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Planaria EST Database

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00250
         (1082 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P23731|IFEB_ASCSU  Intermediate filament protein B (IF-B)       81   5e-15
sp|Q19289|IFB1_CAEEL  Intermediate filament protein ifb-1 (I...    79   2e-14
sp|P23730|IFEA_ASCSU  Intermediate filament protein A (IF-A)       78   4e-14
sp|P31732|OV71_ONCVO  Muscle cell intermediate filament prot...    78   4e-14
sp|P90900|IFA4_CAEEL  Intermediate filament protein ifa-4 (I...    75   2e-13
sp|Q17107|AV71_ACAVI  Muscle cell intermediate filament prot...    75   2e-13
sp|O02365|IFA2_CAEEL  Intermediate filament protein ifa-2 (I...    75   4e-13
sp|Q21065|IFA3_CAEEL  Intermediate filament protein ifa-3 (I...    75   4e-13
sp|P90901|IFA1_CAEEL  Intermediate filament protein ifa-1 (I...    73   1e-12
sp|Q01241|NF70_LOLPE  70 kDa neurofilament protein (NF70)          65   3e-10

>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
          Length = 589

 Score = 81.3 bits (199), Expect = 5e-15
 Identities = 49/120 (40%), Positives = 70/120 (58%), Gaps = 4/120 (3%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           S +  F+R+ KGN++I+E D NG FITLEN  + K E +    LKR +D + + IV+TI 
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFITLENTHRSKDENLGEHRLKRKLDNRRE-IVYTIP 523

Query: 798 NKTTLNAGEKITIWARGKKTGKGKELEIEEDG---WGLGENITTSLYGADGEEKAVHYQE 968
             T L AG  + I+AR +         +  DG   WG+G N+ TSL   DG+E+A H Q+
Sbjct: 524 PNTVLKAGRTMKIYARDQGGIHNPPDTLVFDGENTWGIGANVVTSLINKDGDERATHTQK 583

 Score = 38.1 bits (87), Expect = 0.044
 Identities = 27/93 (29%), Positives = 38/93 (40%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           ++  I  +R ++ DLE +NA  E  +  +     + QR  E  L      L         
Sbjct: 341 MRDNIGDLRGKLGDLEAKNALLEKEVQNLNYQLNDDQRQYEAALNDRDATLRRMREECQT 400

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESRV 281
                      K  L+ EIA Y K+LE EESRV
Sbjct: 401 LVAELQALLDTKQMLDAEIAIYRKMLEGEESRV 433

>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
           B1) (IF-B1) (Cel IF B1)
          Length = 589

 Score = 79.3 bits (194), Expect = 2e-14
 Identities = 47/132 (35%), Positives = 74/132 (56%), Gaps = 4/132 (3%)
 Frame = +3

Query: 585 NELSSQEAGAYQSKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNI 761
           N  S++      S +  F+R+ KGN++I+E D NG FI LEN  + K E +    ++R +
Sbjct: 453 NTDSTRSVRGEVSTKTTFQRSAKGNVTISECDPNGKFIKLENSHRNKDENVGEHKIRRKL 512

Query: 762 DGQADIIVFTIQNKTTLNAGEKITIWARGKKTGKGKELEIEEDG---WGLGENITTSLYG 932
           DG+ +I V++I     +  G+ +TI+AR +         +  DG   WG+G N+ TSL  
Sbjct: 513 DGRREI-VYSIPANVVIKPGKNLTIYARDQGGINNPPESLVFDGENTWGIGANVVTSLVN 571

Query: 933 ADGEEKAVHYQE 968
            DGEE+A H Q+
Sbjct: 572 KDGEERATHTQK 583

>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
          Length = 497

 Score = 78.2 bits (191), Expect = 4e-14
 Identities = 47/119 (39%), Positives = 70/119 (58%), Gaps = 4/119 (3%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           + R  F+R+ KGN+SI ET  +G ++ LEN  + K E I    LKR IDG+ + IV+T+ 
Sbjct: 374 ASRTSFQRSAKGNVSIQETAPDGRYVVLENTHRSKEEAIGEWKLKRKIDGKRE-IVYTLP 432

Query: 798 NKTTLNAGEKITIWARGKK--TGKGKELEIE-EDGWGLGENITTSLYGADGEEKAVHYQ 965
               L  G+ + IWARG+       ++L  + ED +G G N+ T L+  +GEE+A H Q
Sbjct: 433 RDFILRPGKSVKIWARGQGGIHSPPEQLVFDLEDTFGSGSNVQTILFNREGEERATHIQ 491

>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
          Length = 432

 Score = 78.2 bits (191), Expect = 4e-14
 Identities = 46/118 (38%), Positives = 67/118 (56%), Gaps = 3/118 (2%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           + R  F+R+ KGN+SI +   +G FI LEN  + K E I    LKR IDG+ +I V+T  
Sbjct: 309 ASRTSFQRSAKGNVSIQDASSDGKFILLENTHRSKEEPIGEWRLKRKIDGKREI-VYTFP 367

