Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_025_D02
(564 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor... 87 4e-17
sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-mole... 86 8e-17
sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (... 84 2e-16
sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (... 80 5e-15
sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatch... 77 3e-14
sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precurs... 74 2e-13
sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precurs... 74 2e-13
sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor... 73 6e-13
sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor... 72 7e-13
sp|Q21178|NAS17_CAEEL Zinc metalloproteinase nas-17 precurs... 72 7e-13
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
Length = 315
Score = 86.7 bits (213), Expect = 4e-17
Identities = 55/158 (34%), Positives = 84/158 (53%), Gaps = 10/158 (6%)
Frame = +1
Query: 79 GDMLLDSTDIAII-------NGFKNHYANSKKWPNNVVLYSFAGNFPRDKIGAVRESLGE 237
GD+LL+S + N K Y ++WPNN + Y+ + + + ++ E
Sbjct: 75 GDILLESPKKFVEENNKLGRNAIKQIY---RRWPNNEIPYTLSSQYGSYARSVIANAMNE 131
Query: 238 LQNDLRGCVKF---QESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTI 408
+ CVKF S + Y+ ++ EGCYSL+G TGG QP++L+S GC+ GTI
Sbjct: 132 YHT--KTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG-KQPVSLDS-GCI-QVGTI 185
Query: 409 KHEFMHALGFMHTHMRKDRDQHISINWARILSSQCSQF 522
HE MHA+GF H R+DRD +I + W +++ QF
Sbjct: 186 VHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQF 223
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
Length = 251
Score = 85.5 bits (210), Expect = 8e-17
Identities = 46/124 (37%), Positives = 72/124 (58%), Gaps = 1/124 (0%)
Frame = +1
Query: 154 WPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKF-QESTNGHYIEVNSKQEGCYS 330
W V+ Y+FAG D+ A+ + EL+ + C++F +T Y+E+ + GC+S
Sbjct: 59 WSGGVIPYTFAGVSGADQ-SAILSGMQELEE--KTCIRFVPRTTESDYVEIFTSGSGCWS 115
Query: 331 LLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILSSQ 510
+G G Q ++L++ GC+Y GTI HE MHA+GF H H R DRD +++IN+ + S
Sbjct: 116 YVGRISGAQQ-VSLQANGCVYH-GTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSM 173
Query: 511 CSQF 522
S F
Sbjct: 174 TSNF 177
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
HCE 2 subunit) (Choriolysin H 2)
Length = 279
Score = 84.3 bits (207), Expect = 2e-16
Identities = 49/133 (36%), Positives = 71/133 (53%), Gaps = 1/133 (0%)
Frame = +1
Query: 166 VVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH-YIEVNSKQEGCYSLLGF 342
V+ Y + + R ++ + ++ R C++F TN + +I V SK GCYS LG
Sbjct: 100 VIPYVISSQYSRGEVATIEGAMRAFNG--RTCIRFVRRTNEYDFISVVSKN-GCYSELGR 156
Query: 343 TGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILSSQCSQF 522
GG Q L+L GC+YS G I+HE HALGF H R DRD ++ INW I+ + F
Sbjct: 157 KGG-QQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNF 214
Query: 523 VRCDGCEPDGPYE 561
+ D + PY+
Sbjct: 215 NKHDTNNLNTPYD 227
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
HCE 1 subunit) (Choriolysin H 1)
Length = 270
Score = 79.7 bits (195), Expect = 5e-15
Identities = 49/141 (34%), Positives = 74/141 (52%), Gaps = 3/141 (2%)
Frame = +1
Query: 148 KKWPNNVVL--YSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH-YIEVNSKQE 318
KK N +V+ Y + + ++ + ++ + C++F TN + +I V SK
Sbjct: 83 KKASNGLVVIPYVISSEYSGGEVATIEGAMRAFNG--KTCIRFVRRTNEYDFISVVSKT- 139
Query: 319 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARI 498
GCYS LG GG Q L++ GC+YS G I+HE HALGF H R DRD ++ INW I
Sbjct: 140 GCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENI 197
Query: 499 LSSQCSQFVRCDGCEPDGPYE 561
+ + F + D + PY+
Sbjct: 198 IPASAYNFNKHDTNNLNTPYD 218
>sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatching enzyme zinc-protease LCE
subunit) (Choriolysin L)
Length = 271
Score = 77.0 bits (188), Expect = 3e-14
Identities = 53/179 (29%), Positives = 83/179 (46%), Gaps = 6/179 (3%)
Frame = +1
Query: 43 KLLKRSLSTVKLGDMLLDSTDIAIINGFKNHYA-NSKKWPNNV-----VLYSFAGNFPRD 204
++ S+ + GD++L T N K A +S +WP + V Y + N+ D
Sbjct: 51 RMNNNSMEELLEGDLVLPKTR----NAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESD 106
Query: 205 KIGAVRESLGELQNDLRGCVKFQESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPG 384
