Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_009_I05
(804 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor... 102 1e-21
sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (... 101 3e-21
sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (... 98 3e-20
sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precurs... 95 3e-19
sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precurs... 93 7e-19
sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-mole... 93 1e-18
sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatch... 90 8e-18
sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor... 87 4e-17
sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor... 87 5e-17
sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precurs... 86 9e-17
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
Length = 315
Score = 102 bits (254), Expect = 1e-21
Identities = 68/200 (34%), Positives = 105/200 (52%), Gaps = 13/200 (6%)
Frame = +2
Query: 140 GDMLLDSTDIAII-------NGFKNHYANSKKWPNNVVLYSFAGNFPRDKIGAVRESLGE 298
GD+LL+S + N K Y ++WPNN + Y+ + + + ++ E
Sbjct: 75 GDILLESPKKFVEENNKLGRNAIKQIY---RRWPNNEIPYTLSSQYGSYARSVIANAMNE 131
Query: 299 LQNDLRGCVKF---QESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTI 469
+ CVKF S + Y+ ++ EGCYSL+G TGG QP++L+S GC+ GTI
Sbjct: 132 YHT--KTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG-KQPVSLDS-GCI-QVGTI 185
Query: 470 KHEFMHALGFMHTHMRKDRDQHISINWARILSSQCSQFVKCD---GCEPDGPYETESVMH 640
HE MHA+GF H R+DRD +I + W +++ QF K + D PY+ S+MH
Sbjct: 186 VHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMH 245
Query: 641 YNSEGFACNPGEKVVLKKNG 700
Y F+ + + +V KK+G
Sbjct: 246 YGPYAFSGSGKKTLVPKKSG 265
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
HCE 2 subunit) (Choriolysin H 2)
Length = 279
Score = 101 bits (251), Expect = 3e-21
Identities = 61/176 (34%), Positives = 91/176 (51%), Gaps = 3/176 (1%)
Frame = +2
Query: 227 VVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH-YIEVNSKQEGCYSLLGF 403
V+ Y + + R ++ + ++ R C++F TN + +I V SK GCYS LG
Sbjct: 100 VIPYVISSQYSRGEVATIEGAMRAFNG--RTCIRFVRRTNEYDFISVVSKN-GCYSELGR 156
Query: 404 TGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILSSQCSQF 583
GG Q L+L GC+YS G I+HE HALGF H R DRD ++ INW I+ + F
Sbjct: 157 KGG-QQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNF 214
Query: 584 VKCDGCEPDGPYETESVMHYNSEGFACNPGEKVV--LKKNGGAIGYNHGTTQNDLT 745
K D + PY+ S+MHY + F+ G + + IG +G ++ D+T
Sbjct: 215 NKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSRWDIT 270
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
HCE 1 subunit) (Choriolysin H 1)
Length = 270
Score = 97.8 bits (242), Expect = 3e-20
Identities = 62/186 (33%), Positives = 95/186 (51%), Gaps = 5/186 (2%)
Frame = +2
Query: 209 KKWPNNVVL--YSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH-YIEVNSKQE 379
KK N +V+ Y + + ++ + ++ + C++F TN + +I V SK
Sbjct: 83 KKASNGLVVIPYVISSEYSGGEVATIEGAMRAFNG--KTCIRFVRRTNEYDFISVVSKT- 139
Query: 380 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARI 559
GCYS LG GG Q L++ GC+YS G I+HE HALGF H R DRD ++ INW I
Sbjct: 140 GCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENI 197
Query: 560 LSSQCSQFVKCDGCEPDGPYETESVMHYNSEGFACNPGEKVV--LKKNGGAIGYNHGTTQ 733
+ + F K D + PY+ S+MHY + F+ G + + IG +G ++
Sbjct: 198 IPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGRDSITPIPNPNVPIGQRNGMSR 257