Query: 798 NKTTLNAGEKITIWARGKKTGKGKELEI--EEDGWGLGENITTSLYGADGEEKAVHYQ 965
               L  G+ + IWARG+      +  +   ED +G+G N+ T L+  +GEE+A H Q
Sbjct: 368 RDFILKPGKTVKIWARGQGVYSPPDQLVFDAEDSFGVGSNVQTILFNKEGEERASHIQ 425

 Score = 42.4 bits (98), Expect = 0.002
 Identities = 27/92 (29%), Positives = 42/92 (45%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           ++ Q+  +R ++ADLE +N+  E  +  +    E+ QRS E  L     Q+         
Sbjct: 185 LRVQLSDLRGKLADLEGRNSLLEKQMQELNYQLEDDQRSYEAALNDRDAQIRKMREECQA 244

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESR 278
                      K  L+ EIA Y K+LE EE+R
Sbjct: 245 LMMELQMLLDTKQTLDAEIAIYRKMLEGEENR 276

>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
           A4) (IF-A4) (Cel IF A4)
          Length = 575

 Score = 75.5 bits (184), Expect = 2e-13
 Identities = 42/112 (37%), Positives = 67/112 (59%), Gaps = 2/112 (1%)
 Frame = +3

Query: 636 FRRTTKGNISIAETDINGLFITLENIGQKFETITGMTLKRNIDGQADIIVFTIQNKTTLN 815
           + R+ KGNI+I E + +G +I LENI ++ E I    L+R I G+ + IVFT   + TL 
Sbjct: 460 YSRSAKGNIAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAGKRE-IVFTFpreFTLR 518

Query: 816 AGEKITIWARGKKTGKGKELEIE--EDGWGLGENITTSLYGADGEEKAVHYQ 965
           A + + I+ARG+      +  +   ED +G G ++ T+LY  +GE++A H Q
Sbjct: 519 AQKSVKIFARGQGVHSPPDSLVYDLEDSFGTGNDVVTTLYNKEGEDRASHSQ 570

>sp|Q17107|AV71_ACAVI Muscle cell intermediate filament protein AV71
          Length = 394

 Score = 75.5 bits (184), Expect = 2e-13
 Identities = 44/118 (37%), Positives = 66/118 (55%), Gaps = 3/118 (2%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           + R  F+R+ KGN+SI +   +G +I  EN  + K E I    LKR IDG+ +I V+T  
Sbjct: 271 ASRTSFQRSAKGNVSIQDAASDGKYILSENTHRSKEEPIGEWRLKRKIDGKREI-VYTFP 329

Query: 798 NKTTLNAGEKITIWARGKKTGKGKELEI--EEDGWGLGENITTSLYGADGEEKAVHYQ 965
               L  G+ + IWARG+      +  +   ED +G+G N+ T L+  +GEE+A H Q
Sbjct: 330 TNFVLKPGKSVKIWARGQGVYSPPDQLVFDAEDSFGIGSNVQTILFNKEGEERASHIQ 387

 Score = 41.2 bits (95), Expect = 0.005
 Identities = 27/92 (29%), Positives = 41/92 (44%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           ++ Q+  +R ++ADLE +N+  E     +    E+ QRS E  L     Q+         
Sbjct: 147 LRVQLSELRGKLADLEGRNSLLEKQTQELNYQLEDDQRSYEAALNDRDAQIRKMREECQA 206

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESR 278
                      K  L+ EIA Y K+LE EE+R
Sbjct: 207 LMMELQMLLDTKQTLDAEIAIYRKMLEGEENR 238

>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
           A2) (IF-A2) (Cel IF A2)
          Length = 581

 Score = 74.7 bits (182), Expect = 4e-13
 Identities = 46/118 (38%), Positives = 68/118 (57%), Gaps = 3/118 (2%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           S R  ++R+ KGN++I ET   G F+ LEN  + K E +    LKR IDG+ + IVFT  
Sbjct: 456 SSRTSYQRSAKGNVAIKETSPEGKFVILENTHRAKEEPLGDWKLKRKIDGKRE-IVFTFP 514

Query: 798 NKTTLNAGEKITIWARGKKTGKGKELEIEE--DGWGLGENITTSLYGADGEEKAVHYQ 965
           +   L+  + + I+ARG+      E+ I E  + +G+G N+ T LY   GEE+A H Q
Sbjct: 515 SDYILHPFQSVKIFARGQGIANPPEVLIFEGDETFGVGANVQTILYNNKGEERATHIQ 572

 Score = 30.8 bits (68), Expect = 7.0
 Identities = 20/87 (22%), Positives = 36/87 (41%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           +++Q   +R +++DLE +N   E  +  +    E+ QRS E  L     Q+         
Sbjct: 332 LRNQTSELRQKLSDLESRNLLLEKQIEDLNYQLEDDQRSYEAALNDKDAQIRKLREECQA 391

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLE 263
                      K  L+ E+  Y ++LE
Sbjct: 392 LMVELQMLLDTKQTLDGELKVYRQMLE 418

