Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_B09
(990 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B) 81 4e-15
sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (I... 79 2e-14
sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A) 78 3e-14
sp|P31732|OV71_ONCVO Muscle cell intermediate filament prot... 78 3e-14
sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (I... 75 2e-13
sp|Q17107|AV71_ACAVI Muscle cell intermediate filament prot... 75 2e-13
sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (I... 75 4e-13
sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (I... 75 4e-13
sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (I... 73 1e-12
sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70) 65 3e-10
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
Length = 589
Score = 81.3 bits (199), Expect = 4e-15
Identities = 49/120 (40%), Positives = 70/120 (58%), Gaps = 4/120 (3%)
Frame = +3
Query: 540 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 716
S + F+R+ KGN++I+E D NG FITLEN + K E + LKR +D + + IV+TI
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFITLENTHRSKDENLGEHRLKRKLDNRRE-IVYTIP 523
Query: 717 NKTTLNAGEKITIWARGKKTGKGKELEIEEDG---WGLGENITTSLYGADGEEKAVHYQE 887
T L AG + I+AR + + DG WG+G N+ TSL DG+E+A H Q+
Sbjct: 524 PNTVLKAGRTMKIYARDQGGIHNPPDTLVFDGENTWGIGANVVTSLINKDGDERATHTQK 583
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
B1) (IF-B1) (Cel IF B1)
Length = 589
Score = 79.3 bits (194), Expect = 2e-14
Identities = 47/132 (35%), Positives = 74/132 (56%), Gaps = 4/132 (3%)
Frame = +3
Query: 504 NELSSQEAGAYQSKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNI 680
N S++ S + F+R+ KGN++I+E D NG FI LEN + K E + ++R +
Sbjct: 453 NTDSTRSVRGEVSTKTTFQRSAKGNVTISECDPNGKFIKLENSHRNKDENVGEHKIRRKL 512
Query: 681 DGQADIIVFTIQNKTTLNAGEKITIWARGKKTGKGKELEIEEDG---WGLGENITTSLYG 851
DG+ +I V++I + G+ +TI+AR + + DG WG+G N+ TSL
Sbjct: 513 DGRREI-VYSIPANVVIKPGKNLTIYARDQGGINNPPESLVFDGENTWGIGANVVTSLVN 571
Query: 852 ADGEEKAVHYQE 887
DGEE+A H Q+
Sbjct: 572 KDGEERATHTQK 583
>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
Length = 497
Score = 78.2 bits (191), Expect = 3e-14
Identities = 47/119 (39%), Positives = 70/119 (58%), Gaps = 4/119 (3%)
Frame = +3
Query: 540 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 716
+ R F+R+ KGN+SI ET +G ++ LEN + K E I LKR IDG+ + IV+T+
Sbjct: 374 ASRTSFQRSAKGNVSIQETAPDGRYVVLENTHRSKEEAIGEWKLKRKIDGKRE-IVYTLP 432
Query: 717 NKTTLNAGEKITIWARGKK--TGKGKELEIE-EDGWGLGENITTSLYGADGEEKAVHYQ 884
L G+ + IWARG+ ++L + ED +G G N+ T L+ +GEE+A H Q
Sbjct: 433 RDFILRPGKSVKIWARGQGGIHSPPEQLVFDLEDTFGSGSNVQTILFNREGEERATHIQ 491
>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
Length = 432
Score = 78.2 bits (191), Expect = 3e-14
Identities = 46/118 (38%), Positives = 67/118 (56%), Gaps = 3/118 (2%)
Frame = +3
Query: 540 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 716
+ R F+R+ KGN+SI + +G FI LEN + K E I LKR IDG+ +I V+T
Sbjct: 309 ASRTSFQRSAKGNVSIQDASSDGKFILLENTHRSKEEPIGEWRLKRKIDGKREI-VYTFP 367
Query: 717 NKTTLNAGEKITIWARGKKTGKGKELEI--EEDGWGLGENITTSLYGADGEEKAVHYQ 884
L G+ + IWARG+ + + ED +G+G N+ T L+ +GEE+A H Q
Sbjct: 368 RDFILKPGKTVKIWARGQGVYSPPDQLVFDAEDSFGVGSNVQTILFNKEGEERASHIQ 425
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
A4) (IF-A4) (Cel IF A4)
Length = 575
Score = 75.5 bits (184), Expect = 2e-13
Identities = 42/112 (37%), Positives = 67/112 (59%), Gaps = 2/112 (1%)
Frame = +3
Query: 555 FRRTTKGNISIAETDINGLFITLENIGQKFETITGMTLKRNIDGQADIIVFTIQNKTTLN 734
+ R+ KGNI+I E + +G +I LENI ++ E I L+R I G+ + IVFT + TL
Sbjct: 460 YSRSAKGNIAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAGKRE-IVFTFpreFTLR 518
Query: 735 AGEKITIWARGKKTGKGKELEIE--EDGWGLGENITTSLYGADGEEKAVHYQ 884
A + + I+ARG+ + + ED +G G ++ T+LY +GE++A H Q