+ +R ++ E + C+ F N + GC S++G+ G Q + L+ G
Sbjct: 107 EKETIRNAMKEFAE--KTCIHFVPRNNERAYLSLEPRFGCKSMMGYVGD-KQVVVLQRFG 163
Query: 385 CLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILSSQCSQFVRCDGCEPDGPYE 561
C+ I+HE +HALGF H H R DRDQH+ INW I+ F + D PY+
Sbjct: 164 CI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYD 221
>sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precursor (Nematode astacin 13)
Length = 527
Score = 74.3 bits (181), Expect = 2e-13
Identities = 43/126 (34%), Positives = 67/126 (53%), Gaps = 2/126 (1%)
Frame = +1
Query: 151 KWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH--YIEVNSKQEGC 324
KW + Y+ + + + E++ E + + C+ F + G YI + +GC
Sbjct: 195 KWEQARIPYTISSQYSSYSRSKIAEAIEEYRK--KTCIDFSPKSAGDLDYIHI-VPDDGC 251
Query: 325 YSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILS 504
YSL+G GG QP++L GC+ KG I HE MHA+GF H R DRD+++ INW+ + +
Sbjct: 252 YSLVGRIGG-KQPVSL-GDGCI-QKGIIIHELMHAVGFFHEQSRADRDEYVKINWSNVEA 308
Query: 505 SQCSQF 522
QF
Sbjct: 309 GLQDQF 314
>sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precursor (Nematode astacin 14)
Length = 503
Score = 74.3 bits (181), Expect = 2e-13
Identities = 44/116 (37%), Positives = 60/116 (51%), Gaps = 3/116 (2%)
Frame = +1
Query: 148 KKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGHYIEVNSKQE--- 318
K WP V Y D+ A+ ++ E + + CV+F T+ + + K+
Sbjct: 123 KLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKT--KTCVRFVPKTDDDFDYIYVKRNVAF 180
Query: 319 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISIN 486
GC S +G GG NQ ++LE C +SKG I HE MHALGF H H R DRD + IN
Sbjct: 181 GCSSYVGRAGG-NQTVSLEVDKC-FSKGIIAHELMHALGFFHEHSRTDRDDFVDIN 234
>sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor (Nematode astacin 8)
Length = 403
Score = 72.8 bits (177), Expect = 6e-13
Identities = 50/135 (37%), Positives = 73/135 (54%), Gaps = 2/135 (1%)
Frame = +1
Query: 145 SKKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQEST--NGHYIEVNSKQE 318
++KWPN + Y + + D+ AV + +D + CV+F T + Y+ + K +
Sbjct: 118 TRKWPNGRIPYVISNQY-NDRERAVLARSFQAYHD-KTCVRFVPRTAVDNDYLYIG-KID 174
Query: 319 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARI 498
GCYS +G GG Q L+L++ GCL I HE MH++GF H H R DRD+HI+I W I
Sbjct: 175 GCYSDVGRAGG-RQELSLDN-GCLQYDTAI-HELMHSVGFYHEHERWDRDEHITILWHNI 231
Query: 499 LSSQCSQFVRCDGCE 543
QF + D E
Sbjct: 232 DREAYDQFGKVDLAE 246
>sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor (Nematode astacin 6)
Length = 344
Score = 72.4 bits (176), Expect = 7e-13
Identities = 47/138 (34%), Positives = 68/138 (49%), Gaps = 1/138 (0%)
Frame = +1
Query: 112 IINGFKNHYANSKKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNG- 288
+ N KN W V+ Y F ++I + ++ + C++F++
Sbjct: 70 LFNALKNKQLT---WEGGVIPYEMDTAFSPNEIKILEKAFDSYRRTT--CIRFEKREGQT 124
Query: 289 HYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRD 468
Y+ + K GCYS +G TGG Q ++L GC + + I HE MH++GF H H R DRD
Sbjct: 125 DYLNI-VKGYGCYSQVGRTGG-KQEISL-GRGCFFHE-IIVHELMHSVGFWHEHSRADRD 180
Query: 469 QHISINWARILSSQCSQF 522
HI INW IL SQF
Sbjct: 181 DHIKINWDNILPGMKSQF 198
>sp|Q21178|NAS17_CAEEL Zinc metalloproteinase nas-17 precursor (Nematode astacin 17)
Length = 429
Score = 72.4 bits (176), Expect = 7e-13
Identities = 48/142 (33%), Positives = 68/142 (47%), Gaps = 5/142 (3%)
Frame = +1
Query: 79 GDMLLDSTDIAIINGFKNHYANS-----KKWPNNVVLYSFAGNFPRDKIGAVRESLGELQ 243
GD++L + I+NG KKWP+ V Y + F K + ++ +
Sbjct: 41 GDIMLTEAQLRILNGTAKRSKRQITKIWKKWPDAKVFYYYENEFTSLKRELMSYAMAHIS 100
Query: 244 NDLRGCVKFQESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFM 423
++ CVKFQES + + GC S +G GG Q L GCL GT HE M
Sbjct: 101 SNT--CVKFQESNSATNRIRFTNTGGCASYIGMNGG-EQTLWF-GDGCLIF-GTAVHEIM 155
Query: 424 HALGFMHTHMRKDRDQHISINW 489
H+LG HTH R DRD +S+++
Sbjct: 156 HSLGLFHTHSRFDRDNFLSVSY 177
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,052,672
Number of Sequences: 369166
Number of extensions: 1455328
Number of successful extensions: 3663
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3531
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3636
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4078830820
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)