>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
           A3) (IF-A3) (Cel IF A3)
          Length = 581

 Score = 74.7 bits (182), Expect = 4e-13
 Identities = 48/127 (37%), Positives = 67/127 (52%), Gaps = 3/127 (2%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           S R  ++R+ KGN+SI E    G F+ LEN  + K E +    LKR IDG+ + IVFT  
Sbjct: 456 SSRTSYQRSAKGNVSIKEVSPEGKFVILENTHRDKEEPLGDWKLKRKIDGKRE-IVFTFP 514

Query: 798 NKTTLNAGEKITIWARGKKTGKGKELEIEE--DGWGLGENITTSLYGADGEEKAVHYQEL 971
           +   L+  + + I+ARG       E+ + E  D +G G N+ T LY   GEE+A H Q  
Sbjct: 515 SDYILHPVQTVKIFARGNGVANPPEVLVFEGDDTFGAGANVQTILYNNSGEERATHMQRQ 574

Query: 972 QS*NFTS 992
                TS
Sbjct: 575 SQQTTTS 581

>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
           A1) (IF-A1) (Cel IF A1)
          Length = 575

 Score = 73.2 bits (178), Expect = 1e-12
 Identities = 44/118 (37%), Positives = 66/118 (55%), Gaps = 3/118 (2%)
 Frame = +3

Query: 621 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 797
           + R  F+R+ KGN+SI E   +G FI L+N  + K E I    LKR IDG+ +  V+T+ 
Sbjct: 454 ASRQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKREN-VYTLP 512

Query: 798 NKTTLNAGEKITIWARGKKTGKGKELEI--EEDGWGLGENITTSLYGADGEEKAVHYQ 965
               L AG+ + I+AR +      +  +   ED +G G N+ T L+  +GEE+A H Q
Sbjct: 513 RDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 570

 Score = 44.7 bits (104), Expect = 5e-04
 Identities = 27/92 (29%), Positives = 43/92 (46%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           +++Q+  +R ++ADLE +N+  E  +  +    E+ QRS E  L     Q+         
Sbjct: 330 LRTQLSDLRGKLADLESRNSLLEKQIQELNYQLEDDQRSYEAALNDRDSQIRKMREECQA 389

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESR 278
                      K  L+ EIA Y K+LE EE+R
Sbjct: 390 LMVELQMLLDTKQTLDAEIAIYRKMLEGEENR 421

>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
          Length = 615

 Score = 65.5 bits (158), Expect = 3e-10
 Identities = 39/93 (41%), Positives = 51/93 (54%)
 Frame = +3

Query: 3   IKSQIQSMRSRIADLEMQNAEYEHNLNMIRGSYEEKQRSLELELMYFQEQLTGXXXXXXX 182
           +KS +  +R+R+ADLE +NA+ E     +    EEK R  ELE   ++E++T        
Sbjct: 357 LKSNMTEIRNRLADLEARNAQLERTNQDLLRDLEEKDRQNELESCQYKEEITKLRGEMES 416

Query: 183 XXXXXXXXXXAKLGLELEIAAYGKLLEFEESRV 281
                      KL LELEIAAY KLLE EESRV
Sbjct: 417 ILKELQDLMDIKLSLELEIAAYRKLLEGEESRV 449

 Score = 65.1 bits (157), Expect = 3e-10
 Identities = 40/112 (35%), Positives = 60/112 (53%), Gaps = 2/112 (1%)
 Frame = +3

Query: 636 FRRTTKGNISIAETDINGLFITLENIGQKFETITGMTLKRNIDGQADIIVFTIQNKTTLN 815
           ++RT+KG++SI E D  G FI LE   +K E +TG  + R +D    +  + I N   L 
Sbjct: 503 YQRTSKGSVSIKEADSQGCFIALET--KKEENLTGWKIVRKVDDNK-VYTYEIPN-LVLK 558

Query: 816 AGEKITIWARGKKT-GKGKEL-EIEEDGWGLGENITTSLYGADGEEKAVHYQ 965
            G  + IW++  +   +G +L   E D WG G N+ T L    GE+KA + Q
Sbjct: 559 TGTVVKIWSKNHQAQARGDDLVSRENDTWGTGSNVVTILQNEKGEDKANYTQ 610

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,570,060
Number of Sequences: 369166
Number of extensions: 1168874
Number of successful extensions: 3709
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3507
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3660
length of database: 68,354,980
effective HSP length: 112
effective length of database: 47,664,660
effective search space used: 11820835680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

1. Dr_sW_025_G14
2. Dr_sW_007_A23
3. Dr_sW_024_A04
4. Dr_sW_002_N22
5. Dr_sW_011_B09
6. Dr_sW_001_G20
7. Dr_sW_011_H16
8. Dr_sW_013_I04
9. Dr_sW_027_L23
10. Dr_sW_028_M08
11. Dr_sW_002_O10
12. Dr_sW_001_C15
13. Dr_sW_013_K03