Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_022_E04
(519 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor... 70 3e-12
sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-mole... 69 9e-12
sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precurs... 64 3e-10
sp|Q21178|NAS17_CAEEL Zinc metalloproteinase nas-17 precurs... 63 5e-10
sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (... 62 8e-10
sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (... 57 2e-08
sp|Q22396|NAS20_CAEEL Zinc metalloproteinase nas-20 precurs... 57 3e-08
sp|Q21252|NAS3_CAEEL Zinc metalloproteinase nas-3 precursor... 57 3e-08
sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precurs... 54 2e-07
sp|O16977|NAS32_CAEEL Zinc metalloproteinase nas-32 precurs... 53 4e-07
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
Length = 315
Score = 70.1 bits (170), Expect = 3e-12
Identities = 49/143 (34%), Positives = 74/143 (51%), Gaps = 10/143 (6%)
Frame = +3
Query: 117 GDMLLDSTDIAII-------NGFKNHYANSKKWPNNVVLYSFAGNFPRDKIGAVRESLGE 275
GD+LL+S + N K Y ++WPNN + Y+ + + + ++ E
Sbjct: 75 GDILLESPKKFVEENNKLGRNAIKQIY---RRWPNNEIPYTLSSQYGSYARSVIANAMNE 131
Query: 276 LQNDLRGCVKF---QESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTI 446
+ CVKF S + Y+ ++ EGCYSL+G TGG QP++L+S GC+ GTI
Sbjct: 132 YHT--KTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG-KQPVSLDS-GCI-QVGTI 185
Query: 447 KHEFMHALGFMHTHMRKTETSIL 515
HE MHA+GF H R+ S +
Sbjct: 186 VHELMHAVGFFHEQSRQDRDSYI 208
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
Length = 251
Score = 68.6 bits (166), Expect = 9e-12
Identities = 38/102 (37%), Positives = 59/102 (57%), Gaps = 1/102 (0%)
Frame = +3
Query: 192 WPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKF-QESTNGHYIEVNSKQEGCYS 368
W V+ Y+FAG D+ A+ + EL+ + C++F +T Y+E+ + GC+S
Sbjct: 59 WSGGVIPYTFAGVSGADQ-SAILSGMQELEE--KTCIRFVPRTTESDYVEIFTSGSGCWS 115
Query: 369 LLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMR 494
+G G Q ++L++ GC+Y GTI HE MHA+GF H H R
Sbjct: 116 YVGRISGAQQ-VSLQANGCVYH-GTIIHELMHAIGFYHEHTR 155
>sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precursor (Nematode astacin 14)
Length = 503
Score = 63.5 bits (153), Expect = 3e-10
Identities = 39/106 (36%), Positives = 54/106 (50%), Gaps = 3/106 (2%)
Frame = +3
Query: 186 KKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGHYIEVNSKQE--- 356
K WP V Y D+ A+ ++ E + + CV+F T+ + + K+
Sbjct: 123 KLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKT--KTCVRFVPKTDDDFDYIYVKRNVAF 180
Query: 357 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMR 494
GC S +G GG NQ ++LE C +SKG I HE MHALGF H H R
Sbjct: 181 GCSSYVGRAGG-NQTVSLEVDKC-FSKGIIAHELMHALGFFHEHSR 224
>sp|Q21178|NAS17_CAEEL Zinc metalloproteinase nas-17 precursor (Nematode astacin 17)
Length = 429
Score = 62.8 bits (151), Expect = 5e-10
Identities = 44/131 (33%), Positives = 60/131 (45%), Gaps = 5/131 (3%)
Frame = +3
Query: 117 GDMLLDSTDIAIINGFKNHYANS-----KKWPNNVVLYSFAGNFPRDKIGAVRESLGELQ 281
GD++L + I+NG KKWP+ V Y + F K + ++ +
Sbjct: 41 GDIMLTEAQLRILNGTAKRSKRQITKIWKKWPDAKVFYYYENEFTSLKRELMSYAMAHIS 100
Query: 282 NDLRGCVKFQESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFM 461
++ CVKFQES + + GC S +G GG Q L GCL GT HE M
Sbjct: 101 SNT--CVKFQESNSATNRIRFTNTGGCASYIGMNGG-EQTLWF-GDGCLIF-GTAVHEIM 155
Query: 462 HALGFMHTHMR 494
H+LG HTH R
Sbjct: 156 HSLGLFHTHSR 166
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
HCE 2 subunit) (Choriolysin H 2)
Length = 279
Score = 62.0 bits (149), Expect = 8e-10
Identities = 39/103 (37%), Positives = 54/103 (52%), Gaps = 1/103 (0%)
Frame = +3
Query: 204 VVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH-YIEVNSKQEGCYSLLGF 380
V+ Y + + R ++ + ++ R C++F TN + +I V SK GCYS LG
