLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILSSQ 571
            +G   G  Q ++L++ GC+Y  GTI HE MHA+GF H H R DRD +++IN+  +  S 
Sbjct: 116 YVGRISGAQQ-VSLQANGCVYH-GTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSM 173

Query: 572 CSQF-VKCDGCEPDGPYETESVMHYNSEGFACNPG--EKVVLKKNG 700
            S F +          Y+  S+MHY    F+   G  E +V  +NG
Sbjct: 174 TSNFDIDTYSRYVGEDYQYYSIMHYGKYSFSIQWGVLETIVPLQNG 219
>sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatching enzyme zinc-protease LCE
           subunit) (Choriolysin L)
          Length = 271

 Score = 89.7 bits (221), Expect = 8e-18
 Identities = 64/223 (28%), Positives = 100/223 (44%), Gaps = 7/223 (3%)
 Frame = +2

Query: 104 KLLKRSLSTVKLGDMLLDSTDIAIINGFKNHYA-NSKKWPNNV-----VLYSFAGNFPRD 265
           ++   S+  +  GD++L  T     N  K   A +S +WP +      V Y  + N+  D
Sbjct: 51  RMNNNSMEELLEGDLVLPKTR----NAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESD 106

Query: 266 KIGAVRESLGELQNDLRGCVKFQESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPG 445
           +   +R ++ E     + C+ F    N         + GC S++G+ G   Q + L+  G
Sbjct: 107 EKETIRNAMKEFAE--KTCIHFVPRNNERAYLSLEPRFGCKSMMGYVGD-KQVVVLQRFG 163

Query: 446 CLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILSSQCSQFVKCDGCEPDGPYET 625
           C+     I+HE +HALGF H H R DRDQH+ INW  I+      F K D      PY+ 
Sbjct: 164 CI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDY 222

Query: 626 ESVMHYNSEGFACNPGEKVV-LKKNGGAIGYNHGTTQNDLTMI 751
            S+MHY    F  +  E +  +     AIG     +  D+  +
Sbjct: 223 GSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRV 265
>sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor (Nematode astacin 6)
          Length = 344

 Score = 87.4 bits (215), Expect = 4e-17
 Identities = 59/180 (32%), Positives = 87/180 (48%), Gaps = 4/180 (2%)
 Frame = +2

Query: 173 IINGFKNHYANSKKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNG- 349
           + N  KN       W   V+ Y     F  ++I  + ++    +     C++F++     
Sbjct: 70  LFNALKNKQLT---WEGGVIPYEMDTAFSPNEIKILEKAFDSYRRTT--CIRFEKREGQT 124

Query: 350 HYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRD 529
            Y+ +  K  GCYS +G TGG  Q ++L   GC + +  I HE MH++GF H H R DRD
Sbjct: 125 DYLNI-VKGYGCYSQVGRTGG-KQEISL-GRGCFFHE-IIVHELMHSVGFWHEHSRADRD 180

Query: 530 QHISINWARILSSQCSQFVKCDGCEPD---GPYETESVMHYNSEGFACNPGEKVVLKKNG 700
            HI INW  IL    SQF K      D     Y+ +S+MHY+S  F+ N    +   +NG
Sbjct: 181 DHIKINWDNILPGMKSQFDKISAVLQDLQGENYDYKSIMHYDSTAFSRNGRNTIETVENG 240
>sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor (Nematode astacin 8)
          Length = 403

 Score = 87.0 bits (214), Expect = 5e-17
 Identities = 62/169 (36%), Positives = 91/169 (53%), Gaps = 5/169 (2%)
 Frame = +2

Query: 206 SKKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQEST--NGHYIEVNSKQE 379
           ++KWPN  + Y  +  +  D+  AV     +  +D + CV+F   T  +  Y+ +  K +
Sbjct: 118 TRKWPNGRIPYVISNQY-NDRERAVLARSFQAYHD-KTCVRFVPRTAVDNDYLYIG-KID 174

Query: 380 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARI 559
           GCYS +G  GG  Q L+L++ GCL     I HE MH++GF H H R DRD+HI+I W  I
Sbjct: 175 GCYSDVGRAGG-RQELSLDN-GCLQYDTAI-HELMHSVGFYHEHERWDRDEHITILWHNI 231

Query: 560 LSSQCSQFVKCDGCEPD---GPYETESVMHYNSEGFACNPGEKVVLKKN 697
                 QF K D  E       Y+  S+MHY+S  F+ N  E +V K++
Sbjct: 232 DREAYDQFGKVDLAESSYYGQLYDYYSIMHYDSLAFSKNGFETMVAKQS 280
>sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precursor (Nematode astacin 13)
          Length = 527

 Score = 86.3 bits (212), Expect = 9e-17
 Identities = 59/185 (31%), Positives = 90/185 (48%), Gaps = 5/185 (2%)
 Frame = +2

Query: 212 KWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH--YIEVNSKQEGC 385
           KW    + Y+ +  +       + E++ E +   + C+ F   + G   YI +    +GC
Sbjct: 195 KWEQARIPYTISSQYSSYSRSKIAEAIEEYRK--KTCIDFSPKSAGDLDYIHI-VPDDGC 251

Query: 386 YSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILS 565
           YSL+G  GG  QP++L   GC+  KG I HE MHA+GF H   R DRD+++ INW+ + +
Sbjct: 252 YSLVGRIGG-KQPVSL-GDGCI-QKGIIIHELMHAVGFFHEQSRADRDEYVKINWSNVEA 308

Query: 566 SQCSQFVKCDGCEPD---GPYETESVMHYNSEGFACNPGEKVVLKKNGGAIGYNHGTTQN 736
               QF K      D     Y+  SVMHY    F+ N    +   +    IG   G ++N
Sbjct: 309 GLQDQFDKYSLNMIDHLGTKYDYGSVMHYAPTAFSKNGKPTIEPIEKNVEIGQRAGFSEN 368

Query: 737 DLTMI 751
           D+  I
Sbjct: 369 DIYKI 373
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,517,632
Number of Sequences: 369166
Number of extensions: 2103239
Number of successful extensions: 5268
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5003
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5203
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7618580670
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00359

  1. Dr_sW_009_O11
  2. Dr_sW_025_D02
  3. Dr_sW_022_E04
  4. Dr_sW_009_I05