Query: 734 NDLTMI 751
D+T I
Sbjct: 258 WDITRI 263
>sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precursor (Nematode astacin 14)
Length = 503
Score = 94.7 bits (234), Expect = 3e-19
Identities = 62/183 (33%), Positives = 87/183 (47%), Gaps = 6/183 (3%)
Frame = +2
Query: 209 KKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGHYIEVNSKQE--- 379
K WP V Y D+ A+ ++ E + + CV+F T+ + + K+
Sbjct: 123 KLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKT--KTCVRFVPKTDDDFDYIYVKRNVAF 180
Query: 380 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARI 559
GC S +G GG NQ ++LE C +SKG I HE MHALGF H H R DRD + IN I
Sbjct: 181 GCSSYVGRAGG-NQTVSLEVDKC-FSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNI 238
Query: 560 LSSQCSQFVKCDGCEPDG---PYETESVMHYNSEGFACNPGEKVVLKKNGGAIGYNHGTT 730
F K D PY+ ESVMHY+ F+ N ++ K N +G + +
Sbjct: 239 RPGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFSRNGKPTIIPKDNEADVGQRYKLS 298
Query: 731 QND 739
+ D
Sbjct: 299 EMD 301
>sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precursor (Nematode astacin 15)
Length = 571
Score = 93.2 bits (230), Expect = 7e-19
Identities = 62/192 (32%), Positives = 97/192 (50%), Gaps = 5/192 (2%)
Frame = +2
Query: 179 NGFKNHYANSKKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKF--QESTNGH 352
N KN + WP + Y+ + + + S+ E + C+++ +E+ + +
Sbjct: 114 NAIKNRL---QLWPEGRIPYTISSQYSSYSRSLIAASMQEYASHT--CIRWVPKEAADVN 168
Query: 353 YIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQ 532
Y+ + + GCYS++G GG Q L+L S GC+ KG I HE MHA+GF H R DRD
Sbjct: 169 YVHIYPDR-GCYSMVGKMGG-KQSLSLGS-GCI-QKGIILHELMHAVGFFHEQSRTDRDD 224
Query: 533 HISINWARILSSQCSQFVKCDGCEPDG---PYETESVMHYNSEGFACNPGEKVVLKKNGG 703
HI+I W I + QF K Y+ S+MHY ++ F+ N ++ KKNG
Sbjct: 225 HITIMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHYGTKAFSRNGQPTMIPKKNGA 284
Query: 704 AIGYNHGTTQND 739
IG +G ++ D
Sbjct: 285 TIGQRNGFSKVD 296
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
Length = 251
Score = 92.8 bits (229), Expect = 1e-18
Identities = 57/166 (34%), Positives = 89/166 (53%), Gaps = 4/166 (2%)
Frame = +2
Query: 215 WPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKF-QESTNGHYIEVNSKQEGCYS 391
W V+ Y+FAG D+ A+ + EL+ + C++F +T Y+E+ + GC+S
Sbjct: 59 WSGGVIPYTFAGVSGADQ-SAILSGMQELEE--KTCIRFVPRTTESDYVEIFTSGSGCWS 115
Query: 392 LLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILSSQ 571
+G G Q ++L++ GC+Y GTI HE MHA+GF H H R DRD +++IN+ + S
Sbjct: 116 YVGRISGAQQ-VSLQANGCVYH-GTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSM 173
Query: 572 CSQF-VKCDGCEPDGPYETESVMHYNSEGFACNPG--EKVVLKKNG 700
S F + Y+ S+MHY F+ G E +V +NG
Sbjct: 174 TSNFDIDTYSRYVGEDYQYYSIMHYGKYSFSIQWGVLETIVPLQNG 219
>sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatching enzyme zinc-protease LCE
subunit) (Choriolysin L)
Length = 271
Score = 89.7 bits (221), Expect = 8e-18
Identities = 64/223 (28%), Positives = 100/223 (44%), Gaps = 7/223 (3%)
Frame = +2
Query: 104 KLLKRSLSTVKLGDMLLDSTDIAIINGFKNHYA-NSKKWPNNV-----VLYSFAGNFPRD 265
++ S+ + GD++L T N K A +S +WP + V Y + N+ D
Sbjct: 51 RMNNNSMEELLEGDLVLPKTR----NAMKCFGAPDSCRWPKSSNGIVKVPYVVSDNYESD 106
Query: 266 KIGAVRESLGELQNDLRGCVKFQESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPG 445
+ +R ++ E + C+ F N + GC S++G+ G Q + L+ G