Sbjct: 100 VIPYVISSQYSRGEVATIEGAMRAFNG--RTCIRFVRRTNEYDFISVVSKN-GCYSELGR 156
Query: 381 TGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKTETS 509
GG Q L+L GC+YS G I+HE HALGF H R S
Sbjct: 157 KGG-QQELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDS 197
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
HCE 1 subunit) (Choriolysin H 1)
Length = 270
Score = 57.4 bits (137), Expect = 2e-08
Identities = 39/111 (35%), Positives = 57/111 (51%), Gaps = 3/111 (2%)
Frame = +3
Query: 186 KKWPNNVVL--YSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH-YIEVNSKQE 356
KK N +V+ Y + + ++ + ++ + C++F TN + +I V SK
Sbjct: 83 KKASNGLVVIPYVISSEYSGGEVATIEGAMRAFNG--KTCIRFVRRTNEYDFISVVSKT- 139
Query: 357 GCYSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKTETS 509
GCYS LG GG Q L++ GC+YS G I+HE HALGF H R S
Sbjct: 140 GCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDS 188
>sp|Q22396|NAS20_CAEEL Zinc metalloproteinase nas-20 precursor (Nematode astacin 20)
Length = 379
Score = 57.0 bits (136), Expect = 3e-08
Identities = 39/110 (35%), Positives = 50/110 (45%)
Frame = +3
Query: 189 KWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGHYIEVNSKQEGCYS 368
KW NN + F N P + R+++ L+N C+KF+ + N K GCYS
Sbjct: 37 KWENNKMSLFFY-NLPLEMQAMFRDAINYLENHT--CLKFEYNENAETAVRIRKGNGCYS 93
Query: 369 LLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMRKTETSILV 518
L G G Q L L+ C S GT HE MHALG H R L+
Sbjct: 94 LYGMHAGEVQDLTLDY-NCA-SFGTAVHEIMHALGIAHGQARSDRDDYLI 141
>sp|Q21252|NAS3_CAEEL Zinc metalloproteinase nas-3 precursor (Nematode astacin 3)
Length = 292
Score = 56.6 bits (135), Expect = 3e-08
Identities = 39/119 (32%), Positives = 57/119 (47%), Gaps = 15/119 (12%)
Frame = +3
Query: 183 SKKWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQ--ESTNGHYIEVNS--K 350
S WPN V Y A ++ + G + ++ E D+ CV+F+ ST+ HY+++N +
Sbjct: 67 SHLWPNAEVPYDIASHYTATERGIILSAM-EAFRDVT-CVRFRPRRSTDKHYLQINKHYQ 124
Query: 351 QEGCYS---------LLGFTGGPNQPLNLESPGCLY--SKGTIKHEFMHALGFMHTHMR 494
E C+S L G G + P CL +GT+ HE MH LGF H H R
Sbjct: 125 LERCFSYIGRQSSRWLFGTRDGKVETRMKLDPSCLLYNGRGTVMHELMHILGFYHEHQR 183
>sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precursor (Nematode astacin 13)
Length = 527
Score = 54.3 bits (129), Expect = 2e-07
Identities = 35/104 (33%), Positives = 53/104 (50%), Gaps = 2/104 (1%)
Frame = +3
Query: 189 KWPNNVVLYSFAGNFPRDKIGAVRESLGELQNDLRGCVKFQESTNGH--YIEVNSKQEGC 362
KW + Y+ + + + E++ E + + C+ F + G YI + +GC
Sbjct: 195 KWEQARIPYTISSQYSSYSRSKIAEAIEEYRK--KTCIDFSPKSAGDLDYIHI-VPDDGC 251
Query: 363 YSLLGFTGGPNQPLNLESPGCLYSKGTIKHEFMHALGFMHTHMR 494
YSL+G GG QP++L GC+ KG I HE MHA+GF H R
Sbjct: 252 YSLVGRIGG-KQPVSL-GDGCI-QKGIIIHELMHAVGFFHEQSR 292
>sp|O16977|NAS32_CAEEL Zinc metalloproteinase nas-32 precursor (Nematode astacin 32)
Length = 651
Score = 53.1 bits (126), Expect = 4e-07
Identities = 46/138 (33%), Positives = 65/138 (47%), Gaps = 12/138 (8%)
Frame = +3
Query: 117 GDMLLDSTDIAIINGFKNHYANSKK---------WPNNVVLYSFAGNFPRDKIGAVRESL 269
GD+ L++ IA I+ ++ + KK WP VV Y F VR+++
Sbjct: 178 GDINLNNNQIAKISSEQSSKSRRKKRQIDNLAQFWPGKVVYYYFDSGLTTTVQQIVRDAI 237
Query: 270 GELQNDLRGCVKFQ---ESTNGHYIEVNSKQEGCYSLLGFTGGPNQPLNLESPGCLYSKG 440
L+++ C+KF+ +TN + V GCYS G GG Q L+L GC + G
Sbjct: 238 TFLESNT--CLKFELNSTATNRIFSGV-----GCYSDTGMLGG-EQTLSL-GYGCEVT-G 287
Query: 441 TIKHEFMHALGFMHTHMR 494
T HE H LG HT MR
Sbjct: 288 TAAHEIAHTLGLFHTQMR 305
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,553,684
Number of Sequences: 369166
Number of extensions: 1241128
Number of successful extensions: 3040
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2941
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3015
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3403581975
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)