Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_N22
(786 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A) 60 9e-09
sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B) 58 3e-08
sp|P31732|OV71_ONCVO Muscle cell intermediate filament prot... 57 4e-08
sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (I... 56 1e-07
sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (I... 54 4e-07
sp|Q17107|AV71_ACAVI Muscle cell intermediate filament prot... 54 4e-07
sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (I... 54 5e-07
sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (I... 53 8e-07
sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (I... 52 2e-06
sp|P11048|LAMA_XENLA Lamin A 48 3e-05
>sp|P23730|IFEA_ASCSU Intermediate filament protein A (IF-A)
Length = 497
Score = 59.7 bits (143), Expect = 9e-09
Identities = 33/78 (42%), Positives = 48/78 (61%), Gaps = 1/78 (1%)
Frame = +2
Query: 464 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 640
+ R F+R+ KGN+SI ET +G ++ LEN + K E I LKR IDG+ + IV+T+
Sbjct: 374 ASRTSFQRSAKGNVSIQETAPDGRYVVLENTHRSKEEAIGEWKLKRKIDGKRE-IVYTLP 432
Query: 641 NKTTLNAGEKITIWARGK 694
L G+ + IWARG+
Sbjct: 433 RDFILRPGKSVKIWARGQ 450
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
Length = 589
Score = 58.2 bits (139), Expect = 3e-08
Identities = 34/76 (44%), Positives = 48/76 (63%), Gaps = 1/76 (1%)
Frame = +2
Query: 464 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 640
S + F+R+ KGN++I+E D NG FITLEN + K E + LKR +D + + IV+TI
Sbjct: 465 STKTTFQRSAKGNVTISECDPNGKFITLENTHRSKDENLGEHRLKRKLDNRRE-IVYTIP 523
Query: 641 NKTTLNAGEKITIWAR 688
T L AG + I+AR
Sbjct: 524 PNTVLKAGRTMKIYAR 539
>sp|P31732|OV71_ONCVO Muscle cell intermediate filament protein OV71
Length = 432
Score = 57.4 bits (137), Expect = 4e-08
Identities = 33/78 (42%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Frame = +2
Query: 464 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 640
+ R F+R+ KGN+SI + +G FI LEN + K E I LKR IDG+ + IV+T
Sbjct: 309 ASRTSFQRSAKGNVSIQDASSDGKFILLENTHRSKEEPIGEWRLKRKIDGKRE-IVYTFP 367
Query: 641 NKTTLNAGEKITIWARGK 694
L G+ + IWARG+
Sbjct: 368 RDFILKPGKTVKIWARGQ 385
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
A4) (IF-A4) (Cel IF A4)
Length = 575
Score = 55.8 bits (133), Expect = 1e-07
Identities = 30/72 (41%), Positives = 46/72 (63%)
Frame = +2
Query: 479 FRRTTKGNISIAETDINGLFITLENIGQKFETITGMTLKRNIDGQADIIVFTIQNKTTLN 658
+ R+ KGNI+I E + +G +I LENI ++ E I L+R I G+ + IVFT + TL
Sbjct: 460 YSRSAKGNIAIQEAEPSGKYIVLENISRRDENIGDWKLRRKIAGKRE-IVFTFpreFTLR 518
Query: 659 AGEKITIWARGK 694
A + + I+ARG+
Sbjct: 519 AQKSVKIFARGQ 530
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
A2) (IF-A2) (Cel IF A2)
Length = 581
Score = 54.3 bits (129), Expect = 4e-07
Identities = 34/90 (37%), Positives = 51/90 (56%), Gaps = 1/90 (1%)
Frame = +2
Query: 464 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 640
S R ++R+ KGN++I ET G F+ LEN + K E + LKR IDG+ + IVFT
Sbjct: 456 SSRTSYQRSAKGNVAIKETSPEGKFVILENTHRAKEEPLGDWKLKRKIDGKRE-IVFTFP 514