Sbjct: 519 AQKSVKIFARGQGVHSPPDSLVYDLEDSFGTGNDVVTTLYNKEGEDRASHSQ 570
>sp|Q17107|AV71_ACAVI Muscle cell intermediate filament protein AV71
Length = 394
Score = 75.5 bits (184), Expect = 2e-13
Identities = 44/118 (37%), Positives = 66/118 (55%), Gaps = 3/118 (2%)
Frame = +3
Query: 540 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 716
+ R F+R+ KGN+SI + +G +I EN + K E I LKR IDG+ +I V+T
Sbjct: 271 ASRTSFQRSAKGNVSIQDAASDGKYILSENTHRSKEEPIGEWRLKRKIDGKREI-VYTFP 329
Query: 717 NKTTLNAGEKITIWARGKKTGKGKELEI--EEDGWGLGENITTSLYGADGEEKAVHYQ 884
L G+ + IWARG+ + + ED +G+G N+ T L+ +GEE+A H Q
Sbjct: 330 TNFVLKPGKSVKIWARGQGVYSPPDQLVFDAEDSFGIGSNVQTILFNKEGEERASHIQ 387
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
A2) (IF-A2) (Cel IF A2)
Length = 581
Score = 74.7 bits (182), Expect = 4e-13
Identities = 46/118 (38%), Positives = 68/118 (57%), Gaps = 3/118 (2%)
Frame = +3
Query: 540 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 716
S R ++R+ KGN++I ET G F+ LEN + K E + LKR IDG+ + IVFT
Sbjct: 456 SSRTSYQRSAKGNVAIKETSPEGKFVILENTHRAKEEPLGDWKLKRKIDGKRE-IVFTFP 514
Query: 717 NKTTLNAGEKITIWARGKKTGKGKELEIEE--DGWGLGENITTSLYGADGEEKAVHYQ 884
+ L+ + + I+ARG+ E+ I E + +G+G N+ T LY GEE+A H Q
Sbjct: 515 SDYILHPFQSVKIFARGQGIANPPEVLIFEGDETFGVGANVQTILYNNKGEERATHIQ 572
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
A3) (IF-A3) (Cel IF A3)
Length = 581
Score = 74.7 bits (182), Expect = 4e-13
Identities = 48/127 (37%), Positives = 67/127 (52%), Gaps = 3/127 (2%)
Frame = +3
Query: 540 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 716
S R ++R+ KGN+SI E G F+ LEN + K E + LKR IDG+ + IVFT
Sbjct: 456 SSRTSYQRSAKGNVSIKEVSPEGKFVILENTHRDKEEPLGDWKLKRKIDGKRE-IVFTFP 514
Query: 717 NKTTLNAGEKITIWARGKKTGKGKELEIEE--DGWGLGENITTSLYGADGEEKAVHYQEL 890
+ L+ + + I+ARG E+ + E D +G G N+ T LY GEE+A H Q
Sbjct: 515 SDYILHPVQTVKIFARGNGVANPPEVLVFEGDDTFGAGANVQTILYNNSGEERATHMQRQ 574
Query: 891 QS*NFTS 911
TS
Sbjct: 575 SQQTTTS 581
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
A1) (IF-A1) (Cel IF A1)
Length = 575
Score = 73.2 bits (178), Expect = 1e-12
Identities = 44/118 (37%), Positives = 66/118 (55%), Gaps = 3/118 (2%)
Frame = +3
Query: 540 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 716
+ R F+R+ KGN+SI E +G FI L+N + K E I LKR IDG+ + V+T+
Sbjct: 454 ASRQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKREN-VYTLP 512
Query: 717 NKTTLNAGEKITIWARGKKTGKGKELEI--EEDGWGLGENITTSLYGADGEEKAVHYQ 884
L AG+ + I+AR + + + ED +G G N+ T L+ +GEE+A H Q
Sbjct: 513 RDFVLRAGKTLKIFARNQGVASPPDQLVYDAEDSFGSGNNVQTILFNKEGEERATHIQ 570
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
Length = 615
Score = 65.1 bits (157), Expect = 3e-10
Identities = 40/112 (35%), Positives = 60/112 (53%), Gaps = 2/112 (1%)
Frame = +3
Query: 555 FRRTTKGNISIAETDINGLFITLENIGQKFETITGMTLKRNIDGQADIIVFTIQNKTTLN 734
++RT+KG++SI E D G FI LE +K E +TG + R +D + + I N L
Sbjct: 503 YQRTSKGSVSIKEADSQGCFIALET--KKEENLTGWKIVRKVDDNK-VYTYEIPN-LVLK 558
Query: 735 AGEKITIWARGKKT-GKGKEL-EIEEDGWGLGENITTSLYGADGEEKAVHYQ 884
G + IW++ + +G +L E D WG G N+ T L GE+KA + Q
Sbjct: 559 TGTVVKIWSKNHQAQARGDDLVSRENDTWGTGSNVVTILQNEKGEDKANYTQ 610
Score = 45.8 bits (107), Expect = 2e-04
Identities = 28/60 (46%), Positives = 32/60 (53%)
Frame = +3
Query: 21 EEKQRSLELELMYFQEQLTGXXXXXXXXXXXXXXXXXAKLGLELEIAAYGKLLEFEESRV 200
EEK R ELE ++E++T KL LELEIAAY KLLE EESRV
Sbjct: 390 EEKDRQNELESCQYKEEITKLRGEMESILKELQDLMDIKLSLELEIAAYRKLLEGEESRV 449
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,985,831
Number of Sequences: 369166
Number of extensions: 1055243
Number of successful extensions: 3038
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2932
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2994
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10431168110
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)