Sbjct: 107 EKETIRNAMKEFAE--KTCIHFVPRNNERAYLSLEPRFGCKSMMGYVGD-KQVVVLQRFG 163
Query: 446 CLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILSSQCSQFVKCDGCEPDGPYET 625
C+ I+HE +HALGF H H R DRDQH+ INW I+ F K D PY+
Sbjct: 164 CI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLGTPYDY 222
Query: 626 ESVMHYNSEGFACNPGEKVV-LKKNGGAIGYNHGTTQNDLTMI 751
S+MHY F + E + + AIG + D+ +
Sbjct: 223 GSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRV 265
>sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor (Nematode astacin 6)
Length = 344
Score = 87.4 bits (215), Expect = 4e-17
Identities = 59/180 (32%), Positives = 87/180 (48%), Gaps = 4/180 (2%)
Frame = +2
Query: 173 IINGFKNHYANSKKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNG- 349
+ N KN W V+ Y F ++I + ++ + C++F++
Sbjct: 70 LFNALKNKQLT---WEGGVIPYEMDTAFSPNEIKILEKAFDSYRRTT--CIRFEKREGQT 124
Query: 350 HYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRD 529
Y+ + K GCYS +G TGG Q ++L GC + + I HE MH++GF H H R DRD
Sbjct: 125 DYLNI-VKGYGCYSQVGRTGG-KQEISL-GRGCFFHE-IIVHELMHSVGFWHEHSRADRD 180
Query: 530 QHISINWARILSSQCSQFVKCDGCEPD---GPYETESVMHYNSEGFACNPGEKVVLKKNG 700
HI INW IL SQF K D Y+ +S+MHY+S F+ N + +NG
Sbjct: 181 DHIKINWDNILPGMKSQFDKISAVLQDLQGENYDYKSIMHYDSTAFSRNGRNTIETVENG 240
>sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor (Nematode astacin 8)
Length = 403
Score = 87.0 bits (214), Expect = 5e-17
Identities = 62/169 (36%), Positives = 91/169 (53%), Gaps = 5/169 (2%)
Frame = +2
Query: 206 SKKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQEST--NGHYIEVNSKQE 379
++KWPN + Y + + D+ AV + +D + CV+F T + Y+ + K +
Sbjct: 118 TRKWPNGRIPYVISNQY-NDRERAVLARSFQAYHD-KTCVRFVPRTAVDNDYLYIG-KID 174
Query: 380 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARI 559
GCYS +G GG Q L+L++ GCL I HE MH++GF H H R DRD+HI+I W I
Sbjct: 175 GCYSDVGRAGG-RQELSLDN-GCLQYDTAI-HELMHSVGFYHEHERWDRDEHITILWHNI 231
Query: 560 LSSQCSQFVKCDGCEPD---GPYETESVMHYNSEGFACNPGEKVVLKKN 697
QF K D E Y+ S+MHY+S F+ N E +V K++
Sbjct: 232 DREAYDQFGKVDLAESSYYGQLYDYYSIMHYDSLAFSKNGFETMVAKQS 280
>sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precursor (Nematode astacin 13)
Length = 527
Score = 86.3 bits (212), Expect = 9e-17
Identities = 59/185 (31%), Positives = 90/185 (48%), Gaps = 5/185 (2%)
Frame = +2
Query: 212 KWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH--YIEVNSKQEGC 385
KW + Y+ + + + E++ E + + C+ F + G YI + +GC
Sbjct: 195 KWEQARIPYTISSQYSSYSRSKIAEAIEEYRK--KTCIDFSPKSAGDLDYIHI-VPDDGC 251
Query: 386 YSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKDRDQHISINWARILS 565
YSL+G GG QP++L GC+ KG I HE MHA+GF H R DRD+++ INW+ + +
Sbjct: 252 YSLVGRIGG-KQPVSL-GDGCI-QKGIIIHELMHAVGFFHEQSRADRDEYVKINWSNVEA 308
Query: 566 SQCSQFVKCDGCEPD---GPYETESVMHYNSEGFACNPGEKVVLKKNGGAIGYNHGTTQN 736
QF K D Y+ SVMHY F+ N + + IG G ++N
Sbjct: 309 GLQDQFDKYSLNMIDHLGTKYDYGSVMHYAPTAFSKNGKPTIEPIEKNVEIGQRAGFSEN 368
Query: 737 DLTMI 751
D+ I
Sbjct: 369 DIYKI 373
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,517,632
Number of Sequences: 369166
Number of extensions: 2103239
Number of successful extensions: 5268
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5003
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5203
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7618580670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)