Query: 641 NKTTLNAGEKITIWARGKKTGKGKELEIEE 730
+ L+ + + I+ARG+ E+ I E
Sbjct: 515 SDYILHPFQSVKIFARGQGIANPPEVLIFE 544
>sp|Q17107|AV71_ACAVI Muscle cell intermediate filament protein AV71
Length = 394
Score = 54.3 bits (129), Expect = 4e-07
Identities = 31/78 (39%), Positives = 45/78 (57%), Gaps = 1/78 (1%)
Frame = +2
Query: 464 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 640
+ R F+R+ KGN+SI + +G +I EN + K E I LKR IDG+ + IV+T
Sbjct: 271 ASRTSFQRSAKGNVSIQDAASDGKYILSENTHRSKEEPIGEWRLKRKIDGKRE-IVYTFP 329
Query: 641 NKTTLNAGEKITIWARGK 694
L G+ + IWARG+
Sbjct: 330 TNFVLKPGKSVKIWARGQ 347
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
B1) (IF-B1) (Cel IF B1)
Length = 589
Score = 53.9 bits (128), Expect = 5e-07
Identities = 31/88 (35%), Positives = 52/88 (59%), Gaps = 1/88 (1%)
Frame = +2
Query: 428 NELSSQEAGAYQSKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNI 604
N S++ S + F+R+ KGN++I+E D NG FI LEN + K E + ++R +
Sbjct: 453 NTDSTRSVRGEVSTKTTFQRSAKGNVTISECDPNGKFIKLENSHRNKDENVGEHKIRRKL 512
Query: 605 DGQADIIVFTIQNKTTLNAGEKITIWAR 688
DG+ + IV++I + G+ +TI+AR
Sbjct: 513 DGRRE-IVYSIPANVVIKPGKNLTIYAR 539
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
A3) (IF-A3) (Cel IF A3)
Length = 581
Score = 53.1 bits (126), Expect = 8e-07
Identities = 36/104 (34%), Positives = 53/104 (50%), Gaps = 1/104 (0%)
Frame = +2
Query: 464 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 640
S R ++R+ KGN+SI E G F+ LEN + K E + LKR IDG+ + IVFT
Sbjct: 456 SSRTSYQRSAKGNVSIKEVSPEGKFVILENTHRDKEEPLGDWKLKRKIDGKRE-IVFTFP 514
Query: 641 NKTTLNAGEKITIWARGKKTGKGKELEIEEDGLGFRRKYNYFTV 772
+ L+ + + I+ARG E+ + E F N T+
Sbjct: 515 SDYILHPVQTVKIFARGNGVANPPEVLVFEGDDTFGAGANVQTI 558
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
A1) (IF-A1) (Cel IF A1)
Length = 575
Score = 51.6 bits (122), Expect = 2e-06
Identities = 31/78 (39%), Positives = 46/78 (58%), Gaps = 1/78 (1%)
Frame = +2
Query: 464 SKRMKFRRTTKGNISIAETDINGLFITLENIGQ-KFETITGMTLKRNIDGQADIIVFTIQ 640
+ R F+R+ KGN+SI E +G FI L+N + K E I LKR IDG+ + V+T+
Sbjct: 454 ASRQSFQRSAKGNVSIHEASPDGKFIVLQNTHRAKDEAIGEWKLKRRIDGKRE-NVYTLP 512
Query: 641 NKTTLNAGEKITIWARGK 694
L AG+ + I+AR +
Sbjct: 513 RDFVLRAGKTLKIFARNQ 530
>sp|P11048|LAMA_XENLA Lamin A
Length = 665
Score = 47.8 bits (112), Expect = 3e-05
Identities = 26/82 (31%), Positives = 40/82 (48%)
Frame = +2
Query: 446 EAGAYQSKRMKFRRTTKGNISIAETDINGLFITLENIGQKFETITGMTLKRNIDGQADII 625
E G +S T G +S+ E D G ++ L N + +++ +KR I G I
Sbjct: 418 EEGESRSSSFTQHARTTGKVSVEEVDPEGKYVRLRNKSNEDQSLGNWQIKRQI-GDETPI 476
Query: 626 VFTIQNKTTLNAGEKITIWARG 691
V+ + TL AG+ +TIWA G
Sbjct: 477 VYKFPPRLTLKAGQTVTIWASG 498
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,150,432
Number of Sequences: 369166
Number of extensions: 697867
Number of successful extensions: 2258
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2243
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7405750800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)